Effects of Regulation of C/N Ratio Wheat Straw Application on Nitrogen,Phosphorus and Potassium Uptake in Tobacco

[Objective]The aim was to study the effects of regulation of C/N ratio wheat straw application on tobacco nitrogen,phosphorus and potassium uptake. [Method]Effects of regulation C/N ratio wheat straw application on the flue-cured tobacco yield,output value,nitrogen,phosphorus and potassium content and cumulative uptake of the upper,middle and bottom leaf were studied by using the field plot experiments at Banqiao town,Qujing city,Yunnan Province during the 2008-2009 summer growing seasons. [Result]The results showed that the application of wheat straw alone or after C/N regulation,could significantly increase tobacco production,potassium content,the potassium and nitrogen accumulation amount of leaf,and was more conducive to the potassium uptake of tobacco leaf with wheat straw application after C/N regulation. Compared with non-straw application,the yield of tobacco increased by 6.59%,3.58%,5.98%,8.80% with application of wheat straw alone,wheat straw and vetch,wheat straw and oilseed cake,wheat straw and urea nitrogen,the potassium content in tobacco leaf increased by 3.85%,7.76%,8.82%,11.21%,respectively,the total potassium cumulative amount of leaf increased by 10.71%,11.62%,15.32% ,21.01% and the total nitrogen cumulative amount increased by 9.76%,1.22%,8.14%,14.00%. However,the differences of tobacco leaf nitrogen content among the different treatments were not significant,the phosphorus uptake of tobacco leaf decreased. [Conclusion]application of high C/N ratio wheat straw in flue-cured tobacco production,which should be concerned not only to adjust C/N ratio by adding nitrogen,but also considering additional phosphorus application.

Effects of Amino Acids Replacing Nitrate on Growth,Nitrate Accumulation,and Macroelement Concentrations in Pak-choi (Brassica chinensis L.)

A hydroponic experiment was carried out to determine the influence of replacing 20% of nitrate-N in nutrient solutions with 20 individual amino acids on growth, nitrate accumulation, and concentrations of nitrogen （N）, phosphorus （P）, and potassium （K） in pak-choi （Brassica chinensis L.） shoots. When 20% of nitrate-N was replaced with arginine （Arg） compared to the full nitrate treatment, pak-choi shoot fresh and dry weights increased significantly （P ≤ 0.05）, but when 20% of nitrate-N was replaced with alanine （Ala）, valine （Val）, leucine （Leu）, isoleucine （Ile）, proline （Pro）, phenylalanine （Phe）, methionine （Met）, aspartic acid （Asp）, glutamic acid （Glu）, lysine （Lys）, glycine （Gly）, serine （Ser）, threonine （Thr）, cysteine （Cys）, and tyrosine （Tyr）, shoot fresh and dry weights decreased significantly （P ≤ 0.05）. After replacing 20% of nitrate-N with asparagine （Asn） and glutamine （Gin）, shoot fresh and dry weights were unaffected. Compared to the full nitrate treatment, amino acid replacement treatments, except for Cys, Gly, histidine （His）, and Arg, significantly reduced （P ≤0.05） nitrate concentrations in plant shoots. Except for Cys, Leu, Pro, and Met, total N concentrations in plant tissues of the other amino acid treatments significantly increased （P ≤ 0.05）. Amino acids also affected total P and K concentrations, but the effects differed depending on individual amino acids. To improve pak-choi shoot quality, Gln and Asn, due to their insignificant effects on pak-choi growth, their significant reduction in nitrate concentrations, and their increase in macroelement content in plants, may be used to partially replace nitrate-N.

Effects of ambient DIN:DIP ratio on the nitrogen uptake of harmful dinoflagellate Prorocentrum minimum and Prorocentrum donghaiense in turbidistat

The effects of varying nitrogen （N）： phosphorus （P） ratios on the growth and N-uptake and assimilation of the harmful dinoflagellates Prorocentrum minimum and Prorocentrum donghaiense were examined in turbidistat culture experiments. Algal cultures were supplied with media containing PO4^3- in various concentrations to obtain a wide range of N：P ratios. Experiments to determine rates of N uptake and assimilation of different N sources （NO^3-, NH4^＋, urea and glycine by P. minimum and NO3^-, NH4^＋ by P. donghaiense） were conducted using ^15-N tracer techniques at each N：P ratio. The growth rates suggested nutrient limitation at both high and low N：P ratios relative to the Redfield ratio. On a diel basis, the growth of both species was regulated by the light-dark cycle, which may be a result of regulation of both lightdependent growth and light-independent nutrient uptake. Maximum growth rates of both species always occurred at the beginning of light phase. In P-rich medium （low N：P ratio）, both species had higher N assimilation rates, suggesting N limitation. Low assimilation coefficients at high N：P ratios suggested P limitation of N uptake and assimilation. NO3 ^-and NH4^＋ contributed more than 90% of the total N uptake of P. minimum. Reduced N sources were more quickly assimilated than NO3^-. Highest average daily growth rates were recorded near an N：P ratio of 12 for both species. The N uptake rates of cultures at N：P ratios near Redfield ratio were more balanced with growth rates. The linkage between growth rates and N uptake/assimilation rates were conceptually described by the variation of cell N quota. The N：P ratios affect the N uptake and growth of Prorocentrum spp., and may regulate their bloom progression in eutrophic ecosystems.

Variation of Bioaccumulation Ability of 2,2',4,4'-Tetrabromodiphenyl Ether by Marine Diatom Skeletonema costatum Under Different N:P Ratios

The growth, biochemical content and bioaccumulation quantity of 2,2',4,4'-tetrabromodiphenyl ether(BDE-47) in Skeletonema costatum were studied under different N:P ratios(1, 4, 16, 64 and 128). All cellular biochemical contents of S. costatum presented decreasing trend over cultivation time. At early stage of cultivation, the cellular protein, carbohydrate and lipid in S. costatum presented higher values in treatments of N:P=4 and 16. However, they were lower in these treatments at the late stage, but higher in treatments N:P=1 and 128. Similarly, BDE-47 levels per cell of S. costatum were higher in treatments of N:P=4 and 16 at early stage of cultivation, which were 3.8 and 3.7 ng(106 cells)-1, respectively. At the middle stage of cultivation, the BDE-47 level per S. costatum cell lowered; and it further reduced in the treatments of N:P=4 and 16 at the late stage with the values 0.6 and 0.5 ng(106 cells)-1, respectively. However, it rose in N:P=128, reaching up to 2.3 ng(106 cells)-1. Compared with BDE-47 per cell, BDE-47 per algal volume under different N:P ratios did not present obvious difference. The quantity BDE-47 accumulated per cell of S. costatum was positively correlated with protein, carbohydrate and lipid per cell; meanwhile, the BDE-47 per volume had a positive correlation with biochemical content per volume. The variation of bioaccumulation ability of BDE-47 in S. costatum can be explained by biochemical changes due to N:P ratios.

The Inhibitory Degree Between Skeletonema costatum and Dinoflagllate Prorocentrum donghaiense at Different Concentrations of Phosphate and Nitrate/Phosphate Ratios

Interactions between Skeletonema costatum (S. costatum) and Prorocentrum donghaiense (P. donghaiiense) were inves-tigated using bi-algal cultures at different concentrations of phosphate (PO4-P) and nitrate/phosphate (N/P) ratios. Experiments were conducted under P-limited conditions and the Lotka-Volterra mathematical model was used to simulate the growth of S. costatum and P. donghaiense in the bi-algal cultures. Both of these two species were inhibited significantly in bi-algal culture. The results of the simulation showed that the inhibitory degree of S. costaum by P. donghaiense was high when the concentration of PO4-P was low (0.1μmolL-1/2 d), but that of P. donghaiense by S. costaum was high with increased PO4-P supply (0.6μmolL-1/2 d). At low concen-tration of PO4-P (0.1μmolL-1/2 d), or high concentration of PO4-P (0.6μmolL-1/2 d) with high N/P ratio (160), the interactions be-tween S. costatum and P. donghaiense were dependent on the initial cell densities of both species. At high concentration of PO4-P (0.6μmolL-1/2 d) with low N/P ratio (25 or 80), S. costatum exhibited a survival strategy superior to that of P. donghaiense. The de-gree of inhibition of P. donghaiense by S. costaum increased with elevated N/P ratio when the medium was supplemented with con-centration 0.1μmolL-1/2 d of PO4-P. The degree of inhibition to P. donghaiense by S. costaum increased with elevated N/P ratio at low concentration of PO4-P (0.1 μmolL-1/2 d). This trend was conversed at high concentration of PO4-P (0.6μmolL-1/2 d). However, the degree of inhibition of S. costaum by P. donghaiense increased with the increased N/P ratio at different PO4-P concentrations (0.1μmolL-1/2 d and 0.6μmolL-1/2 d). These results suggested that both phosphate concentration and N/P ratio affected the competition between S. costaum and P. donghaiense: P. donghaiense is more competitive in environments with low phosphate or high N/P ratio and the influence of N/P ratio on the competition was more significant with lower phosphate concentration.

藻类爆发对太湖梅梁湾水体中NPK含量的影响

1998～ 2 0 0 0年对太湖梅梁湾的实测结果表明 ,在一定范围内叶绿素a的含量与温度呈现明显的正相关 ;NH4+ N和总氮 (TN)、总磷 (TP)含量在每年的八九月份最低 ,总含量与叶绿素a呈正相关 ,随着叶绿素a含量上升 ,N、P含量呈现下降趋势 ;K的含量与N、P相似 .排污口的 0号点和 6号点的N、P含量相对于湖中心的 8号样点来说极高 ,而且P已不是限制性营养元素 ,在其它水域 ,P依然是限制性营养元素 ,表明微囊藻 (Microcystisspp .)只在TN含量为 1～ 4mg·L-1、TP在 0 .1～ 0 .

N、P、K营养元素不同配比对马占相思苗期生长的影响

采用N、P、K 3营养元素 5个水平配比试验 ,分析不同配比对马占相思苗期生长的影响 ;通过正交试验 ,确定马占相思苗木对各营养元素的实际需求水平。结果表明 ,P素对马占相思苗期生长影响最大 ,其次为K素 ,而N素的影响相对较小。不同水平的N、P、K配比对试验苗有不同的影响 ,其中 ,N∶P∶K最佳配比为 :190∶2 5∶16 0 (mg·L- 1)。

N、P、K肥对香根草修复土壤镉、锌污染效率的影响

通过盆栽试验研究在30 mg/kg镉(Cd)污染土壤条件下N[CO(NH2)2:100、200、300 mg/kg土]、P(P2O5:50、100、200 mg/kg土)和K(KCl:100、200、300 mg/kg土)处理对香根草修复土壤Cd和锌(Zn)污染效率的影响。结果表明:3种N处理能促进香根草地上部生长,而且显著提高地上部特别是叶的Cd和Zn含量,导致其修复效率成倍显著增加;200 mg/kg K处理显著提高Zn修复效率,但300 mg/kg K和50、200 mg/kg P处理却显著降低Cd、Zn修复效率。因此,为改善香根草对较贫瘠土壤中Cd、Zn污染的修复效率,应对香根草适施N肥,并控制或者不施P、K肥为佳。

不同生育时期根际土壤渍水逆境对冬小麦N、P、K素营养的影响

运用盆栽模拟土壤渍水逆境试验,研究不同生育时期根际土壤渍水逆境对不同小麦品种N、P、K素吸收、运转和分配的影响。结果表明,根际土壤渍水逆境对不同小麦品种N、P、K素吸收的影响有异;不同生育时期根际土壤渍水逆境显著影响根系对N、P素的吸收、运转与分配,以孕穗期渍水逆境影响最大,其次为灌浆期和拔节期,而不同生育时期根际土壤渍水逆境对K素的吸收影响较小。孕穗期以前渍水逆境主要影响小麦根系对N、P、K素的吸收,对N、P、K素在小麦体内的运输和分配影响较小;灌浆期渍水逆境不仅影响根系对N、P、K素的吸收,同时也影响N、P素在地上部各器官中的运转和分配,但对K素在小麦体内的运转和分配影响较小。因此,基肥中施足P肥和K肥,拔节孕穗期重施速效N肥,灌浆期叶面喷施KH2PO4对于培育壮秆大穗,减轻小麦渍害,提高受渍小麦籽粒产量具有非常重要的实际意义。

莲雾遮光期叶片SPAD值和C、N、P、K含量的变化及其规律

对大田莲雾成年树进行遮光处理,研究遮光叶片SPAD值及C、N、P、K含量的动态变化及其规律。结果表明:叶片SPAD值受叶龄和遮光互作影响,自然条件下,叶龄40 d左右SPAD值最大,达到63.0±2.49。短时间遮光对叶片SPAD值的影响不大,遮光40 d叶片SPAD值降到最低;长时间遮光会造成叶片早衰,限制树体营养生长。遮光对叶片N、P、K、C含量均有影响,在遮光过程中,叶片的C、N、P、K含量的变化情况不尽相同,但树体对遮光后形成的弱光条件均作出响应,然后通过自身的调节,慢慢使C、N、P、K趋同于自然条件下的含量水平。莲雾叶片的碳氮比(C/N)随着遮光时间的延长而呈增大趋势,遮光40 d左右比值达到顶峰,此时是莲雾栽培上进行催花的一个关键时间点。

长江河口九段沙互花米草湿地生态系统N、P、K的循环特征

研究了九段沙外来入侵种互花米草(Spartina alterniflora)湿地生态系统的营养元素含量、分布规律与循环特征。结果表明,九段沙的上沙、中沙和下沙互花米草湿地土壤全量养分含量差异相对较小,而速效性养分含量差异相对较大。土壤剖面中TK含量大大高于TN和TP含量,排序为:TK>TN>TP。各沙洲速效性养分含量排序为:速效K>速效N>速效P。土壤速效性养分与全量养分的空间分布规律并不一致,土壤剖面营养元素的垂直分布差异比较明显,速效性养分土壤剖面垂直分异比全量养分显著。各深度土壤营养元素含量均存在差异,不同沙洲同一深度土壤营养元素含量也存在差异。各沙洲不同深度土壤TN含量的差异明显大于TP和TK含量差异。植物中3种营养元素含量以K最高,N其次,P最低。湿地生态系统营养元素归还量远大于存留量。吸收系数排序为:N>P>K。不同沙洲营养元素的利用系数和循环系数存在明显差异,上沙P元素、中沙与下沙K元素利用系数最大,上沙K元素、中沙P元素与下沙N元素循环系数最大。

施硒对苦荞N,P,K营养元素和土壤有效养分含量的影响

采用盆栽试验研究土壤施硒对不同生长期苦荞N,P,K营养元素和土壤有效养分含量的影响。结果表明,除80 d的Se0.5处理外,施硒增加了苦荞茎的N含量,茎的P含量在60 d时除Se0.5处理外均显著降,40 d和80 d时均显著增加,施硒均降低苦荞全生育期茎的K含量。40 d时,叶的N,P含量降低,K含量在施硒≤1.5mg/kg范围升高0.46～0.8个百分点;60 d时,叶的N,P含量在低浓度硒时降低,高浓度硒时升高,K含量除Se0.5处理外均显著降低;80 d时叶的N,P含量除Se2.0处理外均降低,施硒各处理叶的K含量显著降低。花的N含量除Se0.5处理外均高于不施硒处理,P含量均不同程度降低,K含量显著提高。除Se0.5处理外,子粒的N,P,K均有所提高。施硒对速效氮的影响因硒水平和苦荞生育期各异,土壤有效磷、钾含量均随外源硒浓度的增加而增高。

滴灌施肥条件下107杨幼林N、P、K养分吸收量与施肥量研究

杨树以其生长迅速、产量大、分布广、适应性强、易繁殖、易更新等特性，成为我国主要的速生造林树种，常作为短轮伐期工业用材来经营；同时，它在解决我国用材短缺和生态问题等方面也起着不可忽视的作用。在营造速生丰产林的过程中，施肥是一项关键措施，多项研究结果表明，施肥对杨树树高、胸径的生长具有促进作用，不同施肥处理对杨树生长的影响不同。水和肥（主要是N肥）是影响杨树生长的重要因素，一直以来，

缙云山森林土壤速效N、P、K时空特征研究

研究了缙云山森林生态系统内 4个演替阶段群落的土壤速效 N、P、K的时空特征 ,结果表明 :1除灌草丛的速效 K外 ,速效 N、P、K含量在不同群落的土壤剖面上均具有明显的层次性 ,即腐殖质层 (A) >沉积层 (B) >母质层 (C)。 2 A、B两层的速效 N、K含量随群落演替方向升高 ,即灌草丛 <针叶林 <针阔混交林 <常绿阔叶林 ,速效 P含量则为 :针叶林 <针阔混交林 <灌草丛 <常绿阔叶林。 C层速效 N、P、K含量似乎与群落演替规律无必然联系。 3各群落内 A、B层土壤速效 N、P、K含量均表现出明显的季节动态 。

专用缓释肥料对黄瓜产量品质及N,P,K养分吸收利用的影响

采用土培试验研究了黄瓜专用缓释肥对黄瓜产量品质及N,P,K养分吸收利用、土壤酶活性的影响.结果表明:2种专用缓释肥(CSRF1和CSRF2)显著提高了黄瓜果实干物质量和总干物质量,CSRF1和CSRF2处理黄瓜果实干物质量较CCF,MSRF,OCF处理分别增加了23.3%~37.2%和16.5%~31.6%.2种专用缓释肥(CSRF2和CSRF1)提高了黄瓜果实的维生素C质量分数和氨基酸的质量分数,降低了硝酸盐质量分数.黄瓜根、茎、叶和果实的N,P,K养分质量分数均以CSRF2最高.黄瓜N,P,K积累量从大到小依次为CSRF2、CSRF1、MSRF、CCF、OCF,专用缓释肥处理的N,P,K积累量较MSRF,CCF和OCF处理分别提高了27.45%~46.55%,33.17%~56.20%和67.61%~102.13%.与普通复合肥相比,2种专用缓释肥处理的N,P,K相对养分利用效率分别增加了18.53%~24.20%,19.27%~20.02%和18.54%~35.69%.

常绿阔叶林藤本植物叶片N、P、K生态化学计量学特征

【目的】研究广西大明山国家级自然保护区常绿阔叶林中30种藤本植物叶片N、P、K生态化学计量学特征,为丰富群落生态化学计量学提供基础数据。【方法】在大明山天坪站保存完好的常绿阔叶林中设置20m×20m标准样地80块,共3.2ha,在每个标准样地左下角设置5m×5m的小样方,对藤本植物多样性进行调查,根据重要值,采集30种藤本植物叶片,进行叶片N、P、K含量测定。【结果】叶片N、P、K变化范围分别为3.52～54.71、0.21～3.36、0.48～4.23g/kg。各个化学计量因子之间存在一定的线性关系,草质藤本叶片N∶K含量均大于木质藤本,P含量差异不显著。【结论】大明山常绿阔叶林中多数藤本植物是受N或P或N∶P的影响,约30%的种类受到N元素限制,而受到P元素限制的植物相对少;草质藤本叶片比木质藤本有更强的N、K元素存储能力。

N·P·K用量对日光温室黄瓜产量及品质的影响

在日光温室内,研究不同的N、P、K用量对土壤栽培黄瓜产量及其品质的影响。研究结果表明:增加磷素用量和比例可以显著提高黄瓜的产量;适量氮钾肥配合增施磷肥可以增加果实中可溶性蛋白含量;适量氮磷肥配合增施钾肥可以改善果实品质,提高可溶性糖和维生素C含量,降低硝酸盐含量。

提高龙眼重回缩修剪树N,P,K营养水平和抽穗率的技术

对树体高大、交叉封行的龙眼树作重回缩修剪;剪后施用多效唑、乙烯利调节生长、控梢促花;测定叶片的N,P,K含量;调查翌年枝梢抽穗率。结果表明:①重回缩修剪对叶片N,P,K水平有明显影响,但对各次梢影响程度不同;②适当施用多效唑及乙烯利对提高重回缩修剪树叶片N,P,K营养水平及枝梢抽穗率有良好作用。

孕穗至灌浆期土壤渍水对冬小麦N、P、K素含量和积累量的影响

运用盆栽模拟土壤渍水逆境试验,研究孕穗期和灌浆期土壤渍水对不同小麦品种根系和地上部分N、P、K素吸收和运转影响。结果表明,土壤渍水逆境对不同小麦品种N、P、K素吸收的影响有异。孕穗期土壤渍水逆境显著影响根系对N、P素的吸收与运转,而对K素吸收与运转影响较小;灌浆期渍水逆境不仅影响根系对N、P、K素的吸收,同时也影响N、P素在地下与地上部器官的运转,但对K素在小麦体内的运转和分配影响较小。

N、P、K三要素对水稻产量的效应分析

利用数学模型研究了N、P、K对水稻产量的影响 ,分析了单因素效应、双因素效应和边际效应 ,得出三要素对产量的贡献率大小依次为 :N 1.2 0 81、P 1.0 5 2 7、K 0 .8499;N和P互作明显 ;当P处于高水平时 ,N的增产效果明显。说明高N、P配合适当的K是提高水稻产量的重要途径之一。