Transcription factors(TFs)regulate diverse stress defensive-associated physiological processes and plant stress responses.We characterized TaNF-YB11,a gene of the NF-YB TF family in Triticum aestivum,in mediating plan...Transcription factors(TFs)regulate diverse stress defensive-associated physiological processes and plant stress responses.We characterized TaNF-YB11,a gene of the NF-YB TF family in Triticum aestivum,in mediating plant drought tolerance.TaNF-YB11 harbors the conserved domains specified by its NF-YB partners and targets the nucleus after the endoplasmic reticulum(ER)assortment.Yeast two-hybrid assay indicated the interactions of TaNF-YB11 with TaNF-YA2 and TaNF-YC3,two proteins encoded by genes in the NF-YA and NF-YC families,respectively.These results suggested that the heterotrimer established among them further regulated downstream genes at the transcriptional level.The transcripts of TaNF-YB11 were promoted in roots and leaves under a 27-h drought regime.Moreover,its upregulated expression levels under drought were gradually restored following a recovery treatment,suggesting its involvement in plant drought response.TaNF-YB11 conferred improved drought tolerance on plants;the lines overexpressing target gene displayed improved phenotype and biomass compared with wild type(WT)under drought treatments due to enhancement of stomata closing,osmolyte accumulation,and cellular reactive oxygen species(ROS)homeostasis.Knockdown expression of TaP5CS2,a P5CS family gene modulating proline biosynthesis that showed upregulated expression in drought-challenged TaNF-YB11 lines,alleviated proline accumulation of plants treated by drought.Likewise,TaSOD2 and TaCAT3,two genes encoding superoxide dismutase(SOD)and catalase(CAT)that were upregulated underlying TaNF-YB11 regulation,played critical roles in ROS homeostasis via regulating SOD and CAT activities.RNA-seq analysis revealed that numerous genes associated with processes of‘cellular processes',‘environmental information processing',‘genetic information processing',‘metabolism',and‘organismal systems'modified transcription under drought underlying control of TaNF-YB11.These results suggested that the TaNF-YB11-mediated drought response is possibly accomplished through the target gene in modifying gene transcription at the global level,which modulates complicated biological processes related to drought response.TaNF-YB11 is essential in plant drought adaptation and a valuable target for molecular breeding of drought-tolerant cultivars in T.aestivum.展开更多
Drought is a major abiotic stress that limits plant growth and development.Adaptive mechanisms have evolved to mitigate drought stress,including the capacity to adjust water loss rate and to modify the morphology and ...Drought is a major abiotic stress that limits plant growth and development.Adaptive mechanisms have evolved to mitigate drought stress,including the capacity to adjust water loss rate and to modify the morphology and structure of the epidermis.Here,we show that the expression of CmNF-YB8,encoding a nuclear factor Y(NF-Y)B-type subunit,is lower under drought conditions in chrysanthemum(Chrysanthemum morifolium).Transgenicchrysanthemumlinesinwhich transcript levels of CmNF-YB8 were reduced by RNA interference(CmNF-YB8-RNAi)exhibited enhanced drought resistance relative to control lines,whereas lines overexpressing CmNF-YB8(CmNF-YB8-OX)were less tolerant to drought.Compared to wild type(WT),CmNF-YB8-RNAi plants showed reduced stomatal opening and a thicker epidermal cuticle that correlated with their water loss rate.We also identified genes involved in stomatal adjustment(CBLinteracting protein kinase 6,CmCIPK6)and cuticle biosynthesis(CmSHN3)that are more highly expressed in CmNF-YB8-RNAi lines than in WT,CmCIPK6 being a direct downstream target of CmNF-YB8.Virus-induced gene silencing of CmCIPK6 or CmSHN3 in the CmNFYB8-RNAi background abolished the effects of CmNF-YB8-RNAi on stomatal closure and cuticle deposition,respectively.CmNF-YB8 thus regulates CmCIPK6 and CmSHN3 expression to alter stomatal movement and cuticle thickness in the leaf epidermis,thereby affecting drought resistance.展开更多
基金supported by the National Natural Science Foundation of China(31872869)the State Key Laboratory of North China Crop Improvement and Regulation(NCCIR2022ZZ-7)+2 种基金the National Key R&DProgram of China(SQ2022YFD1200002)the Science and Technology Planning Project of Hebei Province,China(216Z6401G)the Postgraduate Innovation Funding Project of Hebei Province,China(CXZZSS2021071)。
文摘Transcription factors(TFs)regulate diverse stress defensive-associated physiological processes and plant stress responses.We characterized TaNF-YB11,a gene of the NF-YB TF family in Triticum aestivum,in mediating plant drought tolerance.TaNF-YB11 harbors the conserved domains specified by its NF-YB partners and targets the nucleus after the endoplasmic reticulum(ER)assortment.Yeast two-hybrid assay indicated the interactions of TaNF-YB11 with TaNF-YA2 and TaNF-YC3,two proteins encoded by genes in the NF-YA and NF-YC families,respectively.These results suggested that the heterotrimer established among them further regulated downstream genes at the transcriptional level.The transcripts of TaNF-YB11 were promoted in roots and leaves under a 27-h drought regime.Moreover,its upregulated expression levels under drought were gradually restored following a recovery treatment,suggesting its involvement in plant drought response.TaNF-YB11 conferred improved drought tolerance on plants;the lines overexpressing target gene displayed improved phenotype and biomass compared with wild type(WT)under drought treatments due to enhancement of stomata closing,osmolyte accumulation,and cellular reactive oxygen species(ROS)homeostasis.Knockdown expression of TaP5CS2,a P5CS family gene modulating proline biosynthesis that showed upregulated expression in drought-challenged TaNF-YB11 lines,alleviated proline accumulation of plants treated by drought.Likewise,TaSOD2 and TaCAT3,two genes encoding superoxide dismutase(SOD)and catalase(CAT)that were upregulated underlying TaNF-YB11 regulation,played critical roles in ROS homeostasis via regulating SOD and CAT activities.RNA-seq analysis revealed that numerous genes associated with processes of‘cellular processes',‘environmental information processing',‘genetic information processing',‘metabolism',and‘organismal systems'modified transcription under drought underlying control of TaNF-YB11.These results suggested that the TaNF-YB11-mediated drought response is possibly accomplished through the target gene in modifying gene transcription at the global level,which modulates complicated biological processes related to drought response.TaNF-YB11 is essential in plant drought adaptation and a valuable target for molecular breeding of drought-tolerant cultivars in T.aestivum.
基金supported by the National Key Research and Development Program(2018YFD1000400)the National Natural Science Foundation of China(31572157 and31171990)+1 种基金the Beijing Municipal Science and Technology Project(Z191100008519007)the Construction of Beijing Science and Technology Innovation and Service Capacity in Top Subjects(CEFF-PXM2019_014207_000032)。
文摘Drought is a major abiotic stress that limits plant growth and development.Adaptive mechanisms have evolved to mitigate drought stress,including the capacity to adjust water loss rate and to modify the morphology and structure of the epidermis.Here,we show that the expression of CmNF-YB8,encoding a nuclear factor Y(NF-Y)B-type subunit,is lower under drought conditions in chrysanthemum(Chrysanthemum morifolium).Transgenicchrysanthemumlinesinwhich transcript levels of CmNF-YB8 were reduced by RNA interference(CmNF-YB8-RNAi)exhibited enhanced drought resistance relative to control lines,whereas lines overexpressing CmNF-YB8(CmNF-YB8-OX)were less tolerant to drought.Compared to wild type(WT),CmNF-YB8-RNAi plants showed reduced stomatal opening and a thicker epidermal cuticle that correlated with their water loss rate.We also identified genes involved in stomatal adjustment(CBLinteracting protein kinase 6,CmCIPK6)and cuticle biosynthesis(CmSHN3)that are more highly expressed in CmNF-YB8-RNAi lines than in WT,CmCIPK6 being a direct downstream target of CmNF-YB8.Virus-induced gene silencing of CmCIPK6 or CmSHN3 in the CmNFYB8-RNAi background abolished the effects of CmNF-YB8-RNAi on stomatal closure and cuticle deposition,respectively.CmNF-YB8 thus regulates CmCIPK6 and CmSHN3 expression to alter stomatal movement and cuticle thickness in the leaf epidermis,thereby affecting drought resistance.
