As a non-specific modulator of macrophage, multiplied muramyl dipeptide (MMD) is solid-phase synthesized by application of standard Fmoc chemistry strategy. Tarn's multiple antigen system (MAS) is used as our four...As a non-specific modulator of macrophage, multiplied muramyl dipeptide (MMD) is solid-phase synthesized by application of standard Fmoc chemistry strategy. Tarn's multiple antigen system (MAS) is used as our four branched-linker on Lysine.展开更多
The solution-phase synthesis of a muramyl dipeptide（MDP） analogue of N~α-[4-chlorocinnamoyl-L-alanyl-D-isoglutaminyl]-L-lysine （MDA,2） is reported that possesses the features of easy feasibility,safety and low co...The solution-phase synthesis of a muramyl dipeptide（MDP） analogue of N~α-[4-chlorocinnamoyl-L-alanyl-D-isoglutaminyl]-L-lysine （MDA,2） is reported that possesses the features of easy feasibility,safety and low cost in large scale of synthesis.展开更多
Commensal bacteria boost serum IgG production in response to oral immunization with antigen and cholera toxin(CT)in a manner that depends on Nod2(nucleotide-binding oligomerization domain-containing protein 2).In this...Commensal bacteria boost serum IgG production in response to oral immunization with antigen and cholera toxin(CT)in a manner that depends on Nod2(nucleotide-binding oligomerization domain-containing protein 2).In this study,we examined the role of intestinal lysozyme(Lyz1)in adjuvant activity of CT.We found that Lyz1 released Nod2 ligand(s)from bacteria.Lyz1 deficiency reduced the level of circulating Nod2 ligand in mice.Lyz1 deficiency also reduced the production of IgG and T-cellspecific cytokines after oral immunization in mice.Supplementing Lyz1-deficient mice with MDP restored IgG production.Furthermore,overexpression of Lyz1 in intestinal epithelium boosted the antigen-specific IgG response induced by CT.Collectively,our results indicate that Lyz1 plays an important role in mediating the immune regulatory effect of commensal bacteria through the release of Nod2 ligand(s).展开更多
基金These studies were granted by the National Natural Science Foundation of China (39600183).
文摘As a non-specific modulator of macrophage, multiplied muramyl dipeptide (MMD) is solid-phase synthesized by application of standard Fmoc chemistry strategy. Tarn's multiple antigen system (MAS) is used as our four branched-linker on Lysine.
基金supported financially by the National Natural Science Foundation of China(No.90713045)
文摘The solution-phase synthesis of a muramyl dipeptide（MDP） analogue of N~α-[4-chlorocinnamoyl-L-alanyl-D-isoglutaminyl]-L-lysine （MDA,2） is reported that possesses the features of easy feasibility,safety and low cost in large scale of synthesis.
基金supported by the National Key Research and Development Program of China(2017YFA0503403)the National Natural Science Foundation of China(31730028 and 81670482)the Key Program for Frontier Science of the Chinese Academy of Sciences(QYZDBSSW-SMC025).
文摘Commensal bacteria boost serum IgG production in response to oral immunization with antigen and cholera toxin(CT)in a manner that depends on Nod2(nucleotide-binding oligomerization domain-containing protein 2).In this study,we examined the role of intestinal lysozyme(Lyz1)in adjuvant activity of CT.We found that Lyz1 released Nod2 ligand(s)from bacteria.Lyz1 deficiency reduced the level of circulating Nod2 ligand in mice.Lyz1 deficiency also reduced the production of IgG and T-cellspecific cytokines after oral immunization in mice.Supplementing Lyz1-deficient mice with MDP restored IgG production.Furthermore,overexpression of Lyz1 in intestinal epithelium boosted the antigen-specific IgG response induced by CT.Collectively,our results indicate that Lyz1 plays an important role in mediating the immune regulatory effect of commensal bacteria through the release of Nod2 ligand(s).
