Three soil-transmitted pathogenic fungi including Rhizoctonia solani, Fusarium solani, and Pythium sp. were selected to investigate the effect of lanthanum on their growth and the pathogenic enzymes using liquid cultu...Three soil-transmitted pathogenic fungi including Rhizoctonia solani, Fusarium solani, and Pythium sp. were selected to investigate the effect of lanthanum on their growth and the pathogenic enzymes using liquid culture. Variance analysis shows significant differences among treatments with different concentrations of lanthanum （Rhizoctonia solani F = 6.75 〉 F0.01= 5.99; Fusarium solani F = 18.1 〉 F0.01 = 5.99, Pythium sp. F = 23.29 〉 F0.01 = 5.99）. The inhibitory effect of lanthanum on pathogenic fungi increased with an increase in La concentration. The activities of the three pathogenic enzymes per gram mycelium were promoted remarkably. However, the quantity or the activities of the total enzymes were inhibited because of the strong inhibition of mycelium growth by lanthanum. Meanwhile, the effect of lanthanum on toxins of pathogenic fungi were studied using the seed germination experiment. Toxins of pathogenic fungi are influenced by lanthanum and the virulence decreases significantly with the increase of lanthanum concentration.展开更多
Mycelial growth and yield of two strains ofA. aegerita on different substrates was investigated. Mycelial growth on agar media （PDA, standard, wheat, MEA, CYM, PCA, sawdust extracts： alder and mixture of beech and b...Mycelial growth and yield of two strains ofA. aegerita on different substrates was investigated. Mycelial growth on agar media （PDA, standard, wheat, MEA, CYM, PCA, sawdust extracts： alder and mixture of beech and birch （1：1）） and sawdust substrates （birch, beech, oak, maple, alder） was investigated. Petri dishes （Ф 9 cm） for agar media and biological tubes （18 cm long and Ф 2.5 cm） for sawdust substrates were used. Yield and morphological features were studied on birch, beech and mixture of beech and alder （1：1） sawdust substrates. The temperature of inoculation for agar media and sawdust substrates was 25 ℃. For yielding, when mycelium has completely overgrown the substrate the temperature was decreased to 15-17 ℃ to initiate primordia formation. The cultivation was enlightened 10 h/d with daylight lamps （500 Ix）. One crop was harvested after five weeks. The carpophores of black poplar mushrooms were picked up in clusters. There was no statistically important difference between the mycelial growths of the investigated strains. The best growing agar media were PDA （7.3 cm）, MEA （7.2 cm） and wheat （7.1 cm）, both strains showed the slowest mycelium growth on CYM （5.2 cm）. The mycelial growth on sawdust was the best on the beech （7.1 cm） and birch （6.8 cm） sawdust. The best substrate for cultivation ofA. aegerita was mixture of beech and alder sawdust （39.5 g/100 substrate DM）. The dry yield of carpophores were the highest on beech and alder substrate （3.2 g/100 substrate DM）, but dry matter content was the highest on beech sawdust （15.7%）. The heaviest carpophores were harvested from birch sawdust （3.7 g）; the biggest caps from beech and alder sawdust （3.3 cm）. There were no statistically important differences between the mycelium growth and yielding between both investigated strains.展开更多
[ Objective ] The paper was to study the effect of sea brine on the growth of Fusarium graminearum. [ Method] The inhibition rate of sea brine against F. gram/nearum was measured using mycelial growth rate method. The...[ Objective ] The paper was to study the effect of sea brine on the growth of Fusarium graminearum. [ Method] The inhibition rate of sea brine against F. gram/nearum was measured using mycelial growth rate method. The inhibition effect of sea brine against infection of F. gram/nearum on maize was measured through leaf culture method in vitro. [Result] With the decrease of sea brine concentration, its inhibition against F. gram/nearum had no remarkable regulation, which first decreased, then increased, and finally decreased. It had the best inhibition effect as the concentration was 0.005 0% with the inhibition rate of 31.2%. 0.050 0% sea brine had the best inhibition effect against the infection of F. graminearum with the inhibition rate of 44.3%. [ Conclusion] The results provided theoretical basis for the application of sea brine in the aspects of plant diseases and vests control展开更多
Effects of extract of medium residue of Pleurotus citrinopileatus on mycelial growth of P. citrinopileatus, G. lucidum and L. edodes were investigated by plate cultivation method. The results showed that extract of me...Effects of extract of medium residue of Pleurotus citrinopileatus on mycelial growth of P. citrinopileatus, G. lucidum and L. edodes were investigated by plate cultivation method. The results showed that extract of medium residue of P. citrinopileatus promoted mycelial growth of L. edodes, and showed the best effects at the addition amount of 75%, while the myedial growth of G. lucidum and P. citrinopileatus was inhibited by the extract.展开更多
基金Project supported by Research Promotion Foundation of China Agricultural University and Sino-American Turf ManagementProject
文摘Three soil-transmitted pathogenic fungi including Rhizoctonia solani, Fusarium solani, and Pythium sp. were selected to investigate the effect of lanthanum on their growth and the pathogenic enzymes using liquid culture. Variance analysis shows significant differences among treatments with different concentrations of lanthanum （Rhizoctonia solani F = 6.75 〉 F0.01= 5.99; Fusarium solani F = 18.1 〉 F0.01 = 5.99, Pythium sp. F = 23.29 〉 F0.01 = 5.99）. The inhibitory effect of lanthanum on pathogenic fungi increased with an increase in La concentration. The activities of the three pathogenic enzymes per gram mycelium were promoted remarkably. However, the quantity or the activities of the total enzymes were inhibited because of the strong inhibition of mycelium growth by lanthanum. Meanwhile, the effect of lanthanum on toxins of pathogenic fungi were studied using the seed germination experiment. Toxins of pathogenic fungi are influenced by lanthanum and the virulence decreases significantly with the increase of lanthanum concentration.
文摘Mycelial growth and yield of two strains ofA. aegerita on different substrates was investigated. Mycelial growth on agar media （PDA, standard, wheat, MEA, CYM, PCA, sawdust extracts： alder and mixture of beech and birch （1：1）） and sawdust substrates （birch, beech, oak, maple, alder） was investigated. Petri dishes （Ф 9 cm） for agar media and biological tubes （18 cm long and Ф 2.5 cm） for sawdust substrates were used. Yield and morphological features were studied on birch, beech and mixture of beech and alder （1：1） sawdust substrates. The temperature of inoculation for agar media and sawdust substrates was 25 ℃. For yielding, when mycelium has completely overgrown the substrate the temperature was decreased to 15-17 ℃ to initiate primordia formation. The cultivation was enlightened 10 h/d with daylight lamps （500 Ix）. One crop was harvested after five weeks. The carpophores of black poplar mushrooms were picked up in clusters. There was no statistically important difference between the mycelial growths of the investigated strains. The best growing agar media were PDA （7.3 cm）, MEA （7.2 cm） and wheat （7.1 cm）, both strains showed the slowest mycelium growth on CYM （5.2 cm）. The mycelial growth on sawdust was the best on the beech （7.1 cm） and birch （6.8 cm） sawdust. The best substrate for cultivation ofA. aegerita was mixture of beech and alder sawdust （39.5 g/100 substrate DM）. The dry yield of carpophores were the highest on beech and alder substrate （3.2 g/100 substrate DM）, but dry matter content was the highest on beech sawdust （15.7%）. The heaviest carpophores were harvested from birch sawdust （3.7 g）; the biggest caps from beech and alder sawdust （3.3 cm）. There were no statistically important differences between the mycelium growth and yielding between both investigated strains.
基金Supported by Key Projects in Social Development Field of Guangdong Province,Science and Technology Department of Guangdong Province "Green Using Technology of Waste Brine"(A2009011-007(c))~~
文摘[ Objective ] The paper was to study the effect of sea brine on the growth of Fusarium graminearum. [ Method] The inhibition rate of sea brine against F. gram/nearum was measured using mycelial growth rate method. The inhibition effect of sea brine against infection of F. gram/nearum on maize was measured through leaf culture method in vitro. [Result] With the decrease of sea brine concentration, its inhibition against F. gram/nearum had no remarkable regulation, which first decreased, then increased, and finally decreased. It had the best inhibition effect as the concentration was 0.005 0% with the inhibition rate of 31.2%. 0.050 0% sea brine had the best inhibition effect against the infection of F. graminearum with the inhibition rate of 44.3%. [ Conclusion] The results provided theoretical basis for the application of sea brine in the aspects of plant diseases and vests control
基金Supported by Project of Mudanjiang Normal University for Serving the Local(FD2014003)Science and Technology Research Project of Department of Education,Helongjiang Province(12531721)College Students' Innovation and Enterpreneurship Training Program of Helongjiang Province(201610233029)
文摘Effects of extract of medium residue of Pleurotus citrinopileatus on mycelial growth of P. citrinopileatus, G. lucidum and L. edodes were investigated by plate cultivation method. The results showed that extract of medium residue of P. citrinopileatus promoted mycelial growth of L. edodes, and showed the best effects at the addition amount of 75%, while the myedial growth of G. lucidum and P. citrinopileatus was inhibited by the extract.
