应用免疫组织化学和原位杂交方法检测人正常腺上皮中 MU C6基因的表达 ,揭示 MU C6基因在正常人腺上皮组织中的分布异质性及其特点。结果显示 :MU C6基因编码的核心蛋白及其 m RNA主要分布于正常胃粘膜胃腺的基底部 ,上皮细胞无 MU C6...应用免疫组织化学和原位杂交方法检测人正常腺上皮中 MU C6基因的表达 ,揭示 MU C6基因在正常人腺上皮组织中的分布异质性及其特点。结果显示 :MU C6基因编码的核心蛋白及其 m RNA主要分布于正常胃粘膜胃腺的基底部 ,上皮细胞无 MU C6基因表达 ,呈细颗粒状 ,位于细胞核周 ,胃底、胃窦的表达无区别 ;十二指肠绒毛上皮内的表达呈弥漫性 ,均质状 ,杯状和柱状细胞的表达类似 ,杯状细胞的粘液滴内未测得 MUC6基因产物 ;空肠、结肠组织中无 MUC6基因的表达 ;胆囊上皮组织内有强阳性 MUC6核心蛋白的表达 ,而宫颈上皮中表达较弱。实验提示展开更多
AIM： To assess histochemical expression of KL-6 and its clinicopathological significance in carcinoma of the ampulla of Vater. METHODS： Ampullary carcinoma tissues were collected from 38 patients who underwent pancr...AIM： To assess histochemical expression of KL-6 and its clinicopathological significance in carcinoma of the ampulla of Vater. METHODS： Ampullary carcinoma tissues were collected from 38 patients who underwent pancreatoduodenectomy or local resection. Tissues were subjected to immunohistochemical analysis using KL-6 antibody. RESULTS： Positive staining of ampullary carcinoma cells was observed in 26 （68.4%） cases. Staining was not found in the surrounding non-cancer regions of the ampullary tissues. Remarkable KL-6 expression was observed in invasive carcinoma cells in pancreatic and duodenal tissues and in metastatic carcinoma cells in lymph nodes. Positive KL-6 expression was related to lymph node metastasis （P = 0.020）, pancreatic invasion （P = 0.016）, duodenal invasion （P = 0.034）, and advanced stage of TNM clinical classification （P = 0.010）. Survival analysis showed that positive expression of KL-6 was related to a poorer prognosis （P = 0.029）. CONCLUSION： The aberrant expression of KL-6 mucin is significantly related to unfavorable behaviors of cardnoma of the ampulla of Vater.展开更多
AIM： To assess subcellular localization of KL-6 mucin and its clinicopathological significance in colorectal carcinoma as well as metastatic lymph node and liver tissues. METHODS： Colorectal carcinoma tissues as wel...AIM： To assess subcellular localization of KL-6 mucin and its clinicopathological significance in colorectal carcinoma as well as metastatic lymph node and liver tissues. METHODS： Colorectal carcinoma tissues as well as metastatic lymph node and liver tissues were collected from 82 patients who underwent colorectomy or hepatectomy. Tissues were subjected to immunohistochemical analysis using KL-6 antibody. RESULTS： Of the 82 colorectal carcinoma patients, 6 showed no staining, 29 showed positive staining only in the apical membrane, and 47 showed positive staining in the circumferential membrane and/or cytoplasm. Positive staining was not observed in non-cancerous colorectal epithelial cells surrounding the tumor tissues. The five-year survival rate was significantly lower in cases showing positive staining in the circumferential membrane and/or cytoplasm （63.0%） than those showing positive staining only in the apical membrane （85.7%） and those showing no staining （100%）. Statistical analysis between clinicopathological factors and subcellular localization of KL-6 mucin showed that KL-6 localization in the circumferential membrane and/or cytoplasm was significantly associated with the presence of venous invasion （P = 0.0003）, lymphatic invasion （P 〈 0.0001）, lymph node metastasis （P〈0.0001）, liver metastasis （P = 0.058）, and advanced histological stage （P〈 0.0001）. Positive staining was observed in all metastatic lesions tested as well as in the primary colorectal carcinoma tissues. CONCLUSION： The subcellular staining pattern of KL-6 in colorectal adenocarcinoma may be an important indicator for unfavorable behaviors such as lymph node and liver metastasis, as well as for the prognosis of patients.展开更多
AIM： To investigate the relationship between mucin 6 （MUC6） VNTR length and Hpylori infection. METHODS： Blood samples were collected from patients visiting the Can Tho General Hospital for upper gastrointestinal e...AIM： To investigate the relationship between mucin 6 （MUC6） VNTR length and Hpylori infection. METHODS： Blood samples were collected from patients visiting the Can Tho General Hospital for upper gastrointestinal endoscopy. DNA was isolated from whole blood, the repeated section was cut out using a restriction enzyme （Pvu 11） and the length of the allele fragments was determined by Southern blotting. Hpylori infection was diagnosed by ^14C urea breath test. For analysis, MUC6 allele fragment length was dichotomized as being either long （〉 13.5 kbp） or short （≤ 13.5 kbp） and patients were classified according to genotype [long- long （LL）, long-short （LS）, short-short （SS）]. RESULTS： 160 patients were studied （mean age 43 years, 36% were males, 58% H pylori positive）. MUC6 PvuⅡ-restricted allele fragment lengths ranged from 7 to 19 kbp. Of the patients with the LL, LS, SS MUC6 genotype, 43% （24/56）, 57% （25/58） and 76% （11/46） were infected with Hpylori, respectively （P = 0.003）. CONCLUSION： Short MUC6 alleles are associated with H pylori infection.展开更多
基金Supported by Grants-in-aid From the Ministry of Education, Science, Sports, and Culture of Japan and a grant for Hi-Tech Research from Tokai University
文摘AIM： To assess histochemical expression of KL-6 and its clinicopathological significance in carcinoma of the ampulla of Vater. METHODS： Ampullary carcinoma tissues were collected from 38 patients who underwent pancreatoduodenectomy or local resection. Tissues were subjected to immunohistochemical analysis using KL-6 antibody. RESULTS： Positive staining of ampullary carcinoma cells was observed in 26 （68.4%） cases. Staining was not found in the surrounding non-cancer regions of the ampullary tissues. Remarkable KL-6 expression was observed in invasive carcinoma cells in pancreatic and duodenal tissues and in metastatic carcinoma cells in lymph nodes. Positive KL-6 expression was related to lymph node metastasis （P = 0.020）, pancreatic invasion （P = 0.016）, duodenal invasion （P = 0.034）, and advanced stage of TNM clinical classification （P = 0.010）. Survival analysis showed that positive expression of KL-6 was related to a poorer prognosis （P = 0.029）. CONCLUSION： The aberrant expression of KL-6 mucin is significantly related to unfavorable behaviors of cardnoma of the ampulla of Vater.
基金Supported by Grants-in-aid from the Ministry of Education,Science,Sports and Culture of Japan and a grant for Hi-Tech Research from Tokai University
文摘AIM： To assess subcellular localization of KL-6 mucin and its clinicopathological significance in colorectal carcinoma as well as metastatic lymph node and liver tissues. METHODS： Colorectal carcinoma tissues as well as metastatic lymph node and liver tissues were collected from 82 patients who underwent colorectomy or hepatectomy. Tissues were subjected to immunohistochemical analysis using KL-6 antibody. RESULTS： Of the 82 colorectal carcinoma patients, 6 showed no staining, 29 showed positive staining only in the apical membrane, and 47 showed positive staining in the circumferential membrane and/or cytoplasm. Positive staining was not observed in non-cancerous colorectal epithelial cells surrounding the tumor tissues. The five-year survival rate was significantly lower in cases showing positive staining in the circumferential membrane and/or cytoplasm （63.0%） than those showing positive staining only in the apical membrane （85.7%） and those showing no staining （100%）. Statistical analysis between clinicopathological factors and subcellular localization of KL-6 mucin showed that KL-6 localization in the circumferential membrane and/or cytoplasm was significantly associated with the presence of venous invasion （P = 0.0003）, lymphatic invasion （P 〈 0.0001）, lymph node metastasis （P〈0.0001）, liver metastasis （P = 0.058）, and advanced histological stage （P〈 0.0001）. Positive staining was observed in all metastatic lesions tested as well as in the primary colorectal carcinoma tissues. CONCLUSION： The subcellular staining pattern of KL-6 in colorectal adenocarcinoma may be an important indicator for unfavorable behaviors such as lymph node and liver metastasis, as well as for the prognosis of patients.
文摘AIM： To investigate the relationship between mucin 6 （MUC6） VNTR length and Hpylori infection. METHODS： Blood samples were collected from patients visiting the Can Tho General Hospital for upper gastrointestinal endoscopy. DNA was isolated from whole blood, the repeated section was cut out using a restriction enzyme （Pvu 11） and the length of the allele fragments was determined by Southern blotting. Hpylori infection was diagnosed by ^14C urea breath test. For analysis, MUC6 allele fragment length was dichotomized as being either long （〉 13.5 kbp） or short （≤ 13.5 kbp） and patients were classified according to genotype [long- long （LL）, long-short （LS）, short-short （SS）]. RESULTS： 160 patients were studied （mean age 43 years, 36% were males, 58% H pylori positive）. MUC6 PvuⅡ-restricted allele fragment lengths ranged from 7 to 19 kbp. Of the patients with the LL, LS, SS MUC6 genotype, 43% （24/56）, 57% （25/58） and 76% （11/46） were infected with Hpylori, respectively （P = 0.003）. CONCLUSION： Short MUC6 alleles are associated with H pylori infection.
