Background: Pemphigoid gestationis (PG) is a rare pregnancyassociated subepidermal immunobullous disease that targets hemidesmosomal proteins, particularly BP180. Clinically, PG can resemble the eruption known as poly...Background: Pemphigoid gestationis (PG) is a rare pregnancyassociated subepidermal immunobullous disease that targets hemidesmosomal proteins, particularly BP180. Clinically, PG can resemble the eruption known as polymorphic urticarial papules and plaques of pregnancy (PUPPP), and accurate differentiation between these 2 pruritic pregnancy dermatoses has important implications for fetal and maternal prognoses. Results of epitope mapping studies show that IgG autoantibodies in up to 90%of PG serum samples target the well-defined membrane-proximal NC16a domain of BP180. Objective: To examine the usefulness of a commercially available NC16a domain enzyme-linked immunosorbent assay in the serodiagnosis of PG and in the differentiation of PG from PUPPP. Participants: A total of 412 women consisting of pretreatment patients with PG (n=82), patients with PUPPP (n=164), and age-and sex-matched controls (n=166). Methods: All serum samples were assayed in duplicate. Receiver operating characteristic analyses were performed to determine a cutoff value for the diagnosis of PG and for differentiation from PUPPP and controls. Results: A cutoff value of 10 enzyme-linked immunosorbent assay units was associated with specificity and sensitivity of 96%. Conclusions: The NC16a enzyme-linked immunosorbent assay is highly sensitive and highly specific in differentiating PG from PUPPP, and it is potentially a valuable tool in the serodiagnosis of PG.展开更多
Bullous pemphigoid (BP) is associated with autoantibodies to the 180-kDa BP antigen (BP180), and the antigenic site exists on noncollagenous 16a (NC16a) domain of BP180. We now report a male BP patient whose IgG autoa...Bullous pemphigoid (BP) is associated with autoantibodies to the 180-kDa BP antigen (BP180), and the antigenic site exists on noncollagenous 16a (NC16a) domain of BP180. We now report a male BP patient whose IgG autoantibodies did not react against the NC16a domain of BP180 by either immunoblotting or ELISA, whereas they did react with BP180 extracted from normal human keratinocytes. Anti-BP180 cicatricial pemphigoid was ruled out due to the lack of conjunctival mucosal involvement and the absence of scarring in the oral cavity. Our findings indicate that there is an antigenic reactive region other than NC16a on the extracellular domain of BP180. There have been few reports describing detailed clinical features of BP caused by autoantibodies targeting antigenic sites other than the NC16a domain. We conclude that it is difficult to differentiate their clinical features from those associated with autoantibodies targeting the NC16a domain of BP180.展开更多
It was proved by ICP, fluorescence spectra and N 2 adsorption that the rare earth complex [C 5H 5NC 16H 33] [Eu(TTA) 4] is in the channel of Si-MCM-41 in the course of assembly. The rare earth complex of 67.9% is in t...It was proved by ICP, fluorescence spectra and N 2 adsorption that the rare earth complex [C 5H 5NC 16H 33] [Eu(TTA) 4] is in the channel of Si-MCM-41 in the course of assembly. The rare earth complex of 67.9% is in the channel, suggesting that the assembly of the complex molecular on the mesoporous MCM-41 was carried out mainly in the channel.展开更多
A thin film electroluminescence cell with the structure of ITO /PPV /PVK∶Eu（TTA）4C5H5NC16H33∶PBD /Alq 3 /Al has been fabricated.Red emissio n with a very sharp spectral band at 614nm was observed and a maximum lum...A thin film electroluminescence cell with the structure of ITO /PPV /PVK∶Eu（TTA）4C5H5NC16H33∶PBD /Alq 3 /Al has been fabricated.Red emissio n with a very sharp spectral band at 614nm was observed and a maximum luminance of 20cd·m -2 at 36V was obtained from the spin-coated device.The full width at half maximum of lu-minescent spectrum is less than 10nm.展开更多
Background:Mucous membrane pemphigoid (MMP) is a chronic blistering skin dise ase frequently associated with circul atingautoantibodies directed to a number o f antigens including the NC16A region of BP180. NC16A doma...Background:Mucous membrane pemphigoid (MMP) is a chronic blistering skin dise ase frequently associated with circul atingautoantibodies directed to a number o f antigens including the NC16A region of BP180. NC16A domain-specific T cells h ave been identified in the blood of individuals with bullous pemphigoid (BP), pe mphigoid gestationis and linear IgA disease, but there are no data investigating the potential role for such T cells in the pathogenesis of MMP. Objectives:To test the hypothesis that NC16A-specific T cells exist in the peripheral blood o f individuals with MMP. Methods:We isolated peripheral blood mononuclear cells from 10 patients with MMP, 17 with BP and 10 healthy controls and examined the i mmunogenicity of overlapping peptides spanning the NC16A domain using interferon (IFN)-γenzyme-linked immunospot assay. Results:Significant IFN-γproductio n was observed in response to the NC16A peptides in two of the patients with MMP and two of the patients with BP but in none of the normal controls. These data suggest that in a minority of individuals with MMP, NC16A domain-specific T cel ls circulate at sufficiently high frequency to be detectable directly ex vivo an d to show rapid effector function. Conclusions:Overall, these findings are the first to examine the potential role for antigen-specific autoreactive T cells i n the pathogenesis of MMP, and confirm that in some individuals the NC16A domain may be an important target antigen.展开更多
文摘Background: Pemphigoid gestationis (PG) is a rare pregnancyassociated subepidermal immunobullous disease that targets hemidesmosomal proteins, particularly BP180. Clinically, PG can resemble the eruption known as polymorphic urticarial papules and plaques of pregnancy (PUPPP), and accurate differentiation between these 2 pruritic pregnancy dermatoses has important implications for fetal and maternal prognoses. Results of epitope mapping studies show that IgG autoantibodies in up to 90%of PG serum samples target the well-defined membrane-proximal NC16a domain of BP180. Objective: To examine the usefulness of a commercially available NC16a domain enzyme-linked immunosorbent assay in the serodiagnosis of PG and in the differentiation of PG from PUPPP. Participants: A total of 412 women consisting of pretreatment patients with PG (n=82), patients with PUPPP (n=164), and age-and sex-matched controls (n=166). Methods: All serum samples were assayed in duplicate. Receiver operating characteristic analyses were performed to determine a cutoff value for the diagnosis of PG and for differentiation from PUPPP and controls. Results: A cutoff value of 10 enzyme-linked immunosorbent assay units was associated with specificity and sensitivity of 96%. Conclusions: The NC16a enzyme-linked immunosorbent assay is highly sensitive and highly specific in differentiating PG from PUPPP, and it is potentially a valuable tool in the serodiagnosis of PG.
文摘Bullous pemphigoid (BP) is associated with autoantibodies to the 180-kDa BP antigen (BP180), and the antigenic site exists on noncollagenous 16a (NC16a) domain of BP180. We now report a male BP patient whose IgG autoantibodies did not react against the NC16a domain of BP180 by either immunoblotting or ELISA, whereas they did react with BP180 extracted from normal human keratinocytes. Anti-BP180 cicatricial pemphigoid was ruled out due to the lack of conjunctival mucosal involvement and the absence of scarring in the oral cavity. Our findings indicate that there is an antigenic reactive region other than NC16a on the extracellular domain of BP180. There have been few reports describing detailed clinical features of BP caused by autoantibodies targeting antigenic sites other than the NC16a domain. We conclude that it is difficult to differentiate their clinical features from those associated with autoantibodies targeting the NC16a domain of BP180.
文摘It was proved by ICP, fluorescence spectra and N 2 adsorption that the rare earth complex [C 5H 5NC 16H 33] [Eu(TTA) 4] is in the channel of Si-MCM-41 in the course of assembly. The rare earth complex of 67.9% is in the channel, suggesting that the assembly of the complex molecular on the mesoporous MCM-41 was carried out mainly in the channel.
文摘A thin film electroluminescence cell with the structure of ITO /PPV /PVK∶Eu（TTA）4C5H5NC16H33∶PBD /Alq 3 /Al has been fabricated.Red emissio n with a very sharp spectral band at 614nm was observed and a maximum luminance of 20cd·m -2 at 36V was obtained from the spin-coated device.The full width at half maximum of lu-minescent spectrum is less than 10nm.
文摘Background:Mucous membrane pemphigoid (MMP) is a chronic blistering skin dise ase frequently associated with circul atingautoantibodies directed to a number o f antigens including the NC16A region of BP180. NC16A domain-specific T cells h ave been identified in the blood of individuals with bullous pemphigoid (BP), pe mphigoid gestationis and linear IgA disease, but there are no data investigating the potential role for such T cells in the pathogenesis of MMP. Objectives:To test the hypothesis that NC16A-specific T cells exist in the peripheral blood o f individuals with MMP. Methods:We isolated peripheral blood mononuclear cells from 10 patients with MMP, 17 with BP and 10 healthy controls and examined the i mmunogenicity of overlapping peptides spanning the NC16A domain using interferon (IFN)-γenzyme-linked immunospot assay. Results:Significant IFN-γproductio n was observed in response to the NC16A peptides in two of the patients with MMP and two of the patients with BP but in none of the normal controls. These data suggest that in a minority of individuals with MMP, NC16A domain-specific T cel ls circulate at sufficiently high frequency to be detectable directly ex vivo an d to show rapid effector function. Conclusions:Overall, these findings are the first to examine the potential role for antigen-specific autoreactive T cells i n the pathogenesis of MMP, and confirm that in some individuals the NC16A domain may be an important target antigen.
