The present paper covers the reversed phase HPLC separation and determination of fourteen derivatized amino acids with a new reagent N hydroxy succinimidyl 3 indolylacetate(SIIA). The derivatives of the amino...The present paper covers the reversed phase HPLC separation and determination of fourteen derivatized amino acids with a new reagent N hydroxy succinimidyl 3 indolylacetate(SIIA). The derivatives of the amino acids were eluted by a mobile phase of methanol water containing 10 mmol/L H 3cit Na 2HPO 4 buffer of pH 3 5 and 1 2 mmol/L tetrabutylammonium bromide on an ODS column. The detection limits were pmol level or so, respectively, when a signal to noise ratio was 3. The method was applied to the determination of amino acids in tea sample with satisfactory results.展开更多
文摘The present paper covers the reversed phase HPLC separation and determination of fourteen derivatized amino acids with a new reagent N hydroxy succinimidyl 3 indolylacetate(SIIA). The derivatives of the amino acids were eluted by a mobile phase of methanol water containing 10 mmol/L H 3cit Na 2HPO 4 buffer of pH 3 5 and 1 2 mmol/L tetrabutylammonium bromide on an ODS column. The detection limits were pmol level or so, respectively, when a signal to noise ratio was 3. The method was applied to the determination of amino acids in tea sample with satisfactory results.
