Murraya exotica L. is one of the two official source species of traditional Chinese medicine Murrayae Folium et Cacumen. At present, a rapid HPLC analysis method to simultaneously determine two major coumarins, hainan...Murraya exotica L. is one of the two official source species of traditional Chinese medicine Murrayae Folium et Cacumen. At present, a rapid HPLC analysis method to simultaneously determine two major coumarins, hainanmurpanin （1） and meranzin （2）, and one main flavonoid, 3,5,6,7,3＇,4＇,5＇-heptamethoxyflavone （3）, from the leaves and twigs of M. exotica was established. The analysis was performed on a DIKMA Spursil C18 column （4.6 minx250 mm, 5 ~m） at a flow rate of 1 mL/min, using acetonitrile-H20 （5：5, v/v） as mobile phase. The column temperature was 25 ^（2, and the detected wavelength was at 320 nm. The three analytes （1-3） were separated well with good linearity, precision, stability and repeatability. The average recoveries were in the range of 100.52%-101.97%, with RSD less than 1.73%. Twenty batches of M. exotica from different habitats were detected, and the sum of the contents of 1-3 was in the range of 1.55-7.45 mg/g, respectively. Besides, the contents of these three analytes were also determined for the samples from different medicinal parts （stems, lateral branches, mixture of twigs and leaves） and different harvest times. The results showed that the contents of these three analytes in leaves and twigs are much higher than those in the stems or lateral branches; the plants harvested from June or October contain more active compounds than those from the other months. The above results proved that this high performance and simple HPLC assay can be readily utilized as a practical method for the quality control of M. exotica.展开更多
A comparison of the pharmacodynamic effects of two source plants of Murrayae Folium et Cacumen(MFC),Murraya exotica L.and Murraya paniculata(L.)Jack,was performed in order to supply reference for its multi-source rati...A comparison of the pharmacodynamic effects of two source plants of Murrayae Folium et Cacumen(MFC),Murraya exotica L.and Murraya paniculata(L.)Jack,was performed in order to supply reference for its multi-source rationality and interchangeability in clinical practice.According to the traditional efficacy of MFC,the effects of promoting Qi,relieving pain,promoting blood circulation and removing blood stasis were systematically evaluated by the models of writhing response in mice,foot swelling in rats,gastric emptying and small intestine propulsion in mice,and acute blood stasis in rats,respectively.The results showed that both M.exotica and M.paniculata could significantly inhibit the writhing reaction induced by acetic acid in mice and the paw swelling induced by carrageenan in rats,reduce IL-6,TNF-αand PGE2 levels in plasma of paw-swelling rats and increase gastric empty rate and intestinal propulsive rate.The above-mentioned effects were dose-dependent,and there was no significant difference between M.exotica and M.paniculata at the same doses.Therefore,M.exotica and M.paniculata had the similar anti-inflammatory,analgesic and gastrointestinal motility promotion effects,which provided a support for the pharmacodynamic equivalence of the multi-source plants of MFC.展开更多
基金National Natural Science Foundation of China(Grant No.81222051 and 81473106)National Key Technology R&D Program"New Drug Innovation"of China(Grant No.2012ZX09301002-002-002 and 2012ZX09304-005)
文摘Murraya exotica L. is one of the two official source species of traditional Chinese medicine Murrayae Folium et Cacumen. At present, a rapid HPLC analysis method to simultaneously determine two major coumarins, hainanmurpanin （1） and meranzin （2）, and one main flavonoid, 3,5,6,7,3＇,4＇,5＇-heptamethoxyflavone （3）, from the leaves and twigs of M. exotica was established. The analysis was performed on a DIKMA Spursil C18 column （4.6 minx250 mm, 5 ~m） at a flow rate of 1 mL/min, using acetonitrile-H20 （5：5, v/v） as mobile phase. The column temperature was 25 ^（2, and the detected wavelength was at 320 nm. The three analytes （1-3） were separated well with good linearity, precision, stability and repeatability. The average recoveries were in the range of 100.52%-101.97%, with RSD less than 1.73%. Twenty batches of M. exotica from different habitats were detected, and the sum of the contents of 1-3 was in the range of 1.55-7.45 mg/g, respectively. Besides, the contents of these three analytes were also determined for the samples from different medicinal parts （stems, lateral branches, mixture of twigs and leaves） and different harvest times. The results showed that the contents of these three analytes in leaves and twigs are much higher than those in the stems or lateral branches; the plants harvested from June or October contain more active compounds than those from the other months. The above results proved that this high performance and simple HPLC assay can be readily utilized as a practical method for the quality control of M. exotica.
基金National Natural Science Foundation of China(Grant Nos.81773864,81473106)National Key R&D Program of China(Grant No.2019YFC1711000)。
文摘A comparison of the pharmacodynamic effects of two source plants of Murrayae Folium et Cacumen(MFC),Murraya exotica L.and Murraya paniculata(L.)Jack,was performed in order to supply reference for its multi-source rationality and interchangeability in clinical practice.According to the traditional efficacy of MFC,the effects of promoting Qi,relieving pain,promoting blood circulation and removing blood stasis were systematically evaluated by the models of writhing response in mice,foot swelling in rats,gastric emptying and small intestine propulsion in mice,and acute blood stasis in rats,respectively.The results showed that both M.exotica and M.paniculata could significantly inhibit the writhing reaction induced by acetic acid in mice and the paw swelling induced by carrageenan in rats,reduce IL-6,TNF-αand PGE2 levels in plasma of paw-swelling rats and increase gastric empty rate and intestinal propulsive rate.The above-mentioned effects were dose-dependent,and there was no significant difference between M.exotica and M.paniculata at the same doses.Therefore,M.exotica and M.paniculata had the similar anti-inflammatory,analgesic and gastrointestinal motility promotion effects,which provided a support for the pharmacodynamic equivalence of the multi-source plants of MFC.
