Although it has been known that γδ T cells may play an important role in the immune response to infection of Mycobacterium tuberculosis （M. tb）, the mechanisms by which the γδ T cells participate in the innate a...Although it has been known that γδ T cells may play an important role in the immune response to infection of Mycobacterium tuberculosis （M. tb）, the mechanisms by which the γδ T cells participate in the innate and/or acquired immunity to tuberculosis （TB） have not been full elucidated. In the present study, 27 patients with active pulmonary TB and 16 healthy donors （HD） were performed. We found that proportion of IL-17-producing cells among lymphocyte was similar between TB patients and HD, whereas the proportions of γδ T cells in IL-17-producing cells （59.2%） and IL-17-producing cells in γδ T cells （19.4%） in peripheral blood were markedly increased in TB patients when compared to those in HD （43.9% and 7.7%, respectively）. In addition, the proportions of IFN-T-producing γδ T cells in TB patients were obviously lower than that in HD. Upon re-stimulated with M. tb heat-treated antigen （M. tb-HAg） in vitro, fewer IL-17-producing γδ T cells were generated from HD and TB patients, whereas IFN-T-producing γδ T cells were increased in TB patients compared to that in HD. Our findings in TB patients and healthy human were consistent with other murine investigation that the IL-17- producing γδ T cells were main source of IL-17 in mouse model of BCG infection, suggesting that γδ T cells might be involved in the formation of tubercular granuloma in pulmonary TB patients, but need further identification.展开更多
目的探讨初诊肺结核患者血浆白细胞介素-6(IL-6)、白细胞介素-17(IL-17)、白细胞介素-37(IL-37)及T细胞免疫球蛋白黏蛋白分子-3(TIM-3)水平变化及其临床意义。方法收集2018年1月~2020年10月百色市人民医院收治的活动性肺结核患者57例、...目的探讨初诊肺结核患者血浆白细胞介素-6(IL-6)、白细胞介素-17(IL-17)、白细胞介素-37(IL-37)及T细胞免疫球蛋白黏蛋白分子-3(TIM-3)水平变化及其临床意义。方法收集2018年1月~2020年10月百色市人民医院收治的活动性肺结核患者57例、肺炎患者50例和健康对照40例。57例肺结核患者中菌阳肺结核31例,菌阴肺结核26例。57例肺结核按病情轻重、病程长短及病变范围分为轻症组32例和重症组25例。采用酶联免疫吸附测定(ELISA)法检测各组血浆IL-6,IL-17,IL-37及TIM-3水平,并进行比较。结果肺结核组血浆IL-6(41.37±13.50 pg/ml vs 26.28±9.16pg/ml,3.05±1.08 pg/ml),IL-17(62.50±10.73pg/ml vs 30.47±7.18pg/ml,16.13±5.86pg/ml),IL-37(14.63±4.18pg/ml vs 9.85±2.74pg/ml,4.10±1.02 pg/ml)及TIM-3(18.17±5.16ng/ml vs 11.80±3.52ng/ml,6.24±2.15 ng/ml)水平均明显高于肺炎组和对照组,差异均有统计学意义(t=7.338~13.273,均P<0.001)。菌阳肺结核组血浆IL-6(52.60±15.71pg/ml vs 30.16±8.95 pg/ml),IL-17(72.35±15.20 pg/ml vs 46.52±9.13 pg/ml),IL-37(16.50±6.14 pg/ml vs 12.48±3.17 pg/ml)及TIM-3(21.70±7.93ng/ml vs 15.21±4.92 ng/ml)水平均明显高于菌阴肺结核组,差异具有统计学意义(t=8.472,10.161,6.925,9.106,均P<0.001)。重症组血浆IL-6(56.38±17.26pg/ml vs 25.84±9.27pg/ml),IL-17(79.50±16.38 pg/ml vs 48.20±8.74 pg/ml),IL-37(18.48±6.20 pg/ml vs 10.82±3.26pg/ml)及TIM-3(23.26±8.15ng/ml vs 13.90±4.71ng/ml)水平明显高于轻症组,差异具有统计学意义(t=12.642,14.513,10.205,13.172,均P<0.001)。结论血浆IL-6,IL-17,IL-37及TIM-3水平在肺结核患者中明显升高,对判断肺结核严重程度有一定的价值。展开更多
文摘Although it has been known that γδ T cells may play an important role in the immune response to infection of Mycobacterium tuberculosis （M. tb）, the mechanisms by which the γδ T cells participate in the innate and/or acquired immunity to tuberculosis （TB） have not been full elucidated. In the present study, 27 patients with active pulmonary TB and 16 healthy donors （HD） were performed. We found that proportion of IL-17-producing cells among lymphocyte was similar between TB patients and HD, whereas the proportions of γδ T cells in IL-17-producing cells （59.2%） and IL-17-producing cells in γδ T cells （19.4%） in peripheral blood were markedly increased in TB patients when compared to those in HD （43.9% and 7.7%, respectively）. In addition, the proportions of IFN-T-producing γδ T cells in TB patients were obviously lower than that in HD. Upon re-stimulated with M. tb heat-treated antigen （M. tb-HAg） in vitro, fewer IL-17-producing γδ T cells were generated from HD and TB patients, whereas IFN-T-producing γδ T cells were increased in TB patients compared to that in HD. Our findings in TB patients and healthy human were consistent with other murine investigation that the IL-17- producing γδ T cells were main source of IL-17 in mouse model of BCG infection, suggesting that γδ T cells might be involved in the formation of tubercular granuloma in pulmonary TB patients, but need further identification.
文摘目的探讨初诊肺结核患者血浆白细胞介素-6(IL-6)、白细胞介素-17(IL-17)、白细胞介素-37(IL-37)及T细胞免疫球蛋白黏蛋白分子-3(TIM-3)水平变化及其临床意义。方法收集2018年1月~2020年10月百色市人民医院收治的活动性肺结核患者57例、肺炎患者50例和健康对照40例。57例肺结核患者中菌阳肺结核31例,菌阴肺结核26例。57例肺结核按病情轻重、病程长短及病变范围分为轻症组32例和重症组25例。采用酶联免疫吸附测定(ELISA)法检测各组血浆IL-6,IL-17,IL-37及TIM-3水平,并进行比较。结果肺结核组血浆IL-6(41.37±13.50 pg/ml vs 26.28±9.16pg/ml,3.05±1.08 pg/ml),IL-17(62.50±10.73pg/ml vs 30.47±7.18pg/ml,16.13±5.86pg/ml),IL-37(14.63±4.18pg/ml vs 9.85±2.74pg/ml,4.10±1.02 pg/ml)及TIM-3(18.17±5.16ng/ml vs 11.80±3.52ng/ml,6.24±2.15 ng/ml)水平均明显高于肺炎组和对照组,差异均有统计学意义(t=7.338~13.273,均P<0.001)。菌阳肺结核组血浆IL-6(52.60±15.71pg/ml vs 30.16±8.95 pg/ml),IL-17(72.35±15.20 pg/ml vs 46.52±9.13 pg/ml),IL-37(16.50±6.14 pg/ml vs 12.48±3.17 pg/ml)及TIM-3(21.70±7.93ng/ml vs 15.21±4.92 ng/ml)水平均明显高于菌阴肺结核组,差异具有统计学意义(t=8.472,10.161,6.925,9.106,均P<0.001)。重症组血浆IL-6(56.38±17.26pg/ml vs 25.84±9.27pg/ml),IL-17(79.50±16.38 pg/ml vs 48.20±8.74 pg/ml),IL-37(18.48±6.20 pg/ml vs 10.82±3.26pg/ml)及TIM-3(23.26±8.15ng/ml vs 13.90±4.71ng/ml)水平明显高于轻症组,差异具有统计学意义(t=12.642,14.513,10.205,13.172,均P<0.001)。结论血浆IL-6,IL-17,IL-37及TIM-3水平在肺结核患者中明显升高,对判断肺结核严重程度有一定的价值。
