Lactobacillus are considered promising therapeutic methods for nonalcoholic fatty liver disease(NAFLD).The effects of two strains of Ltmosilactobacillus mucosae on NAFLD were investigated in this study.Fourweek-old ma...Lactobacillus are considered promising therapeutic methods for nonalcoholic fatty liver disease(NAFLD).The effects of two strains of Ltmosilactobacillus mucosae on NAFLD were investigated in this study.Fourweek-old male C57BL/6J mice were divided into 4 groups(n=8 per group,Control,Model,FZJTZ26M3,FGSYC17L3).L.mucosae FZJTZ26M3 reduced the mice 's body weight,liver weight,and adipose tissue weight after 12 weeks of therapy.According to serum analysis,total cholesterol,triacylglycerol,and low-density lipoprotein cholesterol significantly decreased after L.mucosae FZJTZ26M3 intervention.Liver pathology showed that L.mucosae FZJTZ26M3 was effective to ameliorate lipid deposition in NAFLD mice.Additionally,the expression of the gene related to lipid metabolism in the liver and adipose tissue was analyzed,and the results indicated that L.mucosae FZJTZ26M3 could alleviate NAFLD by regulating lipid metabolism.Furthermore,the results of 16S rRNA gene sequencing revealed a drop in the relative abundance of Ruminococcaceae,which is linked to inflammation,but the relative abundance of a potential probiotic Akkermansia significantly increased after L.mucosae FZJTZ26M3 intervention.Generally,L.mucosae FZJTZ26M3 could be a candidate to prevent NAFLD.展开更多
Nonalcoholic fatty liver disease (NAFLD) is the leading cause of liver disease in the Western world and has a strong relationship to obesity and diabetes. NAFLD has not been well studied in pregnant women. We studied ...Nonalcoholic fatty liver disease (NAFLD) is the leading cause of liver disease in the Western world and has a strong relationship to obesity and diabetes. NAFLD has not been well studied in pregnant women. We studied a series of lean, obese, and gestational diabetic pregnant women and determined that liver enzymes would not serve to diagnose the presence of NAFLD in an obstetric population. A total of 59 pregnant women of various gestational ages and maternal weights who denied a history of alcohol intake or preexisting liver disease were recruited from a single-center university general obstetric clinic. Pregnant women underwent a maternal abdominal and obstetrical ultrasound, and blood samples were obtained for assays of liver enzymes, adiponectin and leptin. The presence of hepatic steatosis was established using standardized ultrasound criteria. NAFLD was detected by ultrasound in 48.9% of pregnant women and almost equally distributed between lean and obese women. The incidence of NAFLD in gestational diabetic pregnancies (50%) was comparable to the non-diabetic group with NAFLD in pregnant women. Adiponectin and leptin are similar between gravidas with and without NAFLD. Screening of pregnant women at any stage of pregnancy, early or late, lean or obese, or gestational diabetes after 26 weeks of gestation, would serve as a useful approach to determine NAFLD in pregnant women.展开更多
基金supported by the National Natural Science Foundation of China (32021005, 31820103010)111 project (BP0719028)the Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province。
文摘Lactobacillus are considered promising therapeutic methods for nonalcoholic fatty liver disease(NAFLD).The effects of two strains of Ltmosilactobacillus mucosae on NAFLD were investigated in this study.Fourweek-old male C57BL/6J mice were divided into 4 groups(n=8 per group,Control,Model,FZJTZ26M3,FGSYC17L3).L.mucosae FZJTZ26M3 reduced the mice 's body weight,liver weight,and adipose tissue weight after 12 weeks of therapy.According to serum analysis,total cholesterol,triacylglycerol,and low-density lipoprotein cholesterol significantly decreased after L.mucosae FZJTZ26M3 intervention.Liver pathology showed that L.mucosae FZJTZ26M3 was effective to ameliorate lipid deposition in NAFLD mice.Additionally,the expression of the gene related to lipid metabolism in the liver and adipose tissue was analyzed,and the results indicated that L.mucosae FZJTZ26M3 could alleviate NAFLD by regulating lipid metabolism.Furthermore,the results of 16S rRNA gene sequencing revealed a drop in the relative abundance of Ruminococcaceae,which is linked to inflammation,but the relative abundance of a potential probiotic Akkermansia significantly increased after L.mucosae FZJTZ26M3 intervention.Generally,L.mucosae FZJTZ26M3 could be a candidate to prevent NAFLD.
文摘Nonalcoholic fatty liver disease (NAFLD) is the leading cause of liver disease in the Western world and has a strong relationship to obesity and diabetes. NAFLD has not been well studied in pregnant women. We studied a series of lean, obese, and gestational diabetic pregnant women and determined that liver enzymes would not serve to diagnose the presence of NAFLD in an obstetric population. A total of 59 pregnant women of various gestational ages and maternal weights who denied a history of alcohol intake or preexisting liver disease were recruited from a single-center university general obstetric clinic. Pregnant women underwent a maternal abdominal and obstetrical ultrasound, and blood samples were obtained for assays of liver enzymes, adiponectin and leptin. The presence of hepatic steatosis was established using standardized ultrasound criteria. NAFLD was detected by ultrasound in 48.9% of pregnant women and almost equally distributed between lean and obese women. The incidence of NAFLD in gestational diabetic pregnancies (50%) was comparable to the non-diabetic group with NAFLD in pregnant women. Adiponectin and leptin are similar between gravidas with and without NAFLD. Screening of pregnant women at any stage of pregnancy, early or late, lean or obese, or gestational diabetes after 26 weeks of gestation, would serve as a useful approach to determine NAFLD in pregnant women.
