猪繁殖与呼吸综合征(Porcine Reproductive and Respiratory Syndrome,PRRS),俗称猪蓝耳病,是由猪繁殖与呼吸综合征病毒(Porcine Reproductive and Respiratory Syndrome Virus,PRRSV)引的一种猪繁殖和呼吸系统障碍的传染病。我国蓝耳...猪繁殖与呼吸综合征(Porcine Reproductive and Respiratory Syndrome,PRRS),俗称猪蓝耳病,是由猪繁殖与呼吸综合征病毒(Porcine Reproductive and Respiratory Syndrome Virus,PRRSV)引的一种猪繁殖和呼吸系统障碍的传染病。我国蓝耳病主要流行虽仍以经典毒株、高致病性毒株为主,但NADC30-like、NADC34-like、QYYZ-like等毒株的流行呈现逐渐上升趋势。目前我国仍未针对NADC30-like(及NADC34-like)毒株推出具有免疫保护的商品化疫苗,且相关基础研究的报道仍较少,造成NADC30-like的田间流行较难防控;现就类NADC30毒株在全球范围内关于致病性、商品疫苗保护力、疫苗研发进展等方面的研究进展进行整理,为猪场防治类NADC30毒株提供基础数据和防控思路。展开更多
猪繁殖与呼吸综合征(Porcine reproductive and respiratory syndrome,PRRS)是目前严重威胁养猪业发展的重要疫病之一,主要引起母猪繁殖障碍和仔猪呼吸道疾病。1987年美国首次报道该病以来,其毒株不断发生演变。2012年我国出现新的类NAD...猪繁殖与呼吸综合征(Porcine reproductive and respiratory syndrome,PRRS)是目前严重威胁养猪业发展的重要疫病之一,主要引起母猪繁殖障碍和仔猪呼吸道疾病。1987年美国首次报道该病以来,其毒株不断发生演变。2012年我国出现新的类NADC30毒株(NADC30-like毒株),该毒株可导致母猪流产及仔猪死淘率升高,给养猪业带来新的威胁,因此了解NADC30-like毒株在我国的流行情况及防控措施,对保障我国生猪健康养殖具有重要意义。展开更多
Porcine reproductive and respiratory syndrome(PRRS)is one of the most significant diseases affecting the pig industry worldwide.The PRRSV mutation rate is the highest among the RNA viruses.To date,NADC30-like PRRSV an...Porcine reproductive and respiratory syndrome(PRRS)is one of the most significant diseases affecting the pig industry worldwide.The PRRSV mutation rate is the highest among the RNA viruses.To date,NADC30-like PRRSV and highly pathogenic PRRSV(HP-PRRSV)are the dominant epidemic strains in China;however,commercial vaccines do not always provide sufficient cross-protection,and the reasons for insufficient protection are unclear.This study isolated a wild-type NADC30-like PRRSV,SX-YL1806,from Shaanxi Province.Vaccination challenge experiments in piglets showed that commercial modified live virus(MLV)vaccines provided good protection against HP-PRRSV.However,it could not provide sufficient protection against the novel strain SXYL1806.To explore the reasons for this phenomenon,we compared the genomic homology between the MLV strain and HP-PRRSV or NADC30-like PRRSV and found that the MLV strain had a lower genome similarity with NADC30-like PRRSV.Serum neutralization assay showed that MLV-immune serum slightly promoted the homologous HP-PRRSV replication and significantly promoted the heterologous NADC30-like PRRSV strain replication in vitro,suggesting that antibody-dependent enhancement(ADE)might also play a role in decreasing MLV protective efficacy.These findings expand our understanding of the potential factors affecting the protective effect of PRRSV MLV vaccines against the NADC30-like strains.展开更多
文摘猪繁殖与呼吸综合征(Porcine Reproductive and Respiratory Syndrome,PRRS),俗称猪蓝耳病,是由猪繁殖与呼吸综合征病毒(Porcine Reproductive and Respiratory Syndrome Virus,PRRSV)引的一种猪繁殖和呼吸系统障碍的传染病。我国蓝耳病主要流行虽仍以经典毒株、高致病性毒株为主,但NADC30-like、NADC34-like、QYYZ-like等毒株的流行呈现逐渐上升趋势。目前我国仍未针对NADC30-like(及NADC34-like)毒株推出具有免疫保护的商品化疫苗,且相关基础研究的报道仍较少,造成NADC30-like的田间流行较难防控;现就类NADC30毒株在全球范围内关于致病性、商品疫苗保护力、疫苗研发进展等方面的研究进展进行整理,为猪场防治类NADC30毒株提供基础数据和防控思路。
文摘猪繁殖与呼吸综合征(Porcine reproductive and respiratory syndrome,PRRS)是目前严重威胁养猪业发展的重要疫病之一,主要引起母猪繁殖障碍和仔猪呼吸道疾病。1987年美国首次报道该病以来,其毒株不断发生演变。2012年我国出现新的类NADC30毒株(NADC30-like毒株),该毒株可导致母猪流产及仔猪死淘率升高,给养猪业带来新的威胁,因此了解NADC30-like毒株在我国的流行情况及防控措施,对保障我国生猪健康养殖具有重要意义。
基金This research was supported by the National Natural Science Foundation of China(32172846)the Earmarked Fund for CARS-35,the Science and Technology Major Project of Gansu Province(22ZD6NA001)+4 种基金the Science Foundation for Distinguished Young Scholars of Shaanxi Province(2021JC-18)the Natural Science Foundation of Gansu Province(23JRRA1153)the Science and Technology Plan Project of Gansu Province(23JRRA561)the Chinese Academy of Agricultural Science and Technology Innovation Project(CAAS-ASTIP-JBGS-20210602)the Strategic Priority Research Program of the National Center of Technology Innovation for Pigs(NCTIP-XD/C03).
文摘Porcine reproductive and respiratory syndrome(PRRS)is one of the most significant diseases affecting the pig industry worldwide.The PRRSV mutation rate is the highest among the RNA viruses.To date,NADC30-like PRRSV and highly pathogenic PRRSV(HP-PRRSV)are the dominant epidemic strains in China;however,commercial vaccines do not always provide sufficient cross-protection,and the reasons for insufficient protection are unclear.This study isolated a wild-type NADC30-like PRRSV,SX-YL1806,from Shaanxi Province.Vaccination challenge experiments in piglets showed that commercial modified live virus(MLV)vaccines provided good protection against HP-PRRSV.However,it could not provide sufficient protection against the novel strain SXYL1806.To explore the reasons for this phenomenon,we compared the genomic homology between the MLV strain and HP-PRRSV or NADC30-like PRRSV and found that the MLV strain had a lower genome similarity with NADC30-like PRRSV.Serum neutralization assay showed that MLV-immune serum slightly promoted the homologous HP-PRRSV replication and significantly promoted the heterologous NADC30-like PRRSV strain replication in vitro,suggesting that antibody-dependent enhancement(ADE)might also play a role in decreasing MLV protective efficacy.These findings expand our understanding of the potential factors affecting the protective effect of PRRSV MLV vaccines against the NADC30-like strains.
