This study aims to evaluate the preventive effects of anthocyanins extracts (MAEs) from mulberry variety PR-01 against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in rats. It was found that 150 mg·k...This study aims to evaluate the preventive effects of anthocyanins extracts (MAEs) from mulberry variety PR-01 against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in rats. It was found that 150 mg·kg-1 MAEs treatment significantly reduced the NDEA-induced hepatic nodules incidence and hepatocellular carcinoma incidence by 58.30% and 41.70% compared to the model group. Meanwhile, MAEs significantly restored the elevated the liver function enzymes, inhibited the tumor necrosis factor alpha and interleukin-6 levels, elevated the serum interleukin-10 and interferon-γ and increased hepatic glutathione-S-transferase and UDP-glucuronosyltransferase 2B1 enzyme activity. Moreover, 150 mg·kg-1 MAEs supplement enhanced glutathione content and the activities of superoxide dismutase, catalase, glutathione peroxidase activities but reduced the malondialdehyde and thiobarbituric acid-reactive substances content by 37.90% and 44.52%. Furthermore, MAEs pretreatment maintained nuclear factor erythroid 2-related factor 2 (Nrf2), Kelch-like ECH-associated protein 1, heme oxygenase-1, and NAD(P)H: quinine oxidoreductase1 stimulation and inhibited the expression of TNF-α, nuclear factor-kappaB (NF-κB), and cyclooxygenase-2 (COX-2), indicating that MAEs exhibit effectively prevention effects against liver cancer via decreased lipid peroxidation, induced Nrf2-mediated antioxidant enzymes and attenuating the inflammatory mediators COX-2 through NF-κB pathway. Thus, MAEs of mulberry variety PR-01 may be used as a good functional dietary supplement against liver cancer.展开更多
为揭示叶果间的内在作用规律,以桑树(Morus alba L.)当年生果枝为研究对象,分析不同大小果枝上叶片特征和果实性状的差异及其二者间的相互关系。结果表明:随着果枝由小到大,果枝上的叶片数与总叶干重显著增加,而叶形指数却无显著变化;...为揭示叶果间的内在作用规律,以桑树(Morus alba L.)当年生果枝为研究对象,分析不同大小果枝上叶片特征和果实性状的差异及其二者间的相互关系。结果表明:随着果枝由小到大,果枝上的叶片数与总叶干重显著增加,而叶形指数却无显著变化;桑葚数与总桑葚干重也有显著增加,桑葚含水量无显著变化。桑葚数与叶片数、总叶干重、总叶面积及其平均桑葚干重显著正相关。研究结果证实了果实的性状与叶片特征之间存在极密切关系。当年生果枝上的桑葚数量多少取决于果枝上的叶片总数,平均桑葚的干重则与果枝上的平均叶面积显著相关。展开更多
Ten quantitative morphological characters were studied in 56 Morus alba L. trees representing three natural populations from the trans-Himalayan Ladakh region. The altitude of collection sites ranged from 2815 to 3177...Ten quantitative morphological characters were studied in 56 Morus alba L. trees representing three natural populations from the trans-Himalayan Ladakh region. The altitude of collection sites ranged from 2815 to 3177 m above the sea level(asl). Coefficient of variation(CV) showed high phenotypic variation in M. alba. Linear regression analysis revealed that leaf and fruit size decreases with an increase in altitude. High CV was observed for leaf length, leaf width, petiole length, leaf area, internodal distance, number of nodes, bud length, fruit length, fruit width and fruit weight. Similarly, a high phenotypic plasticity index was observed for bud length, leaf length, leaf width, petiole length, leaf area, inter-nodal distance, number of nodes, fruit length, fruit width and fruit weight. For every 100 m increase in altitude, leaf length, leaf width and leaf area decreased by 1 cm, 0.8 cm and 16.6 cm2, respectively. Analysis of covariance showed a predominant altitudinal effect on the morphological characters in comparison to the population effect. A small change in the altitude caused significant change in the plant morphological characteristics. The present investigation represents to our knowledge the first study addressing phenotypic variation in mulberryalong an altitudinal gradient.展开更多
Morus alba(white mulberry) mesophyll protoplasts were isolated from leaves of 30-45 day old sterile shoots,with protoplast yields of 2.5 x 107 g-1/F.W. after purification. The protoplasts were cultured in a modified K...Morus alba(white mulberry) mesophyll protoplasts were isolated from leaves of 30-45 day old sterile shoots,with protoplast yields of 2.5 x 107 g-1/F.W. after purification. The protoplasts were cultured in a modified K8P liquid medium containing 0.2 mg/L 2,4-D(2,4- Dichlorophe-noxy acetic acid), 1 mg/L NAA(Naphthyl acetic acid) and 0.5 mg/L BA(6-benzylaminopurine). A low plating density (5 x 104/ml) proved to be favourable to the division of protoplast-derived cells. The first divisioll occurred 4 days after culture, and the division frequency reached 24% at 10 days. A number of cell colonies and microcalli formed in 6 weeks. The microcalli were transferred onto MSB medium with 0.5 mg/L NAA and 0.5 mg/L BA for further proliferation. Shoot formation was initiated when the calli of 3-4 mm in size were transferred onto MSB differentiation medium with 0.1 mg/L NAA and 1 mg/L BA. The frequency of shoot formation was 35%. The shoots of 4-5 cm in height were excised from the callus and rooted on half strength MS medium with 0.5 mg/L IBA and 0.1 mg/L BA. After transplantation into pots, the regenerated plants grew vigorously in the phytotron.展开更多
桑树(Morus alba L.)是非常重要的经济栽培林木之一。近年来,随着人们对生态环境日益恶化的重视,桑树以其对恶劣自然环境的超强适应性和生态治理能力而备受关注。根据森林生态系统服务功能评估规范,并结合桑树生态功能研究现状,将桑树...桑树(Morus alba L.)是非常重要的经济栽培林木之一。近年来,随着人们对生态环境日益恶化的重视,桑树以其对恶劣自然环境的超强适应性和生态治理能力而备受关注。根据森林生态系统服务功能评估规范,并结合桑树生态功能研究现状,将桑树生态系统服务功能归纳为8个指标类型:提供原材料、涵养水源、土壤保育、调节气候、净化空气、维持生物多样性、休闲游憩和文化传承;并就各功能指标研究情况进行了综述。展开更多
针对湖北省桑园立地条件差,肥料使用不科学,造成土壤营养元素缺乏和不平衡等问题,开展测土配方优化施肥试验示范.通过对郧西县六郎乡罗坡垭村桑园土壤肥力的分析发现,土壤养分含量中等,表现为极缺碱解氮,进而制订了不施肥、习惯施肥(全...针对湖北省桑园立地条件差,肥料使用不科学,造成土壤营养元素缺乏和不平衡等问题,开展测土配方优化施肥试验示范.通过对郧西县六郎乡罗坡垭村桑园土壤肥力的分析发现,土壤养分含量中等,表现为极缺碱解氮,进而制订了不施肥、习惯施肥(全年尿素750kg/hm^2)和增量施肥(全年尿素1500kg/hm^2)3个施肥处理方案.结果表明,增量施肥明显提高了桑树(Morus alba L.)的抽枝能力,促进了桑树枝条的伸长生长,单株产叶量、公顷产叶量及桑叶品质明显提高,桑树的抗旱能力得到改善.得出了气候条件(降雨量)是影响桑树养分吸收量及桑园养分利用率的重要因子结论.展开更多
文摘This study aims to evaluate the preventive effects of anthocyanins extracts (MAEs) from mulberry variety PR-01 against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in rats. It was found that 150 mg·kg-1 MAEs treatment significantly reduced the NDEA-induced hepatic nodules incidence and hepatocellular carcinoma incidence by 58.30% and 41.70% compared to the model group. Meanwhile, MAEs significantly restored the elevated the liver function enzymes, inhibited the tumor necrosis factor alpha and interleukin-6 levels, elevated the serum interleukin-10 and interferon-γ and increased hepatic glutathione-S-transferase and UDP-glucuronosyltransferase 2B1 enzyme activity. Moreover, 150 mg·kg-1 MAEs supplement enhanced glutathione content and the activities of superoxide dismutase, catalase, glutathione peroxidase activities but reduced the malondialdehyde and thiobarbituric acid-reactive substances content by 37.90% and 44.52%. Furthermore, MAEs pretreatment maintained nuclear factor erythroid 2-related factor 2 (Nrf2), Kelch-like ECH-associated protein 1, heme oxygenase-1, and NAD(P)H: quinine oxidoreductase1 stimulation and inhibited the expression of TNF-α, nuclear factor-kappaB (NF-κB), and cyclooxygenase-2 (COX-2), indicating that MAEs exhibit effectively prevention effects against liver cancer via decreased lipid peroxidation, induced Nrf2-mediated antioxidant enzymes and attenuating the inflammatory mediators COX-2 through NF-κB pathway. Thus, MAEs of mulberry variety PR-01 may be used as a good functional dietary supplement against liver cancer.
文摘为揭示叶果间的内在作用规律,以桑树(Morus alba L.)当年生果枝为研究对象,分析不同大小果枝上叶片特征和果实性状的差异及其二者间的相互关系。结果表明:随着果枝由小到大,果枝上的叶片数与总叶干重显著增加,而叶形指数却无显著变化;桑葚数与总桑葚干重也有显著增加,桑葚含水量无显著变化。桑葚数与叶片数、总叶干重、总叶面积及其平均桑葚干重显著正相关。研究结果证实了果实的性状与叶片特征之间存在极密切关系。当年生果枝上的桑葚数量多少取决于果枝上的叶片总数,平均桑葚的干重则与果枝上的平均叶面积显著相关。
基金supported by Defence Research and Development Organization (DRDO),Ministry of Defence, Government of India
文摘Ten quantitative morphological characters were studied in 56 Morus alba L. trees representing three natural populations from the trans-Himalayan Ladakh region. The altitude of collection sites ranged from 2815 to 3177 m above the sea level(asl). Coefficient of variation(CV) showed high phenotypic variation in M. alba. Linear regression analysis revealed that leaf and fruit size decreases with an increase in altitude. High CV was observed for leaf length, leaf width, petiole length, leaf area, internodal distance, number of nodes, bud length, fruit length, fruit width and fruit weight. Similarly, a high phenotypic plasticity index was observed for bud length, leaf length, leaf width, petiole length, leaf area, inter-nodal distance, number of nodes, fruit length, fruit width and fruit weight. For every 100 m increase in altitude, leaf length, leaf width and leaf area decreased by 1 cm, 0.8 cm and 16.6 cm2, respectively. Analysis of covariance showed a predominant altitudinal effect on the morphological characters in comparison to the population effect. A small change in the altitude caused significant change in the plant morphological characteristics. The present investigation represents to our knowledge the first study addressing phenotypic variation in mulberryalong an altitudinal gradient.
文摘Morus alba(white mulberry) mesophyll protoplasts were isolated from leaves of 30-45 day old sterile shoots,with protoplast yields of 2.5 x 107 g-1/F.W. after purification. The protoplasts were cultured in a modified K8P liquid medium containing 0.2 mg/L 2,4-D(2,4- Dichlorophe-noxy acetic acid), 1 mg/L NAA(Naphthyl acetic acid) and 0.5 mg/L BA(6-benzylaminopurine). A low plating density (5 x 104/ml) proved to be favourable to the division of protoplast-derived cells. The first divisioll occurred 4 days after culture, and the division frequency reached 24% at 10 days. A number of cell colonies and microcalli formed in 6 weeks. The microcalli were transferred onto MSB medium with 0.5 mg/L NAA and 0.5 mg/L BA for further proliferation. Shoot formation was initiated when the calli of 3-4 mm in size were transferred onto MSB differentiation medium with 0.1 mg/L NAA and 1 mg/L BA. The frequency of shoot formation was 35%. The shoots of 4-5 cm in height were excised from the callus and rooted on half strength MS medium with 0.5 mg/L IBA and 0.1 mg/L BA. After transplantation into pots, the regenerated plants grew vigorously in the phytotron.
文摘桑树(Morus alba L.)是非常重要的经济栽培林木之一。近年来,随着人们对生态环境日益恶化的重视,桑树以其对恶劣自然环境的超强适应性和生态治理能力而备受关注。根据森林生态系统服务功能评估规范,并结合桑树生态功能研究现状,将桑树生态系统服务功能归纳为8个指标类型:提供原材料、涵养水源、土壤保育、调节气候、净化空气、维持生物多样性、休闲游憩和文化传承;并就各功能指标研究情况进行了综述。
文摘针对湖北省桑园立地条件差,肥料使用不科学,造成土壤营养元素缺乏和不平衡等问题,开展测土配方优化施肥试验示范.通过对郧西县六郎乡罗坡垭村桑园土壤肥力的分析发现,土壤养分含量中等,表现为极缺碱解氮,进而制订了不施肥、习惯施肥(全年尿素750kg/hm^2)和增量施肥(全年尿素1500kg/hm^2)3个施肥处理方案.结果表明,增量施肥明显提高了桑树(Morus alba L.)的抽枝能力,促进了桑树枝条的伸长生长,单株产叶量、公顷产叶量及桑叶品质明显提高,桑树的抗旱能力得到改善.得出了气候条件(降雨量)是影响桑树养分吸收量及桑园养分利用率的重要因子结论.
