[Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N＋ beam radiation in rice.[Method]Three groups of ion beam irradiation rice seeds and untreated rice seeds w...[Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N＋ beam radiation in rice.[Method]Three groups of ion beam irradiation rice seeds and untreated rice seeds were selected respectively,and then the total RNA of seedlings after 96 h of germination was extracted at 30 ℃.Agilent gene chip was used to screen the differentially expressed genes of two groups of rice seedlings.The chip contained 510 miRNA probes;and about two times of expression differences between two samples were considered as the threshold range.[Result]14 miRNA molecules showed significant expression differences between the irradiated group and the control group,and all of them were decreased.[Conclusion]The miRNAs showed expression differences between irradiated group and the control group,which might regulate the expression of certain genes to respond to ion irradiation,thus producing physiological,biochemical and phenotypic differences.展开更多
Ever since the low energy N + ion beam has been accepted, the mutations of ionizing radiation are attributable mainly to avoidance of DNA damages repair. Evidences based on in vivo proof results are limited. Using the...Ever since the low energy N + ion beam has been accepted, the mutations of ionizing radiation are attributable mainly to avoidance of DNA damages repair. Evidences based on in vivo proof results are limited. Using the E.coli wild type and mutator strains, the mutant frequencies suggest that base substitutions in rpoB gene are induced by the N + implantation. A highly conserved region is selected to get the direct evidence for base substitutions by sequence of the high fidelity PCR amplification products in mutants. Most of the mutants (90.9%, 40/44) have at least one base substitution in the amplification region. The evidences for CG to TA (55%, 22/40), AT to GC (20%, 8/40) and TA to CG (5%, 2/40) transitions are identified. The transversions are AT to TA (15%, 6/40) and GC to CG (5%, 2/40). It is suggested that DNA cytosine methylase might play an important role in mismatch repair of DNA damage induced by N + implantation by analysis of the mutant frequencies of mutator strains.展开更多
In this paper, we discuss the counting prob lem of an order n-group of set (A 1,A 2,…,A n) which satisfies ∪ni=1A i=｛a 1,a 2,…,a m｝ and one of the following: (1) ∩ni=1A i=Φ; (2) ∩ni=1A i=｛b 1,b 2,…,b k｝;(3)...In this paper, we discuss the counting prob lem of an order n-group of set (A 1,A 2,…,A n) which satisfies ∪ni=1A i=｛a 1,a 2,…,a m｝ and one of the following: (1) ∩ni=1A i=Φ; (2) ∩ni=1A i=｛b 1,b 2,…,b k｝;(3) ∩ni=1A 1｛b 1,b 2,…,b k｝; (4) A i≠Φ (i=1,2,…,k). We solve these problems by element analytical meth od.展开更多
In the present study, we prepared novel NGR-modified PEG-PLGA polymeric micelles containing paclitaxel （NGR- PM-PTX） in order to evaluate their potential targeting to aminopeptidase N receptors expressed on tumor en...In the present study, we prepared novel NGR-modified PEG-PLGA polymeric micelles containing paclitaxel （NGR- PM-PTX） in order to evaluate their potential targeting to aminopeptidase N receptors expressed on tumor endothelial cells and the tumor cell surface and its anti-tumor activity in vitro and in vivo. NGR-PM-PTX was prepared by thin-film hydration method. The in vitro targeting characteristics of NGR-modified PM on HUVEC （human umbilical vein endothelial cells）, HT1080 （human fibrosarcoma cells） and MCF-7 （human breast adenocarcinoma cells） were then investigated. The anti-tumor activity of NGR-PM-PTX was evaluated in HT1080 tumor-bearing mice in vivo. The targeting activity of the NGR-modified PM was demonstrated by flow cytometry and confocal microscopy in vitro. NGR-PM-PTX also produced marked anti-tumor activity to HTI080 tumor-beating mice in vivo.展开更多
基金Supported by National Natural Science Foundation of China(308200204)Natural Science Research Program in Education Department of Henan Province(2009B180019)~~
文摘[Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N＋ beam radiation in rice.[Method]Three groups of ion beam irradiation rice seeds and untreated rice seeds were selected respectively,and then the total RNA of seedlings after 96 h of germination was extracted at 30 ℃.Agilent gene chip was used to screen the differentially expressed genes of two groups of rice seedlings.The chip contained 510 miRNA probes;and about two times of expression differences between two samples were considered as the threshold range.[Result]14 miRNA molecules showed significant expression differences between the irradiated group and the control group,and all of them were decreased.[Conclusion]The miRNAs showed expression differences between irradiated group and the control group,which might regulate the expression of certain genes to respond to ion irradiation,thus producing physiological,biochemical and phenotypic differences.
文摘Ever since the low energy N + ion beam has been accepted, the mutations of ionizing radiation are attributable mainly to avoidance of DNA damages repair. Evidences based on in vivo proof results are limited. Using the E.coli wild type and mutator strains, the mutant frequencies suggest that base substitutions in rpoB gene are induced by the N + implantation. A highly conserved region is selected to get the direct evidence for base substitutions by sequence of the high fidelity PCR amplification products in mutants. Most of the mutants (90.9%, 40/44) have at least one base substitution in the amplification region. The evidences for CG to TA (55%, 22/40), AT to GC (20%, 8/40) and TA to CG (5%, 2/40) transitions are identified. The transversions are AT to TA (15%, 6/40) and GC to CG (5%, 2/40). It is suggested that DNA cytosine methylase might play an important role in mismatch repair of DNA damage induced by N + implantation by analysis of the mutant frequencies of mutator strains.
文摘In this paper, we discuss the counting prob lem of an order n-group of set (A 1,A 2,…,A n) which satisfies ∪ni=1A i=｛a 1,a 2,…,a m｝ and one of the following: (1) ∩ni=1A i=Φ; (2) ∩ni=1A i=｛b 1,b 2,…,b k｝;(3) ∩ni=1A 1｛b 1,b 2,…,b k｝; (4) A i≠Φ (i=1,2,…,k). We solve these problems by element analytical meth od.
基金National Natural Science Foundation of China (Grant No.30873170)
文摘In the present study, we prepared novel NGR-modified PEG-PLGA polymeric micelles containing paclitaxel （NGR- PM-PTX） in order to evaluate their potential targeting to aminopeptidase N receptors expressed on tumor endothelial cells and the tumor cell surface and its anti-tumor activity in vitro and in vivo. NGR-PM-PTX was prepared by thin-film hydration method. The in vitro targeting characteristics of NGR-modified PM on HUVEC （human umbilical vein endothelial cells）, HT1080 （human fibrosarcoma cells） and MCF-7 （human breast adenocarcinoma cells） were then investigated. The anti-tumor activity of NGR-PM-PTX was evaluated in HT1080 tumor-bearing mice in vivo. The targeting activity of the NGR-modified PM was demonstrated by flow cytometry and confocal microscopy in vitro. NGR-PM-PTX also produced marked anti-tumor activity to HTI080 tumor-beating mice in vivo.
