To identify the genomic species of Neisseria gonorrhoeae, evaluate the difference between two molecular epidemiological methods and examine the relationship between sex partners and genotypes of bacteria, 24 strains o...To identify the genomic species of Neisseria gonorrhoeae, evaluate the difference between two molecular epidemiological methods and examine the relationship between sex partners and genotypes of bacteria, 24 strains of Neisseria gonorrhoeae isolated from the outpatients with gonorrhea were identified by using the Opa genotyping and NG-MAST genotyping and the relationship between genotypes and phenotypes was studied. Twenty-four strains of Neisseria gonorrhoeae fell into 10 ST genotypes by NG-MAST genotyping, whereas these strains were classified into 12 OT Opa genotypes by Opa genotyping. A new epidemic strain of ST genotype （217-86% homologisation 178） in China was identified. It is concluded that genotypes of each pair of strains from a pair of patient/sex partner besides 45/46 are the same, indicating that contagious infection take place between patient and the sex partner. Opa genotyping was more effective than NG-MAST genotyping in identifying the genomic species of Neisseria gonorrhoeae. ST genotype could be further classified into different Opa-types.展开更多
The new spreading conchospores of Porphyra yezoensis, P. haitanensis and P. katadai var. hemiphylla are induced by N-Methy-N’-Nitro-N-Nitrosoguanidine (NG), a ⑴strong mutagen to induce the mutant of chromatophore. I...The new spreading conchospores of Porphyra yezoensis, P. haitanensis and P. katadai var. hemiphylla are induced by N-Methy-N’-Nitro-N-Nitrosoguanidine (NG), a ⑴strong mutagen to induce the mutant of chromatophore. It is showed that no mutants have ⑵been investigated in all control group. The conchospores of all the three species of Porphyra are easy to be induced by NG for they are in the stage of breeding, In the lowest dose (10 μg/mL) of this experiment, the mutant rate of P. yezoensis is up to 57.9%, that of P. haitanensis 30.7% and that of P. katadai var. hemiphylla ⑶51.7%. The mutant rate can not increase obviously by increasing the reagent concentration or prolonging the induction time. Within the scope of experiment, the inducement effects of three species of Porphyra are much similar, and the mutant rate is near 50%. The optimum induction concentration of NG is 10 μg/mL, and the optimum induction time is 30 minutes.展开更多
Distribution networks in China and several other countries are predominantly neutral inefficiently grounding systems(NIGSs),and more than 80%of the faults in distribution networks are single-phase-to-ground(SPG)faults...Distribution networks in China and several other countries are predominantly neutral inefficiently grounding systems(NIGSs),and more than 80%of the faults in distribution networks are single-phase-to-ground(SPG)faults.Because of the weak fault current and imperfect monitoring equipment configurations,methods used to determine the faulty line secti ons with SPG faults in NIGSs are in effective.The developme nt and application of distributi on-level phasor measurement units(PMUs)provide further comprehensive fault information for fault diagnosis in a distribution network.When an SPG fault occurs,the transient energy of the faulted line section tends to be higher than the sum of the transient energies of other line sections.In this regard,transient energy-based fault location algorithms appear to be a promising resolution.In this study,a field test plan was designed and implemented for a 10 kV distribution network.The test results dem on strate the effective ness of the transient en ergy-based SPG locati on method in practical distributi on networks.展开更多
The young thalli of Porphyra yezoensis and P.haitanensis are induced by N-Methy-N’-Nitro-N-Nitrosoguanidine (NG), a strong mutagen to induce the mutant of chromatophore. It is showed that no mutants have been investi...The young thalli of Porphyra yezoensis and P.haitanensis are induced by N-Methy-N’-Nitro-N-Nitrosoguanidine (NG), a strong mutagen to induce the mutant of chromatophore. It is showed that no mutants have been investigated in all control groups, while in the induced groups, it is easy to find mutant cells or mutant cell masses in the thallus. The pigmentation mutants may be the result of NG inducement. The mutant rate increases obviously with the mutagen concentration or by prolonging the induction time. Within the scope of experiment, the inducement effects of Porphyra yezoensis are raised from 11.2% to 28.7%, and those of P. haitanensis from 10.1% to 20.2%. According to our experiments, the amount of mutant cells in the same area does not increase with the induction intensity The amount of mutant cells in every group of inducement of both species of Porphyraμ changes very little with the fixed fields check method. The lowest dose (25g/mL) of this experiment has a high mutant rate. The mutant rate shows the percentage of mutant cells in all the survived cells, while the amount of mutant cells reflects the effect of μinducement. Thus the optimum induction concentration of NG is 25 g/mL, and the optimum induction time is 30 minutes.展开更多
<b>Introduction:</b> <i>Neisseria</i> is a large genus of bacteria that colonize mucosal surfaces. Of the 11 species that colonize humans, only two are pathogens, <i>N. meningitidis</i...<b>Introduction:</b> <i>Neisseria</i> is a large genus of bacteria that colonize mucosal surfaces. Of the 11 species that colonize humans, only two are pathogens, <i>N. meningitidis</i> and <i>N. gonorrhoeae</i>. Both are obligate human pathogens;the last is causal agent of gonorrhea disease. Although curable with timely antibiotic treatment, an annual incidence of more than 62 million cases is estimated by the World Health Organization and there are no successful vaccines available. In contrast, several prophylactic vaccine options for <i>Neisseria meningitidis</i> meningitis do exist. Of note, there is trace of cross parenteral response induced between <i>N. meningitidis</i> and <i>N. gonorrhoeae</i>, and Proteoliposome (PL, also named outer membrane vesicles, OMV) vaccine has shown high impact on this response. <b>Objective:</b> To determine effect of VAMENGOC-BC? and its derivates (AFPL1 and AFCo1) at mucosal and systemic level and possible cross response against <i>Neisseria</i> <i>gonhorroeae</i> in Balb/c and C57Bl/6 mice. <b>Methods: </b>We evaluated cross response against <i>N. gonorrhoeae</i> in mouse serum IgG and saliva IgA after mucosal immunization with VA-MENGOC-BC or its derivatives (AF, Adjuvant Finlay PL1 or AFCo (cochleate) 1). <b>Results: </b>Immunizations with AFPL1 or AFCo1 induce anti <i>N. gonorrhoeae</i> at saliva IgA and serum IgG responses similar to VA-MENGOC-BC? vaccine. <b>Conclusions: </b>Such data confirms a new possible window of prime-boost vaccination strategy against gonorrhea and extends our knowledge regarding the effect of PL vaccines on cross responses.展开更多
在淋病奈瑟菌(Neisseria gonorrhoeae,NG)6种保守外膜蛋白中筛选优势表位,设计淋病奈瑟菌多表位亚单位疫苗并模拟其免疫效果。首先,通过C-ImmSim在线服务器对6种外膜蛋白进行计算机免疫模拟,并分别从细胞毒性T细胞(cytotoxic T lymphocy...在淋病奈瑟菌(Neisseria gonorrhoeae,NG)6种保守外膜蛋白中筛选优势表位,设计淋病奈瑟菌多表位亚单位疫苗并模拟其免疫效果。首先,通过C-ImmSim在线服务器对6种外膜蛋白进行计算机免疫模拟,并分别从细胞毒性T细胞(cytotoxic T lymphocyte,CTL)、辅助性T细胞(helper T cell,Th cell)及B细胞免疫反应强的外膜蛋白中筛选用于构建多表位亚单位疫苗的优势CTL表位、Th细胞表位和B细胞表位。其次,在对各优势表位进行抗原性、致敏性和毒性分析后,将不同排列组合的优势表位与抗菌肽及PADRE序列连接起来,设计出多表位亚单位疫苗。再次,对所设计的各种多表位亚单位疫苗进行计算机免疫模拟,以评价其体液免疫和细胞免疫的效果。最后,对筛选出的免疫效果较好的候选亚单位疫苗进行抗原性、致敏性、理化性质、溶解性评估以及二级结构分析,以确保候选亚单位疫苗符合疫苗设计标准。结果显示,筛选出符合要求的优势候选CTL表位8个、Th细胞表位10个和B细胞表位14个;共设计出64种多表位亚单位疫苗,其中3种亚单位疫苗的免疫模拟结果最佳,均满足抗原性强、对人体无致敏性及无细胞毒性的要求,且均为亲水性、耐热性较好的稳定蛋白质,同时能在大肠杆菌中实现可溶性的过表达,与抗体的亲和性也较强,符合疫苗设计的标准。研究结果表明,以C-ImmSim为主的生物信息学预测方法在多表位亚单位疫苗的反向设计及其免疫效果的高通量初步筛选方面具有良好的应用前景。本研究为淋病奈瑟菌疫苗研发提供了一种新的有效途径。展开更多
基金the National Natural Science Foundation of China (No. 30700717 and No. 30371293)New Teacher Foundation of Ministry of Education of China (No. 20070487140)
文摘To identify the genomic species of Neisseria gonorrhoeae, evaluate the difference between two molecular epidemiological methods and examine the relationship between sex partners and genotypes of bacteria, 24 strains of Neisseria gonorrhoeae isolated from the outpatients with gonorrhea were identified by using the Opa genotyping and NG-MAST genotyping and the relationship between genotypes and phenotypes was studied. Twenty-four strains of Neisseria gonorrhoeae fell into 10 ST genotypes by NG-MAST genotyping, whereas these strains were classified into 12 OT Opa genotypes by Opa genotyping. A new epidemic strain of ST genotype （217-86% homologisation 178） in China was identified. It is concluded that genotypes of each pair of strains from a pair of patient/sex partner besides 45/46 are the same, indicating that contagious infection take place between patient and the sex partner. Opa genotyping was more effective than NG-MAST genotyping in identifying the genomic species of Neisseria gonorrhoeae. ST genotype could be further classified into different Opa-types.
基金Supported by NSFCC39770593 EMBLC CAS and Applied Fundamental Project of Jiangsu Province (BJ95182). National Key Fundamental Research Program (G1999012009).
文摘The new spreading conchospores of Porphyra yezoensis, P. haitanensis and P. katadai var. hemiphylla are induced by N-Methy-N’-Nitro-N-Nitrosoguanidine (NG), a ⑴strong mutagen to induce the mutant of chromatophore. It is showed that no mutants have ⑵been investigated in all control group. The conchospores of all the three species of Porphyra are easy to be induced by NG for they are in the stage of breeding, In the lowest dose (10 μg/mL) of this experiment, the mutant rate of P. yezoensis is up to 57.9%, that of P. haitanensis 30.7% and that of P. katadai var. hemiphylla ⑶51.7%. The mutant rate can not increase obviously by increasing the reagent concentration or prolonging the induction time. Within the scope of experiment, the inducement effects of three species of Porphyra are much similar, and the mutant rate is near 50%. The optimum induction concentration of NG is 10 μg/mL, and the optimum induction time is 30 minutes.
基金National Key R&D Program of China(2017YFB0902800)Science and Technology Project of State Grid Corporation of China(52094017003D)supported this work.
文摘Distribution networks in China and several other countries are predominantly neutral inefficiently grounding systems(NIGSs),and more than 80%of the faults in distribution networks are single-phase-to-ground(SPG)faults.Because of the weak fault current and imperfect monitoring equipment configurations,methods used to determine the faulty line secti ons with SPG faults in NIGSs are in effective.The developme nt and application of distributi on-level phasor measurement units(PMUs)provide further comprehensive fault information for fault diagnosis in a distribution network.When an SPG fault occurs,the transient energy of the faulted line section tends to be higher than the sum of the transient energies of other line sections.In this regard,transient energy-based fault location algorithms appear to be a promising resolution.In this study,a field test plan was designed and implemented for a 10 kV distribution network.The test results dem on strate the effective ness of the transient en ergy-based SPG locati on method in practical distributi on networks.
基金Supported by NSFCC39770593 EMBLC+1 种基金 CAS and Applied Fundamental Project of Jiangsu Province (BJ95182). National Key Fundamental Research Program (G1999012009).
文摘The young thalli of Porphyra yezoensis and P.haitanensis are induced by N-Methy-N’-Nitro-N-Nitrosoguanidine (NG), a strong mutagen to induce the mutant of chromatophore. It is showed that no mutants have been investigated in all control groups, while in the induced groups, it is easy to find mutant cells or mutant cell masses in the thallus. The pigmentation mutants may be the result of NG inducement. The mutant rate increases obviously with the mutagen concentration or by prolonging the induction time. Within the scope of experiment, the inducement effects of Porphyra yezoensis are raised from 11.2% to 28.7%, and those of P. haitanensis from 10.1% to 20.2%. According to our experiments, the amount of mutant cells in the same area does not increase with the induction intensity The amount of mutant cells in every group of inducement of both species of Porphyraμ changes very little with the fixed fields check method. The lowest dose (25g/mL) of this experiment has a high mutant rate. The mutant rate shows the percentage of mutant cells in all the survived cells, while the amount of mutant cells reflects the effect of μinducement. Thus the optimum induction concentration of NG is 25 g/mL, and the optimum induction time is 30 minutes.
文摘<b>Introduction:</b> <i>Neisseria</i> is a large genus of bacteria that colonize mucosal surfaces. Of the 11 species that colonize humans, only two are pathogens, <i>N. meningitidis</i> and <i>N. gonorrhoeae</i>. Both are obligate human pathogens;the last is causal agent of gonorrhea disease. Although curable with timely antibiotic treatment, an annual incidence of more than 62 million cases is estimated by the World Health Organization and there are no successful vaccines available. In contrast, several prophylactic vaccine options for <i>Neisseria meningitidis</i> meningitis do exist. Of note, there is trace of cross parenteral response induced between <i>N. meningitidis</i> and <i>N. gonorrhoeae</i>, and Proteoliposome (PL, also named outer membrane vesicles, OMV) vaccine has shown high impact on this response. <b>Objective:</b> To determine effect of VAMENGOC-BC? and its derivates (AFPL1 and AFCo1) at mucosal and systemic level and possible cross response against <i>Neisseria</i> <i>gonhorroeae</i> in Balb/c and C57Bl/6 mice. <b>Methods: </b>We evaluated cross response against <i>N. gonorrhoeae</i> in mouse serum IgG and saliva IgA after mucosal immunization with VA-MENGOC-BC or its derivatives (AF, Adjuvant Finlay PL1 or AFCo (cochleate) 1). <b>Results: </b>Immunizations with AFPL1 or AFCo1 induce anti <i>N. gonorrhoeae</i> at saliva IgA and serum IgG responses similar to VA-MENGOC-BC? vaccine. <b>Conclusions: </b>Such data confirms a new possible window of prime-boost vaccination strategy against gonorrhea and extends our knowledge regarding the effect of PL vaccines on cross responses.
文摘在淋病奈瑟菌(Neisseria gonorrhoeae,NG)6种保守外膜蛋白中筛选优势表位,设计淋病奈瑟菌多表位亚单位疫苗并模拟其免疫效果。首先,通过C-ImmSim在线服务器对6种外膜蛋白进行计算机免疫模拟,并分别从细胞毒性T细胞(cytotoxic T lymphocyte,CTL)、辅助性T细胞(helper T cell,Th cell)及B细胞免疫反应强的外膜蛋白中筛选用于构建多表位亚单位疫苗的优势CTL表位、Th细胞表位和B细胞表位。其次,在对各优势表位进行抗原性、致敏性和毒性分析后,将不同排列组合的优势表位与抗菌肽及PADRE序列连接起来,设计出多表位亚单位疫苗。再次,对所设计的各种多表位亚单位疫苗进行计算机免疫模拟,以评价其体液免疫和细胞免疫的效果。最后,对筛选出的免疫效果较好的候选亚单位疫苗进行抗原性、致敏性、理化性质、溶解性评估以及二级结构分析,以确保候选亚单位疫苗符合疫苗设计标准。结果显示,筛选出符合要求的优势候选CTL表位8个、Th细胞表位10个和B细胞表位14个;共设计出64种多表位亚单位疫苗,其中3种亚单位疫苗的免疫模拟结果最佳,均满足抗原性强、对人体无致敏性及无细胞毒性的要求,且均为亲水性、耐热性较好的稳定蛋白质,同时能在大肠杆菌中实现可溶性的过表达,与抗体的亲和性也较强,符合疫苗设计的标准。研究结果表明,以C-ImmSim为主的生物信息学预测方法在多表位亚单位疫苗的反向设计及其免疫效果的高通量初步筛选方面具有良好的应用前景。本研究为淋病奈瑟菌疫苗研发提供了一种新的有效途径。
