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Co-doped BaFe_(2)As_(2) Josephson junction fabricated with a focused helium ion beam 被引量:1
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作者 陈紫雯 张焱 +6 位作者 马平 徐中堂 李宇龙 王越 路建明 马衍伟 甘子钊 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第4期181-186,共6页
Josephson junction plays a key role not only in studying the basic physics of unconventional iron-based superconductors but also in realizing practical application of thin-film based devices,therefore the preparation ... Josephson junction plays a key role not only in studying the basic physics of unconventional iron-based superconductors but also in realizing practical application of thin-film based devices,therefore the preparation of high-quality iron pnictide Josephson junctions is of great importance.In this work,we have successfully fabricated Josephson junctions from Co-doped BaFe_(2)As_(2)thin films using a direct junction fabrication technique which utilizes high energy focused helium ion beam(FHIB).The electrical transport properties were investigated for junctions fabricated with various He^(+)irradiation doses.The junctions show sharp superconducting transition around 24 K with a narrow transition width of 2.5 K,and a dose correlated foot-structure resistance which corresponds to the effective tuning of junction properties by He^(+)irradiation.Significant J_c suppression by more than two orders of magnitude can be achieved by increasing the He^(+)irradiation dose,which is advantageous for the realization of low noise ion pnictide thin film devices.Clear Shapiro steps are observed under 10 GHz microwave irradiation.The above results demonstrate the successful fabrication of high quality and controllable Co-doped BaFe_(2)As_(2)Josephson junction with high reproducibility using the FHIB technique,laying the foundation for future investigating the mechanism of iron-based superconductors,and also the further implementation in various superconducting electronic devices. 展开更多
关键词 focused helium ion beam Co doped BaFe_(2)As_(2) Josephson junction
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Uniformity Control of Scanned Beam in 300 MeV Proton and Heavy Ion Accelerator Complex at SESRI
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作者 HOU Lingxiao YUAN Youjin +10 位作者 SHEN Guodong RUAN Shuang LIU Jie ZHU Yunpeng WANG Geng GUO Hongliang LYU Mingbang GAO Daqing XU Zhiguo SHENG Lina YANG Jiancheng 《原子能科学技术》 EI CAS CSCD 北大核心 2024年第4期705-713,共9页
In recent years,heavy ion accelerator technology has been rapidly developing worldwide and widely applied in the fields of space radiation simulation and particle therapy.Usually,a very high uniformity in the irradiat... In recent years,heavy ion accelerator technology has been rapidly developing worldwide and widely applied in the fields of space radiation simulation and particle therapy.Usually,a very high uniformity in the irradiation area is required for the extracted ion beams,which is crucial because it directly affects the experimental precision and therapeutic effect.Specifically,ultra-large-area and high-uniformity scanning are crucial requirements for spacecraft radiation effects assessment and serve as core specification for beamline terminal design.In the 300 MeV proton and heavy ion accelerator complex at the Space Environment Simulation and Research Infrastructure(SESRI),proton and heavy ion beams will be accelerated and ultimately delivered to three irradiation terminals.In order to achieve the required large irradiation area of 320 mm×320 mm,horizontal and vertical scanning magnets are used in the extraction beam line.However,considering the various requirements for beam species and energies,the tracking accuracy of power supplies(PSs),the eddy current effect of scanning magnets,and the fluctuation of ion bunch structure will reduce the irradiation uniformity.To mitigate these effects,a beam uniformity optimization method based on the measured beam distribution was proposed and applied in the accelerator complex at SESRI.In the experiment,the uniformity is successfully optimized from 75%to over 90%after five iterations of adjustment to the PS waveforms.In this paper,the method and experimental results were introduced. 展开更多
关键词 heavy ion accelerator beam uniformity scanning magnet MIC
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Half-integer Shapiro steps in MgB_(2) focused He ion beam Josephson junctions
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作者 殷大利 蔡欣炜 +5 位作者 徐铁权 孙瑞宁 韩颖 张焱 王越 甘子钊 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第8期483-489,共7页
Half-integer microwave induced steps(Shapiro steps)have been observed in many different Josephson junction systems,which have attracted a lot of attention because they signify the deviation of current phase relation(C... Half-integer microwave induced steps(Shapiro steps)have been observed in many different Josephson junction systems,which have attracted a lot of attention because they signify the deviation of current phase relation(CPR)and uncover many unconventional physical properties.In this article,we first report the discovery of half-integer Shapiro steps in MgB_(2)focused He ion beam(He-FIB)Josephson junctions.The half-integer steps'dependence on microwave frequency,temperature,microwave power,and magnetic field is also analyzed.We find that the existence of half-integer steps can be controlled by the magnetic field periodically,which is similar to that of high temperature superconductor(HTS)grain boundary junctions,and the similarity of the microstructures between gain boundary junctions and He-FIB junctions is discussed.As a consequence,we mainly attribute the physical origin of half-integer steps in MgB_(2)He-FIB junctions to the model that a He-FIB junction is analogous to a parallel junctions'array.Our results show that He-FIB technology is a promising platform for researching CPR in junctions made of different superconductors. 展开更多
关键词 Josephson junction half-integer Shapiro steps MgB_(2) focused helium ion beam
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Screening of Biocontrol Strain Bacillus subtilis by N^+ Ion Beam Implantation 被引量:5
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作者 刘桂君 孟佑婷 +2 位作者 杨素玲 包放 尚宏忠 《Agricultural Science & Technology》 CAS 2012年第8期1658-1663,共6页
[Objective] This study was to investigate the effect of N+ ion beam implantation on the survival rate and mutation rate of biocontrol strain Bacillus subtilis. [Method] The factors influencing B. subtilis ion beam im... [Objective] This study was to investigate the effect of N+ ion beam implantation on the survival rate and mutation rate of biocontrol strain Bacillus subtilis. [Method] The factors influencing B. subtilis ion beam implantation, including culture time, dilution concentration, solvent, drying time of mycoderm were optimized. B. subtilis cells were implanted by using ion beam at dose of 2.0×10^14~4.0×10^14 ions/cm2 and the energy of 30 kev. Then the methods of culturing colonies confronting each other on plate and Oxford cup diffusion were used to screening strains. [Result] The optimal parameters were found as follows: culture in liquid for 20-24 h, dilution with sterile water to 106 cells/ml and drying time of 60 min for sample preparation; the optimal N+ ion beam implantation dose of 2.0×10^14~4.0×10^14 ions/cm2 at the energy of 30 kev, the survival rate of 8.43%-26.71% and the mutation rate of 3.50%-5.43%. [Conclusion] This study provided reference for ion beam implantation mutation of B. subtilis. 展开更多
关键词 ion beam implantation Survival rate Mutation rate Bacillus subtilis
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Gene Chip Analysis for Rice MicroRNA Response to Low Energy N^+ Beam Radiation
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作者 周晓君 刘旭昊 +1 位作者 郭向萌 押辉远 《Agricultural Science & Technology》 CAS 2010年第9期47-49,共3页
[Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N+ beam radiation in rice.[Method]Three groups of ion beam irradiation rice seeds and untreated rice seeds w... [Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N+ beam radiation in rice.[Method]Three groups of ion beam irradiation rice seeds and untreated rice seeds were selected respectively,and then the total RNA of seedlings after 96 h of germination was extracted at 30 ℃.Agilent gene chip was used to screen the differentially expressed genes of two groups of rice seedlings.The chip contained 510 miRNA probes;and about two times of expression differences between two samples were considered as the threshold range.[Result]14 miRNA molecules showed significant expression differences between the irradiated group and the control group,and all of them were decreased.[Conclusion]The miRNAs showed expression differences between irradiated group and the control group,which might regulate the expression of certain genes to respond to ion irradiation,thus producing physiological,biochemical and phenotypic differences. 展开更多
关键词 Nitrogen ion beam Irradiated rice MiRNA Differential expression Gene chip
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Preliminary Studies on Base Substitutions and Repair of DNA Mismatch Damage Stimulated by Low Energy N^+ Ion Beam Implantation in Escherichia coli 被引量:4
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作者 谢传晓 郭金华 +1 位作者 程备久 余增亮 《Plasma Science and Technology》 SCIE EI CAS CSCD 2003年第1期1677-1682,共6页
Ever since the low energy N+ ion beam has been accepted that the mutation effects of ionizing radiation are attributed mainly to direct or indirect damage to DNA. Evidences based on naked DNA irradiation in support of... Ever since the low energy N+ ion beam has been accepted that the mutation effects of ionizing radiation are attributed mainly to direct or indirect damage to DNA. Evidences based on naked DNA irradiation in support of a mutation spectrum appears to be consistent, but direct proof of such results in vivo are limited. Using mutS, dam and/or dcm defective Eschericha coli imitator strains, an preliminary experimental system on induction of in vivo mutation spectra of low energy N+ ion beam has been established in this study. It was observed that the mutation rates of rifampicin resistance induced by N+ implantation were quite high, ranging from 9.2 x 10~8 to 4.9× 10~5 at the dosage of 5.2×1014 ions/cm2. Strains all had more than 90-fold higher mutation rate than its spontaneous mutation rate determined by this method. It reveals that base substitutions involve in induction of mutation of low energy nitrogen ion beam implantation. The mutation rates of mutator strains were nearly 500-fold (GM2929), 400-fold (GM5864) and 6-fold larger than that of AB1157. The GM2929 and GM5864 both lose the ability of repair DNA mismatch damage by virtue of both dam and dcm pathways defective (GM2929) or failing to assemble the repair complex (GM5864) respectively. It may explain the both strains had a similar higher mutation rate than GM124 did. It indicated that DNA cytosine methylase might play an important role in mismatch repair of DNA damage induced by N+ implantation. The further related research were also discussed. 展开更多
关键词 low energy N^+ ion beam base substitutions dam(DNA adenine methylase) dcm(DNA cytosine methylase) MUTS MMR (mismatch repair) Escherichia coli mutator strain
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Lifetime measurement of the 3d^(9) ^(2)D_(3/2) metastable level in Mo^(15+)at an electron beam ion trap
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作者 刘佳林 王银涛 +7 位作者 屠秉晟 黄良玉 司然 李冀光 张明武 傅云清 邹亚明 姚科 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第10期400-404,共5页
An experimental measurement of the lifetime of 3d^(9) ^(2)D_(3/2) metastable level in Mo^(15+)is reported in this work.The Mo^(15+)ions are produced and trapped in an electron beam ion trap with a magnetic field of 0.... An experimental measurement of the lifetime of 3d^(9) ^(2)D_(3/2) metastable level in Mo^(15+)is reported in this work.The Mo^(15+)ions are produced and trapped in an electron beam ion trap with a magnetic field of 0.65 T.The decay photons emitted from 3d^(9) ^(2)D_(3/2) level are subsequently recorded via a cooled photomultiplier tube.Through meticulous scrutiny of potential systematic uncertainties affecting the measurement outcomes,we have determined the lifetime of Mo^(15+)3d^(9)2D_(3/2)metastable level to be 2.83(22)ms.The experimental result provides a clear distinguishment from existing calculations based on various theoretical approaches. 展开更多
关键词 lifetime measurement molybdenum ions metastable levels electron beam ion trap(EBIT)
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Fuel compression in the magnetized cylindrical implosion driven by a gold tube heated by heavy ion beams
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作者 刘博 王芳平 +2 位作者 张恒 张晟 段文山 《Plasma Science and Technology》 SCIE EI CAS CSCD 2023年第4期61-68,共8页
A magnetized cylindrical target composed of a gold tube filled with deuterium-tritium fuel plasma at low density is studied numerically in the present paper.A shock wave is produced when a heavy ion beam heats the gol... A magnetized cylindrical target composed of a gold tube filled with deuterium-tritium fuel plasma at low density is studied numerically in the present paper.A shock wave is produced when a heavy ion beam heats the gold along the direction of the magnetic field.The density peak of the shock wave increases with the increase in time and it propagates in the-r direction in the cylindrical tube.It seems that this wave is the supermagnetosonic wave.It is found that the Mach number M is between 6.96 and 19.19.The density peak of the shock wave increases as the intensity of the heavy ion beam increases.Furthermore,the density peak of the shock wave increases as the external magnetic field increases. 展开更多
关键词 high energy density heavy ions beam magnetohydrodynamic(MHD)simulation
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Biological Effects on Fruit Fly by N^+ ion Beam Implantation 被引量:1
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作者 尹若春 《High Technology Letters》 EI CAS 2001年第3期5-7,共3页
Mutation induced by low energy ion beam implantation has been applied widely both in plants and microbes. However, due to the vacuum limitation, such ion implantation into animals was never studied except for silkworm... Mutation induced by low energy ion beam implantation has been applied widely both in plants and microbes. However, due to the vacuum limitation, such ion implantation into animals was never studied except for silkworm. In this study, Pupae of fruit fly were irradiated with different dosage N+ ions at energy 20 KeV to study the biological effect of ion beam on animal. The results showed a saddle like curve exists between incubate rate and dosage. Damage of pupae by ion beam implantation was observed using scanning electron microscope. Some individuals with incomplete wing were obtained after implantation but no similar character was observed in their offspring. Furthermore, about 5.47% mutants with wide variation appeared in M1 generation. Therefore, ion beam implantation could be widely used for mutation breeding. 展开更多
关键词 ion beam IMPLANTATion FRUIT FLY ( Drosophilae MELANOGASTER ) MUTATion
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Influence of extraction voltage on electron and ion behavior characteristics
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作者 徐翱 甘娉娉 +1 位作者 石元杰 陈磊 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第4期553-559,共7页
The characteristics of the extracted ion current have a significant impact on the design and testing of ion source performance.In this paper,a 2D in space and 3D in velocity space particle in cell(2D3V PIC)method is u... The characteristics of the extracted ion current have a significant impact on the design and testing of ion source performance.In this paper,a 2D in space and 3D in velocity space particle in cell(2D3V PIC)method is utilized to simulate plasma motion and ion extraction characteristics under various initial plasma velocity distributions and extraction voltages in a Cartesian coordinate system.The plasma density is of the order of 10^(15)m^(-3)-10^(16)m^(-3)and the extraction voltage is of the order of 100 V-1000 V.The study investigates the impact of various extraction voltages on the velocity and density distributions of electrons and positive ions,and analyzes the influence of different initial plasma velocity distributions on the extraction current.The simulation results reveal that the main reason for the variation of extraction current is the spacecharge force formed by the relative aggregation of positive and negative net charges.This lays the foundation for a deeper understanding of extraction beam characteristics. 展开更多
关键词 extraction voltage PLASMA ion beam ion current
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Experimental results of a magnetic field modification to the radio frequency driver of a negative ion source
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作者 谢俊炜 谢亚红 +6 位作者 韦江龙 梁立振 彭旭峰 杨宇雯 顾玉明 胡纯栋 谢远来 《Plasma Science and Technology》 SCIE EI CAS CSCD 2024年第4期162-167,共6页
A magnetic field produced by a current flowing through the plasma grid(PG) is one of the solutions to reduce the collisional loss of negative ions in a negative ion source, which reduces the electron temperature in fr... A magnetic field produced by a current flowing through the plasma grid(PG) is one of the solutions to reduce the collisional loss of negative ions in a negative ion source, which reduces the electron temperature in front of the PG. However, the magnetic field diffused into the driver has some influence on the plasma outflowing. In order to investigate the effect of changing this magnetic field on the outflowing of plasma from the driver, a circular ring(absorber) of high permeability iron has been introduced at the driver exit, which can reduce the magnetic field around it and improve plasma outflowing. With the application of the absorber, the electron density is increased by about 35%, and the extraction current measured from the extraction grid is increased from 1.02 A to 1.29 A. The results of the extraction experiment with cesium injection show that both the extraction grid(EG) current and H-current are increased when the absorber is introduced. 展开更多
关键词 neutral beam injection negative ion source magnetic filter radio frequency ion source
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Impact of T_(i)/T_(e )ratio on ion transport based on EAST H-mode plasmas
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作者 刘建文 臧庆 +14 位作者 梁云峰 陈佳乐 邬潇河 Alexander KNIEPS 扈嘉辉 金仡飞 张斌 储宇奇 刘海庆 吕波 段艳敏 李妙辉 陈颖杰 龚先祖 the EAST Team 《Plasma Science and Technology》 SCIE EI CAS CSCD 2024年第4期68-78,共11页
At the EAST tokamak, the ion temperature(T_(i)) is observed to be clamped around 1.25 keV in electron cyclotron resonance(ECR)-heated plasmas, even at core electron temperatures up to 10 keV(depending on the ECR heati... At the EAST tokamak, the ion temperature(T_(i)) is observed to be clamped around 1.25 keV in electron cyclotron resonance(ECR)-heated plasmas, even at core electron temperatures up to 10 keV(depending on the ECR heating power and the plasma density). This clamping results from the lack of direct ion heating and high levels of turbulence-driven transport. Turbulent transport analysis shows that trapped electron mode and electron temperature gradient-driven modes are the most unstable modes in the core of ECR-heated H-mode plasmas. Nevertheless, recently it was found that the T_(i)/T_(e)ratio can increase further with the fraction of the neutral beam injection(NBI) power, which leads to a higher core ion temperature(Ti0). In NBI heating-dominant H-mode plasmas, the ion temperature gradient-driven modes become the most unstable modes.Furthermore, a strong and broad internal transport barrier(ITB) can form at the plasma core in high-power NBI-heated H-mode plasmas when the T_(i)/T_(e)ratio approaches ~1, which results in steep core Teand Tiprofiles, as well as a peaked neprofile. Power balance analysis shows a weaker Teprofile stiffness after the formation of ITBs in the core plasma region, where Ticlamping is broken,and the core Tican increase further above 2 keV, which is 80% higher than the value of Ticlamping in ECR-heated plasmas. This finding proposes a possible solution to the problem of Ticlamping on EAST and demonstrates an advanced operational regime with the formation of a strong and broad ITB for future fusion plasmas dominated by electron heating. 展开更多
关键词 ion temperature clamping TRANSPORT neutral beam injection STIFFNESS internal transport barrier
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In situ luminescence measurements of GaN/Al_(2)O_(3) film under different energy proton irradiations
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作者 蒋文丽 欧阳潇 +6 位作者 仇猛淋 英敏菊 陈琳 庞盼 张春雷 张耀锋 廖斌 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第5期704-710,共7页
Ion beam-induced luminescence(IBIL) experiments were performed to investigate the in situ luminescence of GaN/Al_(2)O_(3) at varying ion energies,which allowed for the measurement of defects at different depths within... Ion beam-induced luminescence(IBIL) experiments were performed to investigate the in situ luminescence of GaN/Al_(2)O_(3) at varying ion energies,which allowed for the measurement of defects at different depths within the material.The energies of H^(+)were set to 500 keV,640 keV and 2 MeV,the Bragg peaks of which correspond to the GaN film,GaN/Al_(2)O_(3) heterojunction and Al_(2)O_(3) substrate,respectively.A photoluminescence measurement at 250 K was also performed for comparison,during which only near band edge(NBE) and yellow band luminescence in the GaN film were observed.The evolution of the luminescence of the NBE and yellow band in the GaN film was discussed,and both exhibited a decrease with the fluence of H^(+).Additionally,the luminescence of F centers,induced by oxygen vacancies,and Cr^(3+),resulting from the ^(2)E →^(4)A_(2) radiative transition in Al_(2)O_(3),were measured using 2 MeV H^(+).The luminescence intensity of F centers increases gradually with the fluence of H^(+).The luminescence evolution of Cr^(3+)is consistent with a yellow band center,attributed to its weak intensity,and it is situated within the emission band of the yellow band in the GaN film.Our results show that IBIL measurement can effectively detect the luminescence behavior of multilayer films by adjusting the ion energy.Luminescence measurement can be excited by various techniques,but IBIL can satisfy in situ luminescence measurement,and multilayer structural materials of tens of micrometers can be measured through IBIL by adjusting the energy of the inducing ions.The evolution of defects at different layers with ion fluence can be obtained. 展开更多
关键词 ion beam-induced luminescence(IBIL) GaN/Al_(2)O_(3) ion beam
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Biological Effects of Low Energy N^+ Beams Implantation on Calluses of Autotetraploid Rice
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作者 ZHAO Shuai-peng HUANG Qun-ce +3 位作者 LIANG Qiu-xia ZHANG Shu-gen JIAO Zhen HUANG Wei-zhou 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2013年第11期2045-2055,共11页
Calluses of two autotetraploid rice (DPR (4) and Zijing (4)) were implanted with 30 KeV N+beams at doses ranging from 1.0×1015 to 9.0×1015 ions cm-2. The differentiation rates of calluses, the concent... Calluses of two autotetraploid rice (DPR (4) and Zijing (4)) were implanted with 30 KeV N+beams at doses ranging from 1.0×1015 to 9.0×1015 ions cm-2. The differentiation rates of calluses, the concentration of malondialdehyde (MDA), the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD), and the change of esterase (EST) spectrum were measured and analyzed on the calluses stage. The results showed that differentiation rates of calluses under 3.0×1015 ions cm-2 ((46.12±2.01)%) were higher than the other treatment groups in DPR (4), and those of Zijing (4) were similar under the doses of 1.0×1015, 3.0×1015 and 5.0×1015 ions cm-2 ((40.55±2.74), (37.77±3.04) and (34.56±2.65)%) and were higher than that of 7.0×1015 and 9.0×1015 ions cm-2. When the doses were 3.0×1015 and 5.0×1015 ions cm-2, the activities of SOD, POD and CAT were notably enhanced, and the accumulation of MDA content was markedly alleviated in the implanted calluses of two materials, as well as the activity of EST was increased by the additional isforms DPR (4). Whereas the anti-oxidative systems and enzymes spectrum of EST were destroyed seriously, the accumulation of MDA was signiifcantly aggravated, also the differentiation rate of calluses reduced sharply and almost closed to zero when the implantation doses were 7.0×1015 and 9.0×1015 ions cm-2. It suggested that the proper dose of ion implantation effectively maintained the normal membrane structure and metabolism, and it would be also a feasible approach for autotetraploid rice to improve its genetic characteristics at calluses stage. Additionally, the differences of the radiation resistance between DPR (4) and Zijing (4) could be related to the different genotypes. 展开更多
关键词 ion beams peroxidase (POD) superoxide dismutase (SOD) catalase (CAT) esterase (EST) malondialdehyde(MDA) autotetraploid rice
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Study of Genetics and Embryology of Polyembryonic Mutant of Autotetraploid Rice Induced by N^+ Beam Implantation
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作者 代西梅 黄群策 +3 位作者 李国平 胡秀明 秦广雍 余增亮 《Plasma Science and Technology》 SCIE EI CAS CSCD 2006年第6期745-750,共6页
In the present study autotetraploid rice IR36-4X was treated by an ion implantation technique with nitrogen ion beams. A polyembryonic mutant (named IR36-Shuang) was identified in the M2 generation. The mutant line ... In the present study autotetraploid rice IR36-4X was treated by an ion implantation technique with nitrogen ion beams. A polyembryonic mutant (named IR36-Shuang) was identified in the M2 generation. The mutant line and its offspring were systematically investigated in regard to their major agronomic properties and the rate of polyembryonic seedling in the M3-M6 generation. The abnormal phenomena in the embryo sac development and the cytological mechanism of the initiation of additional embryo in IR36-Shuang were observed by Laser Scanning Confocal Microscopy. The results were as follows. 1) The plant height, the panicle length and 1000 grain weight of IRB6-Shuang were lower than that of its control by 35.41%, 5.08% and 15.72% respectively, Moreover, the setting percentage decreased 12.39% compared with that in normal IRB6-4X plants. 2) The polyembryonic trait of IRB6-Shuang was genetically stable and the frequency of the polyembryonic seedlings in the IR36-Shuang line was also relatively stable. 3) The rate of abnormal embryo sacs in IR36-Shuang was significantly higher than that in the control IR36-4X. 4) The additional embryo in IR36-Shuang might arise from the double Jet of embryo sacs in a single owry, antipodal cells or endosperm cells. These results suggest that IRB6-Shuang is a polyembryonic mutant and a new apomixis rice line induced by low energy ion implantation. The prospects for the application in production of the IR36-Shuang line are also discussed. The present study may provide a basis for future investigations of apomixis rice breeding via the ion implantation biotechnology. 展开更多
关键词 ion beam implantation mutant polyembryonic seedling autotetraploid rice embrvogenesis
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Breeding of High-Yield Salinomycin-Producing Streptomyces albus Strains by Low Energy N^+ Ion Beam Irradiation
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作者 Liqin WU Fengxiang MIAO +1 位作者 Haike GU Hongzhong SHANG 《Agricultural Biotechnology》 2012年第5期55-56,F0003,共3页
[Objective] This study aimed to explore the mutagenesis effects of N+ ion beam implantation on Streptomyces a/bus and obtain high-yield salinomycin- producing mutant strain. [ Method ] Streptomyces a/bus strain S-11-... [Objective] This study aimed to explore the mutagenesis effects of N+ ion beam implantation on Streptomyces a/bus and obtain high-yield salinomycin- producing mutant strain. [ Method ] Streptomyces a/bus strain S-11-04 was mutated with different doses of N + implantation. The effects of low energy N * implantation on the survival rate, colony morphology and salinomycin-producing ability were investigated. [ Result] The results showed that low energy N + implantation can efficiently improve the positive mutation rate of Streptomyces albus; 13 mutant strains with high yield of salinomycin were isolated; to be specific, mutant strain N3- 6 has relatively good genetic stability with four continuous generations, and the titres of salinomycin were increased by 41% in the shake-flask culture and 20.5% in mass production compared with the control. [ Conclusion ] N + ion beam irradiation is an effective method to obtain high-yield salinomycin-producing Streptomy- ces albus strain. 展开更多
关键词 Streptomyces albus N ion beam irradiation SALINOMYCIN Mutation breeding
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Evidence for Base Substitutions and Repair of DNA Mismatch Damage Induced by Low Energy N^+ Ion Beam Implantation in E. coli
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作者 谢传晓 Guo +6 位作者 Jinhua Yao Jianmin Wu Lijun Yu Zengliang 《High Technology Letters》 EI CAS 2003年第2期1-6,共6页
Ever since the low energy N + ion beam has been accepted, the mutations of ionizing radiation are attributable mainly to avoidance of DNA damages repair. Evidences based on in vivo proof results are limited. Using the... Ever since the low energy N + ion beam has been accepted, the mutations of ionizing radiation are attributable mainly to avoidance of DNA damages repair. Evidences based on in vivo proof results are limited. Using the E.coli wild type and mutator strains, the mutant frequencies suggest that base substitutions in rpoB gene are induced by the N + implantation. A highly conserved region is selected to get the direct evidence for base substitutions by sequence of the high fidelity PCR amplification products in mutants. Most of the mutants (90.9%, 40/44) have at least one base substitution in the amplification region. The evidences for CG to TA (55%, 22/40), AT to GC (20%, 8/40) and TA to CG (5%, 2/40) transitions are identified. The transversions are AT to TA (15%, 6/40) and GC to CG (5%, 2/40). It is suggested that DNA cytosine methylase might play an important role in mismatch repair of DNA damage induced by N + implantation by analysis of the mutant frequencies of mutator strains. 展开更多
关键词 low energy N + ion beam base substitutions mismatch repair mutator strains
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Preliminary Study on the Low Energy Ion Beam Mediated Parameters with Fractional Factorial Design Method 被引量:2
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作者 陈雪能 黄群策 赵帅鹏 《Agricultural Science & Technology》 CAS 2010年第8期137-139,共3页
[Objective] The aim was to explore the biological effect of low energy ion beam mediated parameters with fractional factorial design method. [Method] The twin-embryos seed of autotetraploid rice DER10-04-01 was taken ... [Objective] The aim was to explore the biological effect of low energy ion beam mediated parameters with fractional factorial design method. [Method] The twin-embryos seed of autotetraploid rice DER10-04-01 was taken as the receptor material,and the Elymus dahuricus Turcz. was used as materials to provide DNA to carry out the ion beam mediated experiment. And the fractional factorial design method was used to study the parameters of low enery N+ ion beam mediated foreign genes into rice. [Result] The implantation energy,dose,DNA concentrations and immersion time of DNA showed significant biological effects on the normal growth and development of DER10-04-01,in which the biological effects of implantation dose and DNA concentrations were relatively obvious. [Conclusion] The implantation energy,dose,DNA concentrations and immersion time of DNA were major factors showing important effects on the experimental result in ion beam mediated foreign genetic materials. 展开更多
关键词 ion beam Factorial experiment design Rice with twin-embryos seedlings Elymus dahuricus Turcz.
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High-Power and High-Efficiency 650nm-Band AlGaInP Visible Laser Diodes Fabricated by Ion Beam Etching
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作者 徐云 曹青 +4 位作者 孙永伟 叶晓军 侯识华 郭良 陈良惠 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2004年第9期1079-1083,共5页
High power and high-slope efficiency 650nm band real-refractive-index ridge w aveguide AlGaInP laser diodes with compressive strained MQW active layer are for med by pure Ar ion beam etching process.Symmetric laser me... High power and high-slope efficiency 650nm band real-refractive-index ridge w aveguide AlGaInP laser diodes with compressive strained MQW active layer are for med by pure Ar ion beam etching process.Symmetric laser mesas with high perpendi cularity,which are impossible to obtain by traditional wet etching method due to the use of a 15°-misoriented substrate,are obtained by this dry etching metho d.Laser diodes with 4μm wide,600μm long and 10%/90% coat are fabricated.Th e typical threshold current of these devices is 46mA at room temperature,and a s table fundamental-mode operation over 40mW is obtained.Very high slope efficien cy of 1.4W/A at 10mW and 1.1W/A at 40mW are realized. 展开更多
关键词 AlGaInP visible lasers Ar ion beam dry etching
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Effects of Heavy-ion Beams Irradiation on Survival Rate and Antioxidant Enzymes of Sweet Sorghum Seedlings
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作者 刘智全 谷卫彬 李文建 《Agricultural Science & Technology》 CAS 2012年第11期2257-2260,2268,共5页
[Objective] This study aimed to investigate the effects of heavy-ion beams irradiation on the seed germination potential, survival rate, antioxidant enzyme activi- ties and lipid peroxidation of sweet sorghum. [Method... [Objective] This study aimed to investigate the effects of heavy-ion beams irradiation on the seed germination potential, survival rate, antioxidant enzyme activi- ties and lipid peroxidation of sweet sorghum. [Method] The dry seeds were irradiated by '2(36. heavy ion beams with absorbed doses: 0, 40, 80, 120, 160 and 200 Gy, respectively. Then, the seed germination potential, survival rate, antioxidant enzyme activities and lipid peroxidation of sweet sorghum were measured. [Result] Heavy-ion beams irradiation exhibited different influence on germination potential and survival rates. Germination rate showed a downward trend, but the corresponding survival curve of seedlings was saddle-shaped. The activities of SOD, POD, CAT and ASA- POD changed in different trends as well. The MDA content rose toward increasing irradiation dose, suggesting that high dose of heavy-ion beams irradiation enhanced the damage to membrane of sweet sorghum seedlings. [Conclusion] After being irra- diated, germination potential and survival rates of sweet sorghum were decreased, and antioxidant enzymes activity changed greatly. This study laid the basis for fur- ther work on breeding and improvement of sweet sorghum irradiated by ,^(12)C^(6+) heavy ion beams. 展开更多
关键词 Heavy ion beams IRRADIATion Sweet sorghum Antioxidant enzymes
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