Plant prevacuolar compartments （PVCs）, or multivesicular bodies （MVBs）, are single membrane-bound organelles that play important roles in mediating protein trafficking to vacuoles in the secretory pathway. PVC/MVB...Plant prevacuolar compartments （PVCs）, or multivesicular bodies （MVBs）, are single membrane-bound organelles that play important roles in mediating protein trafficking to vacuoles in the secretory pathway. PVC/MVB also serves as a late endosome in the endocytic pathway in plants. Since the plant PVC was iden- tified as an MVB more than 10 years ago,-great progress has been made toward the understanding of PVC/ MVB function and biogenesis in plants. In this review, we first summarize previous research into the iden- tification and characterization of plant PVCs/MVBs, and then highlight recent advances on the mechanisms underlying intraluminal vesicle formation and maturation of plant PVCs/MVBs. In addition, we discuss the possible crosstalk that appears to occur between PVCs/MVBs and autophagosomes during autophagy in plants. Finally, we list some open questions and present future perspectives in this field.展开更多
A combinational therapeutic system that simultaneously administrates various pathways is preferred for anti-cancer treatment.In the present study,we successfully constructed a co-delivery system,multivesicular liposom...A combinational therapeutic system that simultaneously administrates various pathways is preferred for anti-cancer treatment.In the present study,we successfully constructed a co-delivery system,multivesicular liposomes(MVLs)co-encapsulating doxorubicin(DOX)and luminespib(AUY922).A simple and accurate dual-wavelength spectrophotometric method was established for the determination of DOX and AUY922 in liposomal formulation.MVL-loading drugs were prepared by a multi-emulsion solvent evaporation method,which exhibited excellent physicochemical properties,such as particle size of 3–8μm and high entrapment efficiency above 95%for DOX and 73%for AUY922.The synergetic cytotoxic effect for these two drugs was evaluated in MDA-MB-231 cells.The in vitro antitumor studies demonstrated the superior anti-proliferation activity of DOX and AUY922 with a combination index of 0.43,indicating a great synergistic effect.The experimental data suggested that combinational use of DOX and AUY922 within liposomes could be an effective way to develop efficient treatments of cancers.展开更多
文摘Plant prevacuolar compartments （PVCs）, or multivesicular bodies （MVBs）, are single membrane-bound organelles that play important roles in mediating protein trafficking to vacuoles in the secretory pathway. PVC/MVB also serves as a late endosome in the endocytic pathway in plants. Since the plant PVC was iden- tified as an MVB more than 10 years ago,-great progress has been made toward the understanding of PVC/ MVB function and biogenesis in plants. In this review, we first summarize previous research into the iden- tification and characterization of plant PVCs/MVBs, and then highlight recent advances on the mechanisms underlying intraluminal vesicle formation and maturation of plant PVCs/MVBs. In addition, we discuss the possible crosstalk that appears to occur between PVCs/MVBs and autophagosomes during autophagy in plants. Finally, we list some open questions and present future perspectives in this field.
基金School of Pharmaceutical Sciences,Peking University Health Science Center,Beijing 100191,China。
文摘A combinational therapeutic system that simultaneously administrates various pathways is preferred for anti-cancer treatment.In the present study,we successfully constructed a co-delivery system,multivesicular liposomes(MVLs)co-encapsulating doxorubicin(DOX)and luminespib(AUY922).A simple and accurate dual-wavelength spectrophotometric method was established for the determination of DOX and AUY922 in liposomal formulation.MVL-loading drugs were prepared by a multi-emulsion solvent evaporation method,which exhibited excellent physicochemical properties,such as particle size of 3–8μm and high entrapment efficiency above 95%for DOX and 73%for AUY922.The synergetic cytotoxic effect for these two drugs was evaluated in MDA-MB-231 cells.The in vitro antitumor studies demonstrated the superior anti-proliferation activity of DOX and AUY922 with a combination index of 0.43,indicating a great synergistic effect.The experimental data suggested that combinational use of DOX and AUY922 within liposomes could be an effective way to develop efficient treatments of cancers.
