期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
A novel recombinant DNA vaccine encodingMycobacterium tuberculosis ESAT-6 and FL protects againstMycobacterium tuberculosis challenge in mice 被引量:3
1
作者 Qingtao Jiang Jing Zhang +9 位作者 Xia Chen Mei Xia Yanlai Lu Wen Qiu Ganzhu Feng Dan Zhao Yan Li Fengxia He Guangyong Peng Yingwei Wang 《The Journal of Biomedical Research》 CAS 2013年第5期406-420,共15页
Mycobacterium tuberculosis 6-kDa early secretory antigenic target (ESAT-6) is a dominant target antigen for cell-mediated immunity in the early phase of tuberculosis. The fms-like tyrosine kinase 3 ligand (FL) tha... Mycobacterium tuberculosis 6-kDa early secretory antigenic target (ESAT-6) is a dominant target antigen for cell-mediated immunity in the early phase of tuberculosis. The fms-like tyrosine kinase 3 ligand (FL) that induces potent immune response has been used as an adjuvant in vaccine development. In this study, a new recombinant plasmid (plRES-epitope-peptides-FL) encoding three T cell epitopes of ESAT-6 and FL was constructed, and the immunogenicity of the DNA vaccine was assessed in C57BL/6 mice immunized with the plasmid DNA vaccine. Additionally, a strategy of intramuscular injection with the DNA vaccine (prime) and intranasal administration of the epitope peptides (boost) was employed to induce higher immune reaction of the mice. The results showed that mice vaccinated with the recombinant plasmid DNA vaccine and boosted with the peptides not only increased the levels of Thl cytokines (IFN-γ and IL-12), the number of IFN-γ+ T cells and activities of cytotoxic T lymphocytes as well as IgG, but also enhanced protection against Mycobacterium tuberculosis challenge. In conclusion, these data indicate that the novel recombinant plRES-epitope-peptides-FL plasmid is a useful DNA vaccine for pre- venting Mycobacterium tuberculosis infection. 展开更多
关键词 early secretory antigenic target-6 (ESAT-6) fms-like tyrosine kinase 3 ligand (FL) mycobacteriumtuberculosis recombinant plasmid T cell epitopes
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部