Frequency-modulated continuous-wave multiplexed gas sensingbased on optical frequency comb calibration

Laser absorption spectroscopy has proven to be an effective approach for gas sensing, which plays an important rolein the fields of military, industry, medicine and basic research. This paper presents a multiplexed gas sensing system basedon optical frequency comb (OFC) calibrated frequency-modulated continuous-wave (FMCW) tuning nonlinearity. Thesystem can be used for multi-parameter synchronous measurement of gas absorption spectrum and multiplexed opticalpath. Multi-channel parallel detection is realized by combining wavelength division multiplexing (WDM) and frequencydivision multiplexing (FDM) techniques. By introducing nonlinear optical crystals, broadband spectrum detection is simultaneouslyachieved over a bandwidth of hundreds of nanometers. An OFC with ultra-high frequency stability is used asthe frequency calibration source, which guarantees the measurement accuracy. The test samples involve H13C14N, C_(2)H_(2)and Rb vapor cells of varying densities and 5 parallel measurement experiments are designed. The results show that themeasurement accuracies of spectral absorption line and the optical path are 150 MHz and 20 m, respectively. The schemeoffers the advantages of multiplexed, multi-parameter, wide spectrum and high resolution detection, which can realize theidentification of multi-gas components and the high-precision inversion of absorption lines under different environments.The proposed sensor demonstrates great potential in the field of high-resolution absorption spectrum measurement for gassensing applications.

Semi-implantable device based on multiplexed microfilament electrode cluster for continuous monitoring of physiological ions

Modern medicine is increasingly interested in advanced sensors to detect and analyze biochemical indicators.Ion sensors based on potentiometric methods are a promising platform for monitoring physiological ions in biological subjects.Current semi-implantable devices are mainly based on single-parameter detection.Miniaturized semi-implantable electrodes for multiparameter sensing have more restrictions on the electrode size due to biocompatibility considerations,but reducing the electrode surface area could potentially limit electrode sensitivity.This study developed a semi-implantable device system comprising a multiplexed microfilament electrode cluster(MMEC)and a printed circuit board for real-time monitoring of intra-tissue K^(+),Ca^(2+),and Na^(+)concentrations.The electrode surface area was less important for the potentiometric sensing mechanism,suggesting the feasibility of using a tiny fiber-like electrode for potentiometric sensing.The MMEC device exhibited a broad linear response(K^(+):2–32 mmol/L;Ca^(2+):0.5–4 mmol/L;Na^(+):10–160 mmol/L),high sensitivity(about 20–45 mV/decade),temporal stability(>2weeks),and good selectivity(>80%)for the above ions.In vitro detection and in vivo subcutaneous and brain experiment results showed that the MMEC system exhibits good multi-ion monitoring performance in several complex environments.This work provides a platform for the continuous real-time monitoring of ion fluctuations in different situations and has implications for developing smart sensors to monitor human health.

Rapid Isolation and Multiplexed Detection of Exosome Tumor Markers Via Queued Beads Combined with Quantum Dots in a Microarray

Tumor-derived exosomes are actively involved in cancer progression and metastasis and have emerged as a promising marker for cancer diagnosis in liquid biopsy.Because of their nanoscale size,complex biogenesis,and methodological limitations related to exosome isolation and detection,advancements in their analysis remain slow.Microfluidic technology offers a better analytic approach compared with conventional methods.Here,we developed a bead-based microarray for exosome isolation and multiplexed tumor marker detection.Using this method,exosomes are isolated by binding to antibodies on the bead surface,and tumor markers on the exosomes are detected through quantum dot(QD)probes.The beads are then uniformly trapped and queued among micropillars in the chip.This design benefits fluorescence observation by dispersing the signals into every single bead,thereby avoiding optical interference and enabling more accurate test results.We analyzed exosomes in the cell culture supernatant of lung cancer and endothelial cell lines,and different lung cancer markers labeled with three QD probes were used to conduct multiplexed detection of exosome surface protein markers.Lung cancer-derived samples showed much higher(~sixfold-tenfold)fluorescence intensity than endothelial cell samples,and different types of lung cancer samples showed distinctive marker expression levels.Additionally,using the chip to detect clinical plasma samples from cancer patients showed good diagnostic power and revealed a well consistency with conventional tests for serological markers.These results provide insight into a promising method for exosome tumor marker detection and early-stage cancer diagnosis.

Upconversion Nanoparticles-Encoded Hydrogel Microbeads-Based Multiplexed Protein Detection

Fluorescently encoded microbeads are in demand for multiplexed applications in different fields.Compared to organic dye-based commercially available Luminex's x MAP technology, upconversion nanoparticles(UCNPs) are better alternatives due to their large antiStokes shift, photostability, nil background, and single wavelength excitation. Here, we developed a new multiplexed detection system using UCNPs for encoding poly(ethylene glycol) diacrylate(PEGDA) microbeads as well as for labeling reporter antibody. However, to prepare UCNPs-encoded microbeads, currently used swellingbased encapsulation leads to non-uniformity, which is undesirable for fluorescence-based multiplexing. Hence,we utilized droplet microfluidics to obtain encoded microbeads of uniform size, shape, and UCNPs distribution inside. Additionally, PEGDA microbeads lack functionality for probe antibodies conjugation on their surface.Methods to functionalize the surface of PEGDA microbeads(acrylic acid incorporation, polydopamine coating)reported thus far quench the fluorescence of UCNPs. Here,PEGDA microbeads surface was coated with silica followed by carboxyl modification without compromising the fluorescence intensity of UCNPs. In this study, droplet microfluidics-assisted UCNPs-encoded microbeads of uniform shape, size, and fluorescence were prepared.Multiple color codes were generated by mixing UCNPs emitting red and green colors at different ratios prior to encapsulation. UCNPs emitting blue color were used to label the reporter antibody. Probe antibodies were covalently immobilized on red UCNPs-encoded microbeads for specific capture of human serum albumin(HSA) as a model protein. The system was also demonstrated for multiplexed detection of both human C-reactive protein(hCRP) and HSA protein by immobilizing anti-h CRP antibodies on green UCNPs.

Impact of multiplexed reading scheme on nanocrossbar memristor memory's scalability

Nanocrossbar is a potential memory architecture to integrate memristor to achieve large scale and high density mem- ory. However, based on the currently widely-adopted parallel reading scheme, scalability of the nanocrossbar memory is limited, since the overhead of the reading circuits is in proportion with the size of the nanocrossbar component. In this paper, a multiplexed reading scheme is adopted as the foundation of the discussion. Through HSPICE simulation, we reanalyze scalability of the nanocrossbar memristor memory by investigating the impact of various circuit parameters on the output voltage swing as the memory scales to larger size. We find that multiplexed reading maintains sufficient noise margin in large size nanocrossbar memristor memory. In order to improve the scalability of the memory, memristors with nonlinear I-V characteristics and high LRS （low resistive state） resistance should be adopted.

Remote structural health monitoring with serially multiplexed fiber optic acoustic emission sensors

Development and testing of a serially multiplexed fiber optic sensor system is described.The sensor differs from conventional fiber optic acoustic systems,as it is capable of sensing AE emissions at several points along the length of a single fiber.Multiplexing provides for single channel detection of cracks and their locations in large structural systems. An algorithm was developed for signal recognition and tagging of the AE waveforms for detection of' crack locations,Labora- tory experiments on plain concrete beams and post-tensioned FRP tendons were pcrlormed to evaluate the crack detection capability of the sensor system.The acoustic emission sensor was able to detect initiation,growth and location of the cracks in concrete as well as in the FRP tendons.The AE system is potentially suitable lot applications involving health monitoring of structures following an earthquake.

Dark diffusional enhancement of holographic multiplexed gratings in phenanthrenequinone doped poly(methyl methacrylate) photopolymer

In this paper, we experimentally investigate the dark diffusional enhancement of the optimized multiplexed grating in the phenanthrenequinone doped poly （methyl methacrylate） （PQ-PMMA） photopolymer. The possibility of improving the holographic characteristics of the material through the dark enhancement is demonstrated. The optimal preillumination exposure and the optimal time interval between exposures are extracted to obtain the optimized diffraction efficiency, and their values are 3.4×103 mJ/cm2 and 2 min, respectively. The dark enhancement of the multiplexed grating is presented as an effective method to improve the response region and the dynamic range and to prevent saturation of the material. The dependence of the phenanthrenequinone concentration on the increment of the refractive index modulation is quantitatively studied, which provides a significant basis for improving the homogeneity in the multiplexed gratings using a quantitative strategy. Finally, a simple experimental procedure using the dark enhancement is introduced to improve the homogeneity of the diffraction efficiency and to avoid the complex schedule exposure.

Turbulence mitigation scheme based on multiple-user detection in an orbital-angular-momentum multiplexed system

Atmospheric turbulence（AT） induced crosstalk can significantly impair the performance of a free-space optical（FSO）communication link using orbital angular momentum（OAM） multiplexing.In this paper,we propose a multiple-user detection（MUD） turbulence mitigation scheme in an OAM-multiplexed FSO communication link.First,we present a MUD equivalent communication model for an OAM-multiplexed FSO communication link under AT.In the equivalent model,each input bit stream represents one user＇s information.The deformed OAM spatial modes caused by AT,instead of the pure OAM spatial modes,are used as information carriers,and the overlapping between the deformed OAM spatial modes are computed as the correlation coefficients between the users.Then,we present a turbulence mitigation scheme based on MUD idea to enhance AT tolerance of the OAM-multiplexed FSO communication link.In the proposed scheme,the crosstalk caused by AT is used as a useful component to deduce users＇ information.The numerical results show that the performance of the OAM-multiplexed communication link has greatly improved by the proposed scheme.When the turbulence strength C_n^2 is 1 × 10^（-15） m^（-2/3）,the transmission distance is 1000 m and the channel signal-to-noise ratio（SNR）is 26 dB,the bit-error-rate（BER） performance of four spatial multiplexed OAM modes l_m = ＋ 1,＋2,＋3,＋4 are all close to 10-5,and there is a 2-3 fold increase in the BER performance in comparison with those results without the proposed scheme.In addition,the proposed scheme is more effective for an OAM-multiplexed FSO communication link with a larger OAM mode topological charge interval.The proposed scheme is a promising direction for compensating the interference caused by AT in the OAM-multiplexed FSO communication link.

Multiplexed Profiling of Extracellular Vesicles for Biomarker Development

Extracellular vesicles(EVs)are cell-derived membranous particles that play a crucial role in molecular trafficking,intercellular transport and the egress of unwanted proteins.They have been implicated in many diseases including cancer and neurodegeneration.EVs are detected in all bodily fluids,and their protein and nucleic acid content offers a means of assessing the status of the cells from which they originated.As such,they provide opportunities in biomarker discovery for diagnosis,prognosis or the stratification of diseases as well as an objective monitoring of therapies.The simultaneous assaying of multiple EV-derived markers will be required for an impactful practical application,and multiplexing platforms have evolved with the potential to achieve this.Herein,we provide a comprehensive overview of the currently available multiplexing platforms for EV analysis,with a primary focus on miniaturized and integrated devices that offer potential step changes in analytical power,throughput and consistency.

Deep‐learning powered whispering gallery mode sensor based on multiplexed imaging at fixed frequency

During the last decades the whispering gallery mode based sensors have become a prominent solution for label-free sensing of various physical and chemical parameters.At the same time,the widespread utilization of the approach is hindered by the restricted applicability of the known configurations for ambient variations quantification outside the laboratory conditions and their low affordability,where necessity on the spectrally-resolved data collection is among the main limiting factors.In this paper we demonstrate the first realization of an affordable whispering gallery mode sensor powered by deep learning and multi-resonator imaging at a fixed frequency.It has been shown that the approach enables refractive index unit(RIU)prediction with an absolute error at 3×10^(-6) level for dynamic range of the RIU variations from 0 to 2×10^(-3) with temporal resolution of several milliseconds and instrument-driven detection limit of 3×10−5.High sensing accuracy together with instrumental affordability and production simplicity places the reported detector among the most cost-effective realizations of the whispering gallery mode approach.The proposed solution is expected to have a great impact on the shift of the whole sensing paradigm away from the model-based and to the flexible self-learning solutions.

STUDY ON STATISTICALLY-FAIR SERVICE IN STATISTICALLY-MULTIPLEXED NETWORKS

A new type of fair service, referred to as Statistically-Fair Service (SFS), is proposed in this paper. The SFS discipline is given based on the SFS criterion. Compared to "strict" fair service available, SFS is mainly characterized by its flexible suitability for the nature of statistically-multiplexed networks. By its statistically-fair service to users, therefore, SFS can ensure well end-to-end QoS requirements on a statistical basis with a benefit of enhancement in network utilization. Two useful properties of SFS is presented. One of them, the property of retaining Exponentially Bounded Burstiness(EBB), can facilitate end-to-end delay estimation of EBB-type traffic. Finally, some numerical results obtained from a simulation study on SFS shows that an SFS-equipped node in steady states will in deed retain the EBB attribute of any input flow.

Multi-dimensional multiplexing optical secret sharing framework with cascaded liquid crystal holograms

Secret sharing is a promising technology for information encryption by splitting the secret information into different shares.However,the traditional scheme suffers from information leakage in decryption process since the amount of available information channels is limited.Herein,we propose and demonstrate an optical secret sharing framework based on the multi-dimensional multiplexing liquid crystal(LC)holograms.The LC holograms are used as spatially separated shares to carry secret images.The polarization of the incident light and the distance between different shares are served as secret keys,which can significantly improve the information security and capacity.Besides,the decryption condition is also restricted by the applied external voltage due to the variant diffraction efficiency,which further increases the information security.In implementation,an artificial neural network(ANN)model is developed to carefully design the phase distribution of each LC hologram.With the advantage of high security,high capacity and simple configuration,our optical secret sharing framework has great potentials in optical encryption and dynamic holographic display.

Implementation of Phase Generated Carrier Technique for FBG Laser Sensor Multiplexed System Based on Compact RIO

This paper presents the fundamental technique of phase generated carrier （PGC） and its realization on compact reconfigurable input and output （RIO） which adopts real-time and field programmable grate array （FPGA） techniques. Improvement of the PGC technique is also introduced by using peak-to-peak value detection method to reduce the influence of variation of the light intensity. A four-element fibre Bragg gratings （FBG） laser sensor system is conducted in the experiment and the demodulated results demonstrate correlation coefficient as high as 0.995 with the reference signal and the dynamic range to be 120dB@63Hz.

Study on Distribution of Four Pseudomonas Species in Living Environment Using Multiplex PCR

Purpose: The genus Pseudomonas is a ubiquitous microorganism frequently detected from immunocompromised patients. The inherent resistance to numerous antimicrobial agents contributes to the opportunistic character of this pathogen exhaustive monitoring of this pathogen is considered of critical importance to public health organizations. The reliable identification method able to distinguish genetic close Pseudomonas species is needed, because these organisms are difficult to differentiate by phenotypic or biochemical methods. The purpose of the present study was to design species-specific primers in order to identify and detect four Pseudomonas species which are frequently detected from the human oral cavities, and to investigate the distribution of these organisms in the living environment using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the rpoD gene of four Pseudomonas species. Swab samples were collected from fifty washstands, and the distribution of Pseudomonas species was investigated using a conventional PCR at genus level and a multiplex PCR at species level. Results: Multiplex PCR method developed in this study was able to distinguish four Pseudomonas species clearly. The genus Pseudomonas was detected from all samples (100%), whereas P. putida, P, aeruginosa, P. stutzeri and P. fluorescens were detected at 44%, 8%, 4% and 2% in fifty swab samples, respectively. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction. It was indicated that washstands were the uninhabitable environment for P. putida, P, aeruginosa, P. stutzeri and P. fluorescens.

Real-Time 4-Mode MDM Transmission Using Commercial 400G OTN Transceivers and All-Fiber Mode Multiplexers

Weakly-coupled mode division multiplexing(MDM)technique is considered a promising candidate to enhance the capacity of an optical transmission system,in which mode multiplexers/demultiplexers(MMUX/MDEMUX)with low insertion loss and modal crosstalk are the key components.In this paper,a low-modal-crosstalk 4-mode MMUX/MDEMUX for the weakly-coupled triple-ring-core few-mode fiber(TRC-FMF)is designed and fabricated with side-polishing processing.The measurement results show that a pair of MMUX/MDEMUX and 25 km weakly-coupled TRC-FMF MDM link achieve low modal crosstalk of lower than−17.5 dB and insertion loss of lower than 11.56 dB for all the four modes.Based on the TRC-FMF and all-fiber MMUX/MDEMUX,an experiment for 25 km real-time 4-mode 3-λwavelength division multiplexing(WDM)-MDM transmission is conducted using commercial 400G optical transport network(OTN)transceivers.The experimental results prove weakly-coupled MDM techniques facilitate a smooth upgrade of the optical transmission system.

A High Spectral Efficient Frequency-Domain Channel-Estimation Method for the Polarization-Division-Multiplexed CO-OFDM-OQAM System

Contrary to the other multi-carrier modulation systems, the coherent optical orthogonal frequency division multiplexing communication system with an offset quadrature amplitude modulation (CO-OFDM-OQAM) possesses inherent imaginary interference (IMI). This has an important impact on the channel estimation process. Currently, a variety of frequency-domain channel estimation methods have been proposed. However, there are various problems that still exist. For instance, in order to reduce the influence of IMI, it is necessary to insert more guard intervals between the training sequence and the payload, leading to the occupation of excessive spectrum resources. In order to address this problem, this work designs a high spectral efficient frequency-domain channel estimation method for the polarization-division-multiplexing CO-OFDM-OQAM systems. First, the working principle of the proposed method is described in detail. Then, its spectral efficiency, power peak-to-average ratio, and channel estimation performance are studied based on simulations. The simulation results show that the proposed method improves the spectral efficiency without worsening the power peak-to-average ratio. The channel estimation capability of this method is verified in three scenarios of long-distance transmissions, including back-to-back, 100 km, and 200 km transmissions. .

Fiber Laser Based Multiplexed Sensing System

A laser sensing system based on beat frequency demodulation is proposed. The sensor uses a single-longitudinal-mode distributed Bragg reflector （DBR） fiber laser as a sensing element. This laser sensor has great multiplexing capability due to its wide free spectral range. Wavelength-division-multiplex （WDM） and frequency-division-multiplex （FDM） techniques are studied. The sensing system has high sensitivity and multiplexing channels.

Research on Raman Crosstalk in Broadband Wavelength Division Multiplexed Systems

After a theoretical model is put forward on the base of accurate description of the Raman gain profile and the physical quantity, maximum Raman crosstalk(MRC), which quantificationally depicts the intensity of Raman crosstalk is defined. The influences of launch power, fiber effective core area, fiber nonlinear index, fiber length, channel number and channel interval on MRC are deduced. The result indicates that compared with low speed and narrowband optical fiber communication system, serious Raman crosstalk lies in high speed and broadband system, which impacts the performance of the system badly. The result is useful for forecasting Raman crosstalk in broadband and high speed optical fiber communication system.

A Study of Radiation-Induced Telomere Instability Using Multiplex Ligation-Dependent Probe Amplification (MLPA)

The integrity of the chromosomes for two WIL2-derived lymphoblastoid cell lines (TK6 and WTK1) in the presence and absence of ionizing radiation was analyzed by Multiplex Ligation-Dependent Probe Amplification (MLPA). The TK6 cell line has the native p53 tumor-suppressor gene, whereas WTK1 cells contain a p53 mutation. Each cell line was isolated pre- and post-irradiation (2 and 3 Gy) and analyzed by MLPA. The impact of irradiation on these two cell lines was investigated using probes that target specific regions on chromosomes associated with subtelomeric regions. Results indicate that WTK1 and TK6 are impacted differently after irradiation, and that each cell line presents its own unique MLPA profile. The most notable differences are the appearance of a number of probes in the post-irradiated MLPA profile that are not present in the controls, and two unique probe signals only seen in WTK1 cells. These results build on our previous studies that indicate how different human cell lines can be affected by radiation in significantly different ways depending on the presence or absence of wild type p53.

Combining Polarization-Division Multiplexing and Ferromagnetic Nonreciprocity to Achieve In-Band Ultra-High Isolation for Full-Duplex Wireless Systems

The in-band full-duplex(IBFD)wireless system is a promising candidate for 6G and beyond,as it can double data throughput and enormously lower transmission latency by supporting simultaneous in-band transmission and reception of signals.Enabling IBFD systems requires a substantial mitigation of a transmitter(Tx)’s strong self-interference(SI)signal into the receiver(Rx)channel.However,current state-ofthe-art approaches to tackle this challenge are inefficient in terms of performance,cost,and complexity,hindering the commercialization of IBFD techniques.In this work,we devise and demonstrate an innovative approach to realize IBFD systems that exhibit superior performance with a low-cost and lesscomplex architecture in an all-passive module.Our scheme is based on meticulously combining polarization-division multiplexing(PDM)with ferromagnetic nonreciprocity to achieve ultra-high isolation between Tx and Rx channels.Such an unprecedented conception has become feasible thanks to a concurrent dual-mode circulator—a new component introduced for the first time—as a key feature of our module,and a dual-mode waveguide that transforms two orthogonally polarized waves into two orthogonal waveguide modes.In addition,we propose a unique passive tunable secondary SI cancellation(SIC)mechanism,which is embedded within the proposed module and boosts the isolation over a relatively broad bandwidth.We report,solely in the analog domain,experimental isolation levels of 50,70,and 80 dB over 340,101,and 33 MHz bandwidth at the center frequency of interest,respectively,with excellent tuning capability.Furthermore,the module is tested in two real IBFD scenarios to assess its performance in connection with Tx-to-Rx leakage and modulation error in the presence of a Tx’s strong interference signal.