RPMI-1640 was used as the basic culture medium and 30% calf serum was added. Using Berenil, tetracycline,dipterex,tiamulin,imidocarb,florfenicol,ethacri-dine,primaquine phosphate and other drug powder,the drug screeni...RPMI-1640 was used as the basic culture medium and 30% calf serum was added. Using Berenil, tetracycline,dipterex,tiamulin,imidocarb,florfenicol,ethacri-dine,primaquine phosphate and other drug powder,the drug screening experiment in vitro of Mycoplasma wenyoni was made under the conditions of 37 ℃, 5% CO2. The results showed that the effects of ethacridine was the best ,and that of dipterex and primaquine phosphate were next. The toxicity of dipterex was greater. Berenil, imidocarb and florfenicol were efficient.展开更多
[Objective]This study aimed to investigate the effects of Mycoplasma wenyoni on blood and semen quality of breeding bul s. [Method] Ten 10 healthy breeding bul s of Luxi cattle were randomly divided into two groups. B...[Objective]This study aimed to investigate the effects of Mycoplasma wenyoni on blood and semen quality of breeding bul s. [Method] Ten 10 healthy breeding bul s of Luxi cattle were randomly divided into two groups. Breeding bul s in experimental group were injected intravenously with M. wenyoni on Day 0 and Day 28. Effects of Mycoplasma wenyoni on breeding bul s were evaluated compre-hensively based on clinical symptoms, blood smears, blood physiological and bio-chemical properties, semen quality and scrotal changes. [Result] In addition to indi-vidual indices, the experimental group exhibited basical y normal clinical examination results without significant abnormal blood and semen quality. [Conclusion] This study suggests that M. wenyoni has low virulence and pathogenicity to breeding bul s af-ter artificial infection, leading to no significant changes in blood and semen quality.展开更多
[Objective] The research aimed to study the reproductive health of breed- ing bull after infecting Mycoplasma wenyoniL [Method] The blood and semen quality of breeding bull before and after drug treatment was studied ...[Objective] The research aimed to study the reproductive health of breed- ing bull after infecting Mycoplasma wenyoniL [Method] The blood and semen quality of breeding bull before and after drug treatment was studied by the methods of blood routine examination and breeding soundness examination （BSE）. [Result] Be- fore the treatment with primaquine phosphate, slight anaemia was seen in diseased bull and a lot of M.wenyonfi were seen on blood smears. The scrotal wall of dis- eased bull swelled, testis was softened, the semen quality was reduced, semen quality was decreased and the proportion of primary and secondary deformed sperms was increased. After drug treatment, M.wenyonfi quickly disappeared from blood and the clinical systems were gradually alleviated, so BSE of breeding bulls after one month was passed. [Conclusion] The research laid the foundation for fur- ther study on the relationship between M.wenyonfi and the reduction of bull＇s repro- ductive functions.展开更多
基金Supported by Spark Program of Ministry of Science and Technology(2012GA6200025)Science and Technology Development Plan Program in Shijiazhuang City(08150132A-3)Program of Qinhuangdao Science and Technology Bureau(200901A070)~~
文摘RPMI-1640 was used as the basic culture medium and 30% calf serum was added. Using Berenil, tetracycline,dipterex,tiamulin,imidocarb,florfenicol,ethacri-dine,primaquine phosphate and other drug powder,the drug screening experiment in vitro of Mycoplasma wenyoni was made under the conditions of 37 ℃, 5% CO2. The results showed that the effects of ethacridine was the best ,and that of dipterex and primaquine phosphate were next. The toxicity of dipterex was greater. Berenil, imidocarb and florfenicol were efficient.
基金Supported by Spark Program of the Ministry of Science and Technology of China(2012GA6200025)Science and Technology Development Project of Shijiazhuang City(08150132A-3)Project of Qinghuangdao Municipal Science and Technology Bureau(200901A070)~~
文摘[Objective]This study aimed to investigate the effects of Mycoplasma wenyoni on blood and semen quality of breeding bul s. [Method] Ten 10 healthy breeding bul s of Luxi cattle were randomly divided into two groups. Breeding bul s in experimental group were injected intravenously with M. wenyoni on Day 0 and Day 28. Effects of Mycoplasma wenyoni on breeding bul s were evaluated compre-hensively based on clinical symptoms, blood smears, blood physiological and bio-chemical properties, semen quality and scrotal changes. [Result] In addition to indi-vidual indices, the experimental group exhibited basical y normal clinical examination results without significant abnormal blood and semen quality. [Conclusion] This study suggests that M. wenyoni has low virulence and pathogenicity to breeding bul s af-ter artificial infection, leading to no significant changes in blood and semen quality.
基金Supported by Spark Program of Ministry of Science and Technology(2012GA6200025)Science and Technology Development Plan Program in Shijiazhuang City(08150132A-3)Program of Qinhuangdao Science and Technology Bureau(200901A070)~~
文摘[Objective] The research aimed to study the reproductive health of breed- ing bull after infecting Mycoplasma wenyoniL [Method] The blood and semen quality of breeding bull before and after drug treatment was studied by the methods of blood routine examination and breeding soundness examination （BSE）. [Result] Be- fore the treatment with primaquine phosphate, slight anaemia was seen in diseased bull and a lot of M.wenyonfi were seen on blood smears. The scrotal wall of dis- eased bull swelled, testis was softened, the semen quality was reduced, semen quality was decreased and the proportion of primary and secondary deformed sperms was increased. After drug treatment, M.wenyonfi quickly disappeared from blood and the clinical systems were gradually alleviated, so BSE of breeding bulls after one month was passed. [Conclusion] The research laid the foundation for fur- ther study on the relationship between M.wenyonfi and the reduction of bull＇s repro- ductive functions.
