Evaluation of L-dopa,proximate composition with in vitro anti-inflammatory and antioxidant activity of Mucuna macrocarpa beans:A future drug for Parkinson treatment

Objective: To investigate L-3, 4-dihydroxyphenylalanine(L-dopa, anti-Parkinson drug),anti-inflammatory activity, proximate nutritional composition and antioxidant potential of Mucuna macrocarpa(M. macrocarpa) beans.Methods: L-dopa content was determined and quantified by high performance thin layer chromatography and reversed phase high-performance liquid chromatography(RPHPLC) methods. Anti-inflammatory activity was performed by in vitro protein denaturation inhibition and human red blood cell membrane stabilisation activity. Proximate composition and elemental analysis were also investigated. The antioxidant potential(2,2-diphenyl-1-picrylhydrazyl, N-N-dimethyl-phenylenediamine and ferric-reducing antioxidant power) of M. macrocarpa beans were evaluated by using different extraction solvents. The RP-HPLC analysis also quantified significant phenolics such as gallic acid, tannic acid, p-hydroxybenzoic acid and p-coumaric acid.Results: RP-HPLC quantification revealed that M. macrocarpa beans contain a high level of L-dopa [(115.41 ± 0.985) mg/g] which was the highest among the Mucuna species from Indian sub-continent. Water extract of seed powder showed strong antiinflammatory and antioxidant potential. Proximate composition of M. macrocarpa beans revealed numerous nutritional and anti-nutritional components. RP-HPLC analysis of major phenolics such as tannic acid(43.795 mg/g), gallic acid(0.864 mg/g), p-coumaric acid(0.364 mg/g) and p-hydroxybenzoic acid(0.036 mg/g) quantified successfully from M. macrocarpa beans respectively.Conclusions: This study suggests that M. macrocarpa is a potential source of L-dopa with promising anti-inflammatory, antioxidant and nutritional benefits.

HPLC法测定藜豆中左旋多巴的含量

采用HPLC法建立测定藜豆中左旋多巴含量的方法。色谱柱为岛津C-18甲基硅烷柱(250 min×4.6mm,5μm),流动相为甲醇:0.1 mol／L醋酸溶液(25:75,v／v),以硫唑嘌呤为内标物,检测波长280 nm,流速1.0 mL／min,柱温为室温,供试品的前处理方法为微波辅助提取法。结果表明,该方法准确度高,线性关系好,重现性好,平均加样回收率为97.34％,RSD 1.01％(n=5),结果满意。

从藜豆中提取天然黑色素的工艺研究

以我国南方资源丰富的藜豆为原料,对藜豆中的天然黑色素提取工艺进行了研究,得出了藜豆黑色素的最佳提取条件:用4%NaOH溶液于60℃超声浸提40 min,调节pH=2,析出黑色素,得率为3.62%。由紫外和红外波谱分析藜豆黑色素具有邻苯二酚型结构。

2种外源植物激素对大果油麻藤生长和叶绿素荧光参数的影响

以大果油麻藤幼苗为试验材料,采用完全随机区组试验设计,研究茉莉酸甲酯(MeJA)和水杨酸(SA)2种外源植物激素不同浓度处理下的大果油麻藤生长和叶绿素荧光参数的变化,通过隶属函数分析法对不同处理下大果油麻藤各项指标进行综合评价,筛选出对大果油麻藤苗木生长发育起促进作用的外源植物激素及其浓度。结果表明:各处理对地径增长量的影响作用不显著;在0.9和1.3 mmol·L^(-1) MeJA、0.1和0.5 mmol·L^(-1) SA的分别处理下,叶面积和地上地下生物量显著高于其他处理;0.9 mmol·L^(-1) MeJA处理下,叶片中的叶绿素a和叶绿素b含量达到最高,0.5 mmol·L^(-1) SA处理次之;0.9 mmol·L^(-1)、1.3 mmol·L^(-1) MeJA及0.1 mmol·L^(-1) SA处理更有利于PSⅡ反应中心能量捕捉效率的提高,使植株发挥更大的光合效能。综合分析各指标得出,适宜大果油麻藤生长的最佳外源植物激素为0.9 mmol·L^(-1) MeJA,此浓度下大果油麻藤的生长能力最强,综合质量最佳;若选择施用水杨酸,0.5 mmol·L^(-1) SA处理对提高大果油麻藤的生长能力作用明显。