Objective To investigate the effect of mucin 1(MUC1)on the proliferation and apoptosis of nasopharyngeal carcinoma(NPC)and its regulatory mechanism.Methods The 60 NPC and paired para-cancer normal tissues were collect...Objective To investigate the effect of mucin 1(MUC1)on the proliferation and apoptosis of nasopharyngeal carcinoma(NPC)and its regulatory mechanism.Methods The 60 NPC and paired para-cancer normal tissues were collected from October 2020 to July 2021 in Quanzhou First Hospital.The expression of MUC1 was measured by real-time quantitative PCR(qPCR)in the patients with PNC.The 5-8F and HNE1 cells were transfected with siRNA control(si-control)or siRNA targeting MUC1(si-MUC1).Cell proliferation was analyzed by cell counting kit-8 and colony formation assay,and apoptosis was analyzed by flow cytometry analysis in the 5-8F and HNE1 cells.The qPCR and ELISA were executed to analyze the levels of TNF-αand IL-6.Western blot was performed to measure the expression of MUC1,NFкB and apoptosis-related proteins(Bax and Bcl-2).Results The expression of MUC1 was up-regulated in the NPC tissues,and NPC patients with the high MUC1 expression were inclined to EBV infection,growth and metastasis of NPC.Loss of MUC1 restrained malignant features,including the proliferation and apoptosis,downregulated the expression of p-IкB、p-P65 and Bcl-2 and upregulated the expression of Bax in the NPC cells.Conclusion Downregulation of MUC1 restrained biological characteristics of malignancy,including cell proliferation and apoptosis,by inactivating NF-κB signaling pathway in NPC.展开更多
Background: Fusobacterium nucleatum can cause opportunistic and chronic infections and has recently been shown to be involved in colorectal cancer. However, the specific mechanism by which F. nucleatum induces colorect...Background: Fusobacterium nucleatum can cause opportunistic and chronic infections and has recently been shown to be involved in colorectal cancer. However, the specific mechanism by which F. nucleatum induces colorectal carcinoma remains unclear. Methods: We downloaded the GSE110223, GSE110224, GSE113513 and GSE122183 microarray datasets from the Gene Expression Omnibus (GEO) database. Identification of differentially expressed genes (DEGs) related to F. nucleatum in CRC by overlapping data sets was performed. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genome pathway (KEGG) analyses were used for enrichment analysis. Moreover, Cytoscape software constructed a protein-protein interaction (PPI) network of differentially expressed genes. Finally, western blot and RT-qPCR analysis identified the relative protein and mRNA expression of hub genes in the cell model. Results: In total, 118 DEGs in F. nucleatum-associated CRC were screened from nonoverlapping microarray data, among which 20 upregulated and 98 downregulated DEGs were identified. The 118 DEGs were significantly correlated with diverse functions and pathways. The hub gene MUC1 had higher centrality scores in the PPI network, and the top 5 closely interacting hub genes, SLC7A11, AGR2, KRT18, CARTPT and TSPYL5, were identified. Conclusion: Our evidence suggests that the identified DEGs associated with F. nucleatum enhance our comprehension of the molecular Mechanisms underlying the tumorigenesis and development of CRC and might be used as molecular targets and diagnostic biomarkers for the treatment of CRC.展开更多
目的通过Meta分析探讨甲状腺癌组织中黏蛋白1(MUC1)表达的临床意义及其与各临床病理参数的相关性。方法检索维普、万方、中国知网、中国生物医学文献、PubMed、Web of Science、Embase、Cochrane中英文数据库中关于MUC1在甲状腺癌中表...目的通过Meta分析探讨甲状腺癌组织中黏蛋白1(MUC1)表达的临床意义及其与各临床病理参数的相关性。方法检索维普、万方、中国知网、中国生物医学文献、PubMed、Web of Science、Embase、Cochrane中英文数据库中关于MUC1在甲状腺癌中表达及其意义的文献,以比值比(OR)和95%置信区间(95%CI)为效应指标,采用STATA 14软件进行Meta分析,并对发表偏倚及敏感性进行检验。结果共纳入13项病例对照研究,甲状腺癌病例组共1157例,非甲状腺癌对照组共509例。结果显示,甲状腺癌病例组MUC1表达明显高于非甲状腺癌对照组(OR=9.78,95%CI:7.49~12.78,P<0.001);伴有淋巴结转移的甲状腺癌组织中MUC1表达高于无淋巴结转移的甲状腺癌组织(OR=3.08,95%CI:1.37~6.92,P<0.05);TNM分期为Ⅲ~Ⅳ期的甲状腺癌组织中MUC1表达高于TNM分期为Ⅰ~Ⅱ期的甲状腺癌组织(OR=1.88,95%CI:1.22~2.90,P<0.05)。结论与非甲状腺癌对照组比较,甲状腺癌病例组MUC1呈高表达,且MUC1高表达与淋巴结转移、TNM分期密切相关。MUC1与甲状腺癌的发生和发展存在相关性,有望成为甲状腺癌分子研究领域的一项新靶标。展开更多
文摘Objective To investigate the effect of mucin 1(MUC1)on the proliferation and apoptosis of nasopharyngeal carcinoma(NPC)and its regulatory mechanism.Methods The 60 NPC and paired para-cancer normal tissues were collected from October 2020 to July 2021 in Quanzhou First Hospital.The expression of MUC1 was measured by real-time quantitative PCR(qPCR)in the patients with PNC.The 5-8F and HNE1 cells were transfected with siRNA control(si-control)or siRNA targeting MUC1(si-MUC1).Cell proliferation was analyzed by cell counting kit-8 and colony formation assay,and apoptosis was analyzed by flow cytometry analysis in the 5-8F and HNE1 cells.The qPCR and ELISA were executed to analyze the levels of TNF-αand IL-6.Western blot was performed to measure the expression of MUC1,NFкB and apoptosis-related proteins(Bax and Bcl-2).Results The expression of MUC1 was up-regulated in the NPC tissues,and NPC patients with the high MUC1 expression were inclined to EBV infection,growth and metastasis of NPC.Loss of MUC1 restrained malignant features,including the proliferation and apoptosis,downregulated the expression of p-IкB、p-P65 and Bcl-2 and upregulated the expression of Bax in the NPC cells.Conclusion Downregulation of MUC1 restrained biological characteristics of malignancy,including cell proliferation and apoptosis,by inactivating NF-κB signaling pathway in NPC.
文摘Background: Fusobacterium nucleatum can cause opportunistic and chronic infections and has recently been shown to be involved in colorectal cancer. However, the specific mechanism by which F. nucleatum induces colorectal carcinoma remains unclear. Methods: We downloaded the GSE110223, GSE110224, GSE113513 and GSE122183 microarray datasets from the Gene Expression Omnibus (GEO) database. Identification of differentially expressed genes (DEGs) related to F. nucleatum in CRC by overlapping data sets was performed. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genome pathway (KEGG) analyses were used for enrichment analysis. Moreover, Cytoscape software constructed a protein-protein interaction (PPI) network of differentially expressed genes. Finally, western blot and RT-qPCR analysis identified the relative protein and mRNA expression of hub genes in the cell model. Results: In total, 118 DEGs in F. nucleatum-associated CRC were screened from nonoverlapping microarray data, among which 20 upregulated and 98 downregulated DEGs were identified. The 118 DEGs were significantly correlated with diverse functions and pathways. The hub gene MUC1 had higher centrality scores in the PPI network, and the top 5 closely interacting hub genes, SLC7A11, AGR2, KRT18, CARTPT and TSPYL5, were identified. Conclusion: Our evidence suggests that the identified DEGs associated with F. nucleatum enhance our comprehension of the molecular Mechanisms underlying the tumorigenesis and development of CRC and might be used as molecular targets and diagnostic biomarkers for the treatment of CRC.
文摘目的通过Meta分析探讨甲状腺癌组织中黏蛋白1(MUC1)表达的临床意义及其与各临床病理参数的相关性。方法检索维普、万方、中国知网、中国生物医学文献、PubMed、Web of Science、Embase、Cochrane中英文数据库中关于MUC1在甲状腺癌中表达及其意义的文献,以比值比(OR)和95%置信区间(95%CI)为效应指标,采用STATA 14软件进行Meta分析,并对发表偏倚及敏感性进行检验。结果共纳入13项病例对照研究,甲状腺癌病例组共1157例,非甲状腺癌对照组共509例。结果显示,甲状腺癌病例组MUC1表达明显高于非甲状腺癌对照组(OR=9.78,95%CI:7.49~12.78,P<0.001);伴有淋巴结转移的甲状腺癌组织中MUC1表达高于无淋巴结转移的甲状腺癌组织(OR=3.08,95%CI:1.37~6.92,P<0.05);TNM分期为Ⅲ~Ⅳ期的甲状腺癌组织中MUC1表达高于TNM分期为Ⅰ~Ⅱ期的甲状腺癌组织(OR=1.88,95%CI:1.22~2.90,P<0.05)。结论与非甲状腺癌对照组比较,甲状腺癌病例组MUC1呈高表达,且MUC1高表达与淋巴结转移、TNM分期密切相关。MUC1与甲状腺癌的发生和发展存在相关性,有望成为甲状腺癌分子研究领域的一项新靶标。
