Most rhizobial inoculants that stimulate legume yield are applied with carriers that enhance root contact. The physicochemical properties of biochar are suitable for microbial growth, and it could be an alternative to...Most rhizobial inoculants that stimulate legume yield are applied with carriers that enhance root contact. The physicochemical properties of biochar are suitable for microbial growth, and it could be an alternative to peat, which comes from decreasing reserves but is the commonest solid inoculant carrier. The aim of the current research was to evaluate biochars as carriers of bradyrhizobia in solid inoculant and as coatings for seeds. Biochars and peat were inoculated with Bradyrhizobium japonicum strain 532C and storage time was assessed. A seed coating system was developed using biochar, bacteria liquid culture, water, and guar gum. The viability of bacteria in the coating and in solid biochar was evaluated at 4°C and 21°C. Two biochars were selected for a germination assay. Finally, greenhouse experimentation investigated the effect of biochar inoculant and seed coating on soybean growth and nutrient uptake. The storage time experiment showed that not all biochars equally sustain bacteria survival over time. The germination assay demonstrated that biochar seed coating had no effect on soybean germination. Greenhouse experimentation indicated that the effect of Pyrovac biochar on soybean growth characteristics and nutrient uptake depended on the fertilizer. The main finding was that biochar solid inoculant positively affected plant growth metrics, root characteristics, and the chemical composition of plants supplied with N-free nutrient solution.展开更多
The information related with results of a project on germplasm characterization of three Lotus species, important as forage plants, curried out in five environments of the central-southern zone of Chile, was reviewed....The information related with results of a project on germplasm characterization of three Lotus species, important as forage plants, curried out in five environments of the central-southern zone of Chile, was reviewed. An evaluation was made of 12 L. corniculatus cultivars introduced from different parts of the world, as well as germplasm naturalized in Chile, being 11 accessions of L. tenuis and 21 accessions ofL. uliginosus collected from 33°S to 38°S and 38°S to 45°S, respectively. Phenological, morphological, and agronomical characters were evaluated. Nitrogen fixation in field conditions and concentrations of condensed tannins in herbage were also included. It is concluded that there are cultivars of high value in L. corniculatus introduced germplasm which can be recommended for different environments of the central zone of Chile, as well as for local species breeding programmes. Information obtained from L. tenuis and L. uliginosus accessions shows genetic variability within both species. L. tenuis germplasm is adapted to clay, medium-textured, or sandy soils with water restrictions and phosphorous deficiency. Therefore, characterized accessions could be used to breed cultivars for low input production systems in constrained environments. L. uliginosus accessions include genotypes collected in sites with acid soils and variable tannin content in plant tissues that may be promissory genetic materials for breeding programmes with the objective of producing cultivars for animal production systems for the wet and acid soils in the southern zone of the country.展开更多
The investigation was conducted to know the effect of 2, 4-Dichlorophenoxy acetic acid and inoculation on Para-nodule formation and growth parameters in wheat. Formation of nodular outgrowth on the roots of wheat seed...The investigation was conducted to know the effect of 2, 4-Dichlorophenoxy acetic acid and inoculation on Para-nodule formation and growth parameters in wheat. Formation of nodular outgrowth on the roots of wheat seedlings treated with 2, 4-D commenced in 8-10 days. The maximum Para-nodule formation was found in 1 ppm of 2, 4-D when incubated with bradyrhizobium P-132. Inoculation of the same has helped to increase the number of Para-nodule, but not essential for Para-nodulation. Histological study shows that, these induced Para-nodules originated from the pericycle and these are appeared to be modified lateral roots and Para-nodule structure formations however, it enhanced by bradyrhizobial inoculation.展开更多
文摘Most rhizobial inoculants that stimulate legume yield are applied with carriers that enhance root contact. The physicochemical properties of biochar are suitable for microbial growth, and it could be an alternative to peat, which comes from decreasing reserves but is the commonest solid inoculant carrier. The aim of the current research was to evaluate biochars as carriers of bradyrhizobia in solid inoculant and as coatings for seeds. Biochars and peat were inoculated with Bradyrhizobium japonicum strain 532C and storage time was assessed. A seed coating system was developed using biochar, bacteria liquid culture, water, and guar gum. The viability of bacteria in the coating and in solid biochar was evaluated at 4°C and 21°C. Two biochars were selected for a germination assay. Finally, greenhouse experimentation investigated the effect of biochar inoculant and seed coating on soybean growth and nutrient uptake. The storage time experiment showed that not all biochars equally sustain bacteria survival over time. The germination assay demonstrated that biochar seed coating had no effect on soybean germination. Greenhouse experimentation indicated that the effect of Pyrovac biochar on soybean growth characteristics and nutrient uptake depended on the fertilizer. The main finding was that biochar solid inoculant positively affected plant growth metrics, root characteristics, and the chemical composition of plants supplied with N-free nutrient solution.
文摘The information related with results of a project on germplasm characterization of three Lotus species, important as forage plants, curried out in five environments of the central-southern zone of Chile, was reviewed. An evaluation was made of 12 L. corniculatus cultivars introduced from different parts of the world, as well as germplasm naturalized in Chile, being 11 accessions of L. tenuis and 21 accessions ofL. uliginosus collected from 33°S to 38°S and 38°S to 45°S, respectively. Phenological, morphological, and agronomical characters were evaluated. Nitrogen fixation in field conditions and concentrations of condensed tannins in herbage were also included. It is concluded that there are cultivars of high value in L. corniculatus introduced germplasm which can be recommended for different environments of the central zone of Chile, as well as for local species breeding programmes. Information obtained from L. tenuis and L. uliginosus accessions shows genetic variability within both species. L. tenuis germplasm is adapted to clay, medium-textured, or sandy soils with water restrictions and phosphorous deficiency. Therefore, characterized accessions could be used to breed cultivars for low input production systems in constrained environments. L. uliginosus accessions include genotypes collected in sites with acid soils and variable tannin content in plant tissues that may be promissory genetic materials for breeding programmes with the objective of producing cultivars for animal production systems for the wet and acid soils in the southern zone of the country.
文摘The investigation was conducted to know the effect of 2, 4-Dichlorophenoxy acetic acid and inoculation on Para-nodule formation and growth parameters in wheat. Formation of nodular outgrowth on the roots of wheat seedlings treated with 2, 4-D commenced in 8-10 days. The maximum Para-nodule formation was found in 1 ppm of 2, 4-D when incubated with bradyrhizobium P-132. Inoculation of the same has helped to increase the number of Para-nodule, but not essential for Para-nodulation. Histological study shows that, these induced Para-nodules originated from the pericycle and these are appeared to be modified lateral roots and Para-nodule structure formations however, it enhanced by bradyrhizobial inoculation.
