Background:Prostate cancer(PCa)is one of the most common malignancies of the male genitourinary system.Commiphora myrrha(CM)has the potential to treat PCa,but the underlying mechanism is still unclear.Therefore,this s...Background:Prostate cancer(PCa)is one of the most common malignancies of the male genitourinary system.Commiphora myrrha(CM)has the potential to treat PCa,but the underlying mechanism is still unclear.Therefore,this study uses the network pharmacology method to investigate the target of CM in the treatment of PCa and related signal pathways,and further analyze the theoretical basis and potential mechanism of its treatment with PCa.Methods:All the components and targets of CM were retrieved from Traditional Chinese Medicine System Pharmacology Database and Analysis Platform(TCMSP).GeneCards and DisGeNET databases were used to collect PCa-related targets.String was used to build a target protein interaction network.The network of“active component-interaction target-related pathways”and protein-protein interaction network were constructed using Cytoscape,the functional enrichment analysis of GO and the enrichment analysis of KEGG pathway of CM in the treatment of PCa were performed using Metascape.Results:A total of 33 active ingredients including quercetin,β-sitosterol and ellagic acid were obtained,and 61 common targets of CM for PCa were obtained by screening intersection.The most frequent occurrences were AKT1,TP53,JUN,VEGFA,etc.These targets were mainly involved in biological processes,such as apoptotic signaling pathway,response to growth factor,and reactive oxygen species metabolic process,and were mainly concentrated in PI3K-Akt,MAPK and other signaling pathways.Conclusion:This study revealed the effective ingredients,potential targets and mechanism of action of CM in the treatment of PCa,aiming to provide a theoretical basis and reference for subsequent experimental studies.展开更多
Objective:To investigate the efficacy of frankincense and myrrha in the treatment of acute interstitial cystitis/painful bladder syndrome(IC/PBS).Methods:The effects of frankincense and myrrha on the proliferation and...Objective:To investigate the efficacy of frankincense and myrrha in the treatment of acute interstitial cystitis/painful bladder syndrome(IC/PBS).Methods:The effects of frankincense and myrrha on the proliferation and migration of primary human urothelial cells(HUCs)were assessed in vitro.In the animal study,48 virgin female rats were randomized into 4 groups(12 in each group):(1)control group(saline-injected control);(2)cyclophosphamide(CYP)group(intraperitoneal injected 150 mg/kg CYP);(3)CYP+pentosan polysulfate sodium group(orally received 50 mg/kg pentosan polysulfate sodium);and(4)CYP+frankincense and myrrha group[orally received frankincense(200 mg/kg)and myrrha(200 mg/kg)].Rats orally received pentosan polysulfate sodium or frankincense and myrrha on day 1,2,and 3.The experiments were performed on day 4.Pain and cystometry assessment behavior test were performed.Voiding interval values were assessed in rats under anesthesia.Finally,immunohistochemistry and Western blot were used to confirm the location and level,respectively,of cell junction-associated protein zonula occludens-2(ZO-2)expression.Results:Low dose frankincense and myrrha increased cell proliferation and migration in HUCs compared with control(P<0.05).Rats with acute IC/PBS rats exhibited lower voiding interval values,pain tolerance,and ZO-2 expression(P<0.05).Voiding interval values and pain tolerance were higher in the frankincense and myrrha group than CYP group(P<0.05).ZO-2 expression in the bladder was increased in the CYP+pentosan polysulfate and frankincense+myrrha groups compared with the CYP-induced acute IC/PBS group(P<0.05).Conclusion:frankincense and myrrha modulate urothelial wound healing,which ameliorates typical features of acute IC/PBS in rats.展开更多
文摘Background:Prostate cancer(PCa)is one of the most common malignancies of the male genitourinary system.Commiphora myrrha(CM)has the potential to treat PCa,but the underlying mechanism is still unclear.Therefore,this study uses the network pharmacology method to investigate the target of CM in the treatment of PCa and related signal pathways,and further analyze the theoretical basis and potential mechanism of its treatment with PCa.Methods:All the components and targets of CM were retrieved from Traditional Chinese Medicine System Pharmacology Database and Analysis Platform(TCMSP).GeneCards and DisGeNET databases were used to collect PCa-related targets.String was used to build a target protein interaction network.The network of“active component-interaction target-related pathways”and protein-protein interaction network were constructed using Cytoscape,the functional enrichment analysis of GO and the enrichment analysis of KEGG pathway of CM in the treatment of PCa were performed using Metascape.Results:A total of 33 active ingredients including quercetin,β-sitosterol and ellagic acid were obtained,and 61 common targets of CM for PCa were obtained by screening intersection.The most frequent occurrences were AKT1,TP53,JUN,VEGFA,etc.These targets were mainly involved in biological processes,such as apoptotic signaling pathway,response to growth factor,and reactive oxygen species metabolic process,and were mainly concentrated in PI3K-Akt,MAPK and other signaling pathways.Conclusion:This study revealed the effective ingredients,potential targets and mechanism of action of CM in the treatment of PCa,aiming to provide a theoretical basis and reference for subsequent experimental studies.
基金Supported in part by China Medical University Hospital(No.DMR-109-085)Taiwan Ministry of Science and Technology(No.MOST 107-2314-B-039-036 and MOST 107-2320-B-039-034)。
文摘Objective:To investigate the efficacy of frankincense and myrrha in the treatment of acute interstitial cystitis/painful bladder syndrome(IC/PBS).Methods:The effects of frankincense and myrrha on the proliferation and migration of primary human urothelial cells(HUCs)were assessed in vitro.In the animal study,48 virgin female rats were randomized into 4 groups(12 in each group):(1)control group(saline-injected control);(2)cyclophosphamide(CYP)group(intraperitoneal injected 150 mg/kg CYP);(3)CYP+pentosan polysulfate sodium group(orally received 50 mg/kg pentosan polysulfate sodium);and(4)CYP+frankincense and myrrha group[orally received frankincense(200 mg/kg)and myrrha(200 mg/kg)].Rats orally received pentosan polysulfate sodium or frankincense and myrrha on day 1,2,and 3.The experiments were performed on day 4.Pain and cystometry assessment behavior test were performed.Voiding interval values were assessed in rats under anesthesia.Finally,immunohistochemistry and Western blot were used to confirm the location and level,respectively,of cell junction-associated protein zonula occludens-2(ZO-2)expression.Results:Low dose frankincense and myrrha increased cell proliferation and migration in HUCs compared with control(P<0.05).Rats with acute IC/PBS rats exhibited lower voiding interval values,pain tolerance,and ZO-2 expression(P<0.05).Voiding interval values and pain tolerance were higher in the frankincense and myrrha group than CYP group(P<0.05).ZO-2 expression in the bladder was increased in the CYP+pentosan polysulfate and frankincense+myrrha groups compared with the CYP-induced acute IC/PBS group(P<0.05).Conclusion:frankincense and myrrha modulate urothelial wound healing,which ameliorates typical features of acute IC/PBS in rats.
