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孕期罗哌卡因麻醉通过NGF/ERK/CREB途径影响SD模型子代情绪及学习记忆能力
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作者 底妍 焦姗姗 +2 位作者 王金保 白喆 张在旺 《中国优生与遗传杂志》 2024年第8期1562-1569,共8页
目的探讨孕期罗哌卡因麻醉对子代情绪及学习记忆能力的影响及其潜在机制。方法本研究建立了怀孕大鼠模型,以研究在妊娠中期罗哌卡因麻醉后对后代的神经认知影响。在出生后第几天(P25~30)对其行为进行测试(Morris水迷宫和条件性恐惧实验... 目的探讨孕期罗哌卡因麻醉对子代情绪及学习记忆能力的影响及其潜在机制。方法本研究建立了怀孕大鼠模型,以研究在妊娠中期罗哌卡因麻醉后对后代的神经认知影响。在出生后第几天(P25~30)对其行为进行测试(Morris水迷宫和条件性恐惧实验)。此外,通过Nissl染色检测后代的海马形态(P30),并通过Golgi染色测定海马树突棘密度。通过蛋白质印迹法测定海马神经生长因子(NGF)、细胞外信号调节激酶(ERK)、磷酸化ERK(p-ERK),环磷酸腺苷反应元件结合(CREB)、p-CREB、突触素(SYP)、突触蛋白酶(SYN)和突触后密度-95(PSD95)的蛋白水平。此外,SCH772984(ERK抑制剂)用于评估PC12细胞中ERK途径的作用和潜在机制。结果结果显示罗哌卡因麻醉会导致长期神经认知功能障碍,降低树突旋转的密度,导致神经元损失,并下调海马中NGF、ERK、p-ERK、促分裂原和应激激活蛋白激酶(MSK)、CREB、p-CREB、SYP、SYN和PSD95的表达。结论在胎儿大脑发育期间,罗哌卡因诱导的母体麻醉会导致后代长期的神经认知功能障碍,这可能是通过抑制海马中的NGF/ERK/CREB通路而发生的。 展开更多
关键词 罗哌卡因 ngf/erk/creb途径 孕期 长期神经认知功能障碍
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Research progress of the role and mechanism of extracellular signal-regulated protein kinase 5(ERK5) pathway in pathological pain
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作者 Li-na YU Li-hong SUN +1 位作者 Min WANG Min YAN 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2016年第10期733-741,共9页
Extracellular signal-regulated protein kinase 5 (ERK5), also known as big mitogen-activated protein kinase 1 (MAPK1), is an important member of ERK family, which is a subfamily of the large MAPK family. ERK5 is ex... Extracellular signal-regulated protein kinase 5 (ERK5), also known as big mitogen-activated protein kinase 1 (MAPK1), is an important member of ERK family, which is a subfamily of the large MAPK family. ERK5 is expressed in many tissues, including the dorsal root ganglion (DRG) neurons and the spinal cord. In this review, we focus on elaborating ERK5-associated pathway in pathological pain, in which the ERK5/CREB (cyclic adenosine monophos- phate (cAMP)-response element-binding protein) pathway plays a crucial role in the transduction of pain signal and contributes to pain hypersensitivity. ERK5 activation in the spinal dorsal horn occurs mainly in microglia. The activation of ERK5 can be mediated by N-methyI-D-aspartate (NMDA) receptors. We also elaborate the relationship between ERK5 activation and nerve growth factor-tyrosine kinase A (NGF-TrkA), and the connection between ERK5 activation and brain-derived neurotrophic factor (BDNF) in pathological pain in detail. 展开更多
关键词 Extracellular signal-regulated protein kinase 5 erk5) Pain Cyclic adenosine monophosphate (cAMP)-response element-binding protein creb N-methyl-[^-aspartate (NMDA) Nerve growth factor ngf Brain-derived neurotrophic factor (BDNF)
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