Aqueous zinc-ion batteries are promising due to inherent safety,low cost,low toxicity,and high volumetric capacity.However,issues of dendrites and side reactions between zinc metal anode and the electrolyte need to be...Aqueous zinc-ion batteries are promising due to inherent safety,low cost,low toxicity,and high volumetric capacity.However,issues of dendrites and side reactions between zinc metal anode and the electrolyte need to be solved for extended storage and cycle life.Here,we proposed that an electrolyte additive with an intermediate chelation strength of zinc ion—strong enough to exclude water molecules from the zinc metal-electrolyte interface and not too strong to cause a significant energy barrier for zinc ion dissociation—can benefit the electrochemical stability by suppressing hydrogen evolution reaction,overpotential growth,and den-drite formation.Penta-sodium diethylene-triaminepentaacetic acid salt was selected for such a purpose.It has a suitable chelating ability in aqueous solutions to adjust solvation sheath and can be readily polarized under electrical loading conditions to further improve the passivation.Zn||Zn symmetric cells can be stably operated over 3500 h at 1 mA cm^(-2).Zn||NH4V4O10 full cells with the additive show great cycling stability with 84.6%capacity retention after 500 cycles at 1 A g^(-1).Since the additive not only reduces H2 evolution and corrosion but also modifies Zn2+diffusion and deposition,highlyreversible Zn electrodes can be achieved as verified by the experimental results.Our work offers a practical approach to the logical design of reliable electrolytes for high-performance aqueous batteries.展开更多
This study aimed to characterize and identify calcium-chelating peptides from rabbit bone collagen and explore the underlying chelating mechanism.Collagen peptides and calcium were extracted from rabbit bone by instan...This study aimed to characterize and identify calcium-chelating peptides from rabbit bone collagen and explore the underlying chelating mechanism.Collagen peptides and calcium were extracted from rabbit bone by instant ejection steam explosion(ICSE)combined with enzymatic hydrolysis,followed by chelation reaction to prepare rabbit bone peptide-calcium chelate(RBCP-Ca).The chelating sites were further analyzed by liquid chromatography-tandem mass(LC-MS/MS)spectrometry while the chelating mechanism and binding modes were investigated.The structural characterization revealed that RBCP successfully chelated with calcium ions.Furthermore,LC-MS/MS analysis indicated that the binding sites included both acidic amino acids(Asp and Glu)and basic amino acids(Lys and Arg),Interestingly,three binding modes,namely Inter-Linking,Loop-Linking and Mono-Linking were for the first time found,while Inter-Linking mode accounted for the highest proportion(75.1%),suggesting that chelation of calcium ions frequently occurred between two peptides.Overall,this study provides a theoretical basis for the elucidation of chelation mechanism of calcium-chelating peptides.展开更多
The role of copper element has been an increasingly relevant topic in recent years in the fields of human and animal health, for both the study of new drugs and innovative food and feed supplements. This metal plays a...The role of copper element has been an increasingly relevant topic in recent years in the fields of human and animal health, for both the study of new drugs and innovative food and feed supplements. This metal plays an important role in the central nervous system, where it is associated with glutamatergic signaling, and it is widely involved in inflammatory processes. Thus, diseases involving copper(Ⅱ) dyshomeostasis often have neurological symptoms, as exemplified by Alzheimer's and other diseases(such as Parkinson's and Wilson's diseases). Moreover, imbalanced copper ion concentrations have also been associated with diabetes and certain types of cancer, including glioma. In this paper, we propose a comprehensive overview of recent results that show the importance of these metal ions in several pathologies, mainly Alzheimer's disease, through the lens of the development and use of copper chelators as research compounds and potential therapeutics if included in multi-target hybrid drugs. Seeing how copper homeostasis is important for the well-being of animals as well as humans, we shortly describe the state of the art regarding the effects of copper and its chelators in agriculture, livestock rearing, and aquaculture, as ingredients for the formulation of feed supplements as well as to prevent the effects of pollution on animal productions.展开更多
Early screening of diabetes retinopathy(DR)plays an important role in preventing irreversible blindness.Existing research has failed to fully explore effective DR lesion information in fundus maps.Besides,traditional ...Early screening of diabetes retinopathy(DR)plays an important role in preventing irreversible blindness.Existing research has failed to fully explore effective DR lesion information in fundus maps.Besides,traditional attention schemes have not considered the impact of lesion type differences on grading,resulting in unreasonable extraction of important lesion features.Therefore,this paper proposes a DR diagnosis scheme that integrates a multi-level patch attention generator(MPAG)and a lesion localization module(LLM).Firstly,MPAGis used to predict patches of different sizes and generate a weighted attention map based on the prediction score and the types of lesions contained in the patches,fully considering the impact of lesion type differences on grading,solving the problem that the attention maps of lesions cannot be further refined and then adapted to the final DR diagnosis task.Secondly,the LLM generates a global attention map based on localization.Finally,the weighted attention map and global attention map are weighted with the fundus map to fully explore effective DR lesion information and increase the attention of the classification network to lesion details.This paper demonstrates the effectiveness of the proposed method through extensive experiments on the public DDR dataset,obtaining an accuracy of 0.8064.展开更多
Structured illumination microscopy(SIM)is a popular and powerful super-resolution(SR)technique in biomedical research.However,the conventional reconstruction algorithm for SIM heavily relies on the accurate prior know...Structured illumination microscopy(SIM)is a popular and powerful super-resolution(SR)technique in biomedical research.However,the conventional reconstruction algorithm for SIM heavily relies on the accurate prior knowledge of illumination patterns and signal-to-noise ratio(SNR)of raw images.To obtain high-quality SR images,several raw images need to be captured under high fluorescence level,which further restricts SIM’s temporal resolution and its applications.Deep learning(DL)is a data-driven technology that has been used to expand the limits of optical microscopy.In this study,we propose a deep neural network based on multi-level wavelet and attention mechanism(MWAM)for SIM.Our results show that the MWAM network can extract high-frequency information contained in SIM raw images and accurately integrate it into the output image,resulting in superior SR images compared to those generated using wide-field images as input data.We also demonstrate that the number of SIM raw images can be reduced to three,with one image in each illumination orientation,to achieve the optimal tradeoff between temporal and spatial resolution.Furthermore,our MWAM network exhibits superior reconstruction ability on low-SNR images compared to conventional SIM algorithms.We have also analyzed the adaptability of this network on other biological samples and successfully applied the pretrained model to other SIM systems.展开更多
To observe the chelation of GRP78 with lead (Pb) and its localization changes, astroglial ceils from Wistar rat brain were primarily cultured in medium witb acetate Pb. The processes were terminated at different tim...To observe the chelation of GRP78 with lead (Pb) and its localization changes, astroglial ceils from Wistar rat brain were primarily cultured in medium witb acetate Pb. The processes were terminated at different time points. The immunoprecipitation (IP) and Western blotting were used for GRP78 purification and expression and the Pb concentration was determined by employing atomic absorption spectrophotometry (AAS). The localization change of GRP78 was observed with colloid gold immunoelectron microscopy. The results showed that the expression of GRP78 was increased significantly in the cells treated with 1.0 μmol/L acetate Pb for 24 h and peaked at 96-192 h (P〈0.01), and at the 12th day, the expression of GRP78 began to decrease but was still higher than normal (P〈0.05). Pb content started to increase when cells were treated by acetate Pb for 24 h, and the peak appeared at 8 day (P〈0.01), and then Pb content decreased gradually, but was still higher than normal (P〈0.05). GRP78 protein expression began to remarkably increase when it transferred from ER to the cytosol around the nuclei 24 h after treatment with Pb. It is concluded that GRP78 in astroglia could strongly chelate with Pb ions and it might be a target protein of Pb.展开更多
Iron is essential for all organisms including microbial,cancer and human cells. More than a quarter of the human population is affected by abnormalities of iron metabolism, mainly from iron deficiency and iron overloa...Iron is essential for all organisms including microbial,cancer and human cells. More than a quarter of the human population is affected by abnormalities of iron metabolism, mainly from iron deficiency and iron overload. Iron also plays an important role in free radical pathology and oxidative damage which is observed in almost all major diseases, cancer and ageing. New developments include the complete treatment of iron overload and reduction of morbidity and mortality in thalassaemia using deferiprone and selected deferiprone/deferoxamine combinations and also the use of the maltol iron complex in the treatment of iron deficiency anaemia. There is also a prospect of using deferiprone as a universal antioxidant in non iron overloaded diseases such as neurodegenerative, cardiovascular, renal, infectious diseases and cancer. New regulatory molecules of iron metabolism such as endogenous and dietary chelating molecules, hepcidin, mitochondrial ferritin and their role in health and disease is under evaluation. Similarly, new mechanisms of iron deposition, removal, distribution and toxicity have been identified using new techniques such as magnetic resonance imaging increasing our understanding of iron metabolic processes and the targeted treatment of related diseases. The uniform distribution of iron in iron overload between organs and within each organ is no longer valid. Several other controversies such as the toxicity impact of non transferrin bound iron vs injected iron, the excess levels of iron in tissues causing toxicity and the role of chelation on iron absorption need further investigation. Commercial interests of pharmaceutical companies and connections to leading journals are playing a crucial role in shaping worldwide medical opinion on drug sales and use but also patients' therapeutic outcome and safety. Major controversies include the selection criteria and risk/benefit assessment in the use of deferasirox in thalassaemia and more so in idiopathic haemochromatosis, thalassaemia intermedia and ex-thalassaemia transplanted patients who are safely treated with venesection. Iron chelating drugs can override normal regulatory pathways, correct iron imbalance and minimise iron toxicity. The use of iron chelating drugs as main, alternative or adjuvant therapy is in progress in many conditions, especially those with non established or effective therapies.展开更多
The sol-gel process of citric acid chelating with metal cations for the synthesis of normal spinel LiMn 2O 4 and the reaction mechanism were investigated by means of XRD,IR,TG-DTA, and SEM.The results show that at t...The sol-gel process of citric acid chelating with metal cations for the synthesis of normal spinel LiMn 2O 4 and the reaction mechanism were investigated by means of XRD,IR,TG-DTA, and SEM.The results show that at the beginning lithium citrate and chelate compound of citric acid with manganese ions formed,and then with heating the esterification and condensation reactions occured between them and glycol.The products obtained are polymers in which metal cations are distributed homogeneously on atomic scale that ensure high reactivity to cations of Li + and Mn 2+.Firing the gel prepared by this process,the lattice diffusions of solid reactant ions caused by non-homogeneity of reactants are eliminated and avoided.At 400℃ phase-pure LiMn 2O 4 with nanometer scale crystallization having precise stoichiometry and perfect crystallization can be obtained.The model of chelate coordinate of double-molecule between citric acid and Mn 2+ in the gel network is proposed.It is important for explaining the dispersion state of Mn 2+ and the formation process of gel by this model.展开更多
The influence of methionine supplementation on the efficacy of common antidotes to lead poisoning, calcium disodium ethylenediaminetetraacetate (CaNa_2EDTA) and D-penicillamine (DPA), was investigated in rats. The ani...The influence of methionine supplementation on the efficacy of common antidotes to lead poisoning, calcium disodium ethylenediaminetetraacetate (CaNa_2EDTA) and D-penicillamine (DPA), was investigated in rats. The animals were given lead acetate (0.1% in drinking water) for 12 weeks and thereafter treated with CaNa_2EDTA, DPA (0.3mmol/kg, intraperitoneally), DL-methionine (1.34 mmol/kg, intragastrically), or the combination of a chelating agent and methionine for 3 days. While chelating agents enhanced the urinary excretion of Pb, methionine increased the fecal excretion of Pb significantly. Treatment with the combination of a chelating agent and methionine did not potentiate the effect of each antidote. However, methionine supplementation increased the efficacy of both chelating agents in reducing the hepatic and renal Pb burden but not the blood Pb level. The Pb-induced inhibition of blood δ-aminolevulinic acid dehydratase activity and the increase in urinary excretion of δ-aminolevulinic acid were reversed to a certain extent by CaNa_2EDTA, DPA, and methionine but the combination did not improve their individual performances. The beneficial effects of methionine may be attributed to its ability to increase the bioavailability of glutathione (GSH), useful in chelating Pb and counteracting the toxic effects, as evidenced by restoration of the Pb-induced decrease in hepatic GSH level by treatment with methionine. Methionine may be useful as a supportive therapy in chelation of Pb. (c)1989 Academic Press. Inc.展开更多
The structure and electronic properties of a series of biologically active dithiolethiones (1) have been calculated using semi-empirical. Multi-linear regression analysis suggests that there is a reasonable correlat...The structure and electronic properties of a series of biologically active dithiolethiones (1) have been calculated using semi-empirical. Multi-linear regression analysis suggests that there is a reasonable correlation between the experimental activity of the derivatives against chelation activity and calculated properties such as the HOMO energies, molar refractivity, dipole moments and experimental partition coefficient. From the derived QSAR equations the 3-Methylthio-4p-Tolyle-1,2-Dithiolylium accompanying ion (CH3SO4) and 4-para-tolyl-1,2-dithiole-3-thione (2b and 2) are predicted to show the highest activity against chelation activity, while 3-Methylthio-5p-methoxy phenyl-1,2-Dithiolylium accompanying ion (I-) (3a) is predicted to be the least active in line with the experimental results.展开更多
Objective To synthesize some new a-mercapto-β-substituted aryl acrylic acids, characterize them and investigate their in vitro cadmium chelating ability. Methods Six α-mercapto-β-substituted aryl acrylic acids were...Objective To synthesize some new a-mercapto-β-substituted aryl acrylic acids, characterize them and investigate their in vitro cadmium chelating ability. Methods Six α-mercapto-β-substituted aryl acrylic acids were prepared by the alkaline hydrolysis of 5- (aryl methylene) rhodanines, obtained from the condensation of substituted aldehydes and rhodanine following the reported procedure. The new compounds were characterized by elemental analysis, infrared (IR) and nuclear magnetic resonance (NMR) spectroscopy. The liver and kidney from cadmium chloride pre-administered rats were homogenized and their nuclear mitochondrial fraction (NMF) and supernatant cytosol fraction (SCF) were separated. A measured volume of each fraction was dialyzed separately using 'dialysis sack' against buffered-KCl medium containing a compound in the final concentration of 1×10-3 mol/L for 3 h at 37℃. The whole content of 'sack' was subjected to cadmium estimation following digestion with cone. Nitric acid was detected using flame atomic absorption spectrometer. Results The in vitro screening showed that α-mercapto-β-(p-methoxyphenyl) acrylic acid (compound 2) andα-mercapto-β-(m-methoxy, p-hydroxyphenyl) acrylic acid (compound 4) were more effective than α-mercapto-β-thienyl acrylic acid (compound 1) and a-mercapto-β-(p-dimethylaminophenyl) acrylic acid (compound 3) in mobilizing cadmium as their dialyzable chelates. The presence of a methoxy group on the phenyl moiety (compounds 2 and 4) increases the metal chelating ability of mercapto acrylic acids. Conclusions Compounds 2 and 4 seem to have accessibility to the cellular system and capability of chelating-out the intracellularly bound cadmium.展开更多
[Objectives]This study aimed to optimize the chelation process for complex microelement iron supplement derived from pig blood by response surface methodology.[Methods]On the basis of single-factor test,p H value,conc...[Objectives]This study aimed to optimize the chelation process for complex microelement iron supplement derived from pig blood by response surface methodology.[Methods]On the basis of single-factor test,p H value,concentration of polypeptide solution and volume ratio of polypeptide solution to FeCl_2 solution were selected as influencing factors with Fe(II)chelation rate as the indicator for Box-Behnken central composite experimental design with three factors and three levels.The effects of three factors on the response value were analyzed by response surface methodology.[Results]The optimized chelation process for complex microelement iron supplement derived from pig blood by response surface methodology was as follows:pH 5.40,polypeptide solution concentration 2.27%,volume ratio of polypeptide solution to FeCl_2 solution 2.16∶1.Under this condition,the predictive Fe(II)chelation rate of iron supplement was 79.37%,while the actual value was 79.41%.[Conclusions]The optimized process may provide new thoughts for the development and utilization of complex microelement iron supplement derived from pig blood.展开更多
Traditional risk factors for cardiovascular disease can only assess risks for groups of people. New parameters of arterial stiffness are more reliable for predicting cardiovascular outcomes for individuals with and wi...Traditional risk factors for cardiovascular disease can only assess risks for groups of people. New parameters of arterial stiffness are more reliable for predicting cardiovascular outcomes for individuals with and without a cardiovascular history. The objective of this study was to assess the effects of Magnesium-EDTA chelation therapy using new methods and parameters such as pulse wave velocity (PWV), central blood pressure (SBPao) and endothelial function (Aix). We followed 43 patients with an abnormal PWV and SBPao, setting them up in two groups. The 21 patients in Group A had already been diagnosed with cardiovascular disease. The other 22 patients in Group B also showed abnormal PWV, SBPao and Aix, but showed no cardiovascular symptoms. Each patient in Groups A and B received one Mg-EDTA treatment per week. The total treatment plan consisted of 25 Mg-EDTA chelation treatments according to the standard protocol of IBCMT. After 25 Mg-EDTA chelation sessions, PWV and SBPao improved significantly in all patients of Groups A and B. In addition, Aix improved significantly in these patients, but remained abnormal. Group C included 18 asymptomatic patients with normal PWV or SBPao. Aix was abnormal in this group, but to a much lesser extent than Groups A and B. The 18 asymptomatic patients of Group C did not receive Mg-EDTA treatment. Observation showed no significant changes in all three parameters of arterial stiffness. The results of this study indicate that a course of treatment with Magnesium-EDTA chelation therapy significantly lowers cardiovascular risks. We conclude that Mg-EDTA chelation therapy improves PWV as an indicator of arterial stiffness, SBPao (central blood pressure) as an indicator of aortic elasticity and Aix (augmented aortic index) as an indicator of endothelial functioning. These improvements in PWV, SBPao and Aix demonstrate that atherosclerosis is a dynamic and (partially) reversible process.展开更多
The selective precipitation of zinc from zinc-nickel sulfate solution with the Zn/Ni molar ratio of20:1was studied.Dropwise addition of0.5mol/L NaOH solution into the zinc-nickel sulfate solution containing0,0.01,0.02...The selective precipitation of zinc from zinc-nickel sulfate solution with the Zn/Ni molar ratio of20:1was studied.Dropwise addition of0.5mol/L NaOH solution into the zinc-nickel sulfate solution containing0,0.01,0.02,0.03and0.04mol/L ethylene diamine tetraacetate(EDTA)as a chelating agent was done.The equilibrium analysis of precipitation pathway was performed using Visual MINTEQ program.The equilibrium analysis showed that the presence of small amounts of EDTA can prevent nickel precipitation in alkaline conditions without any negative effect on zinc precipitation.On this basis,more than90%of zinc could be precipitated as a product with about50%Zn and only0.11%Ni at pH=9.0merely as a result of the presence of0.03mol/L EDTA in the solution.The stirring time of120min after precipitation was found to be essential for more complete separation.The X-ray diffraction studies on the precipitate revealed that the precipitated phase was Zn4(OH)6SO4.4H2O.展开更多
Pipemidic acid is one of an efficient quinolone antibacterial drug.Thecomplexitybetween pipemidic acid“Hpipc”withgallium(Ⅲ),germanium(Ⅳ)and silicon(Ⅳ)afforded three molecular formulas of[Ga(pipc)2(H 2O)(Cl)]·...Pipemidic acid is one of an efficient quinolone antibacterial drug.Thecomplexitybetween pipemidic acid“Hpipc”withgallium(Ⅲ),germanium(Ⅳ)and silicon(Ⅳ)afforded three molecular formulas of[Ga(pipc)2(H 2O)(Cl)]·4H 2O,1,[Ge(pipc)2(Cl)2]·4H 2O,2 and[Si(pipc)2(Cl)2]·4H 2O,3 complexes.These three new complexes were characterized based on elemental analysis,conductance,FTIR,UV-Vis,^1HNMR and XRD spectroscopy.The pipc chelate exhibits O,O coordinated through the carbonyl(C O)and carboxylato(COO)of both oxygen atoms.Six coordinate geometry was proposed regarding complexes of 2 and 3,so the axial position was occupied by two coordinated chlorideatoms.In vitro,the antibacterial,antifungal,and anti-cancer assessments concerning the synthesized pipc complexes were scanned.These complexes have an excellent anti-microbial activity.展开更多
Lithium bis(trifluoromethanesulfonyl)imide(Li-TFSI)/4-tert-butylpyridine(tBP)is a classic doping system for the hole transport material Spiro-OMeTAD in typical n-i-p structure perovskite solar cells(PSCs),but this sys...Lithium bis(trifluoromethanesulfonyl)imide(Li-TFSI)/4-tert-butylpyridine(tBP)is a classic doping system for the hole transport material Spiro-OMeTAD in typical n-i-p structure perovskite solar cells(PSCs),but this system will cause many problems such as high hygroscopicity,Li+migration,pinholes and so on,which hinder PSC from maintaining high efficiency and stability for long-term.In this work,an effective strategy is demonstrated to improve the performance and stability of PSC by replacing t BP with 12-crown-4.The chelation of 12-crown-4 with Li+not only improves the doping effect of Li-TFSI,but also perfectly solves the problems caused by the Li-TFSI/tBP system.The PSC based on this strategy achieved a champion power conversion efficiency(PCE)over 21%,which is significantly better than the pristine device(19.37%).More importantly,the without encapsulated device based on Li-TFSI/12-crown-4 still maintains 87%of the initial PCE even after 60 days exposure in air,while the pristine device only maintains 22%of the initial PCE under the same aging conditions.This strategy paves a novel way for constructing efficient and stable PSCs.展开更多
Ethylene Diamine Tetra Acetic Acid (EDTA) chelation therapy has been considered a definitive alternative therapy for by-pass surgery in atherosclerotic cardiovascular disease for more than four decades. It is a relati...Ethylene Diamine Tetra Acetic Acid (EDTA) chelation therapy has been considered a definitive alternative therapy for by-pass surgery in atherosclerotic cardiovascular disease for more than four decades. It is a relatively inexpensive method believed to restore blood flow in atherosclerotic vessels. However, the benefits of chelation therapy yet remain controversial in the treatment of ischemic heart disease. We observed the effect of EDTA chelation therapy on exercise tolerance in 13 volunteering patients receiving conventional treatment for established symptomatic coronary heart disease. Each patient received 30 weekly infusions of EDTA followed by monthly 12 boosters according to the ACAM protocol (American College for Advancement in Medicine). This was in addition to the conventional therapies they received from their respective physician in hospital. Stress ECG, echocardiography and coronary angiogram findings were obtained at the beginning of treatment. The distance that a patient could walk on level ground at moderate speed and the number of steps he/she can climb up on a staircase until he/she begins to feel either chest pain or breathlessness were the two clinical parameters of exercise tolerance recorded to grade angina. Liver and renal functions were tested at 1st, 5th, 10th, 15th and 30th infusions. Of the 13 patients, 11 showed improvement in angina grading whilst 2 experienced no effect. One patient improved from angina grade IV to I, 6 from grade III to I, 1 from grade III to II and 3 from grade II to I. A statistically significant reduction in the mean score (p = 0.002) was noticed at 6th month of treatment when compared to that of the first month. A significant 1.7 fold increase (p = 0.009) in the mean SGPT level was observed at the 30th infusion when compared to the pre-treatment values. The SGOT level showed no significant change (p = 0.664). None of the patients showed clinical features of hepato-cellular damage. The mean serum creatinine level showed a trend for reduction (p = 0.083) with treatment. The recognized side effects of intravenous EDTA chelation therapy such as liver damage, renal damage, hypersensitivity, symptomatic hypocalcaemia, and thrombophlebitis were not encountered. Thus, EDTA chelation therapy as prescribed by the ACAM protocol seems safe and effective in improving exercise tolerance in ischemic heart disease when administered concurrently with conventional therapy.展开更多
With the construction of the power Internet of Things(IoT),communication between smart devices in urban distribution networks has been gradually moving towards high speed,high compatibility,and low latency,which provi...With the construction of the power Internet of Things(IoT),communication between smart devices in urban distribution networks has been gradually moving towards high speed,high compatibility,and low latency,which provides reliable support for reconfiguration optimization in urban distribution networks.Thus,this study proposed a deep reinforcement learning based multi-level dynamic reconfiguration method for urban distribution networks in a cloud-edge collaboration architecture to obtain a real-time optimal multi-level dynamic reconfiguration solution.First,the multi-level dynamic reconfiguration method was discussed,which included feeder-,transformer-,and substation-levels.Subsequently,the multi-agent system was combined with the cloud-edge collaboration architecture to build a deep reinforcement learning model for multi-level dynamic reconfiguration in an urban distribution network.The cloud-edge collaboration architecture can effectively support the multi-agent system to conduct“centralized training and decentralized execution”operation modes and improve the learning efficiency of the model.Thereafter,for a multi-agent system,this study adopted a combination of offline and online learning to endow the model with the ability to realize automatic optimization and updation of the strategy.In the offline learning phase,a Q-learning-based multi-agent conservative Q-learning(MACQL)algorithm was proposed to stabilize the learning results and reduce the risk of the next online learning phase.In the online learning phase,a multi-agent deep deterministic policy gradient(MADDPG)algorithm based on policy gradients was proposed to explore the action space and update the experience pool.Finally,the effectiveness of the proposed method was verified through a simulation analysis of a real-world 445-node system.展开更多
An 87-year-old woman with primary open-angle glaucoma presented to our hospital. Although the combined cataract and minimally invasive glaucoma surgeries (MIGS) were an appropriate surgical option, the presence of ban...An 87-year-old woman with primary open-angle glaucoma presented to our hospital. Although the combined cataract and minimally invasive glaucoma surgeries (MIGS) were an appropriate surgical option, the presence of band keratopathy made it difficult to perform ab interno glaucoma surgery in her right eye (OD);therefore, the corneal opacity was removed using ethylenediaminetetraacetic acid (EDTA) chelation procedure. One month after chelation, microhook ab interno trabeculotomy and cataract surgery were performed successfully. Clear intraoperative visualization of the angle structures is critical for the success of these MIGS procedures. In glaucomatous eyes that require MIGS, EDTA chelation is a good neoadjuvant therapy for coexisting band keratopathy.展开更多
The complexation of BDHTD (4-benzylidenamino-4,5-dihydro-lH-l,2,4-triazol-5-one derivatives) by divalent doubly charged metal ions M2+ (M = Mg, Ca, Fe and Cu) has been investigated using the density functional me...The complexation of BDHTD (4-benzylidenamino-4,5-dihydro-lH-l,2,4-triazol-5-one derivatives) by divalent doubly charged metal ions M2+ (M = Mg, Ca, Fe and Cu) has been investigated using the density functional method B3LYP. Two distinct coordination modes (k2-O,O and k2-O,N) have been taken into account. Geometry optimizations have been performed in gas-phase and solution-phase: acetonitrile and DMF (N,N-dimethylformamide) with the basis set 6-31G(d,p). The B3LYP method was also used to calculate the stability and free energies of the 24 complexes of BDHTD with metal ions M2+ (M = Mg, Ca, Fe and Cu) respectively in gas-phase and solution-phase: acetonitrile and DMF. Results indicate that k2-O,N structures are most stable in gas-phase. The influence of substitution on the stability is sensitive in solution-phase. The interaction energies of complexation process in various media have been calculated at B3LYP/6-31G(d,p) and CCSD(T) level. The MIA (metal ion affinity) of BDHTD with M2+ (M = Mg, Ca, Fe and Cu) in various media has been explored. The results show that the M1A highly varies with the coordination mode and substitution effect. From the calculated Gibb energies of complexation in various media, it is revealed that the complexation is possible in gas in acetonitrile. The ligand's affinity toward individual cation M2+ (M = Mg, Ca, Fe and Cu) has been analysed. A significant reduce of BDEs observed confirms the decrease of the antioxidant activity by the metal chelation. The charge transfer induced by metal chelation is examined using the NBO analysis.展开更多
基金This work is financially supported by National Natural Science Foundation of China(NSFC-No.52173257 and 52372064).
文摘Aqueous zinc-ion batteries are promising due to inherent safety,low cost,low toxicity,and high volumetric capacity.However,issues of dendrites and side reactions between zinc metal anode and the electrolyte need to be solved for extended storage and cycle life.Here,we proposed that an electrolyte additive with an intermediate chelation strength of zinc ion—strong enough to exclude water molecules from the zinc metal-electrolyte interface and not too strong to cause a significant energy barrier for zinc ion dissociation—can benefit the electrochemical stability by suppressing hydrogen evolution reaction,overpotential growth,and den-drite formation.Penta-sodium diethylene-triaminepentaacetic acid salt was selected for such a purpose.It has a suitable chelating ability in aqueous solutions to adjust solvation sheath and can be readily polarized under electrical loading conditions to further improve the passivation.Zn||Zn symmetric cells can be stably operated over 3500 h at 1 mA cm^(-2).Zn||NH4V4O10 full cells with the additive show great cycling stability with 84.6%capacity retention after 500 cycles at 1 A g^(-1).Since the additive not only reduces H2 evolution and corrosion but also modifies Zn2+diffusion and deposition,highlyreversible Zn electrodes can be achieved as verified by the experimental results.Our work offers a practical approach to the logical design of reliable electrolytes for high-performance aqueous batteries.
基金granted by the National Key R&D Program of China (2021YFD21001005)National Natural Science Foundation of China (31972102,32101980)+1 种基金Special key project of Chongqing technology innovation and application development (cstc2021jscx-cylhX0014)Chongqing Technology Innovation and Application Development Special Project (cstc2021jscx-tpyzxX0014)。
文摘This study aimed to characterize and identify calcium-chelating peptides from rabbit bone collagen and explore the underlying chelating mechanism.Collagen peptides and calcium were extracted from rabbit bone by instant ejection steam explosion(ICSE)combined with enzymatic hydrolysis,followed by chelation reaction to prepare rabbit bone peptide-calcium chelate(RBCP-Ca).The chelating sites were further analyzed by liquid chromatography-tandem mass(LC-MS/MS)spectrometry while the chelating mechanism and binding modes were investigated.The structural characterization revealed that RBCP successfully chelated with calcium ions.Furthermore,LC-MS/MS analysis indicated that the binding sites included both acidic amino acids(Asp and Glu)and basic amino acids(Lys and Arg),Interestingly,three binding modes,namely Inter-Linking,Loop-Linking and Mono-Linking were for the first time found,while Inter-Linking mode accounted for the highest proportion(75.1%),suggesting that chelation of calcium ions frequently occurred between two peptides.Overall,this study provides a theoretical basis for the elucidation of chelation mechanism of calcium-chelating peptides.
文摘The role of copper element has been an increasingly relevant topic in recent years in the fields of human and animal health, for both the study of new drugs and innovative food and feed supplements. This metal plays an important role in the central nervous system, where it is associated with glutamatergic signaling, and it is widely involved in inflammatory processes. Thus, diseases involving copper(Ⅱ) dyshomeostasis often have neurological symptoms, as exemplified by Alzheimer's and other diseases(such as Parkinson's and Wilson's diseases). Moreover, imbalanced copper ion concentrations have also been associated with diabetes and certain types of cancer, including glioma. In this paper, we propose a comprehensive overview of recent results that show the importance of these metal ions in several pathologies, mainly Alzheimer's disease, through the lens of the development and use of copper chelators as research compounds and potential therapeutics if included in multi-target hybrid drugs. Seeing how copper homeostasis is important for the well-being of animals as well as humans, we shortly describe the state of the art regarding the effects of copper and its chelators in agriculture, livestock rearing, and aquaculture, as ingredients for the formulation of feed supplements as well as to prevent the effects of pollution on animal productions.
基金supported in part by the Research on the Application of Multimodal Artificial Intelligence in Diagnosis and Treatment of Type 2 Diabetes under Grant No.2020SK50910in part by the Hunan Provincial Natural Science Foundation of China under Grant 2023JJ60020.
文摘Early screening of diabetes retinopathy(DR)plays an important role in preventing irreversible blindness.Existing research has failed to fully explore effective DR lesion information in fundus maps.Besides,traditional attention schemes have not considered the impact of lesion type differences on grading,resulting in unreasonable extraction of important lesion features.Therefore,this paper proposes a DR diagnosis scheme that integrates a multi-level patch attention generator(MPAG)and a lesion localization module(LLM).Firstly,MPAGis used to predict patches of different sizes and generate a weighted attention map based on the prediction score and the types of lesions contained in the patches,fully considering the impact of lesion type differences on grading,solving the problem that the attention maps of lesions cannot be further refined and then adapted to the final DR diagnosis task.Secondly,the LLM generates a global attention map based on localization.Finally,the weighted attention map and global attention map are weighted with the fundus map to fully explore effective DR lesion information and increase the attention of the classification network to lesion details.This paper demonstrates the effectiveness of the proposed method through extensive experiments on the public DDR dataset,obtaining an accuracy of 0.8064.
基金supported by the National Natural Science Foundation of China(Grant Nos.62005307 and 61975228).
文摘Structured illumination microscopy(SIM)is a popular and powerful super-resolution(SR)technique in biomedical research.However,the conventional reconstruction algorithm for SIM heavily relies on the accurate prior knowledge of illumination patterns and signal-to-noise ratio(SNR)of raw images.To obtain high-quality SR images,several raw images need to be captured under high fluorescence level,which further restricts SIM’s temporal resolution and its applications.Deep learning(DL)is a data-driven technology that has been used to expand the limits of optical microscopy.In this study,we propose a deep neural network based on multi-level wavelet and attention mechanism(MWAM)for SIM.Our results show that the MWAM network can extract high-frequency information contained in SIM raw images and accurately integrate it into the output image,resulting in superior SR images compared to those generated using wide-field images as input data.We also demonstrate that the number of SIM raw images can be reduced to three,with one image in each illumination orientation,to achieve the optimal tradeoff between temporal and spatial resolution.Furthermore,our MWAM network exhibits superior reconstruction ability on low-SNR images compared to conventional SIM algorithms.We have also analyzed the adaptability of this network on other biological samples and successfully applied the pretrained model to other SIM systems.
基金supported by a grant from the National Natural Sciences Foundation of China(No.39970651)
文摘To observe the chelation of GRP78 with lead (Pb) and its localization changes, astroglial ceils from Wistar rat brain were primarily cultured in medium witb acetate Pb. The processes were terminated at different time points. The immunoprecipitation (IP) and Western blotting were used for GRP78 purification and expression and the Pb concentration was determined by employing atomic absorption spectrophotometry (AAS). The localization change of GRP78 was observed with colloid gold immunoelectron microscopy. The results showed that the expression of GRP78 was increased significantly in the cells treated with 1.0 μmol/L acetate Pb for 24 h and peaked at 96-192 h (P〈0.01), and at the 12th day, the expression of GRP78 began to decrease but was still higher than normal (P〈0.05). Pb content started to increase when cells were treated by acetate Pb for 24 h, and the peak appeared at 8 day (P〈0.01), and then Pb content decreased gradually, but was still higher than normal (P〈0.05). GRP78 protein expression began to remarkably increase when it transferred from ER to the cytosol around the nuclei 24 h after treatment with Pb. It is concluded that GRP78 in astroglia could strongly chelate with Pb ions and it might be a target protein of Pb.
文摘Iron is essential for all organisms including microbial,cancer and human cells. More than a quarter of the human population is affected by abnormalities of iron metabolism, mainly from iron deficiency and iron overload. Iron also plays an important role in free radical pathology and oxidative damage which is observed in almost all major diseases, cancer and ageing. New developments include the complete treatment of iron overload and reduction of morbidity and mortality in thalassaemia using deferiprone and selected deferiprone/deferoxamine combinations and also the use of the maltol iron complex in the treatment of iron deficiency anaemia. There is also a prospect of using deferiprone as a universal antioxidant in non iron overloaded diseases such as neurodegenerative, cardiovascular, renal, infectious diseases and cancer. New regulatory molecules of iron metabolism such as endogenous and dietary chelating molecules, hepcidin, mitochondrial ferritin and their role in health and disease is under evaluation. Similarly, new mechanisms of iron deposition, removal, distribution and toxicity have been identified using new techniques such as magnetic resonance imaging increasing our understanding of iron metabolic processes and the targeted treatment of related diseases. The uniform distribution of iron in iron overload between organs and within each organ is no longer valid. Several other controversies such as the toxicity impact of non transferrin bound iron vs injected iron, the excess levels of iron in tissues causing toxicity and the role of chelation on iron absorption need further investigation. Commercial interests of pharmaceutical companies and connections to leading journals are playing a crucial role in shaping worldwide medical opinion on drug sales and use but also patients' therapeutic outcome and safety. Major controversies include the selection criteria and risk/benefit assessment in the use of deferasirox in thalassaemia and more so in idiopathic haemochromatosis, thalassaemia intermedia and ex-thalassaemia transplanted patients who are safely treated with venesection. Iron chelating drugs can override normal regulatory pathways, correct iron imbalance and minimise iron toxicity. The use of iron chelating drugs as main, alternative or adjuvant therapy is in progress in many conditions, especially those with non established or effective therapies.
基金theNationalNaturalScienceFoundationofChina (No .5 9972 0 2 7)theNaturalScienceFoundationofHubeiProvince (No .2 0 0 2AB0 74) )
文摘The sol-gel process of citric acid chelating with metal cations for the synthesis of normal spinel LiMn 2O 4 and the reaction mechanism were investigated by means of XRD,IR,TG-DTA, and SEM.The results show that at the beginning lithium citrate and chelate compound of citric acid with manganese ions formed,and then with heating the esterification and condensation reactions occured between them and glycol.The products obtained are polymers in which metal cations are distributed homogeneously on atomic scale that ensure high reactivity to cations of Li + and Mn 2+.Firing the gel prepared by this process,the lattice diffusions of solid reactant ions caused by non-homogeneity of reactants are eliminated and avoided.At 400℃ phase-pure LiMn 2O 4 with nanometer scale crystallization having precise stoichiometry and perfect crystallization can be obtained.The model of chelate coordinate of double-molecule between citric acid and Mn 2+ in the gel network is proposed.It is important for explaining the dispersion state of Mn 2+ and the formation process of gel by this model.
文摘The influence of methionine supplementation on the efficacy of common antidotes to lead poisoning, calcium disodium ethylenediaminetetraacetate (CaNa_2EDTA) and D-penicillamine (DPA), was investigated in rats. The animals were given lead acetate (0.1% in drinking water) for 12 weeks and thereafter treated with CaNa_2EDTA, DPA (0.3mmol/kg, intraperitoneally), DL-methionine (1.34 mmol/kg, intragastrically), or the combination of a chelating agent and methionine for 3 days. While chelating agents enhanced the urinary excretion of Pb, methionine increased the fecal excretion of Pb significantly. Treatment with the combination of a chelating agent and methionine did not potentiate the effect of each antidote. However, methionine supplementation increased the efficacy of both chelating agents in reducing the hepatic and renal Pb burden but not the blood Pb level. The Pb-induced inhibition of blood δ-aminolevulinic acid dehydratase activity and the increase in urinary excretion of δ-aminolevulinic acid were reversed to a certain extent by CaNa_2EDTA, DPA, and methionine but the combination did not improve their individual performances. The beneficial effects of methionine may be attributed to its ability to increase the bioavailability of glutathione (GSH), useful in chelating Pb and counteracting the toxic effects, as evidenced by restoration of the Pb-induced decrease in hepatic GSH level by treatment with methionine. Methionine may be useful as a supportive therapy in chelation of Pb. (c)1989 Academic Press. Inc.
文摘The structure and electronic properties of a series of biologically active dithiolethiones (1) have been calculated using semi-empirical. Multi-linear regression analysis suggests that there is a reasonable correlation between the experimental activity of the derivatives against chelation activity and calculated properties such as the HOMO energies, molar refractivity, dipole moments and experimental partition coefficient. From the derived QSAR equations the 3-Methylthio-4p-Tolyle-1,2-Dithiolylium accompanying ion (CH3SO4) and 4-para-tolyl-1,2-dithiole-3-thione (2b and 2) are predicted to show the highest activity against chelation activity, while 3-Methylthio-5p-methoxy phenyl-1,2-Dithiolylium accompanying ion (I-) (3a) is predicted to be the least active in line with the experimental results.
基金This research was supported by the Council of Science and Technology, U. P state government.
文摘Objective To synthesize some new a-mercapto-β-substituted aryl acrylic acids, characterize them and investigate their in vitro cadmium chelating ability. Methods Six α-mercapto-β-substituted aryl acrylic acids were prepared by the alkaline hydrolysis of 5- (aryl methylene) rhodanines, obtained from the condensation of substituted aldehydes and rhodanine following the reported procedure. The new compounds were characterized by elemental analysis, infrared (IR) and nuclear magnetic resonance (NMR) spectroscopy. The liver and kidney from cadmium chloride pre-administered rats were homogenized and their nuclear mitochondrial fraction (NMF) and supernatant cytosol fraction (SCF) were separated. A measured volume of each fraction was dialyzed separately using 'dialysis sack' against buffered-KCl medium containing a compound in the final concentration of 1×10-3 mol/L for 3 h at 37℃. The whole content of 'sack' was subjected to cadmium estimation following digestion with cone. Nitric acid was detected using flame atomic absorption spectrometer. Results The in vitro screening showed that α-mercapto-β-(p-methoxyphenyl) acrylic acid (compound 2) andα-mercapto-β-(m-methoxy, p-hydroxyphenyl) acrylic acid (compound 4) were more effective than α-mercapto-β-thienyl acrylic acid (compound 1) and a-mercapto-β-(p-dimethylaminophenyl) acrylic acid (compound 3) in mobilizing cadmium as their dialyzable chelates. The presence of a methoxy group on the phenyl moiety (compounds 2 and 4) increases the metal chelating ability of mercapto acrylic acids. Conclusions Compounds 2 and 4 seem to have accessibility to the cellular system and capability of chelating-out the intracellularly bound cadmium.
基金Supported by Youth Fund of National Natural Science Foundation of China(31801673)Talent Development Fund of Anhui Academy of Agricultural Sciences(17F1205)+2 种基金Youth Innovation Fund of President of Anhui Academy of Agricultural Sciences(17B1220)Team Building Project of Anhui Academy of Agricultural Sciences(18C1225)Youth Fund of Natural Science Foundation of Anhui Province(1808085QC94)
文摘[Objectives]This study aimed to optimize the chelation process for complex microelement iron supplement derived from pig blood by response surface methodology.[Methods]On the basis of single-factor test,p H value,concentration of polypeptide solution and volume ratio of polypeptide solution to FeCl_2 solution were selected as influencing factors with Fe(II)chelation rate as the indicator for Box-Behnken central composite experimental design with three factors and three levels.The effects of three factors on the response value were analyzed by response surface methodology.[Results]The optimized chelation process for complex microelement iron supplement derived from pig blood by response surface methodology was as follows:pH 5.40,polypeptide solution concentration 2.27%,volume ratio of polypeptide solution to FeCl_2 solution 2.16∶1.Under this condition,the predictive Fe(II)chelation rate of iron supplement was 79.37%,while the actual value was 79.41%.[Conclusions]The optimized process may provide new thoughts for the development and utilization of complex microelement iron supplement derived from pig blood.
文摘Traditional risk factors for cardiovascular disease can only assess risks for groups of people. New parameters of arterial stiffness are more reliable for predicting cardiovascular outcomes for individuals with and without a cardiovascular history. The objective of this study was to assess the effects of Magnesium-EDTA chelation therapy using new methods and parameters such as pulse wave velocity (PWV), central blood pressure (SBPao) and endothelial function (Aix). We followed 43 patients with an abnormal PWV and SBPao, setting them up in two groups. The 21 patients in Group A had already been diagnosed with cardiovascular disease. The other 22 patients in Group B also showed abnormal PWV, SBPao and Aix, but showed no cardiovascular symptoms. Each patient in Groups A and B received one Mg-EDTA treatment per week. The total treatment plan consisted of 25 Mg-EDTA chelation treatments according to the standard protocol of IBCMT. After 25 Mg-EDTA chelation sessions, PWV and SBPao improved significantly in all patients of Groups A and B. In addition, Aix improved significantly in these patients, but remained abnormal. Group C included 18 asymptomatic patients with normal PWV or SBPao. Aix was abnormal in this group, but to a much lesser extent than Groups A and B. The 18 asymptomatic patients of Group C did not receive Mg-EDTA treatment. Observation showed no significant changes in all three parameters of arterial stiffness. The results of this study indicate that a course of treatment with Magnesium-EDTA chelation therapy significantly lowers cardiovascular risks. We conclude that Mg-EDTA chelation therapy improves PWV as an indicator of arterial stiffness, SBPao (central blood pressure) as an indicator of aortic elasticity and Aix (augmented aortic index) as an indicator of endothelial functioning. These improvements in PWV, SBPao and Aix demonstrate that atherosclerosis is a dynamic and (partially) reversible process.
文摘The selective precipitation of zinc from zinc-nickel sulfate solution with the Zn/Ni molar ratio of20:1was studied.Dropwise addition of0.5mol/L NaOH solution into the zinc-nickel sulfate solution containing0,0.01,0.02,0.03and0.04mol/L ethylene diamine tetraacetate(EDTA)as a chelating agent was done.The equilibrium analysis of precipitation pathway was performed using Visual MINTEQ program.The equilibrium analysis showed that the presence of small amounts of EDTA can prevent nickel precipitation in alkaline conditions without any negative effect on zinc precipitation.On this basis,more than90%of zinc could be precipitated as a product with about50%Zn and only0.11%Ni at pH=9.0merely as a result of the presence of0.03mol/L EDTA in the solution.The stirring time of120min after precipitation was found to be essential for more complete separation.The X-ray diffraction studies on the precipitate revealed that the precipitated phase was Zn4(OH)6SO4.4H2O.
基金funded by the Deanship of Scientific Research at Princess Nourah bint Abdulrahman University through the Fast-track Research Funding Program。
文摘Pipemidic acid is one of an efficient quinolone antibacterial drug.Thecomplexitybetween pipemidic acid“Hpipc”withgallium(Ⅲ),germanium(Ⅳ)and silicon(Ⅳ)afforded three molecular formulas of[Ga(pipc)2(H 2O)(Cl)]·4H 2O,1,[Ge(pipc)2(Cl)2]·4H 2O,2 and[Si(pipc)2(Cl)2]·4H 2O,3 complexes.These three new complexes were characterized based on elemental analysis,conductance,FTIR,UV-Vis,^1HNMR and XRD spectroscopy.The pipc chelate exhibits O,O coordinated through the carbonyl(C O)and carboxylato(COO)of both oxygen atoms.Six coordinate geometry was proposed regarding complexes of 2 and 3,so the axial position was occupied by two coordinated chlorideatoms.In vitro,the antibacterial,antifungal,and anti-cancer assessments concerning the synthesized pipc complexes were scanned.These complexes have an excellent anti-microbial activity.
基金the National Natural Science Foundation of China(22175029 and 62104031)the Sichuan Science and Technology Program(2019YJ0162)+3 种基金the Open Foundation of State Key Laboratory of Electronic Thin Films and Integrated Devices(KFJJ202109)the Natural Science Foundation of Shenzhen Innovation Committee(JCYJ20210324135614040)the Technical Field Funds of 173 Project(2021-JCJQ-JJ-0663)the Fundamental Research Funds for the Central Universities of China(ZYGX2021J010 and Y030202059018023)for financial support。
文摘Lithium bis(trifluoromethanesulfonyl)imide(Li-TFSI)/4-tert-butylpyridine(tBP)is a classic doping system for the hole transport material Spiro-OMeTAD in typical n-i-p structure perovskite solar cells(PSCs),but this system will cause many problems such as high hygroscopicity,Li+migration,pinholes and so on,which hinder PSC from maintaining high efficiency and stability for long-term.In this work,an effective strategy is demonstrated to improve the performance and stability of PSC by replacing t BP with 12-crown-4.The chelation of 12-crown-4 with Li+not only improves the doping effect of Li-TFSI,but also perfectly solves the problems caused by the Li-TFSI/tBP system.The PSC based on this strategy achieved a champion power conversion efficiency(PCE)over 21%,which is significantly better than the pristine device(19.37%).More importantly,the without encapsulated device based on Li-TFSI/12-crown-4 still maintains 87%of the initial PCE even after 60 days exposure in air,while the pristine device only maintains 22%of the initial PCE under the same aging conditions.This strategy paves a novel way for constructing efficient and stable PSCs.
文摘Ethylene Diamine Tetra Acetic Acid (EDTA) chelation therapy has been considered a definitive alternative therapy for by-pass surgery in atherosclerotic cardiovascular disease for more than four decades. It is a relatively inexpensive method believed to restore blood flow in atherosclerotic vessels. However, the benefits of chelation therapy yet remain controversial in the treatment of ischemic heart disease. We observed the effect of EDTA chelation therapy on exercise tolerance in 13 volunteering patients receiving conventional treatment for established symptomatic coronary heart disease. Each patient received 30 weekly infusions of EDTA followed by monthly 12 boosters according to the ACAM protocol (American College for Advancement in Medicine). This was in addition to the conventional therapies they received from their respective physician in hospital. Stress ECG, echocardiography and coronary angiogram findings were obtained at the beginning of treatment. The distance that a patient could walk on level ground at moderate speed and the number of steps he/she can climb up on a staircase until he/she begins to feel either chest pain or breathlessness were the two clinical parameters of exercise tolerance recorded to grade angina. Liver and renal functions were tested at 1st, 5th, 10th, 15th and 30th infusions. Of the 13 patients, 11 showed improvement in angina grading whilst 2 experienced no effect. One patient improved from angina grade IV to I, 6 from grade III to I, 1 from grade III to II and 3 from grade II to I. A statistically significant reduction in the mean score (p = 0.002) was noticed at 6th month of treatment when compared to that of the first month. A significant 1.7 fold increase (p = 0.009) in the mean SGPT level was observed at the 30th infusion when compared to the pre-treatment values. The SGOT level showed no significant change (p = 0.664). None of the patients showed clinical features of hepato-cellular damage. The mean serum creatinine level showed a trend for reduction (p = 0.083) with treatment. The recognized side effects of intravenous EDTA chelation therapy such as liver damage, renal damage, hypersensitivity, symptomatic hypocalcaemia, and thrombophlebitis were not encountered. Thus, EDTA chelation therapy as prescribed by the ACAM protocol seems safe and effective in improving exercise tolerance in ischemic heart disease when administered concurrently with conventional therapy.
基金supported by the National Natural Science Foundation of China under Grant 52077146.
文摘With the construction of the power Internet of Things(IoT),communication between smart devices in urban distribution networks has been gradually moving towards high speed,high compatibility,and low latency,which provides reliable support for reconfiguration optimization in urban distribution networks.Thus,this study proposed a deep reinforcement learning based multi-level dynamic reconfiguration method for urban distribution networks in a cloud-edge collaboration architecture to obtain a real-time optimal multi-level dynamic reconfiguration solution.First,the multi-level dynamic reconfiguration method was discussed,which included feeder-,transformer-,and substation-levels.Subsequently,the multi-agent system was combined with the cloud-edge collaboration architecture to build a deep reinforcement learning model for multi-level dynamic reconfiguration in an urban distribution network.The cloud-edge collaboration architecture can effectively support the multi-agent system to conduct“centralized training and decentralized execution”operation modes and improve the learning efficiency of the model.Thereafter,for a multi-agent system,this study adopted a combination of offline and online learning to endow the model with the ability to realize automatic optimization and updation of the strategy.In the offline learning phase,a Q-learning-based multi-agent conservative Q-learning(MACQL)algorithm was proposed to stabilize the learning results and reduce the risk of the next online learning phase.In the online learning phase,a multi-agent deep deterministic policy gradient(MADDPG)algorithm based on policy gradients was proposed to explore the action space and update the experience pool.Finally,the effectiveness of the proposed method was verified through a simulation analysis of a real-world 445-node system.
文摘An 87-year-old woman with primary open-angle glaucoma presented to our hospital. Although the combined cataract and minimally invasive glaucoma surgeries (MIGS) were an appropriate surgical option, the presence of band keratopathy made it difficult to perform ab interno glaucoma surgery in her right eye (OD);therefore, the corneal opacity was removed using ethylenediaminetetraacetic acid (EDTA) chelation procedure. One month after chelation, microhook ab interno trabeculotomy and cataract surgery were performed successfully. Clear intraoperative visualization of the angle structures is critical for the success of these MIGS procedures. In glaucomatous eyes that require MIGS, EDTA chelation is a good neoadjuvant therapy for coexisting band keratopathy.
文摘The complexation of BDHTD (4-benzylidenamino-4,5-dihydro-lH-l,2,4-triazol-5-one derivatives) by divalent doubly charged metal ions M2+ (M = Mg, Ca, Fe and Cu) has been investigated using the density functional method B3LYP. Two distinct coordination modes (k2-O,O and k2-O,N) have been taken into account. Geometry optimizations have been performed in gas-phase and solution-phase: acetonitrile and DMF (N,N-dimethylformamide) with the basis set 6-31G(d,p). The B3LYP method was also used to calculate the stability and free energies of the 24 complexes of BDHTD with metal ions M2+ (M = Mg, Ca, Fe and Cu) respectively in gas-phase and solution-phase: acetonitrile and DMF. Results indicate that k2-O,N structures are most stable in gas-phase. The influence of substitution on the stability is sensitive in solution-phase. The interaction energies of complexation process in various media have been calculated at B3LYP/6-31G(d,p) and CCSD(T) level. The MIA (metal ion affinity) of BDHTD with M2+ (M = Mg, Ca, Fe and Cu) in various media has been explored. The results show that the M1A highly varies with the coordination mode and substitution effect. From the calculated Gibb energies of complexation in various media, it is revealed that the complexation is possible in gas in acetonitrile. The ligand's affinity toward individual cation M2+ (M = Mg, Ca, Fe and Cu) has been analysed. A significant reduce of BDEs observed confirms the decrease of the antioxidant activity by the metal chelation. The charge transfer induced by metal chelation is examined using the NBO analysis.