Mitochondrial organelle transplantation (MOT) is an innovative strategy for the treatment of mitochondrial dysfunction such as cardiac ischemic reperfusion injuries, traumatic brain and spinal cord injuries, cerebral ...Mitochondrial organelle transplantation (MOT) is an innovative strategy for the treatment of mitochondrial dysfunction such as cardiac ischemic reperfusion injuries, traumatic brain and spinal cord injuries, cerebral stroke, and neurodegenerative diseases. The earlier MOT results in better efficacy in animal models of urgent diseases such as ischemic stroke, and traumatic brain and spinal cord injuries. There is no long-term method to preserve mitochondria. Routine MOT procedure from cell growth to mitochondrial injection often takes serval weeks and is not satisfactory for urgent use cases. Hypothesis: Cryopreserved cells might be mitochondrial donors for MOT. Methods: We isolated mitochondria from cryopreserved human fibroblasts and mesenchymal stem cells (MSCs) in cell banks and compared the mitochondrial viability and transplantation with the mitochondria from fresh cells. Key findings: We found that mitochondria from fresh and cryopreserved cells are comparable in mitochondrial viability and transplantation. We also obtained data showing that mitochondria of fibroblasts and MSCs had similar membrane potential and transfer ability, but MSC’s mitochondria had higher ATP content than fibroblast’s mitochondria. In addition, oxygen consumption rates (OCRs) were higher in MSC’s mitochondria compared to fibroblast’s mitochondria and did not change between fresh and frozen cells. Conclusion: Cryopreserved fibroblasts and MSCs are alternative mitochondrial donors for MOT to fresh cells. MSCs could provide higher ATP-produced mitochondria than fibroblasts.展开更多
当前电力营销业务已由单一的供电服务逐渐转变为碳交易、客户侧储能、多能供应、能效服务、分布式电源等综合能源服务,及时响应用户需求变得尤为重要。针对传统建设模式中存在的跨环节协作壁垒、业务需求响应不及时等问题,文章借鉴中台...当前电力营销业务已由单一的供电服务逐渐转变为碳交易、客户侧储能、多能供应、能效服务、分布式电源等综合能源服务,及时响应用户需求变得尤为重要。针对传统建设模式中存在的跨环节协作壁垒、业务需求响应不及时等问题,文章借鉴中台架构设计理念,首先提出一种公共共享服务研运一体化支撑平台(middle-platform service components,MSC),利用模型编排、服务编排、流程编排、界面编排进行微服务灵活实现及扩展;然后,结合架构资产统一管理以及MSC四编排引擎,基于多租户技术进行电力营销业务系统研运一体化建设;最后,通过在客户侧工单中心建设中的应用,证明该模式可为电力营销业务系统建设提供有效支撑。展开更多
The Centre for China and Globalisation(CCG)hosted a side event on February 17 titled"China,Europe,and the United States:Climate Cooperation in an Era of Great Power Politics"at the Munich Security Conference...The Centre for China and Globalisation(CCG)hosted a side event on February 17 titled"China,Europe,and the United States:Climate Cooperation in an Era of Great Power Politics"at the Munich Security Conference(MSC)2024,which was convened from February 16 to 18,2024 in Munich,Germany.展开更多
目的观察缺氧预处理骨髓间充质干细胞(MSCs)移植对脑缺血再灌注损伤大鼠SDF-1/CXCR4 m RNA和蛋白表达的影响及清热化瘀方的干预作用。方法采用线栓法制备局灶性脑缺血大鼠(MCAO)模型,将216只SD大鼠随机分为6组:假手术组(SO)、模型组(MC...目的观察缺氧预处理骨髓间充质干细胞(MSCs)移植对脑缺血再灌注损伤大鼠SDF-1/CXCR4 m RNA和蛋白表达的影响及清热化瘀方的干预作用。方法采用线栓法制备局灶性脑缺血大鼠(MCAO)模型,将216只SD大鼠随机分为6组:假手术组(SO)、模型组(MCAO)、MSCs移植对照组(N-MSCs)、经HP处理后的MSCs移植组(HP-MSCs)、MSCs移植联合清热化瘀方组(MSCs+QRHY)、HP-MSCs移植联合清热化瘀方组(HP+QRHY)。每组大鼠36只,每组根据取材时间点7,14,28 d又可分为每组3个亚组,每个亚组12只大鼠。采用q RT-PCR和Western blot观察3个时间点SDF-1/CXCR4 m RNA及其蛋白的表达变化,并以TUNEL法检测神经细胞凋亡。结果各组缺血半暗带SDF-1/CXCR4 m RNA及其蛋白的表达均于7d达到高峰,14,28 d表达逐渐下降。其中7,14 d同一时间点组间比较,HP-MSCs组、MSCs+QRHY组及HP+QRHY组二者的表达均明显优于N-MSCs组(P<0.01,P<0.05),而以HP+QRHY组增高最为明显(P<0.05,P<0.01),28 d后,各移植组的表达趋势趋同,但仍高于模型组(P<0.05)。结论缺氧预处理MSCs移植能够显著提高脑缺血再灌注损伤大鼠SDF-1/CXCR4的表达,清热化瘀方能够进一步上调SDF/1CXCR4的表达,减少细胞凋亡。展开更多
文摘Mitochondrial organelle transplantation (MOT) is an innovative strategy for the treatment of mitochondrial dysfunction such as cardiac ischemic reperfusion injuries, traumatic brain and spinal cord injuries, cerebral stroke, and neurodegenerative diseases. The earlier MOT results in better efficacy in animal models of urgent diseases such as ischemic stroke, and traumatic brain and spinal cord injuries. There is no long-term method to preserve mitochondria. Routine MOT procedure from cell growth to mitochondrial injection often takes serval weeks and is not satisfactory for urgent use cases. Hypothesis: Cryopreserved cells might be mitochondrial donors for MOT. Methods: We isolated mitochondria from cryopreserved human fibroblasts and mesenchymal stem cells (MSCs) in cell banks and compared the mitochondrial viability and transplantation with the mitochondria from fresh cells. Key findings: We found that mitochondria from fresh and cryopreserved cells are comparable in mitochondrial viability and transplantation. We also obtained data showing that mitochondria of fibroblasts and MSCs had similar membrane potential and transfer ability, but MSC’s mitochondria had higher ATP content than fibroblast’s mitochondria. In addition, oxygen consumption rates (OCRs) were higher in MSC’s mitochondria compared to fibroblast’s mitochondria and did not change between fresh and frozen cells. Conclusion: Cryopreserved fibroblasts and MSCs are alternative mitochondrial donors for MOT to fresh cells. MSCs could provide higher ATP-produced mitochondria than fibroblasts.
文摘当前电力营销业务已由单一的供电服务逐渐转变为碳交易、客户侧储能、多能供应、能效服务、分布式电源等综合能源服务,及时响应用户需求变得尤为重要。针对传统建设模式中存在的跨环节协作壁垒、业务需求响应不及时等问题,文章借鉴中台架构设计理念,首先提出一种公共共享服务研运一体化支撑平台(middle-platform service components,MSC),利用模型编排、服务编排、流程编排、界面编排进行微服务灵活实现及扩展;然后,结合架构资产统一管理以及MSC四编排引擎,基于多租户技术进行电力营销业务系统研运一体化建设;最后,通过在客户侧工单中心建设中的应用,证明该模式可为电力营销业务系统建设提供有效支撑。
文摘The Centre for China and Globalisation(CCG)hosted a side event on February 17 titled"China,Europe,and the United States:Climate Cooperation in an Era of Great Power Politics"at the Munich Security Conference(MSC)2024,which was convened from February 16 to 18,2024 in Munich,Germany.
文摘目的观察缺氧预处理骨髓间充质干细胞(MSCs)移植对脑缺血再灌注损伤大鼠SDF-1/CXCR4 m RNA和蛋白表达的影响及清热化瘀方的干预作用。方法采用线栓法制备局灶性脑缺血大鼠(MCAO)模型,将216只SD大鼠随机分为6组:假手术组(SO)、模型组(MCAO)、MSCs移植对照组(N-MSCs)、经HP处理后的MSCs移植组(HP-MSCs)、MSCs移植联合清热化瘀方组(MSCs+QRHY)、HP-MSCs移植联合清热化瘀方组(HP+QRHY)。每组大鼠36只,每组根据取材时间点7,14,28 d又可分为每组3个亚组,每个亚组12只大鼠。采用q RT-PCR和Western blot观察3个时间点SDF-1/CXCR4 m RNA及其蛋白的表达变化,并以TUNEL法检测神经细胞凋亡。结果各组缺血半暗带SDF-1/CXCR4 m RNA及其蛋白的表达均于7d达到高峰,14,28 d表达逐渐下降。其中7,14 d同一时间点组间比较,HP-MSCs组、MSCs+QRHY组及HP+QRHY组二者的表达均明显优于N-MSCs组(P<0.01,P<0.05),而以HP+QRHY组增高最为明显(P<0.05,P<0.01),28 d后,各移植组的表达趋势趋同,但仍高于模型组(P<0.05)。结论缺氧预处理MSCs移植能够显著提高脑缺血再灌注损伤大鼠SDF-1/CXCR4的表达,清热化瘀方能够进一步上调SDF/1CXCR4的表达,减少细胞凋亡。
