BACKGROUND： In the perspective of traditional Chinese medicine, few studies have focused on the compound preparations though there are many investigations. The present study was undertaken to investigate the effect o...BACKGROUND： In the perspective of traditional Chinese medicine, few studies have focused on the compound preparations though there are many investigations. The present study was undertaken to investigate the effect of Zhenwu Tang Granule on chronic pressure-overloaded left ventricular hypertrophy in rats.METHODS： The study was performed at the laboratory of Guangzhou Institute of Respiratory Disease. Male SD rats were divided randomly into 3 groups： sham operation group （n=8）, operation group （n=15） and traditional Chinese medicine （TCM） group （n=15）.The model of myocardial hypertrophy was made by gradually constricting the abdominal aorta. Sixteen weeks later, cardiac ultrasonography was performed in all groups in order to ascertain post-operational left ventricular （LV） hypertrophy. And Zhenwu Tang Granule was added at a dose of 12 g/kg in the mixed feedstuff for 8 weeks in the TCM group. In the 24th week, weight, structure as well as function of the heart in each group were measured by high-frequency ultrasonography, and Masson＇s staining was performed on the cardiac muscles. Meanwhile, total collagen volume fraction （CVF-T） and non-coronary vessel collagen volume fraction （CVF-NV） were analyzed.RESULTS： There was an increase in the weight of the heart in the operation group, with the left ventricule dominated （P〈0.05）. The heart was enlarged, with diastolic interventricular septal distance （IVSd） and left ventricular posterior wall distance （LVPWd） dominated （P〈0.01）.There was a significant decrease in the cardiac function （P〈0.05）. The weight （P〈0.01） and volume of the heart decreased in the TCM group compared with the operation group, with IVSd and systolic left ventricular posterior wall dominated （P〈0.01）. And the cardiac function was improved （P〈0.05）. Significant interstitial and collagen hyperplasia was shown in the operation group based on pathological analysis, and various improvements were proved in the TCM group, i.e. there was a significant decrease in CVF-T and CVF-NV （P〈0.01） compared with the operation group; but no difference （P〉0.05） was found when compared with the pseudo-operation group.CONCLUSION： Zhenwu Tang Granule could reduce the weight and volume of the heart, improve the cardiac function, inhibit hyperplasia of collagen, and reverse myocardial hypertrophy in rats with pressure-overloaded left ventricular hypertrophy.展开更多
Objective To examine the eflects of endothelin - 1 (ET- 1) on mitogenesis and collagen synthesis in cultured cardiac libroblasts. Methods The mitogenesis and collagen synthesis in cultured cardiac fibroblasts were ass...Objective To examine the eflects of endothelin - 1 (ET- 1) on mitogenesis and collagen synthesis in cultured cardiac libroblasts. Methods The mitogenesis and collagen synthesis in cultured cardiac fibroblasts were assessed by incorporation of 3H- TdR and 3H-proline respectively. Results 10-12~10-8mol/L ET- 1 could enhance the incorporation rate of 3H- TdR and 3H-proline in an dose - dependent manner in cultured cardiac libroblasts. Conclusion ET- 1 could stimulate the mitogenesis of cultured cardiac fibroblasts and could enhance collagen synthesis by cultured cardiac libroblasts, this effect of ET- 1 is probably related to the formation of myocardial collagen network remodeling.展开更多
Objective: To study the effect of Astragalus(As) on left ventricular collagen remodeling after myocardial infarction in rats. Methods: Myocardial collagen (MC) and ratio of myocardial collagen type Ⅰ and Ⅲ in rat mo...Objective: To study the effect of Astragalus(As) on left ventricular collagen remodeling after myocardial infarction in rats. Methods: Myocardial collagen (MC) and ratio of myocardial collagen type Ⅰ and Ⅲ in rat models of myocardial infarction treated with Astragalus injection were determined and compared with those in sham operated rats and in captopril(CAP) treated rats. Results: MC and ratio of MC Ⅰ/Ⅲ of non-infarcted region in the myocardial infarction rats was higher than that in the sham group. Both AS and CAP could restrain the ascending trend of these parameters.Conclusion: Astragalus is effective in lowering the elevation of MC after infarction and changing the MC Ⅰ/Ⅲ ratio, therefore, is beneficial to improve the systolic and diastolic function of left ventricle after acute myocardial infarction展开更多
文摘BACKGROUND： In the perspective of traditional Chinese medicine, few studies have focused on the compound preparations though there are many investigations. The present study was undertaken to investigate the effect of Zhenwu Tang Granule on chronic pressure-overloaded left ventricular hypertrophy in rats.METHODS： The study was performed at the laboratory of Guangzhou Institute of Respiratory Disease. Male SD rats were divided randomly into 3 groups： sham operation group （n=8）, operation group （n=15） and traditional Chinese medicine （TCM） group （n=15）.The model of myocardial hypertrophy was made by gradually constricting the abdominal aorta. Sixteen weeks later, cardiac ultrasonography was performed in all groups in order to ascertain post-operational left ventricular （LV） hypertrophy. And Zhenwu Tang Granule was added at a dose of 12 g/kg in the mixed feedstuff for 8 weeks in the TCM group. In the 24th week, weight, structure as well as function of the heart in each group were measured by high-frequency ultrasonography, and Masson＇s staining was performed on the cardiac muscles. Meanwhile, total collagen volume fraction （CVF-T） and non-coronary vessel collagen volume fraction （CVF-NV） were analyzed.RESULTS： There was an increase in the weight of the heart in the operation group, with the left ventricule dominated （P〈0.05）. The heart was enlarged, with diastolic interventricular septal distance （IVSd） and left ventricular posterior wall distance （LVPWd） dominated （P〈0.01）.There was a significant decrease in the cardiac function （P〈0.05）. The weight （P〈0.01） and volume of the heart decreased in the TCM group compared with the operation group, with IVSd and systolic left ventricular posterior wall dominated （P〈0.01）. And the cardiac function was improved （P〈0.05）. Significant interstitial and collagen hyperplasia was shown in the operation group based on pathological analysis, and various improvements were proved in the TCM group, i.e. there was a significant decrease in CVF-T and CVF-NV （P〈0.01） compared with the operation group; but no difference （P〉0.05） was found when compared with the pseudo-operation group.CONCLUSION： Zhenwu Tang Granule could reduce the weight and volume of the heart, improve the cardiac function, inhibit hyperplasia of collagen, and reverse myocardial hypertrophy in rats with pressure-overloaded left ventricular hypertrophy.
文摘Objective To examine the eflects of endothelin - 1 (ET- 1) on mitogenesis and collagen synthesis in cultured cardiac libroblasts. Methods The mitogenesis and collagen synthesis in cultured cardiac fibroblasts were assessed by incorporation of 3H- TdR and 3H-proline respectively. Results 10-12~10-8mol/L ET- 1 could enhance the incorporation rate of 3H- TdR and 3H-proline in an dose - dependent manner in cultured cardiac libroblasts. Conclusion ET- 1 could stimulate the mitogenesis of cultured cardiac fibroblasts and could enhance collagen synthesis by cultured cardiac libroblasts, this effect of ET- 1 is probably related to the formation of myocardial collagen network remodeling.
文摘Objective: To study the effect of Astragalus(As) on left ventricular collagen remodeling after myocardial infarction in rats. Methods: Myocardial collagen (MC) and ratio of myocardial collagen type Ⅰ and Ⅲ in rat models of myocardial infarction treated with Astragalus injection were determined and compared with those in sham operated rats and in captopril(CAP) treated rats. Results: MC and ratio of MC Ⅰ/Ⅲ of non-infarcted region in the myocardial infarction rats was higher than that in the sham group. Both AS and CAP could restrain the ascending trend of these parameters.Conclusion: Astragalus is effective in lowering the elevation of MC after infarction and changing the MC Ⅰ/Ⅲ ratio, therefore, is beneficial to improve the systolic and diastolic function of left ventricle after acute myocardial infarction
