This study compares the kinetics of adherence of both the prototype strain PG-18 and the recently clinically isolated strain P-140 of Mycoplasma fermentans to HEp-2 cells. M. fermentans may induce respiratory and geni...This study compares the kinetics of adherence of both the prototype strain PG-18 and the recently clinically isolated strain P-140 of Mycoplasma fermentans to HEp-2 cells. M. fermentans may induce respiratory and genitourinary tract infections. We investigated the presence of putative adhesins from strain P-140. A surface protein of 47 kDa, labeled SP47, isolated from M. fermentans P-140, seems to be involved in acterial adherence on HEp-2 cells;this study could allow helping understand the interaction between mycoplasmas and their hosts. Anti-SP47 antibodies inhibited the formation of bacterial clusters and adherence to cultured cells, quantified by ELISA. M. fermentans P-140 was more efficient at adhering to cultured HEp-2 cells than PG-18.展开更多
To explore the mechanism of the inhibition of HIV-1 by Mycoplasma fermentans, culture supernatants and thallodic proteins from M.fermentans PG18 were prepared and the protein components of the supernatants were purifi...To explore the mechanism of the inhibition of HIV-1 by Mycoplasma fermentans, culture supernatants and thallodic proteins from M.fermentans PG18 were prepared and the protein components of the supernatants were purified with high performance liquid chromatography (HPLC).The inhibitory activities to reverse transcriptase (RT) and the nuclease activities were detected; the influence of M.fermentans on IL-10 secretion by both normal and HIV-1 infected human PBMC were determined, and the inhibitory effect of rhIL-10 on HIV-1 replication was detected with ELISA method. The results showed that the purified proteins with a molecular weight of 67-100 kDa or 10-25 kDa showed a 36% or 34% in hibitory activity to RT and partial nuclease activity. The thallodic protein could induce both normal and HIV-1 infected PBMC to secret IL-10 remarkably, and to the latter, this effect was more apparent. While rhIL-10 could inhibit replication of HIV-1 in PBMC in vitro in a dose-dependant manner. It concludes that the inhibitory effect of the M.fermentans PG18 culture supernatants on RT and the promoting effect of PG18 thallodic protein on IL-10 secretion in PBMC explain the mechanisms of inhibition to HIV-1 by M.fermentans PG18.展开更多
基金M.Rosales-Pérez was supported by a scholarship No.91996 from Consejo Nacional de Ciencia y Tecnología(CONACYT)México.
文摘This study compares the kinetics of adherence of both the prototype strain PG-18 and the recently clinically isolated strain P-140 of Mycoplasma fermentans to HEp-2 cells. M. fermentans may induce respiratory and genitourinary tract infections. We investigated the presence of putative adhesins from strain P-140. A surface protein of 47 kDa, labeled SP47, isolated from M. fermentans P-140, seems to be involved in acterial adherence on HEp-2 cells;this study could allow helping understand the interaction between mycoplasmas and their hosts. Anti-SP47 antibodies inhibited the formation of bacterial clusters and adherence to cultured cells, quantified by ELISA. M. fermentans P-140 was more efficient at adhering to cultured HEp-2 cells than PG-18.
基金This study was supported by a grant from the National Natural Science Foundation of China (No. 39770678)
文摘To explore the mechanism of the inhibition of HIV-1 by Mycoplasma fermentans, culture supernatants and thallodic proteins from M.fermentans PG18 were prepared and the protein components of the supernatants were purified with high performance liquid chromatography (HPLC).The inhibitory activities to reverse transcriptase (RT) and the nuclease activities were detected; the influence of M.fermentans on IL-10 secretion by both normal and HIV-1 infected human PBMC were determined, and the inhibitory effect of rhIL-10 on HIV-1 replication was detected with ELISA method. The results showed that the purified proteins with a molecular weight of 67-100 kDa or 10-25 kDa showed a 36% or 34% in hibitory activity to RT and partial nuclease activity. The thallodic protein could induce both normal and HIV-1 infected PBMC to secret IL-10 remarkably, and to the latter, this effect was more apparent. While rhIL-10 could inhibit replication of HIV-1 in PBMC in vitro in a dose-dependant manner. It concludes that the inhibitory effect of the M.fermentans PG18 culture supernatants on RT and the promoting effect of PG18 thallodic protein on IL-10 secretion in PBMC explain the mechanisms of inhibition to HIV-1 by M.fermentans PG18.
