Anthocyanins from black bean canning wastewater were isolated and purified on a laboratory scale by column chromatography, and then identified by high performance liquid chromatography electrospray tandem mass spectro...Anthocyanins from black bean canning wastewater were isolated and purified on a laboratory scale by column chromatography, and then identified by high performance liquid chromatography electrospray tandem mass spectrometry. Dynamic adsorption and desorption were performed in glass columns packed with Sepabead Sp700 to optimize the purification process. Different temperatures during adsorption and desorption (25℃and 35℃) did not significantly affect the adsorption and desorption ratio. The adsorption ratio was significantly reduced when the flow rate increased from 1.5 mL/min to 2.5 mL/min. However, desorption ratio was not affected by flow rate (from 1.5 mL/min to 0.3 mL/min). Ethanol concentration (from 30% to 60%) was not a significant factor for desorption ratio. Four kinds of anthocyanins were identified in black bean canning wastewater. The major anthocyanins were delphinidin 3-glucoside, petunidin 3glucoside and maldvidin 3-glucoside, with a small amount of petunidin 3, 5-diglucoside also present.展开更多
对以紫茄皮为原料提取的花青素进行大孔树脂纯化法纯化,探讨紫茄皮花青素的稳定性。通过静/动态吸附-解吸实验筛选大孔树脂、优化纯化工艺条件,在此基础上,探讨温度、p H、氧化/还原性物质、防腐剂、金属离子、糖等条件对紫茄皮中花青...对以紫茄皮为原料提取的花青素进行大孔树脂纯化法纯化,探讨紫茄皮花青素的稳定性。通过静/动态吸附-解吸实验筛选大孔树脂、优化纯化工艺条件,在此基础上,探讨温度、p H、氧化/还原性物质、防腐剂、金属离子、糖等条件对紫茄皮中花青素稳定性的影响。结果表明:在供试树脂中,AB-8大孔树脂为紫茄皮花青素纯化的最适吸附剂,其吸附最优条件为为吸附时间1 h,上样量260 m L,吸附流速1 m L/min;最佳解析条件为,解析时间1 h,洗脱液乙醇体积分数为40%,洗脱液体积为80 m L,吸附流速1 m L/min,纯化后的花青素纯度为95.54%;高温、p H、H2O2、抗坏血酸、苯甲酸钠、Fe3+等导致紫茄皮中花青素稳定性降低。大孔树脂纯化法适合于紫茄皮花青素的纯化。紫茄皮花青素应在4℃、p H1.0且避光条件下保存,在储藏过程中应避免接触铁容器,紫茄皮花青素对H2O2、苯甲酸钠很敏感,使用过程中应该避免二者存在。此外,紫茄皮花青素对糖稳定,生产中可依情况适量添加。展开更多
文摘Anthocyanins from black bean canning wastewater were isolated and purified on a laboratory scale by column chromatography, and then identified by high performance liquid chromatography electrospray tandem mass spectrometry. Dynamic adsorption and desorption were performed in glass columns packed with Sepabead Sp700 to optimize the purification process. Different temperatures during adsorption and desorption (25℃and 35℃) did not significantly affect the adsorption and desorption ratio. The adsorption ratio was significantly reduced when the flow rate increased from 1.5 mL/min to 2.5 mL/min. However, desorption ratio was not affected by flow rate (from 1.5 mL/min to 0.3 mL/min). Ethanol concentration (from 30% to 60%) was not a significant factor for desorption ratio. Four kinds of anthocyanins were identified in black bean canning wastewater. The major anthocyanins were delphinidin 3-glucoside, petunidin 3glucoside and maldvidin 3-glucoside, with a small amount of petunidin 3, 5-diglucoside also present.
文摘目的:建立AB-8大孔树脂纯化稠李花色苷的方法,并检测稠李花色苷体外抗氧化活性。方法:通过AB-8大孔树脂对稠李花色苷的动态吸附与解吸条件优化,确定最佳纯化条件;采用四唑氮蓝(nitroblue tetrazolium,NBT)光化还原法测定花色苷体外抗氧化活性,2’,7’-二氢二氯荧光素二乙酸酯(2’,7’-dichlorodihydrofluoresce in diacetate,DCFH-DA)法观察花色苷对H2O2诱导的N2A细胞氧化损伤的保护作用。结果:稠李花色苷的最佳动态纯化条件是:吸附流速0.01 m L/s,粗提液质量浓度8 mg/m L,过柱次数2次;解吸剂乙醇体积分数70%,流速0.01 m L/s,pH 3,纯化之后稠李花色苷的色价为57.1,纯化了16.31倍;NBT实验结果表明,稠李花色苷具有清除超氧阴离子自由基的能力,且具有较好的剂量-效应关系;0.025 mg/m L的稠李花色苷能够保护H2O2损伤的N2A细胞,0.05 mg/m L的稠李花色苷能够降低细胞内活性氧水平。结论:AB-8大孔树脂是纯化稠李花色苷的一种有效方法,稠李花色苷在一定质量浓度范围内具有较好的体外抗氧化活性。
文摘对以紫茄皮为原料提取的花青素进行大孔树脂纯化法纯化,探讨紫茄皮花青素的稳定性。通过静/动态吸附-解吸实验筛选大孔树脂、优化纯化工艺条件,在此基础上,探讨温度、p H、氧化/还原性物质、防腐剂、金属离子、糖等条件对紫茄皮中花青素稳定性的影响。结果表明:在供试树脂中,AB-8大孔树脂为紫茄皮花青素纯化的最适吸附剂,其吸附最优条件为为吸附时间1 h,上样量260 m L,吸附流速1 m L/min;最佳解析条件为,解析时间1 h,洗脱液乙醇体积分数为40%,洗脱液体积为80 m L,吸附流速1 m L/min,纯化后的花青素纯度为95.54%;高温、p H、H2O2、抗坏血酸、苯甲酸钠、Fe3+等导致紫茄皮中花青素稳定性降低。大孔树脂纯化法适合于紫茄皮花青素的纯化。紫茄皮花青素应在4℃、p H1.0且避光条件下保存,在储藏过程中应避免接触铁容器,紫茄皮花青素对H2O2、苯甲酸钠很敏感,使用过程中应该避免二者存在。此外,紫茄皮花青素对糖稳定,生产中可依情况适量添加。
