To investigate the role of N-cadherin and fibronectin during chondrogenesis. Methods - lmmunohistochemical method and autibody induced changes of aggregation of cells were used to assay the expressions of N-cadherin ...To investigate the role of N-cadherin and fibronectin during chondrogenesis. Methods - lmmunohistochemical method and autibody induced changes of aggregation of cells were used to assay the expressions of N-cadherin and fibronectin during cell differentiation. Results： The N-cadherin was present in the area of the cell nodular area in the 24 hours group after adding chondrogenic revulsant, then there was a down-regulating trend. Fibronectin was expressed in 48 and 72 hours groups after adding chondrogenic revulsant, and showed to be negative afterward. The antibody against fibronectin or Ncadherin could inhibit the formation of cellular nodule markedly. Conclusions： Cell adhesion factors play an important role during cell differentiation. TGF-β1 stimulates chondrogenesis via transition from an initial N-cadherin-contributing stage to a succedent fibronectin-contributing stage during the process of chondrogenesis in MSCs. Further study is needed to evaluate whether or not it can promote chondrogenesis by transfecting cDNA of CAMs to MSCs.展开更多
文摘To investigate the role of N-cadherin and fibronectin during chondrogenesis. Methods - lmmunohistochemical method and autibody induced changes of aggregation of cells were used to assay the expressions of N-cadherin and fibronectin during cell differentiation. Results： The N-cadherin was present in the area of the cell nodular area in the 24 hours group after adding chondrogenic revulsant, then there was a down-regulating trend. Fibronectin was expressed in 48 and 72 hours groups after adding chondrogenic revulsant, and showed to be negative afterward. The antibody against fibronectin or Ncadherin could inhibit the formation of cellular nodule markedly. Conclusions： Cell adhesion factors play an important role during cell differentiation. TGF-β1 stimulates chondrogenesis via transition from an initial N-cadherin-contributing stage to a succedent fibronectin-contributing stage during the process of chondrogenesis in MSCs. Further study is needed to evaluate whether or not it can promote chondrogenesis by transfecting cDNA of CAMs to MSCs.
