The seasonal fluctuation of the plasmodia ofMyxobolus gibelioiWu et Wang, 1982 in the gill filaments of the allogynogenetic gibel carpCarassius auratus gibelio(Bloch) in a fish pond in Hubei Province of China was inve...The seasonal fluctuation of the plasmodia ofMyxobolus gibelioiWu et Wang, 1982 in the gill filaments of the allogynogenetic gibel carpCarassius auratus gibelio(Bloch) in a fish pond in Hubei Province of China was investigated from August 1999 to July 2000. A total of 445 fish was examined; the overall prevalence of the plasmodium infection in the fish was 64.94% and mean abundance of plasmodia was 11.65 ± 27.85 (mean ± SD). Significant seasonal changes in prevalence and mean abundance, with higher levels of the plasmodia infection from late spring to autumn, were observed.展开更多
A poor environment increases fish’s susceptibility to myxosporean infection that can cause the death of larval fish,especially for koi fish(Cyprinus carpio).This study aimed to determine the effect of probiotics,loca...A poor environment increases fish’s susceptibility to myxosporean infection that can cause the death of larval fish,especially for koi fish(Cyprinus carpio).This study aimed to determine the effect of probiotics,local anti-parasitic drugs(kutuklin),and the chemical compound diflubenzuron treatments on the koi immune response.This study used PCR with specific primer 18S SSU rDNA and DNA sequencing to detect Myxobulus phylogenetic.The treatments were divided into 5 groups:Treatment(A)(healthy koi without treatment),(B)(infected koi without treatment),(C)(infected koi with 0.55 mL/30 L probiotics),(D)(infected koi with 1μL/g of feed kutuklin),and(E)(infected koi with 0.02 mg/5 L dimilin).Myxospore has observed with Scanning Electron Microscopy(SEM)and 4′,6-diamidino-2-phenylindole(DAPI)fluorescence staining.The histological analysis using semi-quantitative scoring methods,and flow cytometry was conducted to analyse the immune response of Cluster of differentiation 4(CD4^(+)),Cluster of differentiation 8(CD8^(+)),Tumor Necrosis Factor-alpha(TNF-α),Interferon gamma(IFN-γ)cells in the gills.Results show that the histological analysis indicated edema,hyperplasia,lamella fusion,congestion,and hypertrophy lesions in infected koi.Treatment with probiotics shows the lowest damage(30.6%).The immune responses of CD4^(+)and CD8^(+)cells to dimilin treatment were 10.54%and 16.86%,respectively.The largest TNF-αand IFN-γresponse were for the kutuklin treatment(29.26%)and probiotics treatment(8.23%).展开更多
本实验利用现行主流分类衍征,重新对荆州碘泡虫(Myxobolus kinchowensis)进行了详细描述,其分类特征如下:包囊圆形,寄生于鲫肌肉与肾脏,肌肉包囊大小(126.7±1.8)μm,肾脏包囊直径为94.2μm;两寄生部位各形态参数无显著差异,成熟孢...本实验利用现行主流分类衍征,重新对荆州碘泡虫(Myxobolus kinchowensis)进行了详细描述,其分类特征如下:包囊圆形,寄生于鲫肌肉与肾脏,肌肉包囊大小(126.7±1.8)μm,肾脏包囊直径为94.2μm;两寄生部位各形态参数无显著差异,成熟孢子正面观呈梨形,缝面观呈纺锤形,含一大一小两梨形极囊;无明显囊间突起,孢子后端无褶皱,无粘液膜。组织病理显示在两寄生部位均未引起严重的炎性反应,且感染强度不高,推测该种对宿主无显著影响。基于18S r DNA进化分析发现荆州碘泡虫与寄生在肌肉部位的碘泡虫属种类聚为一个大枝,然后该大枝又根据地理位置远近分为北美枝、欧洲枝以及亚洲枝。通过形态比较研究以及分子分析可确定荆州碘泡虫为有效种,其寄主为鲫,寄生部位为肌纤维间和肾小囊。此外,两组织差异明显的寄生部位出现说明荆州碘泡虫相应的放射孢子虫在宿主鲫体内存在不同的发育与移行途径,而肌肉可能为其常规寄生部位,肾脏为其异常寄生部位,这种寄生部位的转移与宿主转变可能是粘孢子虫物种多样性形成的机制。展开更多
文摘The seasonal fluctuation of the plasmodia ofMyxobolus gibelioiWu et Wang, 1982 in the gill filaments of the allogynogenetic gibel carpCarassius auratus gibelio(Bloch) in a fish pond in Hubei Province of China was investigated from August 1999 to July 2000. A total of 445 fish was examined; the overall prevalence of the plasmodium infection in the fish was 64.94% and mean abundance of plasmodia was 11.65 ± 27.85 (mean ± SD). Significant seasonal changes in prevalence and mean abundance, with higher levels of the plasmodia infection from late spring to autumn, were observed.
基金The authors would like to express their gratitudes to the Institute for Research and Community Service,Universitas Brawijaya,Indonesia through the“Doktor Mengabdi 2018"[grant DIPA number:DIPA-042.01.2.400919,2018].
文摘A poor environment increases fish’s susceptibility to myxosporean infection that can cause the death of larval fish,especially for koi fish(Cyprinus carpio).This study aimed to determine the effect of probiotics,local anti-parasitic drugs(kutuklin),and the chemical compound diflubenzuron treatments on the koi immune response.This study used PCR with specific primer 18S SSU rDNA and DNA sequencing to detect Myxobulus phylogenetic.The treatments were divided into 5 groups:Treatment(A)(healthy koi without treatment),(B)(infected koi without treatment),(C)(infected koi with 0.55 mL/30 L probiotics),(D)(infected koi with 1μL/g of feed kutuklin),and(E)(infected koi with 0.02 mg/5 L dimilin).Myxospore has observed with Scanning Electron Microscopy(SEM)and 4′,6-diamidino-2-phenylindole(DAPI)fluorescence staining.The histological analysis using semi-quantitative scoring methods,and flow cytometry was conducted to analyse the immune response of Cluster of differentiation 4(CD4^(+)),Cluster of differentiation 8(CD8^(+)),Tumor Necrosis Factor-alpha(TNF-α),Interferon gamma(IFN-γ)cells in the gills.Results show that the histological analysis indicated edema,hyperplasia,lamella fusion,congestion,and hypertrophy lesions in infected koi.Treatment with probiotics shows the lowest damage(30.6%).The immune responses of CD4^(+)and CD8^(+)cells to dimilin treatment were 10.54%and 16.86%,respectively.The largest TNF-αand IFN-γresponse were for the kutuklin treatment(29.26%)and probiotics treatment(8.23%).
文摘本实验利用现行主流分类衍征,重新对荆州碘泡虫(Myxobolus kinchowensis)进行了详细描述,其分类特征如下:包囊圆形,寄生于鲫肌肉与肾脏,肌肉包囊大小(126.7±1.8)μm,肾脏包囊直径为94.2μm;两寄生部位各形态参数无显著差异,成熟孢子正面观呈梨形,缝面观呈纺锤形,含一大一小两梨形极囊;无明显囊间突起,孢子后端无褶皱,无粘液膜。组织病理显示在两寄生部位均未引起严重的炎性反应,且感染强度不高,推测该种对宿主无显著影响。基于18S r DNA进化分析发现荆州碘泡虫与寄生在肌肉部位的碘泡虫属种类聚为一个大枝,然后该大枝又根据地理位置远近分为北美枝、欧洲枝以及亚洲枝。通过形态比较研究以及分子分析可确定荆州碘泡虫为有效种,其寄主为鲫,寄生部位为肌纤维间和肾小囊。此外,两组织差异明显的寄生部位出现说明荆州碘泡虫相应的放射孢子虫在宿主鲫体内存在不同的发育与移行途径,而肌肉可能为其常规寄生部位,肾脏为其异常寄生部位,这种寄生部位的转移与宿主转变可能是粘孢子虫物种多样性形成的机制。
