Myrcauones A-D(1-4),four new phloroglucinol-terpene adducts were isolated from the leaves of Myrciaria cauliflora.Their structures with absolute configurations were elucidated by combination of spectroscopic analysis,...Myrcauones A-D(1-4),four new phloroglucinol-terpene adducts were isolated from the leaves of Myrciaria cauliflora.Their structures with absolute configurations were elucidated by combination of spectroscopic analysis,single crystal X-ray diffraction,and electronic circular dichroism(ECD)calculations.Compound 1 was a rearranged isobutylphloroglucinol-pinene adduct featuring an unusual 2,3,4,4a,10,11-hexahydro-1H-3,11a-methanodibenzo[b,f]oxepin backbone.Compound 4 showed moderate antibacterial activity against Gram-positive bacteria including multiresistant strains.展开更多
Objective:To evaluate the antibacterial and cytotoxic effect of Myrciaria dubia(Camu camu)(M.dubia) methanol extract,against Streptococcus mutans(ATCC 25175)(S.mutans) and Streptococcus sanguinis(ATCC 10556)(S.sanguin...Objective:To evaluate the antibacterial and cytotoxic effect of Myrciaria dubia(Camu camu)(M.dubia) methanol extract,against Streptococcus mutans(ATCC 25175)(S.mutans) and Streptococcus sanguinis(ATCC 10556)(S.sanguinis).Methods:Two methanol extracts of M.dubia were prepared in vitro,from the seeds and pulp.Ten independent tests were prepared for each type of extract,using 0.12% chlorhexidine solution as positive control.Agar diffusion test was used by preparing wells with the experimental solutions cultivated in anaerobic conditions for 48 h at 37C.Meanwhile,the minimum inhibitory concentration and the cytotoxic effect over MDCK cell line was found.Results:A higher antibacterial effect was observed with the methanol seed extract with an inhibitory halo of(21.36 ± 6.35) mm and(19.21 ± 5.18) mm against S.mutans and S.sanguinis,respectively.The methanol extract of the pulp had an effect of(16.20 ± 2.08)mm and(19.34 ± 2.90) mm,respectively.The minimum inhibitory concentration of the pulp extract was 62.5 mg/mL for both strains,whereas for the seed antibacterial activity was observed even at low concentrations.The CC50 of the seeds extract was at a higher concentration than 800 mg/mL and 524.37 mg/mL for the pulp extract.Conclusions:The experimental findings demonstrated the antibacterial effect of the methanol extract of M.dubia against S.mutans and S.sanguinis.These extracts were not cytotoxic at high concentrations.展开更多
为改善3年生嘉宝果(Myrciaria cauliflora Berg)的栽培基质并促进其生长和发育,通过田间盆栽试验研究了有机碳对其实生苗地上部生长量和叶绿素含量的影响。结果表明:有机碳对嘉宝果实生苗地上部生长量和叶绿素含量均有一定的促进作用,...为改善3年生嘉宝果(Myrciaria cauliflora Berg)的栽培基质并促进其生长和发育,通过田间盆栽试验研究了有机碳对其实生苗地上部生长量和叶绿素含量的影响。结果表明:有机碳对嘉宝果实生苗地上部生长量和叶绿素含量均有一定的促进作用,以每盆150 m L有机碳效果最为显著。生产中将有机碳剂量控制在每盆100~200 m L对改良嘉宝果生长的土壤基质最为适宜,能有效促进其生长发育。展开更多
基金supported by the National Key R&D Program of China(No.2017YFC1703800)the Program for the National Natural Science Foundation of China(Nos.81822042,81630095)+2 种基金the Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(2017BT01Y036)Key-Area Research and Development Program of Guangdong Province(2020B1111110004)the High-performance Computing Platform of Jinan University.
文摘Myrcauones A-D(1-4),four new phloroglucinol-terpene adducts were isolated from the leaves of Myrciaria cauliflora.Their structures with absolute configurations were elucidated by combination of spectroscopic analysis,single crystal X-ray diffraction,and electronic circular dichroism(ECD)calculations.Compound 1 was a rearranged isobutylphloroglucinol-pinene adduct featuring an unusual 2,3,4,4a,10,11-hexahydro-1H-3,11a-methanodibenzo[b,f]oxepin backbone.Compound 4 showed moderate antibacterial activity against Gram-positive bacteria including multiresistant strains.
基金Supported by Universidad Peruana de Ciencias Aplicadas(UPC)Lima,Peru with Grant No.UPC-501-2015
文摘Objective:To evaluate the antibacterial and cytotoxic effect of Myrciaria dubia(Camu camu)(M.dubia) methanol extract,against Streptococcus mutans(ATCC 25175)(S.mutans) and Streptococcus sanguinis(ATCC 10556)(S.sanguinis).Methods:Two methanol extracts of M.dubia were prepared in vitro,from the seeds and pulp.Ten independent tests were prepared for each type of extract,using 0.12% chlorhexidine solution as positive control.Agar diffusion test was used by preparing wells with the experimental solutions cultivated in anaerobic conditions for 48 h at 37C.Meanwhile,the minimum inhibitory concentration and the cytotoxic effect over MDCK cell line was found.Results:A higher antibacterial effect was observed with the methanol seed extract with an inhibitory halo of(21.36 ± 6.35) mm and(19.21 ± 5.18) mm against S.mutans and S.sanguinis,respectively.The methanol extract of the pulp had an effect of(16.20 ± 2.08)mm and(19.34 ± 2.90) mm,respectively.The minimum inhibitory concentration of the pulp extract was 62.5 mg/mL for both strains,whereas for the seed antibacterial activity was observed even at low concentrations.The CC50 of the seeds extract was at a higher concentration than 800 mg/mL and 524.37 mg/mL for the pulp extract.Conclusions:The experimental findings demonstrated the antibacterial effect of the methanol extract of M.dubia against S.mutans and S.sanguinis.These extracts were not cytotoxic at high concentrations.
文摘为改善3年生嘉宝果(Myrciaria cauliflora Berg)的栽培基质并促进其生长和发育,通过田间盆栽试验研究了有机碳对其实生苗地上部生长量和叶绿素含量的影响。结果表明:有机碳对嘉宝果实生苗地上部生长量和叶绿素含量均有一定的促进作用,以每盆150 m L有机碳效果最为显著。生产中将有机碳剂量控制在每盆100~200 m L对改良嘉宝果生长的土壤基质最为适宜,能有效促进其生长发育。
