Objective:To isolate and identify the exact species of the genus Mycobacterium from Didelphis(D.)virginiana,and the direct implications of this bacterium to public health and veterinary medicine.Methods:Thirty-one D.v...Objective:To isolate and identify the exact species of the genus Mycobacterium from Didelphis(D.)virginiana,and the direct implications of this bacterium to public health and veterinary medicine.Methods:Thirty-one D.virginiana were captured and necropsied in Hidalgo,Mexico.Tissue samples were collected to culture mycobacteria present and examine individual specimens’histopathology.Mycobacterium identification was obtained through the application of amplification and sequencing of 16S rDNA techniques.Results:Three strains were isolated and identified as Mycobacterium(M.)avium subsp.hominissuis by utilizing M.avium complexspecific primers.Granulomatous lesions were observed in the subpleural zone(granuloma gradeⅡ)and bronchial(granuloma gradeⅠ)of the lungs of D.virginiana with positive isolation.Conclusions:Three strains of M.avium subsp.hominissuis,from lung tissue samples of D.virginiana were identified.This subspecies of M.avium has important implications in public health and veterinary medicine.展开更多
Johne's disease or paratuberculosis is a chronic mycobacterial infection that affects cattle, sheep, goats and other ruminants, adversely, leading to huge economic losses throughout the world. The estimation of se...Johne's disease or paratuberculosis is a chronic mycobacterial infection that affects cattle, sheep, goats and other ruminants, adversely, leading to huge economic losses throughout the world. The estimation of sero-prevalence of this disease in the cattle population of south-western Bangalore, Karnataka, using an immunological assay and statistical analyses, was the objective of this study. One of the diagnostic tools used to detect an antigen or an antibody in animal serum or milk is the Enzyme Linked Immuno-Sorbent Assay, which has been widely used in the research and diagnosis of animal and human diseases as its accuracy is of nanogram-picogram/milliltre level. In the present study, indirect-ELISA was used to diagnose and estimate the sero-prevalence of paratuberculosis in cattle showing diarrhoea and/or anaemia, at 5 local dairy farms in south-west Bangalore, India. Out of 350 bovine serum samples, 53 (15.14%) were positive, 55 milk samples out of 300 were found positive (18.33%) for antibody against Johne's disease by indirect ELISA. The positive samples were then confirmed by direct smear examination of dung by Ziehl-Neelsen staining. Statistical analyses were carried out to indicate the seroprevalence of Johne's disease in the cattle population of this region to be 15 ± 10%, taking a confidence interval of 95%. The results emphasize the need to prevent the further spread of infection to other susceptible animals and humans as the causative organism, Mycobacterium avium subsp. paratuberculosis is implicated in Crohn's disease, an irritable bowel syndrome in humans.展开更多
Paratuberculosis is a chronic wasting disease of granulomatous enteritis in ruminants caused by Mycobacterium avium subsp.paratuberculosis(M.paratuberculosis,MAP)resulting in heavy economic losses to dairy industries ...Paratuberculosis is a chronic wasting disease of granulomatous enteritis in ruminants caused by Mycobacterium avium subsp.paratuberculosis(M.paratuberculosis,MAP)resulting in heavy economic losses to dairy industries worldwide.Currently,commercial vaccines were not effective in preventing pathogen shedding and were reported with serious side effects.To develop a novel and smarter paratuberculosis vaccine,we utilized PLGA nanoparticles encapsulating the Bfra antigen(Bfra-PLGA NPs).We observed that mice vaccinated with Bfra-PLGA NPs exhibited an enhanced secretory IFN-γ,CD4+T cells response and antibody IgG against MAP infection.In addition,secretions of the inflammatory cytokine TNF-αand IL-10 were increased following treatment with Bfra-PLGA NPs.A significant reduction in bacterial load was observed in the livers and spleens of animals vaccinated with Bfra-PLGA NPs.Furthermore,Bfra-PLGA NPs were effective to alleviate the pathological lesions of livers in mice.Overall,our approach provides a rational basis for employing PLGA nanoparticles to develop improved vaccines that induced protective immunity against paratuberculosis.展开更多
基金the financial assistance from the Secretary of Research of Universidad Autónoma del Estado de Hidalgo(UAEH)through the project“Epidemiological surveillance of the genus Mycobacterium in the species Didelphis virginiana(Tlacuache)of the valley of Tulancingo Hidalgo”with number UAEH/DI/ICAp/MVZ/6.
文摘Objective:To isolate and identify the exact species of the genus Mycobacterium from Didelphis(D.)virginiana,and the direct implications of this bacterium to public health and veterinary medicine.Methods:Thirty-one D.virginiana were captured and necropsied in Hidalgo,Mexico.Tissue samples were collected to culture mycobacteria present and examine individual specimens’histopathology.Mycobacterium identification was obtained through the application of amplification and sequencing of 16S rDNA techniques.Results:Three strains were isolated and identified as Mycobacterium(M.)avium subsp.hominissuis by utilizing M.avium complexspecific primers.Granulomatous lesions were observed in the subpleural zone(granuloma gradeⅡ)and bronchial(granuloma gradeⅠ)of the lungs of D.virginiana with positive isolation.Conclusions:Three strains of M.avium subsp.hominissuis,from lung tissue samples of D.virginiana were identified.This subspecies of M.avium has important implications in public health and veterinary medicine.
文摘Johne's disease or paratuberculosis is a chronic mycobacterial infection that affects cattle, sheep, goats and other ruminants, adversely, leading to huge economic losses throughout the world. The estimation of sero-prevalence of this disease in the cattle population of south-western Bangalore, Karnataka, using an immunological assay and statistical analyses, was the objective of this study. One of the diagnostic tools used to detect an antigen or an antibody in animal serum or milk is the Enzyme Linked Immuno-Sorbent Assay, which has been widely used in the research and diagnosis of animal and human diseases as its accuracy is of nanogram-picogram/milliltre level. In the present study, indirect-ELISA was used to diagnose and estimate the sero-prevalence of paratuberculosis in cattle showing diarrhoea and/or anaemia, at 5 local dairy farms in south-west Bangalore, India. Out of 350 bovine serum samples, 53 (15.14%) were positive, 55 milk samples out of 300 were found positive (18.33%) for antibody against Johne's disease by indirect ELISA. The positive samples were then confirmed by direct smear examination of dung by Ziehl-Neelsen staining. Statistical analyses were carried out to indicate the seroprevalence of Johne's disease in the cattle population of this region to be 15 ± 10%, taking a confidence interval of 95%. The results emphasize the need to prevent the further spread of infection to other susceptible animals and humans as the causative organism, Mycobacterium avium subsp. paratuberculosis is implicated in Crohn's disease, an irritable bowel syndrome in humans.
基金supported by"National Key Research and Development Program(Project No.2021YFD1800405)""National Natural Science Foundation of China(Project No.31873005,No.32172800)""China Agriculture Research System(No.CARS-36)".
文摘Paratuberculosis is a chronic wasting disease of granulomatous enteritis in ruminants caused by Mycobacterium avium subsp.paratuberculosis(M.paratuberculosis,MAP)resulting in heavy economic losses to dairy industries worldwide.Currently,commercial vaccines were not effective in preventing pathogen shedding and were reported with serious side effects.To develop a novel and smarter paratuberculosis vaccine,we utilized PLGA nanoparticles encapsulating the Bfra antigen(Bfra-PLGA NPs).We observed that mice vaccinated with Bfra-PLGA NPs exhibited an enhanced secretory IFN-γ,CD4+T cells response and antibody IgG against MAP infection.In addition,secretions of the inflammatory cytokine TNF-αand IL-10 were increased following treatment with Bfra-PLGA NPs.A significant reduction in bacterial load was observed in the livers and spleens of animals vaccinated with Bfra-PLGA NPs.Furthermore,Bfra-PLGA NPs were effective to alleviate the pathological lesions of livers in mice.Overall,our approach provides a rational basis for employing PLGA nanoparticles to develop improved vaccines that induced protective immunity against paratuberculosis.
