Anthocyanins play crucial roles in pollen protection and pollinator attraction in flowering plants.However,the mechanisms underlying flower color determination and whether floral anthocyanin regulators participate in ...Anthocyanins play crucial roles in pollen protection and pollinator attraction in flowering plants.However,the mechanisms underlying flower color determination and whether floral anthocyanin regulators participate in other processes remain largely unresolved in soybeans(Glycine max).In this study,we investigated the genetic components and mechanisms governing anthocyanin biosynthesis in soybean flowers.Molecular and genetic studies have characterized two antagonistic regulators,the positive activator GmMYBA3 and the negative repressor GmMYBR1,that modulate the gene expression of anthocyanin biosynthesis in soybean flowers.Further findings revealed a regulatory interplay between GmMYBA3 and GmMYBR1 bridged by GmTT8a,highlighting the complexity of anthocyanin regulation in different soybean organs.Exploration of additional soybean cultivars demonstrated the universality of GmMYBA3 and GmMYBR1 in regulating floral anthocyanin biosynthesis-related genes,with GmF3’5’H identified as a crucial determinant of white flower color.This study provides a molecular mechanism underlying soybean flower color determination,paving the way for the molecular modification of soybean flowers to probably enhance their resistance to abiotic stresses and attractiveness to pollinators.展开更多
Anthocyanins,ubiquitous in the Compositae family,are regulated by MYB(v-myb avian myeloblastosis viral oncogene homolog),playing an important role in anthocyanin synthesis.In this study,we analyzed the regulation path...Anthocyanins,ubiquitous in the Compositae family,are regulated by MYB(v-myb avian myeloblastosis viral oncogene homolog),playing an important role in anthocyanin synthesis.In this study,we analyzed the regulation pathway in which the MYB protein of subgroup 6 promotes dihydroflavonol reductase(DFR)expression in Compositae,and validated this law in Saussurea medusa through yeast one-hybrid experiments.Our results showed that MYB and DFR underwent purification selection,DFR promoter analysis revealed the presence of MYB binding site(GAGTTGAATGG)and bHLH binding site(CANNTG)at the sense strand of 84–116 nucleotide residues from the start codon.These two motifs were separated by 9–10 nucleotide residues,as existed in the DFR promoters of many Compositae plants.Furthermore,the yeast one-hybrid experiment demonstrated that SmMYB1 can activate the promoter of SmDFR.Our results provide a reference for further functional study of DFR in Compositae.展开更多
基金supported by the National Natural Science Foundation of China(32201781,32100211)the Department of Science and Technology of Jilin Province(20220508112RC,20210101005JC)+1 种基金the Fundamental Research Fund for the Central Universities(2412023YQ005)China Agriculture Research System(CARS04)。
文摘Anthocyanins play crucial roles in pollen protection and pollinator attraction in flowering plants.However,the mechanisms underlying flower color determination and whether floral anthocyanin regulators participate in other processes remain largely unresolved in soybeans(Glycine max).In this study,we investigated the genetic components and mechanisms governing anthocyanin biosynthesis in soybean flowers.Molecular and genetic studies have characterized two antagonistic regulators,the positive activator GmMYBA3 and the negative repressor GmMYBR1,that modulate the gene expression of anthocyanin biosynthesis in soybean flowers.Further findings revealed a regulatory interplay between GmMYBA3 and GmMYBR1 bridged by GmTT8a,highlighting the complexity of anthocyanin regulation in different soybean organs.Exploration of additional soybean cultivars demonstrated the universality of GmMYBA3 and GmMYBR1 in regulating floral anthocyanin biosynthesis-related genes,with GmF3’5’H identified as a crucial determinant of white flower color.This study provides a molecular mechanism underlying soybean flower color determination,paving the way for the molecular modification of soybean flowers to probably enhance their resistance to abiotic stresses and attractiveness to pollinators.
基金This work was financially supported by the National Natural Science Foundation of China(31960222,31360095).
文摘Anthocyanins,ubiquitous in the Compositae family,are regulated by MYB(v-myb avian myeloblastosis viral oncogene homolog),playing an important role in anthocyanin synthesis.In this study,we analyzed the regulation pathway in which the MYB protein of subgroup 6 promotes dihydroflavonol reductase(DFR)expression in Compositae,and validated this law in Saussurea medusa through yeast one-hybrid experiments.Our results showed that MYB and DFR underwent purification selection,DFR promoter analysis revealed the presence of MYB binding site(GAGTTGAATGG)and bHLH binding site(CANNTG)at the sense strand of 84–116 nucleotide residues from the start codon.These two motifs were separated by 9–10 nucleotide residues,as existed in the DFR promoters of many Compositae plants.Furthermore,the yeast one-hybrid experiment demonstrated that SmMYB1 can activate the promoter of SmDFR.Our results provide a reference for further functional study of DFR in Compositae.