期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
苹果属小金海棠转录因子MxMYB1基因的克隆及其原核表达 被引量:5
1
作者 曹冬梅 许雪峰 韩振海 《园艺学报》 CAS CSCD 北大核心 2006年第4期833-835,共3页
MxMYB1是苹果属小金海棠MYB类转录因子。MxMYB1含1个重复序列及MYB蛋白特有的氨基酸组成。酶切、PCR扩增及测序分析表明构建的原核表达载体结构正确,未出现碱基突变及移码现象。1mmol/LIPTG诱导2h后,在预期的蛋白分子量38kD处出现1条表... MxMYB1是苹果属小金海棠MYB类转录因子。MxMYB1含1个重复序列及MYB蛋白特有的氨基酸组成。酶切、PCR扩增及测序分析表明构建的原核表达载体结构正确,未出现碱基突变及移码现象。1mmol/LIPTG诱导2h后,在预期的蛋白分子量38kD处出现1条表达加强的蛋白条带,而未经诱导的转化子没有此蛋白条带。为进一步目的蛋白的纯化和鉴定提供试验基础。 展开更多
关键词 转录因子mxmyb1 原核表达 pET30a-mxmyb1
下载PDF
An MYB Transcription Factor from Malus xiaojinensis Has a Potential Role in Iron Nutrition 被引量:7
2
作者 Jie Shen Xuefeng Xu Tianzhong Li Dongmei Cao ZhenhaiHan 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2008年第10期1300-1306,共7页
Regulation of iron uptake and use is critical for plant survival and growth. We isolated an MYB gene from Malus xiaojinensis named MxMYB1, which is induced under Fe-deficient conditions. Expression of MxMYB1 was upreg... Regulation of iron uptake and use is critical for plant survival and growth. We isolated an MYB gene from Malus xiaojinensis named MxMYB1, which is induced under Fe-deficient conditions. Expression of MxMYB1 was upregulated by Fe starvation in the roots but not in leaves, suggesting that MxMYB1 might play a role in iron nutrition in roots. Transgenic Arabidopsis plants expressing MxMYB1 exhibited lower iron content as compared with wild type plants under both Fe-normal (40 μM) and Fe-deficient conditions (Fe omitted and Ferrozine 300 μM). However, the contents of Cu, Zn and Mn were not changed in these transgenic plants. Gene chip and real-time polymerase chain reaction analyses indicated that the expression of two Fe-related genes encoding an iron transporter AtlRT1 and an iron storage protein ferritin AtFER1 might be negatively regulated by MxMYB1 as the expression levels of these genes were lower in MxMYB1 expressing transgenic Arabidopsis plants as compared with wild type plants under both Fe-normal and Fe-deficient conditions. These results suggest that MxMYB1 may function as a negative regulator of iron uptake and storage in plants. 展开更多
关键词 FE Malus xiaojinensis mxmyb transgenic Arabidopsis.
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部