Cold stress is an environmental factor affecting plant development and production. Recently,micro RNAs(mi RNAs) have been found to be involved in several plant processes such as growth regulation and stress responses....Cold stress is an environmental factor affecting plant development and production. Recently,micro RNAs(mi RNAs) have been found to be involved in several plant processes such as growth regulation and stress responses. Although mi RNAs and their targets have been identified in several banana species, their participation during cold accumulation in banana remains unknown. In this study, two small RNA libraries were generated from micropropagated plantlets of Musa balbisiana grown at normal and low temperature(5°C).A total of 69 known mi RNAs and 32 putative novel mi RNAs were detected in the libraries by Solexa sequencing. Sixty-four cold-inducible mi RNAs were identified through differentially expressed mi RNAs analysis. Among 43 mi RNAs belonging to 26 conserved mi RNA families with altered expression, 18 were upregulated and 25 downregulated under cold stress. Of21 putative novel mi RNAs with altered expression, four were downregulated and 17 upregulated. Furthermore,eight mi RNAs were validated by stem-loop q RT-PCR and their dynamic differential expression was analyzed. In addition, 393 target genes of 58 identified cold-responsive mi RNAs were predicted and categorized by function.These results provide important information for further characterization and functional analysis of cold-responsive mi RNAs in banana.展开更多
新菠萝灰粉蚧是一种多食性昆虫。在恒温(25℃)条件下,研究并组建了新菠萝灰粉蚧在剑麻(Agavesisalana)、金边龙舌兰(Agave americana var.marginata)、仙人掌(Opuntia dillenii)和香蕉(Musa balbisiana)4种寄主植物上的实验种群生命表...新菠萝灰粉蚧是一种多食性昆虫。在恒温(25℃)条件下,研究并组建了新菠萝灰粉蚧在剑麻(Agavesisalana)、金边龙舌兰(Agave americana var.marginata)、仙人掌(Opuntia dillenii)和香蕉(Musa balbisiana)4种寄主植物上的实验种群生命表。结果表明,在上述4种寄主上新菠萝灰粉蚧发育历期及产仔量均有差异。种群趋势指数(I)分别为73.313、65.378、41.689、65.262。综合比较4种寄主植物,剑麻、金边龙舌兰和香蕉3种为新菠萝灰粉蚧的适宜寄主。展开更多
基金supported by the grants from the National Natural Science Foundation of China (31501043)the Ministry of Science and Technology of the People’s Republic of China (2011AA10020605)the Earmarked Fund for Modern Agro-industry Technology Research System (CARS-32)
文摘Cold stress is an environmental factor affecting plant development and production. Recently,micro RNAs(mi RNAs) have been found to be involved in several plant processes such as growth regulation and stress responses. Although mi RNAs and their targets have been identified in several banana species, their participation during cold accumulation in banana remains unknown. In this study, two small RNA libraries were generated from micropropagated plantlets of Musa balbisiana grown at normal and low temperature(5°C).A total of 69 known mi RNAs and 32 putative novel mi RNAs were detected in the libraries by Solexa sequencing. Sixty-four cold-inducible mi RNAs were identified through differentially expressed mi RNAs analysis. Among 43 mi RNAs belonging to 26 conserved mi RNA families with altered expression, 18 were upregulated and 25 downregulated under cold stress. Of21 putative novel mi RNAs with altered expression, four were downregulated and 17 upregulated. Furthermore,eight mi RNAs were validated by stem-loop q RT-PCR and their dynamic differential expression was analyzed. In addition, 393 target genes of 58 identified cold-responsive mi RNAs were predicted and categorized by function.These results provide important information for further characterization and functional analysis of cold-responsive mi RNAs in banana.
文摘新菠萝灰粉蚧是一种多食性昆虫。在恒温(25℃)条件下,研究并组建了新菠萝灰粉蚧在剑麻(Agavesisalana)、金边龙舌兰(Agave americana var.marginata)、仙人掌(Opuntia dillenii)和香蕉(Musa balbisiana)4种寄主植物上的实验种群生命表。结果表明,在上述4种寄主上新菠萝灰粉蚧发育历期及产仔量均有差异。种群趋势指数(I)分别为73.313、65.378、41.689、65.262。综合比较4种寄主植物,剑麻、金边龙舌兰和香蕉3种为新菠萝灰粉蚧的适宜寄主。
