Up to now, there have been many indistinctive points in the image interpretation of scanning tunnelhng microscopy (STM) system, and the problems have attracted almost all STM scientists’ attention. In 1992, Kenkre pr...Up to now, there have been many indistinctive points in the image interpretation of scanning tunnelhng microscopy (STM) system, and the problems have attracted almost all STM scientists’ attention. In 1992, Kenkre proposed the new programme which describes the behaviour of the electrons moving in the STM tunnelling by the method of exciton dynamics, and the programme not only breaks through the restrictions of Tersoff-Hamann theory, but also can be applied conveniently to discussing the effects of the tip structure, adsorbate structure, substrate structure, temperature, tunneling voltage, and the degree of coherence of elec-展开更多
目的构建带有MERS-Co V N(MERS冠状病毒核衣壳蛋白)基因片段的p ET-22b+原核表达载体,并表达和纯化核衣壳蛋白(NP)。方法通过PCR技术扩增NP蛋白基因片段,插入到原核表达载体p ET-22b+上。使用核酸电泳、测序以及小量诱导表达确认所构建...目的构建带有MERS-Co V N(MERS冠状病毒核衣壳蛋白)基因片段的p ET-22b+原核表达载体,并表达和纯化核衣壳蛋白(NP)。方法通过PCR技术扩增NP蛋白基因片段,插入到原核表达载体p ET-22b+上。使用核酸电泳、测序以及小量诱导表达确认所构建克隆的正确性,然后将重组质粒转化进大肠杆菌BL21a(DE3)中,并在大肠杆菌BL21a(DE3)中大规模诱导表达NP蛋白,使用AKTA纯化系统经强阳离子交换层析纯化出NP蛋白。结果测序和小量诱导结果表明,扩增的NP蛋白片段序列正确,所构建的p ET-22b+-NP克隆可在大肠杆菌BL21a(DE3)的包涵体和细胞质中表达;通过阳离子交换层析和浓缩后,蛋白的纯度和浓度都得到明显提高。结论纯化出高纯度、高浓度的NP蛋白,为NP蛋白功能性研究提供重要基础。展开更多
文摘Up to now, there have been many indistinctive points in the image interpretation of scanning tunnelhng microscopy (STM) system, and the problems have attracted almost all STM scientists’ attention. In 1992, Kenkre proposed the new programme which describes the behaviour of the electrons moving in the STM tunnelling by the method of exciton dynamics, and the programme not only breaks through the restrictions of Tersoff-Hamann theory, but also can be applied conveniently to discussing the effects of the tip structure, adsorbate structure, substrate structure, temperature, tunneling voltage, and the degree of coherence of elec-
