A reduced computational load protein coding predictor using equivalent amino acid sequence of DNA string with period-3 based time and frequency domain analysis

Development of efficient gene prediction algorithms is one of the fundamental efforts in gene prediction study in the area of genomics. In genomic signal processing the basic step of the identification of protein coding regions in DNA sequences is based on the period-3 property exhibited by nucleotides in exons. Several approaches based on signal processing tools and numerical representations have been applied to solve this problem, trying to achieve more accurate predictions. This paper presents a new indicator sequence based on amino acid sequence, called as aminoacid indicator sequence, derived from DNA string that uses the existing signal processing based time-domain and frequency domain methods to predict these regions within the billions long DNA sequence of eukaryotic cells which reduces the computational load by one-third. It is known that each triplet of bases, called as codon, instructs the cell machinery to synthesize an amino acid. The codon sequence therefore uniquely identifies an amino acid sequence which defines a protein. Thus the protein coding region is attributed by the codons in amino acid sequence. This property is used for detection of period-3 regions using amino acid sequence. Physico-chemical properties of amino acids are used for numerical representation. Various accuracy measures such as exonic peaks, discriminating factor, sensitivity, specificity, miss rate, wrong rate and approximate correlation are used to demonstrate the efficacy of the proposed predictor. The proposed method is validated on various organisms using the standard data-set HMR195, Burset and Guigo and KEGG. The simulation result shows that the proposed method is an effective approach for protein coding prediction.

Pathogenicity and amino acid sequences of hemagglutinin cleavage site and neuraminidase stalk of differently passaged H9N2-avian influenza virus in broilers

Low pathogenic Avian Influenza (AI) virus has the ability to evolve to high pathogenic viruses resulting in significant economic losses in the poultry sector. This study aims at assessing the impact of H9N2 viral passaging in broilers and its relatedness to pathogenicity and amino acid (a.a) sequences of the hemagglutinin (HA) cleavage site and neuraminidase (NA) stalk. The original H9N2 AI virus (P0) was used to challenge ten-21 days old broilers. Individual recovery of H9N2 virus from homogenates of trachea, lungs and airsacs was attempted in 9 days old chicken embryos, as a conclusion of the first passage (P1). Tracheal isolates of H9N2 were passaged for a second (P2) and a third (P3) time in broilers, followed by a similar embryonic recovery procedure. The a.a. sequence of a part of HA1 cleavage site and Neuraminidase stalk were compared among the differently passaged viruses;an assessement of the relatedness of the determined a.a. sequences to the pathogenicity in broilers, based on frequency of mortality, morbidity signs, gross and microscopic lesions at 3 days post challenge with the P1, P2, and P3-H9N2, is concluded. An increase in certain morbidity signs and specific lesions was observed in P2- and P3-H9N2 challenged broilers compared to birds challenged with P1-H9N2. A conserved R-S-S-R amino acid sequence at the HA1 cleavage site was observed in the differently passaged H9N2, associated with a variability in the NA stalk-a.a sequences. The passaging of the low pathogenic H9N2 virus in broilers leads to a trend of increase in pathogenicity, manifested in higher frequency of morbidity signs, and of specific gross and microscopic lesions of the examined organs. This passaging was associated with a conserved a.a. sequence of the hemaglutinin cleavage site and a variability in the sequence of the neuraminidase stalk. A detailed study of the potential of the detected variability in the neuraminidase stalk of H9N2 in induction of a higher pathogenicity in broilers will be the subject of future investigations.

The structural analysis of protein sequences based on the quasi-amino acids code

Proteomics is the study of proteins and their interactions in a cell. With the successful completion of the Human Cenome Project, it comes the postgenome era when the proteomics technology is emerging. This paper studies protein molecule from the algebraic point of view. The algebraic system （∑, ＋, ＊） is introduced, where ∑ is the set of 64 codons. According to the characteristics of （∑, ＋, ＊）, a novel quasi-amino acids code classification method is introduced and the corresponding algebraic operation table over the set ZU of the 16 kinds of quasi-amino acids is established. The internal relation is revealed about quasi-amino acids. The results show that there exist some very close correlations between the properties of the quasi-amino acids and the codon. All these correlation relationships may play an important part in establishing the logic relationship between codons and the quasi-amino acids during the course of life origination. According to Ma F et al （2003 J. Anhui Agricultural University 30 439）, the corresponding relation and the excellent properties about amino acids code are very difficult to observe. The present paper shows that （ZU, ＋,×） is a field. Furthermore, the operational results display that the eodon tga has different property from other stop codons. In fact, in the mitochondrion from human and ox genomic codon, tga is just tryptophane, is not the stop codon like in other genetic code, it is the case of the Chen W C et al （2002 Acta Biophysiea Siniea 18（1） 87）. The present theory avoids some inexplicable events of the 20 kinds of amino acids code, in other words it solves the problem of ＇the 64 codon assignments of mRNA to amino acids is probably completely wrong＇ proposed by Yang （2006 Progress in Modern Biomedicine 6 3）.

Proteolipid protein 1 gene sequencing of hereditary spastic paraplegia

PCR amplification and sequencing of whole blood DNA from an individual with hereditary spastic paraplegia, as well as family members, revealed a fragment of proteolipid protein 1 （PLP1） gene exon 1, which excluded the possibility of isomer 1 expression for this family. The fragment sequence of exon 3 and exon 5 was consistent with the proteolipid protein 1 sequence at NCBI. In the proband samples, a PLP1 point mutation in exon 4 was detected at the basic group of position 844, T→C, phenylalanine→leucine. In proband samples from a male cousin, the basic group at position 844 was C, but gene sequencing signals revealed mixed signals of T and C, indicating possible mutation at this locus. Results demonstrated that changes in PLP1 exon 4 amino acids were associated with onset of hereditary spastic paraplegia.

Sequencing of a mutated TNFα by using pinpoint fusion protein expression system

The amino acid sequencing on mutation region of tumor necmeis factor a derivative 3a(rh-TNFaD3a).Methods: PCR was used to produce the TNF half molecule which contains the mutation "don in itS 5’ end.Then, a fusion protein expression system was used to express the TNF half molecule. The fusion protein waspubbed by sanity chromatography and enzymatically cleaved by Xa factor protease to remove fusion vectorpeptide. Finally, the N-terminal amino acid sequencing was performed. Results and Conclusion: The site-specific artificial mutation of TNFαD3a was realized as expected: position 80 Ile→Ser, position 90 Lys→Hisand position 92 Asn→Val.

澳洲坚果抗菌肽分离纯化及其肽段鉴定与分析

以液压压榨澳洲坚果粕为原料,采用碱性蛋白酶水解制备澳洲坚果多肽(macadamia nut peptide⁃0,MNP⁃0),以对金黄色葡萄球菌与白色念珠菌的抑菌活性为跟踪指标,通过超滤、DA201⁃C型大孔吸附树脂、交联葡聚糖凝胶Sephadex G⁃25对其进行逐级分离纯化,获得抑菌活性较强的抗菌肽,并运用液相色谱串联质谱(liquid chromatography tandem mass spectrometry,LC⁃MS/MS)技术对其进行肽段组成与氨基酸序列分析。结果表明:超滤分离的不同分子量澳洲坚果多肽在所有多肽中所占质量分数不同,抑菌活性也有所差异。其中,澳洲坚果多肽组分MNP⁃4占比最高,为29.55%;抑菌活性也最强,对金黄色葡萄球菌与白色念珠菌的抑菌圈直径分别为10.01 mm与9.41 mm。大孔吸附树脂纯化多肽组分MNP⁃4的技术条件为75%乙醇溶液为解吸剂,静态吸附3 h,动态洗脱体积60 mL。经大孔吸附树脂与交联葡聚糖凝胶Sephadex G⁃25分离纯化,获得3个澳洲坚果纯化多肽(macadamia nut purified peptide,MPP)组分,其中组分MPP⁃2的抑菌活性最强,对金黄色葡萄球菌与白色念珠菌的抑菌圈直径分别为18.67 mm与16.83 mm,优于多肽MNP⁃0与超滤分离多肽组分。分离纯化的澳洲坚果抗菌活性多肽MPP⁃2含有DDLTDPAPA、VLL、WDY、VPV、WDL、LLW、LWL、FDW 8个肽段,经预测分析,属于抗菌肽的肽段为WDL与FDW,其概率得分a(1.00≥a≥0.50)均为1.00,疏水率均为66.67%,均带有1个负电荷,等电点分别为0.69与0.67,并具有较好的溶解性。研究结果表明澳洲坚果可通过酶法制备具有抗菌活性的肽类成分。

氨基酸配方替代喂养对重度牛奶蛋白过敏婴儿肠道菌群特征的影响

目的探讨氨基酸配方(AAF)替代喂养对重度牛奶蛋白过敏(CMPA)婴儿肠道菌群特征的影响。方法纳入23例确诊为重度CMPA且人工喂养的≤3月龄婴儿,采用同一品牌AAF进行喂养干预。干预前及干预3个月后,收集所有婴儿的粪便样本,通过16S rRNA高通量测序及生物信息学分析评估肠道菌群的特征变化。结果干预前后,重度CMPA婴儿肠道菌群Chao1指数、Shannon指数、observed species指数、Simpson指数差异具有统计学意义(P<0.05),Beta多样性具有差异,且组间差异大于组内差异。门水平的相对丰度分析及LEfSe分析结果均显示,AAF干预后重度CMPA婴儿肠道中厚壁菌门、拟杆菌门显著富集。属水平的相对丰度分析结果显示,AAF干预后重度CMPA婴儿肠道中拟杆菌属、粪杆菌属、双歧杆菌属的相对丰度较干预前明显升高,弓形菌属、克雷伯菌属、梭菌属的相对丰度较干预前降低,LEfSe分析结果显示拟杆菌属、粪杆菌属、弓形菌属、克雷伯菌属为在丰度上有显著差异的标志物种。在种水平上相对丰度排名前10的菌群中,产气荚膜梭菌、产酸拟杆菌、丁酸梭菌、格氏乳杆菌、罗伊氏乳杆菌的相对丰度显著升高。结论AAF替代喂养可使得重度CMPA患儿肠道菌群多样性增加,拟杆菌、厚壁菌等的丰度增加。

Isolation and Characterization of SARS-CoV-2 in Kenya

The discovery of Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) in Wuhan, Hubei province, China, in December 2019 raised global health warnings. Quickly, in 2020, the virus crossed borders and infected individuals across the world, evolving into the COVID-19 pandemic. Notably, early signs of the virus’s existence were observed in various countries before the initial outbreak in Wuhan. As of 12th of April, the respiratory disease had infected over 762 million people worldwide, with over 6.8 million deaths recorded. This has led scientists to focus their efforts on understanding the virus to develop effective means to diagnose, treat, prevent, and control this pandemic. One of the areas of focus is the isolation of this virus, which plays a crucial role in understanding the viral dynamics in the laboratory. In this study, we report the isolation and detection of locally circulating SARS-CoV-2 in Kenya. The isolates were cultured on Vero Cercopithecus cell line (CCL-81) cells, RNA extraction was conducted from the supernatants, and reverse transcriptase-polymerase chain reaction (RT-PCR). Genome sequencing was done to profile the strains phylogenetically and identify novel and previously reported mutations. Vero CCL-81 cells were able to support the growth of SARS-CoV-2 in vitro, and mutations were detected from the two isolates sequenced (001 and 002). Genome sequencing revealed the circulation of two isolates that share a close relationship with the Benin isolate with the D614G common mutation identified along the S protein. These virus isolates will be expanded and made available to the Kenya Ministry of Health and other research institutions to advance SARS-CoV-2 research in Kenya and the region.

菜籽饼超微粉酶解制备抗氧化活性肽

为探究菜籽饼超微粉直接酶解制备抗氧化肽技术,该研究以菜籽饼超微粉为原料,利用碱性蛋白酶、风味蛋白酶对其进行酶解,通过测定2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)(ABTS)自由基清除能力、1,1-二苯基-2-苦基肼自由基清除能力和总抗氧化能力表征酶解产物体抗氧化活性,使用膜分离、Sephadex G-15凝胶层析对酶解产物进行分离纯化,利用高效液相色谱-串联质谱联用技术分析鉴定抗氧化活性组分的氨基酸序列。结果表明,风味蛋白酶酶解产物的肽含量较高,并以分子量小于1 kDa肽段为主,碱性蛋白酶解产物以5~10 kDa肽段为主,但同等浓度下(1 mg/mL)的碱性蛋白酶酶解产物抗氧化活性较高;膜分离后,同种酶解产物中低分子量(<1 kDa)组分具有更好的ABTS自由基清除能力和总抗氧化活性;通过凝胶层析分离纯化得到的高抗氧化活性组分J3经氨基酸序列鉴定,结合PeptideRanker生物活性预测筛选得到5个肽段,肽段的N-端均为苯丙氨酸且肽段中疏水性氨基酸的含量占比达50%以上。该结果可为深入开发菜籽饼蛋白资源提供理论参考。

Protective effects of tilapia(Oreochromis niloticus)skin gelatin hydrolysates on osteoporosis rats induced by retinoic acid

Tilapia skin gelatin hydrolysates(TSGH)were obtained by complex protease hydrolysis.The amino acid sequences of 50 peptides in TSGH were identified,and most of these peptides were found to contain the-Gly-Pro-sequence.The osteoporosis(OP)rat model induced by retinoic acid was prepared,and the effects of different doses of TSGH on OP in vivo were evaluated.Serum calcium(Ca)and phosphate(P),alkaline phosphatase activity,and osteocalcin levels in OP rats were regulated by TSGH.The bone length,dry weight index,maximum load,and Ca content of OP rats significantly increased by treatment TSGH in a dosedependent manner.Micro-CT images of the femurs and tibias of the rats indicated that the bone mineral density,cortical bone thickness,and cortical/trabecular bone area ratios were recovered and that OP symptoms were improved.Tartrate-resistant acid phosphate and hematoxylin-eosin staining showed that osteoclast numbers and histomorphological changes in the femurs in OP rats could be recovered by TSGH.

Analysis of the N Protein Sequence Variability in 13 Isolated PRRSV Strains from China

Object: To analyze porcine reproductive and respiratory syndrome virus (PRRSV) strains from 13 infection cases via the N protein gene and its encoded amino acid sequence and to provide a theoretical basis for the epidemiological study, prevention and control of porcine reproductive and respiratory syndrome (PRRS). Methods: In clinically suspected PRRSV infections, viruses were isolated by extracting viral nucleic acid and amplifying the N protein gene by RT-PCR. Then, the product was purified and sequenced to acquire the whole gene sequence of the N protein and its encoded amino acid sequence. DNASTAR software was used to analyze the homology, the genetic evolution and the derivation of the variability of amino acids of the N protein gene from 13 PRRSV strains and classical domestic and foreign strains. Results: Among the thirteen strains of PRRSV isolated from this study, ten strains had the greatest homology with the JXA1 strain (98.9% - 100%), and they belonged to the sublineage 8.7. The remaining three strains had the greatest homology with the NADC30 strain (95.4% - 97.1%), and they belonged to lineage one. The analysis of the variability of N protein amino acids showed that there were high frequency mutations in the five loci of 13 isolated strains of PRRSV as follows: 15th amino acid (10/13), 46th amino acid (11/13), 91st amino acid (10/13), 109th amino acid (10/13), and 117th amino acid (10/13). Conclusion: In recent years, sublineage 8.7 was the dominant pedigree in field PRRSV epidemic strains in China with lineage one occupying a certain proportion of the field. Four high frequency mutations existed in N protein antigen epitopes of isolated strains from the region. The nuclear localization signal (NLS) structure, specifically the 46th amino acid residue of the N protein, was mutated and genetically stable.

紫苏粕蛋白抗氧化活性肽的制备、分离纯化及序列鉴定

以紫苏粕粉为原料,使用碱性蛋白酶进行水解反应,制备抗氧化活性肽。采用超滤离心、凝胶过滤色谱和反相色谱等分离纯化手段对其富集。结果表明,相较其它3种蛋白酶,碱性蛋白酶Alcalase对紫苏粕蛋白的水解程度最高,约为(25.94±0.21)%;碱性蛋白酶作用紫苏蛋白后的酶解产物的抗氧化活性最好,对DPPH自由基清除率约为(91.01±0.73)%。酶解上清液经超滤离心分离后最小分子质量组分F1(小于3 ku)抗氧化活性最强,F1组分经Sephadex G-25凝胶过滤色谱分离后按照分子质量大小依次得到P1、P2、P33个组分,其中分子质量最小的P3组分抗氧化活性最高。P3组分经反相色谱分离所得4个组分中,最后被洗脱出来的P3-4组分疏水性最强且DPPH·自由基清除率最高,质量浓度为3μg/mL的P3-4溶液的DPPH·清除率为(58.8±0.78)%。通过LC-MS-MS鉴定,抗氧化活性肽P3-4组分为十二肽,其氨基酸序列为Lys-Leu-Lys-Asp-Ser-Phe-Glu-Arg-Gln-Gly-Met-Val,分子质量为1437.8 u。本研究结果为深入开发紫苏蛋白资源,研发紫苏抗氧化活性肽提供理论参考。

蟹肉酶解物的鲜味物质分析及鲜味肽的鉴定

以中华绒螯蟹为研究对象,以水解度和感官评价为评价指标,采用木瓜蛋白酶、胰蛋白酶、复合风味酶、中性蛋白酶、碱性蛋白酶、胃蛋白酶对蟹肉进行酶解,利用氨基酸分析仪和高效液相色谱技术对酶解物中呈味物质进行分析,经分离纯化后采用高效液相色谱串联质谱技术鉴定酶解物中鲜味肽的氨基酸序列。结果表明,中华绒螯蟹蟹肉经胰蛋白酶酶解后,鲜味浓郁、感官评价最佳,其水解度为(51.99±1.85)%,其中鲜味肽、谷氨酸、5’-单磷酸肌苷(5’-inosine monophosphate,5’-IMP)和琥珀酸是主要鲜味物质,经质谱鉴定及鲜味预测筛选,得到14条分子量为795.42~981.55 Da的具有潜在鲜味特性的肽。

多聚酸性氨基酸对蛋白质性质的影响

选取碳末端富含酸性氨基酸的拟南芥SnRK2.6(sucrose non-fermenting1-related protein kinase 2.6)和人源PDI(protein disulfide isomerase),以及近球形蛋白拟南芥PYL10 (PYR like protein 10),分别将重复酸性氨基酸序列添加到SnRK2.6(1-332)、PDI(1-440)、PYL10碳末端,利用大肠杆菌BL21重组表达,经过亲和层析,离子交换层析和分子排阻层析进行纯化,综合利用分析超速离心技术,分子排阻层析技术以及多角度静态光散射技术,研究人为设计的多聚氨基酸末端对蛋白质分子排阻行为,聚合状态和其他水力学性质的影响。结果发现,多聚酸性氨基酸末端虽不影响蛋白质分子的聚合状态,但会明显减少分子排阻色谱中蛋白质的洗脱体积,影响蛋白质分子的斯托克斯半径和轴长比等水力学性质。

Alterations in gut microbiota are related to metabolite profiles in spinal cord injury

Studies have shown that gut microbiota metabolites can enter the central nervous system via the blood-spinal cord barrier and cause neuroinflammation, thus constituting secondary injury after spinal cord injury. To investigate the correlation between gut microbiota and metabolites and the possible mechanism underlying the effects of gut microbiota on secondary injury after spinal cord injury, in this study, we established mouse models of T8–T10 traumatic spinal cord injury. We used 16 S rRNA gene amplicon sequencing and metabolomics to reveal the changes in gut microbiota and metabolites in fecal samples from the mouse model. Results showed a severe gut microbiota disturbance after spinal cord injury, which included marked increases in pro-inflammatory bacteria, such as Shigella, Bacteroides, Rikenella, Staphylococcus, and Mucispirillum and decreases in anti-inflammatory bacteria, such as Lactobacillus, Allobaculum, and Sutterella. Meanwhile, we identified 27 metabolites that decreased and 320 metabolites that increased in the injured spinal cord. Combined with pathway enrichment analysis, five markedly differential amino acids(L-leucine, L-methionine, L-phenylalanine, L-isoleucine and L-valine) were screened out, which play a pivotal role in activating oxidative stress and inflammatory responses following spinal cord injury. Integrated correlation analysis indicated that the alteration of gut microbiota was related to the differences in amino acids, which suggests that disturbances in gut microbiota might participate in the secondary injury through the accumulation of partial metabolites that activate oxidative stress and inflammatory responses. Findings from this study provide a new theoretical basis for improving the secondary injury after spinal cord injury through fecal microbial transplantation.

面筋蛋白咸味肽的分离纯化及结构鉴定

为了明确面筋蛋白酶解液中的咸味肽序列,对面筋蛋白咸味酶解物进行了分离纯化和结构鉴定。对酶解物脱盐处理后进行分离纯化。经过分级超滤,选择咸味最高、分子质量1 000 Da以下的组分进行葡聚糖凝胶Sephadex G-15过滤层析,结合感官评定结果筛选出咸味最强的组分,并利用液相色谱-串联质谱鉴定其氨基酸序列。最终得到面筋蛋白中咸味肽氨基酸序列为PFGQQ、PFSPQ、QPFP、PDFP、FDDP,分子质量分别为576.28、575.28、488.25、475.22、493.21 Da。本研究为面筋蛋白咸味肽的开发提供了一定理论依据。

斑马鱼肌肉Smyd1a蛋白生物信息学分析

通过使用美国国立生物技术信息中心(National Center for Biotechnology Information,NCBI)数据库检索出斑马鱼Smyd1a蛋白的氨基酸序列信息,对其进行生理生化分析,并通过NBCI寻找同源性高的不同种属的序列构建系统发育树。结果显示:Smyd1a蛋白的氨基酸数量是489个,分子量是56376.54 Da,理论等电点是6.27;该蛋白中,谷氨酸(Glu)含量最高,为8.4%;其次是亮氨酸(Leu)和丙氨酸(Ala),分别为8.0%和7.0%。Smyd1a是一个亲水蛋白质,但不是一个分泌蛋白,不存在跨膜螺旋区域,存在蛋白质的无规则卷曲等构象,Smyd1a属于SET家族。

氨基酸序列分析揭示苯丙氨酸/酪氨酸解氨酶在陆生植物中的演化

苯丙烷途径在陆生植物木质素和黄酮类等次生代谢产物的生物合成中至关重要,其中一步必需的反应为苯丙氨酸解氨酶(PAL)将底物苯丙氨酸脱氨催化为下游产物。最新研究发现了能够同时催化苯丙氨酸和酪氨酸脱氨的双功能酶—苯丙氨酸/酪氨酸解氨酶(PTAL),但是对诸多问题,例如怎样精准鉴定PAL和PTAL、植物PAL演化为PTAL其氨基酸序列发生了什么变异、哪些植物类群包含PTAL等,仍属未知。为了探索PAL基因家族在陆生植物中的演化及关键变异,本研究利用PAL氨基酸全长序列构建了陆生植物不同类群系统发育树,比较和分析了PAL和PTAL氨基酸序列并模拟了其蛋白质三维构象。结果表明,以PAL氨基酸序列构建的系统发育树,能够反映已知陆生植物的系统关系,PAL和PTAL的氨基酸序列中存在着8个差异位点,其中121和123这两个关键位点非常稳定,可以联合用于准确鉴定包含PAL和PTAL的植物,并且I121L和F123H的关键变异,导致只能与苯丙氨酸结合的PAL演化为也能同时结合酪氨酸的PTAL。

Amino Acid Sequence of an Excitatory Insect-selective Toxin (BmK IT) From Venom of the Scorpion Buthus martensi Karsch

The insect-selective neurotoxin(BmK IT) of scorpion Buthus martensi Karsch was first reduced and S-alkylated, and then digested by TPCK-trypsin and Staphylococcus aureus V-8 Protease. The enzymatic peptides were purified on TLC-plastic sheet and submitted to determine their amino acid compositions and sequences. The sequence of the 70 amino acid residues of BmK IT was established with reference to the primary structure of AaH IT, another excitatory insect-selective toxin from the venom of North African scorpion Androctonus australis Hector. About 75% of the homologous sequence was found in the molecules of BmK IT and AaH IT. It is obvious that the results contribute toward better understanding of the molecular structure characteristics, structure/activity relationship of scorpion insect-selective toxins, and they can serve as the molecular basis for utilizing the toxins as a tool to clarify molecular mechanism involved in channel gating, and to infer the possibility of developing them as new selective

18种观赏植物F3′5′H基因生物信息学分析

采用生物信息学方法对18种观赏植物类黄酮-3′5′-羟化酶基因(flavonoid-3′5′-hydroxylase,F3′5′H)的mRNA和氨基酸序列的理化性质、跨膜结构域、保守结构域、亚细胞定位、二级结构、三级结构和同源性进行预测与分析。结果表明,绝大多数观赏植物的F3′5′H为亲水性稳定蛋白质,以α螺旋为主、无信号肽的跨膜蛋白质;大多数定位于内质网膜上;其三级结构模型为5ylw.1.A铁锈醇合成酶,为单链蛋白,属于细胞色素P450基因家族;同源保守氨基酸序列为“LPPGP”“AGTDTS”和“PFGAGRRICAG”。