Nicotinamide adenine dinucleotide (NADH/NAD+) is involved in important biochemical reactions in human metabolism, including participation in energy production by mitochondria. The changes in fluorescence intensity as ...Nicotinamide adenine dinucleotide (NADH/NAD+) is involved in important biochemical reactions in human metabolism, including participation in energy production by mitochondria. The changes in fluorescence intensity as a function of time in response to blocking and releasing of blood flow in a forearm are used as a measure of oxygen transport with blood to the tissue, which directly correlates with the skin microcirculation status. In this paper, a non-invasive dynamic monitoring system based on blood flow-mediated skin fluorescence (FMSF) technology is developed to monitor the NADH fluorescence intensity of skin tissue during the process of blocking reactive hyperemia. Simultaneously, laser speckle contrast imaging (LSCI) and laser Doppler flowmetry (LDF) were used to observe blood flow, blood oxygen saturation (SOt2) and relative amount of hemoglobin (rHb) during the measurement process, which helped to explore NADH dynamics relevant physiological changes. A variety of parameters have been derived to describe NADH fluorescence curve based on the FMSF device. The experimental results are conducive to understanding the NADH measurement and the physiological processes related to it, which help FMSF to be a great avenue for in vivo physiological, clinical and pharmacological research on mitochondrial metabolism.展开更多
以动物园为代表的圈养环境是开展野生动物迁地保护、繁育、科普宣传的重要场地,亦为开展科学研究等提供了宝贵资源。而圈养环境中的国家一级重点保护动物豹(Pantera pardus)的遗传资源则为了解其野生种群提供了窗口。本研究以郑州市动...以动物园为代表的圈养环境是开展野生动物迁地保护、繁育、科普宣传的重要场地,亦为开展科学研究等提供了宝贵资源。而圈养环境中的国家一级重点保护动物豹(Pantera pardus)的遗传资源则为了解其野生种群提供了窗口。本研究以郑州市动物园圈养的1只雌性豹为对象,通过提取该个体的基因组DNA并进行PCR扩增与测序,利用所获得的NADH5基因片段探究该个体属何豹亚种。经与NCBI数据库比对以及系统发生分析,本研究显示该个体属于豹华北亚种(P. p. fontanierii)。基于本研究,建议开展国内圈养豹遗传资源调查,以便系统地开展圈养豹的保护、繁育工作。展开更多
文摘Nicotinamide adenine dinucleotide (NADH/NAD+) is involved in important biochemical reactions in human metabolism, including participation in energy production by mitochondria. The changes in fluorescence intensity as a function of time in response to blocking and releasing of blood flow in a forearm are used as a measure of oxygen transport with blood to the tissue, which directly correlates with the skin microcirculation status. In this paper, a non-invasive dynamic monitoring system based on blood flow-mediated skin fluorescence (FMSF) technology is developed to monitor the NADH fluorescence intensity of skin tissue during the process of blocking reactive hyperemia. Simultaneously, laser speckle contrast imaging (LSCI) and laser Doppler flowmetry (LDF) were used to observe blood flow, blood oxygen saturation (SOt2) and relative amount of hemoglobin (rHb) during the measurement process, which helped to explore NADH dynamics relevant physiological changes. A variety of parameters have been derived to describe NADH fluorescence curve based on the FMSF device. The experimental results are conducive to understanding the NADH measurement and the physiological processes related to it, which help FMSF to be a great avenue for in vivo physiological, clinical and pharmacological research on mitochondrial metabolism.
文摘以动物园为代表的圈养环境是开展野生动物迁地保护、繁育、科普宣传的重要场地,亦为开展科学研究等提供了宝贵资源。而圈养环境中的国家一级重点保护动物豹(Pantera pardus)的遗传资源则为了解其野生种群提供了窗口。本研究以郑州市动物园圈养的1只雌性豹为对象,通过提取该个体的基因组DNA并进行PCR扩增与测序,利用所获得的NADH5基因片段探究该个体属何豹亚种。经与NCBI数据库比对以及系统发生分析,本研究显示该个体属于豹华北亚种(P. p. fontanierii)。基于本研究,建议开展国内圈养豹遗传资源调查,以便系统地开展圈养豹的保护、繁育工作。
