Lesion mmic mutants(LMMs)are plants that spontaneously form lesions without pathogeninfection or external stimulus and exhibit resistance to pathogens.Here,a rice LMM was created by ethylmethane sulfonate mutagenesis,...Lesion mmic mutants(LMMs)are plants that spontaneously form lesions without pathogeninfection or external stimulus and exhibit resistance to pathogens.Here,a rice LMM was created by ethylmethane sulfonate mutagenesis,named as hpil(hydrogen peroxide induced lesion).Diaminobenzidineand trypan blue staining showed that large amounts of H_(2)O_(2) were produced and cell death was occurredat and around the parts of lesion mimic in the rice leaves.The phenotype of hpil is controlled by a singlerecessive gene,localized at a 2 Mb interval on chromosome 2.The data suggested that hpil is a novelLMM with enhanced bacterial and fungal disease resistance,and multiple pathogenesis-related proteins(PRs)were up-regulated.The proteomes of leaves at three positions(different degrees of lesion mimicseverity)were characterized in hpil compared with its wild type plant.Differentially expressed proteinswere detected by two dimensional difference gel electrophoresis and 274 proteins were identified byMALDITOF/TOFTM.These proteins were related to metabolic process,cellular process and response tostimulus,with mostly down-regulated in hpil leaves.Many of these proteins were related to the Calvincycle,photosynthetic electron transport chain,glycolysis/gluconeogenesis and phosphonates pathways.Some resistance-related proteins including 14-3-3 proteins,OsPR10 and antioxidases such asperoxidase,superoxide dismutase and ascorbate peroxidase were up-regulated in leaves with lesionmimic.These results provide the foundation for cloning of the target gene and shed light on themechanism involved in autaimmunity of rice.展开更多
Combining the development status of information industry in China and based on theoretical discussions on labor demand and supply as well as the comprehensive analysis on endogenous technological progress and exogenou...Combining the development status of information industry in China and based on theoretical discussions on labor demand and supply as well as the comprehensive analysis on endogenous technological progress and exogenous educational quality, this paper concludes that the technological progress of information industry in China is skill- biased, i.e. technological progress of information industry increases the demand for skilled labor while decreases the demand for unskilled labor, and aggravates the wage difference between these two types of labor. Due to the effect of educational quality, the ratio of skilled labor employment to unskilled labor employment will reach a dynamic equilibrium and the wage difference between skilled labor and unskilled labor will not increase continuously. This paper then proves the foregoing conclusion with data of information industry in 26 provinces （autonomous region, municipality）. Focusing on typical skill-intensive information industry, this paper mainly studies the influence of educational quality, which not only complements the theoretical analysis on dual effects of technological progress on employment, but also provides feasible and practical basis to policies promoting the development of technological progress and full employment in China.展开更多
Abstract An electric circuit with dichotomous resistor is investigated. It is shown that the amplitude of the average electric current washing the resistor represents the phenomenon of stochastic resonance, which is t...Abstract An electric circuit with dichotomous resistor is investigated. It is shown that the amplitude of the average electric current washing the resistor represents the phenomenon of stochastic resonance, which is the response as a function of the correlation time of the dichotomous resistor.展开更多
ABN Armro(位于芝加哥的一家投资银行)预测，2000和2001年美国批准的新化学实体(NCE)各为16个，其中重磅炸弹级产品各为5个。伴随新产品带来潜在收益的是巨大的投资成本和高度的风险。目前，平均每5000个化合物只有一个最终成为商业上...ABN Armro(位于芝加哥的一家投资银行)预测，2000和2001年美国批准的新化学实体(NCE)各为16个，其中重磅炸弹级产品各为5个。伴随新产品带来潜在收益的是巨大的投资成本和高度的风险。目前，平均每5000个化合物只有一个最终成为商业上可行的处方药，而这需要投资5亿美元及10～15年的实验室工作、临床试验和范围广泛的法规评审过程。展开更多
基金supported by the Zhejiang Provincial Key Research and Development Plan(Grant No.2019C02006)the National Key Research and Development Program of China(Grant Nos.2016YFD0200804 and 2016YFD0100601-15)+2 种基金the Key Program of Zhejiang Provincial Foundation for Natural Science(Grant No.LZ16C130002)the Zhejiang Fundamental Public Welfare Research Program(Grant No.LGN19C140008)the State Key Laboratory for Managing Biotic and Chemical Threats to Quality and Safety of Agro-products(Grant No.2010DS700124-ZZ1907).
文摘Lesion mmic mutants(LMMs)are plants that spontaneously form lesions without pathogeninfection or external stimulus and exhibit resistance to pathogens.Here,a rice LMM was created by ethylmethane sulfonate mutagenesis,named as hpil(hydrogen peroxide induced lesion).Diaminobenzidineand trypan blue staining showed that large amounts of H_(2)O_(2) were produced and cell death was occurredat and around the parts of lesion mimic in the rice leaves.The phenotype of hpil is controlled by a singlerecessive gene,localized at a 2 Mb interval on chromosome 2.The data suggested that hpil is a novelLMM with enhanced bacterial and fungal disease resistance,and multiple pathogenesis-related proteins(PRs)were up-regulated.The proteomes of leaves at three positions(different degrees of lesion mimicseverity)were characterized in hpil compared with its wild type plant.Differentially expressed proteinswere detected by two dimensional difference gel electrophoresis and 274 proteins were identified byMALDITOF/TOFTM.These proteins were related to metabolic process,cellular process and response tostimulus,with mostly down-regulated in hpil leaves.Many of these proteins were related to the Calvincycle,photosynthetic electron transport chain,glycolysis/gluconeogenesis and phosphonates pathways.Some resistance-related proteins including 14-3-3 proteins,OsPR10 and antioxidases such asperoxidase,superoxide dismutase and ascorbate peroxidase were up-regulated in leaves with lesionmimic.These results provide the foundation for cloning of the target gene and shed light on themechanism involved in autaimmunity of rice.
基金This research is funded by the key project of National Social Science Foundation of China ＂Study on Major Issues and Countermeasures of Industrial Organization in China after the International Financial Crisis＂ （Approval No. 12AJY004） Major Research Projects on Humanities and Social Sciences of Shandong University ＂Study on Industrial Concentration, Enterprise Size and International Competitiveness of China＇s Manufacturing Industry against Economic Globalization＂ （Approval No.12RWZD15） Fund Project for Postgraduate＇s Independent Innovation of Shandong University ＂Study on the Impact Mechanism of IT Technical Progress on Employment.＂
文摘Combining the development status of information industry in China and based on theoretical discussions on labor demand and supply as well as the comprehensive analysis on endogenous technological progress and exogenous educational quality, this paper concludes that the technological progress of information industry in China is skill- biased, i.e. technological progress of information industry increases the demand for skilled labor while decreases the demand for unskilled labor, and aggravates the wage difference between these two types of labor. Due to the effect of educational quality, the ratio of skilled labor employment to unskilled labor employment will reach a dynamic equilibrium and the wage difference between skilled labor and unskilled labor will not increase continuously. This paper then proves the foregoing conclusion with data of information industry in 26 provinces （autonomous region, municipality）. Focusing on typical skill-intensive information industry, this paper mainly studies the influence of educational quality, which not only complements the theoretical analysis on dual effects of technological progress on employment, but also provides feasible and practical basis to policies promoting the development of technological progress and full employment in China.
基金The project supported by National Natural Science Foundation of China under Grant No, 10375009, and the Scientific Research Foundation for Research 0verseas Chinese Scholars of the State Education Ministry of China. and by K.C. Wong Magna Fund in Ningbo University
文摘Abstract An electric circuit with dichotomous resistor is investigated. It is shown that the amplitude of the average electric current washing the resistor represents the phenomenon of stochastic resonance, which is the response as a function of the correlation time of the dichotomous resistor.
