Objective:To detect the levels of TNF-αand NF-κB in serum and NF-κB in peripheral blood lymphocytes of patients with atrial fibrillation.To investigate the regularity and significance of its changes in patients wit...Objective:To detect the levels of TNF-αand NF-κB in serum and NF-κB in peripheral blood lymphocytes of patients with atrial fibrillation.To investigate the regularity and significance of its changes in patients with atrial fibrillation.Methods:Patients with atrial fibrillation hospitalized in our hospital from January 2020 to December 2021 were selected,including paroxysmal AF group(PAF group,n=75)and non-paroxysmal AF group(nPAF group,n=60),and healthy subjects were selected as the control group(CON group,n=107).Routine examination and color doppler echocardiography were improved after admission.The expression levels of TNF-αand NF-κB in AF and CON groups were detected by ELISA.The expression of NF-κB in peripheral blood lymphocytes of AF and CON groups was detected by Western blot.To study the relationship between TNF-α,NF-κB and atrial fibrillation.Results:The levels of TNF-αand NF-κB in AF group were higher than those in CON group,and the differences were statistically significant(P<0.05).nPAF group was higher than PAF group,the difference was statistically significant(P<0.05).The levels of TNF-α,NF-κB,LVEDD and LVEDV in PAF group were higher than those in CON group,but lower than those in nPAF group,the difference was statistically significant(P<0.05).The levels of LVEF and FS in PAF group were lower than CON group,but higher than nPAF group,with statistical significance(P<0.05).Pearson analysis showed that TNF-α,NF-κB were positively correlated with LVEDD and LVEDV in atrial fibrillation group compared with normal group(P<0.05),was negatively correlated with LVEF and FS(P<0.01).Conclusion:The levels of TNF-αand NF-κB in serum and NF-κB in peripheral blood lymphocytes were significantly increased in patients with atrial fibrillation.The occurrence and development of atrial fibrillation were related to NF-κB and TNF-α.展开更多
基金Science and Technology Research Project of Anhui Province(No.1804H08020246)Bengbu-Bengbu Medical College joint scientific and technological research(No.BYLK201814)+2 种基金2021 Graduate Research Innovation Plan of Bengbu Medical College(No.Byycx21062)The first batch of"512 Talent Cultivation Plan"middle-aged and young backbone teachers of Bengbu Medical College(No.BY51201214)The first batch of"512 Talent Cultivation Plan"middle-aged and young backbone teachers of Bengbu Medical College(No.BY51201315)。
文摘Objective:To detect the levels of TNF-αand NF-κB in serum and NF-κB in peripheral blood lymphocytes of patients with atrial fibrillation.To investigate the regularity and significance of its changes in patients with atrial fibrillation.Methods:Patients with atrial fibrillation hospitalized in our hospital from January 2020 to December 2021 were selected,including paroxysmal AF group(PAF group,n=75)and non-paroxysmal AF group(nPAF group,n=60),and healthy subjects were selected as the control group(CON group,n=107).Routine examination and color doppler echocardiography were improved after admission.The expression levels of TNF-αand NF-κB in AF and CON groups were detected by ELISA.The expression of NF-κB in peripheral blood lymphocytes of AF and CON groups was detected by Western blot.To study the relationship between TNF-α,NF-κB and atrial fibrillation.Results:The levels of TNF-αand NF-κB in AF group were higher than those in CON group,and the differences were statistically significant(P<0.05).nPAF group was higher than PAF group,the difference was statistically significant(P<0.05).The levels of TNF-α,NF-κB,LVEDD and LVEDV in PAF group were higher than those in CON group,but lower than those in nPAF group,the difference was statistically significant(P<0.05).The levels of LVEF and FS in PAF group were lower than CON group,but higher than nPAF group,with statistical significance(P<0.05).Pearson analysis showed that TNF-α,NF-κB were positively correlated with LVEDD and LVEDV in atrial fibrillation group compared with normal group(P<0.05),was negatively correlated with LVEF and FS(P<0.01).Conclusion:The levels of TNF-αand NF-κB in serum and NF-κB in peripheral blood lymphocytes were significantly increased in patients with atrial fibrillation.The occurrence and development of atrial fibrillation were related to NF-κB and TNF-α.
