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PCR-RFLP and Sequence Analysis of Mx Gene 3'Sequence in Langya Chicken Breeds 被引量:6
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作者 刘振国 李桢 王宝维 《Agricultural Science & Technology》 CAS 2009年第6期50-52,共3页
Objective The aim was to provide basis for molecular marker assisted selection and resistance breeding of Langya chicken. Method The genetic polymorphism of Hae III site of Mx gene 3' sequence in Langya chicken was ... Objective The aim was to provide basis for molecular marker assisted selection and resistance breeding of Langya chicken. Method The genetic polymorphism of Hae III site of Mx gene 3' sequence in Langya chicken was analyzed by PCR-RFLP. Result The results showed that Hae III site controlled by allele A and B were polymorphic in Langya chicken breeds and the allele frequencies of A and B were 0.562 and 0.438 respectively. The genotype distribution of Hae III site was significantly not in Hardy-Weinberg equilibrium ( P 〈0.01 ). The polymorphic fragments were cloned and sequenced, and the results revealed that the fragment size was 357 bp and a deletion of 31 bp occurred in variation sequences. Conclusion It was found that Hae III-RFLP exists in Mx gene 3' sequence in Langya chicken breeds of Shandong Province. 展开更多
关键词 Langya chicken mxgene PCR-RFLP Sequence analysis
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北京鸭Mx基因克隆及其在鸭呼肠孤病毒感染的鸭胚成纤维细胞中的表达分析 被引量:1
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作者 丁明洋 陈宗艳 +1 位作者 吴润 刘光清 《中国畜牧兽医》 CAS 北大核心 2016年第1期23-30,共8页
本试验旨在研究北京鸭Mx基因的结构及功能。利用干扰素诱导剂Poly(I∶C)诱导鸭胚成纤维细胞(DEF),提取细胞总RNA,采用RT-PCR方法扩增北京鸭Mx基因全长编码区序列,并观察其在感染了鸭呼肠孤病毒(DRV)的DEF中的动态表达情况。北京... 本试验旨在研究北京鸭Mx基因的结构及功能。利用干扰素诱导剂Poly(I∶C)诱导鸭胚成纤维细胞(DEF),提取细胞总RNA,采用RT-PCR方法扩增北京鸭Mx基因全长编码区序列,并观察其在感染了鸭呼肠孤病毒(DRV)的DEF中的动态表达情况。北京鸭Mx基因序列分析结果显示,该基因编码区全长2 166bp,编码721个氨基酸残基。通过与GenBank中已登录的脊椎动物Mx基因进行核苷酸系统进化树分析,结果显示北京鸭Mx基因与野鸭Mx基因关系最近。北京鸭Mx序列与其他动物基因序列的比对结果显示,核苷酸同源性为47.8%~99.8%,氨基酸同源性为47.9%~99.4%。DRV孵育DEF后,Mx呈波动性表达。结果表明,本试验成功克隆了北京鸭Mx基因,预测分析证实其编码的蛋白具有脊椎动物Mx蛋白共有的结构特征,北京鸭Mx蛋白全基因的获得为下一步研究禽类Mx蛋白的抗病毒活性、作用机制及干扰素的监测奠定了基础。 展开更多
关键词 MX基因 北京鸭 抗病毒活性 鸭呼肠孤病毒
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