Optimization of Extraction Process and Determination of Antioxidant Activity of Myrica ruba Leaf Pigment by Orthogonal Design

[Objectives] To determine the optimum conditions and antioxidant activity of Myrica ruba leaf pigment,and provide reference for further development and use of Myrica ruba leaves. [Methods]Pigment of Myrica ruba leaves was extracted by ethanol extraction method. Absorbance was measured at wavelength of 300 nm. Effects of ethanol volume fraction,solid-to-liquid ratio,temperature,and extraction time were studied. L_9( 3~3) orthogonal experiment was carried out to optimize the extraction process,and DPPH scavenging ability was measured.[Results]The optimum extraction process of Myrica ruba leaf pigment was as follows: the ethanol volume fraction was 50%; the solid-to-liquid ratio was 1: 60( w/v),extraction temperature 70℃; extraction time was 60 min; after the concentration of purified Myrica ruba leaf pigment reached 0. 4455 mg/mL,the DPPH scavenging ability was basically the same as the same concentration Vc.[Conclusions] The experiment indicates that the optimized extraction process has a high yield rate,and Myrica ruba leaf pigment has high anti-oxidant activity.

杨梅叶提取物抗氧化活性的研究

本文从杨梅叶中提取酚类物质,用化学发光法测定了提取物对羟自由基(·OH)和超氧阴离子(O2 )的清除作用,同时将提取物添加到油脂中,研究其抑制物油脂氧化的能力。结果显示,杨梅叶提取物具有很强的抗氧化活性,并且随时期变化有较大差异,但总的来说与酚类物质的含量存正相关性。-·

杨梅叶黄酮类化合物最佳提取工艺研究

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