This pilot study reports the vertical transmission and reverse thermosensitivity of the MS-H vaccine strain of Mycoplasma synoviae (MS) by RAPD in commercial breeders and their progeny. At two weeks of age, breeders w...This pilot study reports the vertical transmission and reverse thermosensitivity of the MS-H vaccine strain of Mycoplasma synoviae (MS) by RAPD in commercial breeders and their progeny. At two weeks of age, breeders were vaccinated with the ts<sup>+</sup> MS-H strain. At 9 weeks of age, an outbreak of infectious synovitis (IS) was detected in the progeny. Tracheal swab samples were collected from breeders at 24, 39, 48, and 70 weeks of age. At 9 weeks, pullets swab from the elbow joints were collected. RAPD was performed on the isolates at 39.5°C, and the same ts<sup>-</sup> MS-H strains were identified in the breeder hens and their progeny. Tracheal swabs from breeder hens were negative to MS isolation at 37°C and 39.5°C at 24- and 39-weeks. MS isolation was recovered from tracheal swabs from 9/10 and 10/10 breeders at 48- and 70- week. At 9 weeks of age in the progeny, MS was isolated from tracheal swabs of 10/10 from non-IS pullets. MS was isolated from 9/10 joints samples. The isolates from breeder hens and their progeny showed non-significant differences in five antimycoplasmic MIC100 values;otherwise, enrofloxacin presented a significant difference in MIC100 value (p < 0.05). This investigation demonstrated the reversal of the thermosensitivity, pathogenicity, and vertical transmission of the MS-H strain. Consequently, it is crucial to contemplate the danger of reversing pathogenicity and transmission to progeny when applying the MS-H vaccine strain.展开更多
滑液囊支原体病是一种由滑液囊支原体(Mycoplasma synoviae)引起的鸡(Gallus gallus f. domestic)的急性或慢性传染病,可导致雏鸡死亡、呼吸困难、跛行、产蛋下降等,给家禽养殖业造成较大经济损失。概述了滑液囊支原体病主要诊断技术和...滑液囊支原体病是一种由滑液囊支原体(Mycoplasma synoviae)引起的鸡(Gallus gallus f. domestic)的急性或慢性传染病,可导致雏鸡死亡、呼吸困难、跛行、产蛋下降等,给家禽养殖业造成较大经济损失。概述了滑液囊支原体病主要诊断技术和防控技术的研究进展,并针对其研究进展分析其优缺点,以期为防治滑液囊支原体病提供参考。展开更多
文摘This pilot study reports the vertical transmission and reverse thermosensitivity of the MS-H vaccine strain of Mycoplasma synoviae (MS) by RAPD in commercial breeders and their progeny. At two weeks of age, breeders were vaccinated with the ts<sup>+</sup> MS-H strain. At 9 weeks of age, an outbreak of infectious synovitis (IS) was detected in the progeny. Tracheal swab samples were collected from breeders at 24, 39, 48, and 70 weeks of age. At 9 weeks, pullets swab from the elbow joints were collected. RAPD was performed on the isolates at 39.5°C, and the same ts<sup>-</sup> MS-H strains were identified in the breeder hens and their progeny. Tracheal swabs from breeder hens were negative to MS isolation at 37°C and 39.5°C at 24- and 39-weeks. MS isolation was recovered from tracheal swabs from 9/10 and 10/10 breeders at 48- and 70- week. At 9 weeks of age in the progeny, MS was isolated from tracheal swabs of 10/10 from non-IS pullets. MS was isolated from 9/10 joints samples. The isolates from breeder hens and their progeny showed non-significant differences in five antimycoplasmic MIC100 values;otherwise, enrofloxacin presented a significant difference in MIC100 value (p < 0.05). This investigation demonstrated the reversal of the thermosensitivity, pathogenicity, and vertical transmission of the MS-H strain. Consequently, it is crucial to contemplate the danger of reversing pathogenicity and transmission to progeny when applying the MS-H vaccine strain.
文摘滑液囊支原体病是一种由滑液囊支原体(Mycoplasma synoviae)引起的鸡(Gallus gallus f. domestic)的急性或慢性传染病,可导致雏鸡死亡、呼吸困难、跛行、产蛋下降等,给家禽养殖业造成较大经济损失。概述了滑液囊支原体病主要诊断技术和防控技术的研究进展,并针对其研究进展分析其优缺点,以期为防治滑液囊支原体病提供参考。
