The aims of this work were: To achieve a simple and low cost propagation of potential probiotic agents using plain whey as a culture medium, study the diversity of the members of the bacterial community (MC) in plain ...The aims of this work were: To achieve a simple and low cost propagation of potential probiotic agents using plain whey as a culture medium, study the diversity of the members of the bacterial community (MC) in plain whey and to evaluate the probiotic capacity of this MC. After a systematic selection of agents according to their growing capacity in whey, the constituted MC was considered as a unit. Biochemical characterization of the lactic acid bacteria were performed using the API system. Molecular characterization of the lactic acid bacteria was realized using AFLPTM DNA-fingerprinting, partial 16S rDNA sequence analysis and PCR-denaturing gradient gel electrophoresis (PCR-DGGE). The physiological characterization of yeast was determined with the automated microplate method Allev/Biolomics and using yeast characterization system based on standard taxonomic criteria. The identification molecular was realized by PCR-fingerprinting. The resistance of MC to pH and bile salts were evaluated. The MC was composed of agents from different separated Dominium like Bacteria (Lactobacillum) and Eukaria (yeast). They are multispecies and also multistrain assuring high biodiversity. The MC grew at low pH and different concentrations bile salts.展开更多
In this research,we introduce a comprehensive epidemiological model that accounts for multiple strains of an infectious disease and two distinct vaccination options.Vaccination stands out as the most effective means t...In this research,we introduce a comprehensive epidemiological model that accounts for multiple strains of an infectious disease and two distinct vaccination options.Vaccination stands out as the most effective means to prevent and manage infectious diseases.However,when there are various vaccines available,each with its costs and effectiveness,the decision-making process for individuals becomes paramount.Furthermore,the factor of waning immunity following vaccination also plays a significant role in influencing these choices.To understand how individuals make decisions in the context of multiple strains and waning immunity,we employ a behavioral model,allowing an epidemiological model to be coupled with the dynamics of a decision-making process.Individuals base their choice of vaccination on factors such as the total number of infected individuals and the cost-effectiveness of the vaccine.Our findings indicate that as waning immunity increases,people tend to prioritize vaccines with higher costs and greater efficacy.Moreover,when more contagious strains are present,the equilibrium in vaccine adoption is reached more rapidly.Finally,we delve into the social dilemma inherent in our model by quantifying the social efficiency deficit(SED)under various parameter combinations.展开更多
文摘The aims of this work were: To achieve a simple and low cost propagation of potential probiotic agents using plain whey as a culture medium, study the diversity of the members of the bacterial community (MC) in plain whey and to evaluate the probiotic capacity of this MC. After a systematic selection of agents according to their growing capacity in whey, the constituted MC was considered as a unit. Biochemical characterization of the lactic acid bacteria were performed using the API system. Molecular characterization of the lactic acid bacteria was realized using AFLPTM DNA-fingerprinting, partial 16S rDNA sequence analysis and PCR-denaturing gradient gel electrophoresis (PCR-DGGE). The physiological characterization of yeast was determined with the automated microplate method Allev/Biolomics and using yeast characterization system based on standard taxonomic criteria. The identification molecular was realized by PCR-fingerprinting. The resistance of MC to pH and bile salts were evaluated. The MC was composed of agents from different separated Dominium like Bacteria (Lactobacillum) and Eukaria (yeast). They are multispecies and also multistrain assuring high biodiversity. The MC grew at low pH and different concentrations bile salts.
基金This study received financial support in the form of a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science(JSPS),specifically through KAKENHI(Grant No.JP 23H03499).
文摘In this research,we introduce a comprehensive epidemiological model that accounts for multiple strains of an infectious disease and two distinct vaccination options.Vaccination stands out as the most effective means to prevent and manage infectious diseases.However,when there are various vaccines available,each with its costs and effectiveness,the decision-making process for individuals becomes paramount.Furthermore,the factor of waning immunity following vaccination also plays a significant role in influencing these choices.To understand how individuals make decisions in the context of multiple strains and waning immunity,we employ a behavioral model,allowing an epidemiological model to be coupled with the dynamics of a decision-making process.Individuals base their choice of vaccination on factors such as the total number of infected individuals and the cost-effectiveness of the vaccine.Our findings indicate that as waning immunity increases,people tend to prioritize vaccines with higher costs and greater efficacy.Moreover,when more contagious strains are present,the equilibrium in vaccine adoption is reached more rapidly.Finally,we delve into the social dilemma inherent in our model by quantifying the social efficiency deficit(SED)under various parameter combinations.
