Optimizing N fertilizer use for sesame under rain fed and irrigation conditions in Northern Ethiopia

Inappropriate use of fertilizers is one of the major production constraints in sesame. Studies on N fertilizer optimization on sesame were conducted at Humera Agricultural Research Center(Hu ARC) under rain fed and irrigation conditions. Thirteen(13) N doses were evaluated in a Randomized Complete Block Design(RCBD)during 2016–2018 for rainfed conditions and 2017 to 2019 for irrigation conditions. The study was conducted with objective to optimize N fertilizer use for sesame. In the rainfed condition, the results demonstrated a prolonged duration to reach 50% flowering with higher nitrogen(N) application rates. The application of 52.5–110kg N ha^(-1) resulted in significantly higher seed yield, while lower(18 kg N ha^(-1)) and higher(156 kg N ha^(-1)) doses of N led to reduced seed yield. Under irrigation conditions, superior seed weights and maximum seed yield were observed at 64 and 75 kg N ha^(-1), whereas lower N doses resulted in diminished seed yield. The agronomic efficiency of N fertilizer(N-AE) was found to be highest at the rate of 64 kg N ha^(-1) under both growing conditions.The partial budget analysis revealed that applying 64 kg N ha^(-1) for rainfed cultivation and between 64 and 75 kg N ha^(-1) for irrigated sesame production yielded greater net profit, MRR, and residual ranking. Therefore, it is recommended to apply a rate of 64 kg N ha^(-1) for rainfed sesame cultivation and between 64 up to 75 kg N ha^(-1) for the irrigated sesame inorder to increase the productivity of this crop.

Effects of Nitrogen Fertilizer Reduction and Application of Nitrogen Fertilizer as Base Fertilizer on Rapeseed Yield and Nitrogen Absorption

[Objective] The aim was to research effects of N fertilizer reduction and application of N fertilizer （as base fertilizer） on rapeseed yield and N absorption. [Method] Based on Ganyouza No.5, the ratio of N, P2O5 andK2O was set at 1：0.5：0.5; N fertilizers were set involving reduced quantity at 150 kg/hm2 and preferred quantity at 180 kg/hm2; 100%, 80% and 60% of N fertilizers were applied as base fertilizers in the test respectively. In general, field tests were conducted to explore effects of reduced N fertilizer and application of N fertilizer as base fertilizer on rapeseed yield and N absorption. [Resalt] When applied N fertilizer as base fertilizer was the same, plant height, stem diameter, length of major inflorescence, number of effective branch, pod number per plant, seed number per pod, and biomass yield in group with preferred N quantity were significantly higher than those in group with reduced N fertilizer. Rapeseed yield and profits in group with preferred N quantity were signifi- cantly higher than those in group with reduced N fertilizer in field with moderate fertili- ty. In fields with higher fertility, however, the two factors were just a little higher. In group with reduced N fertilizer, use efficiency of N fertilizer, N uptake efficiency, par- tial factor productivity and harvest index of N were all significantly higher than those in group with preferred N fertilizer. Agronomic nitrogen use efficiency in group with preferred N fertilizer was significantly higher than that in group with reduced N fertiliz- er in field with moderate fertility and was significantly lower in field with high yield. With amounts of N, P and K fertilizers fixed, economic characters, yield constitution, yield, profits and N absorption in group, where 60% of N fertilizers were applied as base fertilizer, were significantly higher than those in groups with 80% or 100% of base fertilizer （N fertilizers）. These indicated that rational fertilization would maintain rapeseed yield high and reduce N input to improve use efficiency of N fertilizer. On the other hand, it is effective to improve rapeseed yield.＇to reduce N fertilizer to 150 kg/hm2, and application of 60%. of N fertilizers as base fertilizer is still proved optimal at present. [Conclusion] The research provides.theoretioal and technical references for improvement of yield of rapeseed and efficiency of N fertilizer.

Effect of N Fertilizers on Root Growth and Endogenous Hormones in Strawberry

Endogenous hormones play an important role in the growth and development of roots. The objective of this research was to study the effect of four types of N fertilizers on the root growth of strawberry (Fragaria ananassa Duchesne) and the endogenous enzymes of indole-3-acetic acid (IAA), abscisic acid (ABA), and isopentenyl adenosine (iPA) in its roots and leaves using enzyme-linked immunosorbent assay. Application of all types of N fertilizers significantly depressed (P ≤ 0.05) root growth at 20 d after transplanting. Application of organic-inorganic fertilizer (OIF) as basal fertilizer had a significant negative effect (P ≤ 0.05) on root growth. The application of OIF and urea lowered the lateral root frequency in strawberry plants at 60 d (P ≤ 0.05) compared with the application of two organic fertilizers (OFA and OFB) and the control (CK). With the fertilizer treatments, there were the same concentrations of IAA and ABA in both roots and leaves at the initial growth stage (20 d), lower levels of IAA and ABA at the later stage (60 d), and higher iPA levels at all seedling stages as compared to those of CK. Thus, changes in the concentrations of endogenous phytohormones in strawberry plants could be responsible for the morphological changes of roots due to fertilization.

Effects of long-term amendment of organic manure and nitrogen fertilizer on nitrous oxide emission in a sandy loam soil

To understand the effects of long-term amendment of organic manure and N fertilizer on N2O emission in the North China Plain, a laboratory incubation at different temperatures and soil moistures were carried out using soils treated with organic manure （OM）, half organic manure plus half fertilizer N （HOM）, fertilizer NPK （NPK）, fertilizer NP （NP）, fertilizer NK （NK）, fertilizer PK （NK） and control （CK） since 1989. Cumulative N2O emission in OM soil during the 17 d incubation period was slightly higher than in NPK soil under optimum nitrification conditions （25℃ and 60% water-filled pore space, WFPS）, but more than twice under the optimum denitrification conditions （35℃ and 90% WFPS）. N2O produced by denitrification was 2.1-2.3 times greater than that by nitrification in OM and HOM soils, but only 1.5 times greater in NPK and NP soils. These results implied that the long-term amendment of organic manure could significantly increase the N2O emission via denitrification in OM soil as compared to NPK soil. This is quite different from field measurement between OM soil and NPK soil. Substantial inhibition of the formation of anaerobic environment for denitrification in field might result in no marked difference in N2O emission between OM and NPK soils. This is due in part to more rapid oxygen diffusion in coarse textured soils than consumption by aerobic microbes until WFPS was 75% and to low easily decomposed organic C of organic manure. This finding suggested that addition of organic manure in the tested sandy loam might be a good management option since it seldom caused a burst of N2O emission but sequestered atmospheric C and maintained efficiently applied N in soil.

Comparison of Mathematical Models for Describing CropResponses to N Fertilizer

Four mathematical models were systematically evaluated in describing responses of four different cropsat 7 rates of nitrogen application. Residual sum of squares and a total point ranking method were used toassess the model fitting for crop responses to nitrogen application. Sparrow’s inverse quadratic polynomialmodel performed the best.

Evaluation of a model recommended for N fertilizer application in irrigated rice

The response of rice to N fertilizer applicationhas shown that high rates of N application donot always ensure a proportional increase inyield due to high N losses. A model, ORYZA-0 was developed by ten Berge for designingoptimum N fertilizer management strategy inrice. We evaluated the performance ofORYZA-0 in Jinhua, Zhejiang Province. ORYZA-0 includes N uptakes, partition-ing of N among the organs, and utilization ofleaf N in converting solar energy to dry mat-ter. It can predict the amount and time of Nfertilizer application to achieve a maximumbiomass or yield combining with Price algo-rithm optimization procedure.

An assessment of response of soil-based indicators to nitrogen fertilizer across four tropical eucalyptus plantations

Low nitrogen （N） availability often results in reduced productivity of Eucalyptus plantations. We studied the response of four eucalyptus plantations （two plantations of E. tereticornis on the coastal lowlands, and two plantations of E. grandis in the upland region of the Western Ghats, Kerala, India） to N addition and related this response to seasonal N mineralization as well as other indices of N availability, in order to examine the utility of soil based indicators of N mineralization for predicting the response of eucalyptus growth to added N ferti- lizer. Several biochemical indicators were examined for their capacity to predict response to N fertilizer, including total soil N, soil C：N ratio, and N released during anaerobic and aerobic incubation. Results show that nitrogen fertilizer addition increased productivity across the 4 sites from 7% to 70%, N released during an aerobic incubation had the highest correlation with fertilizer response across the 4 sites （R^2=0.92/ p〈0.01）, and that Modelled seasonal soil N mineralisation was a poorer predictor of fertilizer response than N released during an aerobic incubation. Whilst some of these indicators are promising, they need wider validation and testing before they could be routinely applied.

An assessment of response of soil-based indicators to nitrogen fertilizer across four tropical eucalyptus plantations

Low nitrogen (N) availability often results in reduced productivity of Eucalyptus plantations. We studied the response of four eucalyptus plantations (two plantations of E. tereticornis on the coastal lowlands, and two plantations of E. grandis in the upland region of the Western Ghats, Kerala, India) to N addition and related this response to seasonal N mineralization as well as other indices of N availability, in order to examine the utility of soil based indicators of N mineralization for predicting the response of eucalyptus growth to added N fertilizer. Several biochemical indicators were examined for their capacity to predict response to N fertilizer, including total soil N, soil C:N ratio, and N released during anaerobic and aerobic incubation. Results show that nitrogen fertilizer addition increased productivity across the 4 sites from 7% to 70%, N released during an aerobic incubation had the highest correlation with fertilizer response across the 4 sites (R2=0.92, p<0.01), and that Modelled seasonal soil N mineralisation was a poorer predictor of fertilizer response than N released during an aerobic incubation. Whilst some of these indicators are promising, they need wider validation and testing before they could be routinely applied.

Fate of fertilizer nitrogen and residual nitrogen in paddy soil in Northeast China

The relationship between the fate of nitrogen (N) fertilizer and the N application rate in paddy fields in Northeast China is unclear,as is the fate of residual N.To clarify these issues,paddy field and15N microplot experiments were carried out in 2017 and 2018,with N applications at five levels:0,75,105,135 and 165 kg N ha–1(N0,N75,N105,N135 and N165,respectively).15N-labeled urea was applied to the microplots in 2017,and the same amount of unlabeled urea was applied in 2018.Ammonia (NH3) volatilization,leaching,surface runoff,rice yield,the N contents and15N abundances of both plants and soil were analyzed.The results indicated a linear platform model for rice yield and the application rate of N fertilizer,and the optimal rate was 135 kg N ha–1.N uptake increased with an increasing N rate,and the recovery efficiency of applied N (REN) values of the difference subtraction method were 45.23 and 56.98%on average in 2017and 2018,respectively.The RENwas the highest at the N rate of 135 kg ha–1in 2017 and it was insignificantly affected by the N application rate in 2018,while the agronomic efficiency of applied N (AEN) and physiological efficiency of applied N (PEN) decreased significantly when excessive N was applied.N loss through NH3volatilization,leaching and surface runoff was low in the paddy fields in Northeast China.NH3volatilization accounted for 0.81 and 2.99%of the total N application in 2017 and 2018,respectively.On average,the leaching and surface runoff rates were 4.45% and less than 1.05%,respectively,but the apparent denitrification loss was approximately 42.63%.The residual N fertilizer in the soil layer (0–40 cm) was 18.37–31.81 kg N ha–1in 2017,and the residual rate was 19.28–24.50%.Residual15N from fertilizer in the soil increased significantly with increasing N fertilizer,which was mainly concentrated in the 0–10 cm soil layer,accounting for 58.45–83.54% of the total residual N,and decreased with increasing depth.While the ratio of residual N in the 0–10 cm soil layer to that in the 0–40 cm soil layer was decreased with increasing N application.Furthermore,of the residual N,approximately 5.4%was taken up on average in the following season and 50.2%was lost,but 44.4%remained in the soil.Hence,the amount of applied N fertilizer should be reduced appropriately due to the high residual N in paddy fields in Northeast China.The appropriate N fertilizer rate in the northern fields in China was determined to be 105–135 kg N ha–1in order to achieve a balance between rice yield and high N fertilizer uptake.

Effects of N,P and K Fertilizers on Growth of Clover Nitrogen-fixing Rhizobia and Soil Fertility after Plantation

In order to develop organic rice and increase paddy soil fertility by cloverorganic rice rotation, the effects of N, P and K fertilizers on growth of clover nitrogen-fixing rhizobia and soil fertility after plantation were investigated, thereby providing certain reference for reasonable cultivation of clover and improvement of soil fertility. A two-year experiment was conducted from 2012 to 2013. The clover was cultivated after rice every year, and different levels of N, P and K fertilizers were applied before winter. In the treatment group, no fertilizer was applied. The effects of different fertilizers and different application amounts on clover yield, nitrogen-fixing rhizobia quantity, nitrogen-fixing rhizobia weight and soil fertility after plantation were analyzed. The results showed that the application of N, P and K fertilizers in the early stage had significantly effect on the growth of clover. When the application amount of N fertilizer was 75 kg/hm^2（N 46%）, the clover yield, nitrogen-fixing rhizobia quantity and nitrogen-fixing rhizobia weight were highest. The soil nitrogen content after plantation increased with the increase of the application amount of N fertilizer, while the P and K content decreased and then increased with the increased application amounts. Soil available P content increased with the increased application amounts of N and P fertilizers, but it did not change significantly with the increased application amount of K fertilizer. Soil available K content increased first and then decreased with the increased application amounts of N and P fertilizers.When the application amounts of N and P fertilizers were 150（N 46%） and 300（P_2O_5 12%） kg/hm^2, soil available K content reached the maximum. Soil organic matter content increased with the increased application amounts of N, P and K fertilizers. Therefore, in the cultivation of clover, appropriate application of N, P and K fertilizers in the early stage can improve clover yield and soil fertility.

Long-Term Application of Organic Manure and Mineral Fertilizer on N_2O and CO_2 Emissions in a Red Soil from Cultivated Maize-Wheat Rotation in China

A long-term field experiment was established to determine the influence of mineral fertilizer and organic manure on soil fertility. A tract of red soil （Ferralic Cambisol） in Qiyang Red Soil Experimental Station （Qiyang County, Hunan Province, China） was fertilized beginning in 1990 and N20 and CO2 were examined during the maize and wheat growth season of 2007-2008. The study involved five treatments： organic manure （NPKM）, fertilizer NPK （NPK）, fertilizer NP （NP）, fertilizer NK （NK）, and control （CK）. Manured soils had higher crop biomass, organic C, and pH than soils receiving the various mineralized fertilizers indicating that long-term application of manures could efficiently prevent red soil acidification and increase crop productivity. The application of manures and fertilizers at a rate of 300 kg N ha-1 yr-1 obviously increased NzO and CO2 emissions from 0.58 kg N20-N ha-~ yr-~ and 10565 kg C ha-~ yr-~ in the CK treatment soil to 3.0l kg N20-N ha-~ yr-~ and 28 663 kg C ha-~ yr-I in the NPKM treatment. There were also obvious different effects on N20 and CO2 emissions between applying fertilizer and manure. More N20 and CO2 released during the 184-d maize growing season than the 125- d wheat growth season in the manure fertilized soils but not in mineral fertilizer treatments. N20 emission was significantly affected by soil moisture only during the wheat growing season, and CO2 emission was affected by soil temperature only in CK and NP treatment during the wheat and maize growing season. In sum, this study indicates the application of organic manure may be a preferred strategy for maintaining red emissions than treatments only with mineral fertilizer. soil productivity, but may result in greater N20 and CO2

Effect of N and K Fertilizers on Yield and Quality of Greenhouse Vegetable Crops

The application of large amounts of fertilizers, a conventional practice in northern China for the production of vegetable crops, generally leads to substantial accumulation of soil nutrients within a relatively short period of time. A fixed field experiment was designed to study the effects of nitrogen （N） and potassium （K） fertilizers applied to optimize the yield and quality of typical vegetable crops. Application of N and K fertilizers significantly increased the yields of kidney bean. The largest yields were obtained in the first and second years after application of 1 500 kg N and 300 kg K20 ha^-1. In the third year, however, there was a general decline in yields. Maximum yields occurred when intermediate rates of N and K （750 kg N and 300 kg K20 ha 1） were applied. However, no significant differences were observed in the concentrations of vitamin C （VC） in kidney bean among different years and various rates of fertilizer treatments. Yields of tomato grown in rotation after kidney bean showed significant responses to the application of N and K in the first year. In the second year, the yields of tomato were much lower. This suggested that the application of N fertilizer did not have any effect upon tomato yield, whereas application of K fertilizer did increase the yield. Application of K fertilizer was often associated with increased sugar concentrations.

Evaluation of N Fertilizers Effects on Grape Based on the Expression of N Metabolic Genes

In order to study the influence of different types of N fertilizers on grapevine(Vitis labruscana ‘Black Summer') trees were used as experiment materials and fertilizers foliar application trails were conducted in the flowering and veraison stages. The expression levels of five grapevine N metabolic genes, including Vv GHD, Vv Ni R, Vv NR, Vv GS and Vv AS, were analyzed, some physiological traits including the flower and fruit dropping rate, leaves and shoots growth rate, chlorophyll content and the fruit size of grape berry were also investigated. The results showed that,in general, the expression levels of the five N metabolic genes were increased after foliar applied different types of N fertilizers in the two periods.N metabolic genes showed similar expression patterns toward the same type of fertilizer in the two periods, whereas foliar applied different types of N fertilizers, their expression patterns changed. Moreover, the N fertilizer which had stronger and longer influence on the N metabolic genes could enhance the physiology traits more dramatically. Given the gene expression levels and the changes of physiology traits, we conclude that urea and ammonium nitrate have the best effect on grapevine; calcium nitrate can help to reduce the flower and berry dropping rate; ammonium sulphate and sodium nitrate have relatively poor effects on grapevine.

Fertilizer ^(15)N Accumulation, Recovery and Distribution in Cotton Plant as Affected by N Rate and Split

N fertilization of 300 kg N ha-1 is normally applied to cotton crops in three splits： pre-plant application （PPA, 30%）, first bloom application （FBA, 40%） and peak bloom application （PBA, 30%） in the Yangtze River Valley China. However, low fertilizer N plant recovery （NPR） （30-35%） causes problems such as cotton yield stagnation even in higher N rate, low profit margin of cotton production and fertilizer release to the environment. Therefore, it is questioned： Are these three splits the same significance to cotton N uptake and distribution？ An outdoor pot trial was conducted with five N rates and 15 N labeled urea to determine the recovery and distribution of 15N from different splits in cotton （Gossypium hirsutum L. cv. Huazamian H318） plant. The results showed that, cotton plant absorbed fertilizer 15N during the whole growing period, the majority during flowering for 18-20 d regardless of N rates （150-600 kg ha-1）. Fertilizer 15N proportion to the total N accumulated in cotton plant increased with N rates, and it was the highest in reproductive organs （88% averaged across N rates） among all the plant parts. FBA had the highest NPR （70%）, the lowest fertilizer N lose （FNL, 19%）, and the highest contribution to the fertilizer 15N proportion to the total N （46%） in cotton plant, whereas PPA had the reverse effect. It suggests that FBA should be the most important split for N absorption and yield formation comparatively and allocating more fertilizer N for late application from PPA should improve the benefit from fertilizer.

Effects of Postponing N Application on Metabolism,Absorption and Utilization of Nitrogen of Summer Maize in SuperHigh Yield Region

[Objective] The aim was to explore effects of application postponing of N fertilizer and the mechanism of yield increase in order to provide references for N fertilizer application in a rational way. [Method] In a super-high yielded region of summer maize, field experiment was conducted to research effects of N fertilizer postponing on key enzymes of N metabolism, yield of maize and N fertilizer use. [Result] After application of N fertilizer was postponed, NR, SPS and GS activities of ear-leaf of summer maize increased by 11.99%-34.87%, 8.25%-10.64% and 10.00%- 16.81% on the 28^th d of silking; content of soluble sugar in leaves enhanced signifi- cantly and accumulated nitrogen increased by 5.00%-9.74% in mature stage. The postponing fertilization of ＂30% of fertilizer in seedling stage＋30% of fertilizer in flare- opening stage＋40% of fertilizer in silking stage meets N demands of summer maize in late growth period. Compared with conventional fertilization, the maize yield, agro- nomic efficiency and use of N fertilizer all improved by 5.05%, 1.75 kg/kg and 6.87%, respectively, after application postponed. [Conclusion] Application postponing of N fertilizer maintains activity of NR, GS and SPS higher and coordinates metabolism of C and N in late growth period, to further improve yield of maize.

High variation of fungal communities and associated potential plant pathogens induced by long-term addition of N fertilizers rather than P, K fertilizers: A case study in a Mollisol field

Nitrogen(N),phosphate(P),and potassium(K)are the three most important nutrients applied into agricultural soils,but the impacts of their single or combined application on soil fungal community structure and stability are still open questions.Using qPCR and Illumina Miseq sequencing,the variation of soil fungal communities in response to long-term addition of N,P,or K fertilization alone and their combinations in a Mollisol field was investigated in this study.In addition,the fungal community resistance indices and network structure were studied.Results showed that N fertilizations(N,NK,NP and NPK treatments)rather than P,K fertilizations(P,K and PK treatments)significantly increased fungal abundance,but decreased fungal diversity and shifted fungal community structures when compared to non-fertilization(NoF).Additionally,N fertilization treatments presented lower resistance of fungal communities to environment disturbances than those of P,K fertilization treatments.More numbers and higher abundances of changed fungal taxa at the genus and OTU levels were induced by N fertilizations rather than by addition of P,K fertilizers.In addition,N fertilizations induced a more changeable fungal network and complex pathogenic subnetwork with many positive interactions among responding plant pathogens(RP,the changeable plant pathogens induced by fertilizers addition compared to NoF)when compared to P,K fertilizations.These RP directly and negatively influenced fungal community resistance examined by structural equation modeling(SEM),which were indirectly detrimental to soybean yields.Our findings revealed that addition of N fertilizers significantly disturbed fungal communities and promoted pathogenic interactions,and provided insights into the optimization of fertilization strategies toward agricultural sustainability.

Effect of Cl￣ on Behavior of Fertilizer Nitrogen, Nnmberof Microorganisms and Enzyme Activities in Soils

Pot experiments were conducted to study the effect of Cl￣- on transformation of fertilizer N, number ofmicroorganisms and enzyme activities in soils. It is indicated that Cl￣- did not show significant influenceon total number of bacteria, actinomyces and fungi, but significantly reduced the number of nitrosobacteria,which led to decrease of NO content in the soil. Application of Cl￣- to soil could significantly enhance theactivities of phosphatase and urease in the coastal saline soil and orthic aquisols. In hilly red soil, however,the application of Cl￣- at the rate of 500-1000 mg Cl￣- kg￣(-1) soil significantly decreased the activity of thetwo enzymes mentioned above.

Effect of Fertilizer N Forms on Physiological Metabolism and Potassium Uptake of Flue-Cured Tobacco

The growth, chlorophyll composition, photosynthesis, respiration, K uptake and root K+ secretion offlue-cured tobacco as thected by different concentrations of N supplied as urea, NaNO3 and NH4NO3 werestudied under the experimental condition of sand culture. The results showed that the content of K in thefiue-cured tobacco was not merely related with root vitality and uptake but also closely related with root cellmembrane structure and K+ secretion.

Fate of Fertilizer Nitrogen Applied to Crops Grown on Fluvo-Aquic Soil in Huang-Huai-Hai Plain

The fate of urea-and ammonium bicarbonate (ABC)-nitrogen (N) applied by prevailing traditional techniques to winter wheat (Triticum aestivum L.) or maize (Zea mays L.) grown in the fields of Fluvo-aquic soil was investigated using 15N tracer-micro-plot technique. Results show that:(1) at maturity of wheat, N recovery in plants and N losses of urea and ABC applied at seeding in autumn were 31-39%, and 34-46%, respectively, while the corresponding figures for side-banding at 10 cm depth in early spring were 51-57%, and 5-12%; surface-broadcast of urea followed by irrigation at early spring was as efficient as the side-banding in improving N recovery in plants and reducing N loss, however, such technique was found less satisfactory with ABC. (2) At the maturity of maize, N recovery in the plants and N loss of urea and ABC sidebanded at seedling stage or prior to tasseling ranged from 23% to 57%, and 9% to 26%, respectively. (3) Either in Wheat or in maize experiment, the majority of residual fertilizer N in soil profile (0-60 cm) was in the form of biologically immobilized organic N, however, the contribution of ammonium fixation by clay minerals increased markedly with depth in soil profile. (4) Though the proportion of residual fertilizer N was generally highest in the top 20 cm soil layer, considerable reaidual N (mostly 6-11 % of the N applied) was found in 60-100 cm soil layers.

Characterization and QTL identification in eggplant introgression lines under two N fertilization levels

Lowering nitrogen inputs is a major goal for sustainable agriculture.In the present study,a set of 10 Solanum melongena introgression lines(ILs)developed using Solanum incanum as the exotic donor parent were grown under two nitrogen fertilization doses supplied with the irrigation system:1)8.25 mmol·L-1NH4NO3,corresponding to the high nitrogen treatment(HN),and 2)no external nitrogen supply,corresponding to the low nitrogen treatment(LN).Twenty traits,including plant growth and yield parameters,fruit size and morphology,nitrogen and carbon content in leaf and fruit,and phenolics content in fruit,were evaluated.The aim was to select of potential materials for eggplant breeding under low N inputs,as well as to identify and locate putative QTLs associated with the traits evaluated.No significant differences were observed between the soil characteristics of the HN and LN treatments,except for nitrogen and iron content,which was slightly lower in the HN,probably as a consequence of higher nutrient removal from soil by plants in the latter group.Analysis of variance showed that lowering nitrogen inputs did not significantly affect the final yield,fruit morphology,size and phenolics content.Most agronomic traits were highly and positively correlated with each other under both treatments,as well as total phenolics with chlorogenic acid content.The assessment of the differences between each IL and the recipient parent resulted in the identification of 36 QTLs associated with most of the traits—12 were specific to the HN,17 specific to the LN,and 7 were stable across treatments.The introgressed fragment of S.incanum generally had a negative effect on the trait,except for QTLs for fruit dry matter,for fruit length on chromosome 10 under the HN,and for fruit pedicel length on chromosome 9 under the LN.The increase over AN-S-26 of the allele of S.incanum for the QTLs detected ranged between-73.98% and 26.03% in HN and-73.67%and 34.43% in LN.These findings provide useful tools for the utilization of S.incanum in eggplant breeding under lower nitrogen fertilization.