In this study,the quasi-static ebulliometric method was used to measure both of the vapor pressures of methyl N-phenyl carbamate(MPC),and the isobaric vapor–liquid equilibrium(VLE) data of the aniline and MPC binary ...In this study,the quasi-static ebulliometric method was used to measure both of the vapor pressures of methyl N-phenyl carbamate(MPC),and the isobaric vapor–liquid equilibrium(VLE) data of the aniline and MPC binary system.The measured vapor pressure data of MPC,at different temperature ranging from 369.60 to 389.54 K,fitted well with the Antoine equation.The VLE data for the aniline and MPC system at(2.00,4.00,6.00,7.00 and 8.00) k Pa were correlated by both of nonrandom two-liquid(NRTL) and Wilson models.The parameters of the two models were obtained by regressing the experimental data,with the absolute temperature deviations of 0.54 K and 0.53 K,respectively.The relative volatility of the binary system calculated was all far more than 1,which gives the conclusion that the high purity MPC can be separated from aniline and MPC binary system by rectification or distillation technology.展开更多
Objective To evaluate and compare the ability to detect tumor by bis (N-ethoxy-N-ethyl dithiocarbamato) nitrido99mTc(V) [99mTcN(NOEt)2] and99mTc hexakis-2- methoxyisobutyl isonitrile [99mTc-MIBI]. Methods 99mTcN(NOE...Objective To evaluate and compare the ability to detect tumor by bis (N-ethoxy-N-ethyl dithiocarbamato) nitrido99mTc(V) [99mTcN(NOEt)2] and99mTc hexakis-2- methoxyisobutyl isonitrile [99mTc-MIBI]. Methods 99mTcN(NOEt)2 was prepared and quality control was performed using ascending thin-layer chromatography. Four mice bearing Ehrich ascites tumor cells underwent whole body planar imaging at 30 min, 2 h and 4 h after injection of99mTc-MIBI or99mTcN(NOEt)2. ROIs were drawn around the tumor, head, chest, and contralateral limbs in whole body planar images, and ratios of radioactivity in tumor in head (T/H), chest (T/C), and contralateral limbs (T/L) were calculated. The mice of99mTcN (NOEt)2 group were killed, then blood was collected, and the tumor and organs were excised, weighed and the radioactivity was measured. Results 99mTcN(NOEt)2 was stable after 4h at the room temperature.99mTcN(NOEt)2 was delivered to the tumor selectively and efficiently.99mTcN(NOEt)2 was found to provide excellent tumor-to-nontumor contrast for all the tissue except the abdomen. The T/L ratios increased to their maximums (4.87) at 2 h after injection. There was significant difference between the99mTcN(NOEt)2 imaging group and99mTc-MIBI imaging group. In vitro the radioactivity ratios per unit weight of tumor to blood, muscle, skeleton, lung, heart, and spleen were much higher than those of tumor to liver, instestine. Conclusion In mice bearing Ehrich ascites tumor,99mTcN(NOEt)2 exhibits a set of features essential for a good tracer for tumor imaging, including a rapid washout from blood, high uptake rate in tumor tissue, prolonged retention and high tumor-to-nontumor uptake ratio. The imaging quality of99mTcN(NOEt)2 was superior to that of99mTc-MIBI. These features indicate that99mTcN(NOEt)2 may be a better tracer to detect tumor than99mTc-MIBI. Key words tumor - radionuclide imaging - NOEt - MIBI展开更多
As a novel collector, O-isopropyl-N,N-diethyl thionocarbamate(IPDTC) was designed and synthesized for copper-sulfur flotation separation. Density functional theory calculations were performed to investigate the electr...As a novel collector, O-isopropyl-N,N-diethyl thionocarbamate(IPDTC) was designed and synthesized for copper-sulfur flotation separation. Density functional theory calculations were performed to investigate the electronic structures of IPDTC. The results showed that IPDTC had higher energy of the highest occupied molecular orbital but lower electronegativity than O-isopropyl-N-ethyl thionocarbamate(Z-200). It was predicted that IPDTC had strong collection ability according to the reaction energy criteria. Flotation results demonstrated that the collecting ability of IPDTC to chalcopyrite and pyrite was stronger than that of Z-200. Then, the flotation mechanism was analyzed by measurements of surface tension, adsorption capacity, XPS, FTIR and zeta potential. These results indicated that IPDTC could reduce the solution surface tension. The adsorption capacity of IPDTC on chalcopyrite was higher than that on pyrite, consistent with the results of the flotation tests. FTIR, zeta potential and XPS results also demonstrated that IPDTC was strongly absorbed on the chalcopyrite surface by formation of Cu—S—C bonds, but showed a weak affinity on the pyrite surface.展开更多
Objective： To explore the molecular biological mechanism of acupuncture in intervening visual deprivation. Methods： Forty-eight 2-week old Wistar rats were randomly divided into a normal group, a model group, and 6 ...Objective： To explore the molecular biological mechanism of acupuncture in intervening visual deprivation. Methods： Forty-eight 2-week old Wistar rats were randomly divided into a normal group, a model group, and 6 acupuncture groups （group C1： acupuncture at the unaffected side in early stage; group C2： acupuncture at the affected side in early stage; group DI： acupuncture at the unaffected side in mid-stage; group D2： acupuncture at the affected side in mid-stage; group El： acupuncture at the unaffected side in late stage; group E2： acupuncture at the affected side in late stage） by the random number table, 6 rats in each group. Rats in the normal group didn＇t receive any interventions. The rat model of deprivation amblyopia was established by unilateral eyelid suture in the model group and each acupuncture group After successful modeling, rats in model group didn＇t receive any treatments; rats in the acupuncture groups received acupuncture intervention which began respectively on the 3rd, 12th and 21st day after modeling. Pattern visual evoked potential （P-VEP） and N-methy D-aspartatreceptor-1 （NMDAR1） mRNA expression in visual cortex area 17 were detected at the end of acupuncture intervention in each group. Results： After the intervention, the P-VEP waveform was significantly changed, with a significantly delayed Pzoo value （P〈0.01） and significantly decreased amplitude of N45-Ploo in the model group versus the normal group （P〈O.01）; the P-VEP waveform was significantly improved, with obviously earlier P10o （P〈0.01） and increased amplitude of N4s-Ploo （P〈O.05） in each acupuncture group versus the model group. The improvement effect of acupuncture on the P-VEP waveform in group C1 and C2 was more significant than that in group D1, D2, E1 and E2. The expression of NMDAR1 mRNA of the rat visual cortex area 17 in the model group was significantly lower than that in the normal group （P〈0.01）; and the expression of NMDAR1 mRNA in the visual cortex area 17 of each acupuncture group was significantly higher than that in the model group （P〈0.05）; the effect of acupuncture on NMDAR1 mRNA expression in group C1 and C2 was more significant than that in group D1, D2, E1 and E2; and the effect of acupuncture on NMDAR1 mRNA expression was better in group C2 than in group C1 （P〈O.05）; there was no significant difference in the expression of NMDAR1 mRNA between group D1 and D2, neither between E1 and E2 （P〉0.05）. Conclusion： P-VEP waveform is abnormal and NMDAR1 mRNA expression in visual cortex area 17 is decreased in rats with monocularly-deprived amblyopia. Acupuncture in the sensitive period can significantly regulate the abnormal P-VEP waveform and the down-regulate the NMDAR1 mRNA expression of the visual cortex of rats with monocularly-deprived amblyopia. Early treatment in the sensitive period should be the key to obtaining the curative effect.展开更多
基金Supported by the National Key Technology R&D Program(2013BAC11B03)the National Natural Science Foundation of China(21206180,21406245,21476244)
文摘In this study,the quasi-static ebulliometric method was used to measure both of the vapor pressures of methyl N-phenyl carbamate(MPC),and the isobaric vapor–liquid equilibrium(VLE) data of the aniline and MPC binary system.The measured vapor pressure data of MPC,at different temperature ranging from 369.60 to 389.54 K,fitted well with the Antoine equation.The VLE data for the aniline and MPC system at(2.00,4.00,6.00,7.00 and 8.00) k Pa were correlated by both of nonrandom two-liquid(NRTL) and Wilson models.The parameters of the two models were obtained by regressing the experimental data,with the absolute temperature deviations of 0.54 K and 0.53 K,respectively.The relative volatility of the binary system calculated was all far more than 1,which gives the conclusion that the high purity MPC can be separated from aniline and MPC binary system by rectification or distillation technology.
文摘Objective To evaluate and compare the ability to detect tumor by bis (N-ethoxy-N-ethyl dithiocarbamato) nitrido99mTc(V) [99mTcN(NOEt)2] and99mTc hexakis-2- methoxyisobutyl isonitrile [99mTc-MIBI]. Methods 99mTcN(NOEt)2 was prepared and quality control was performed using ascending thin-layer chromatography. Four mice bearing Ehrich ascites tumor cells underwent whole body planar imaging at 30 min, 2 h and 4 h after injection of99mTc-MIBI or99mTcN(NOEt)2. ROIs were drawn around the tumor, head, chest, and contralateral limbs in whole body planar images, and ratios of radioactivity in tumor in head (T/H), chest (T/C), and contralateral limbs (T/L) were calculated. The mice of99mTcN (NOEt)2 group were killed, then blood was collected, and the tumor and organs were excised, weighed and the radioactivity was measured. Results 99mTcN(NOEt)2 was stable after 4h at the room temperature.99mTcN(NOEt)2 was delivered to the tumor selectively and efficiently.99mTcN(NOEt)2 was found to provide excellent tumor-to-nontumor contrast for all the tissue except the abdomen. The T/L ratios increased to their maximums (4.87) at 2 h after injection. There was significant difference between the99mTcN(NOEt)2 imaging group and99mTc-MIBI imaging group. In vitro the radioactivity ratios per unit weight of tumor to blood, muscle, skeleton, lung, heart, and spleen were much higher than those of tumor to liver, instestine. Conclusion In mice bearing Ehrich ascites tumor,99mTcN(NOEt)2 exhibits a set of features essential for a good tracer for tumor imaging, including a rapid washout from blood, high uptake rate in tumor tissue, prolonged retention and high tumor-to-nontumor uptake ratio. The imaging quality of99mTcN(NOEt)2 was superior to that of99mTc-MIBI. These features indicate that99mTcN(NOEt)2 may be a better tracer to detect tumor than99mTc-MIBI. Key words tumor - radionuclide imaging - NOEt - MIBI
基金financial supports from the Open Foundation of State Key Laboratory of Mineral Processing,China (Nos.BGRIMM-KJSKL-2019-06,BGRIMMKJSKL-2022-13)the Open Fund of State Key Laboratory of Comprehensive Utilization of Low-Grade,China (No.ZJKY2017(B)KFJJ003)。
文摘As a novel collector, O-isopropyl-N,N-diethyl thionocarbamate(IPDTC) was designed and synthesized for copper-sulfur flotation separation. Density functional theory calculations were performed to investigate the electronic structures of IPDTC. The results showed that IPDTC had higher energy of the highest occupied molecular orbital but lower electronegativity than O-isopropyl-N-ethyl thionocarbamate(Z-200). It was predicted that IPDTC had strong collection ability according to the reaction energy criteria. Flotation results demonstrated that the collecting ability of IPDTC to chalcopyrite and pyrite was stronger than that of Z-200. Then, the flotation mechanism was analyzed by measurements of surface tension, adsorption capacity, XPS, FTIR and zeta potential. These results indicated that IPDTC could reduce the solution surface tension. The adsorption capacity of IPDTC on chalcopyrite was higher than that on pyrite, consistent with the results of the flotation tests. FTIR, zeta potential and XPS results also demonstrated that IPDTC was strongly absorbed on the chalcopyrite surface by formation of Cu—S—C bonds, but showed a weak affinity on the pyrite surface.
基金supported by National Natural Science Foundation of China,No.81260560~~
文摘Objective： To explore the molecular biological mechanism of acupuncture in intervening visual deprivation. Methods： Forty-eight 2-week old Wistar rats were randomly divided into a normal group, a model group, and 6 acupuncture groups （group C1： acupuncture at the unaffected side in early stage; group C2： acupuncture at the affected side in early stage; group DI： acupuncture at the unaffected side in mid-stage; group D2： acupuncture at the affected side in mid-stage; group El： acupuncture at the unaffected side in late stage; group E2： acupuncture at the affected side in late stage） by the random number table, 6 rats in each group. Rats in the normal group didn＇t receive any interventions. The rat model of deprivation amblyopia was established by unilateral eyelid suture in the model group and each acupuncture group After successful modeling, rats in model group didn＇t receive any treatments; rats in the acupuncture groups received acupuncture intervention which began respectively on the 3rd, 12th and 21st day after modeling. Pattern visual evoked potential （P-VEP） and N-methy D-aspartatreceptor-1 （NMDAR1） mRNA expression in visual cortex area 17 were detected at the end of acupuncture intervention in each group. Results： After the intervention, the P-VEP waveform was significantly changed, with a significantly delayed Pzoo value （P〈0.01） and significantly decreased amplitude of N45-Ploo in the model group versus the normal group （P〈O.01）; the P-VEP waveform was significantly improved, with obviously earlier P10o （P〈0.01） and increased amplitude of N4s-Ploo （P〈O.05） in each acupuncture group versus the model group. The improvement effect of acupuncture on the P-VEP waveform in group C1 and C2 was more significant than that in group D1, D2, E1 and E2. The expression of NMDAR1 mRNA of the rat visual cortex area 17 in the model group was significantly lower than that in the normal group （P〈0.01）; and the expression of NMDAR1 mRNA in the visual cortex area 17 of each acupuncture group was significantly higher than that in the model group （P〈0.05）; the effect of acupuncture on NMDAR1 mRNA expression in group C1 and C2 was more significant than that in group D1, D2, E1 and E2; and the effect of acupuncture on NMDAR1 mRNA expression was better in group C2 than in group C1 （P〈O.05）; there was no significant difference in the expression of NMDAR1 mRNA between group D1 and D2, neither between E1 and E2 （P〉0.05）. Conclusion： P-VEP waveform is abnormal and NMDAR1 mRNA expression in visual cortex area 17 is decreased in rats with monocularly-deprived amblyopia. Acupuncture in the sensitive period can significantly regulate the abnormal P-VEP waveform and the down-regulate the NMDAR1 mRNA expression of the visual cortex of rats with monocularly-deprived amblyopia. Early treatment in the sensitive period should be the key to obtaining the curative effect.
