Context: The functional activity of NK cells depends on the balance between the engagement of activating and inhibitory receptors on the cell surface with their ligands, which enables them to kill infected cells. Obje...Context: The functional activity of NK cells depends on the balance between the engagement of activating and inhibitory receptors on the cell surface with their ligands, which enables them to kill infected cells. Objectives: The aim of this study was to evaluate and compare expressions of selected activating and inhibitory receptors on stimulated NK cells in HIV-1 and HIV-2 infections. Methods: PBMCs were analysed for activating (NKp30, NKp44, NKp46) and inhibitory (CD158a, CD158b, p70) receptor expressions in 30 HIV-1, 30 HIV-2 and 30 HIV uninfected healthy control (HC) subjects by flow cytometry after stimulating with K562 cells. Results: There was an expression of other receptors following an already in vitro engagement of NK cells with K562 cells. Higher expression of the activating receptors, NKp44 (p = 0.029) and NKp46 (p = 0.032) on NK cells from HIV-2 compared to HIV-1 infected individuals but similar NKp30 expression (p = 0.980). The levels of expression of inhibitory receptor CD158a were similar between HIV-1 and HIV-2 infected subjects (p = 0.309) but there was significant up-regulation of inhibitory receptors p70 (p = 0.010) and CD158b (p = 0.05) in HIV-1 compared to HIV-2 subjects. Conclusion: Despite the in vitro engagement of NK cells with stimulating K562 cells, our data showed differential expressions of other selected activating and inhibitory receptors in HIV-1 and HIV-2 infected subjects.展开更多
Decidual lymphocytes may mediate fetal trophoblast recognition and regulate maternal immune reaction and play an essential role in the maintenance of normal pregnancy. The aim of this study was to compare the percenta...Decidual lymphocytes may mediate fetal trophoblast recognition and regulate maternal immune reaction and play an essential role in the maintenance of normal pregnancy. The aim of this study was to compare the percentage of T cells, natural killer （NK） cells and natural killer T （NKT） cells within decidual parietalis of normal pregnant controls （NP） and patients with intraheptic cholestasis of pregnancy （ICP）, and to investigate the production of interleukin-4 （IL-4）, interferon-γ （IFN-γ） in the culture supernatant of decidual parietalis mononuclear cells （DPMCs）. Compared with controls, the decidua parietalis from ICP were characterized with significant increased percentages of CD3^＋CD56^＋ cells, CD3^＋CD56^＋ cells, CD56^＋CD16＋ cells, CD56^＋CD16^＋ cells, CD56^＋NKG2D＋ ceils, and the significant decreased percentages of CD3^＋ cells, CD3^＋CD4^＋ cells. There were no differences found for the percentage of CD3^＋CD8^＋ cells, CD56^＋NKG2A^＋ cells between control and study group. In addition, the enhanced concentration of IFN-γ was presented in culture supernatant of DPMCs from ICP. It was suggested that the increased NK cells, NKT cells and the decreased T cells in the decidual parietalis and over-secretion of IFN-γ could be correlated with the pathophysiology of ICP patients. Cellular ＆ Molecular Immunology. 2007;4（1）：71-75.展开更多
文摘Context: The functional activity of NK cells depends on the balance between the engagement of activating and inhibitory receptors on the cell surface with their ligands, which enables them to kill infected cells. Objectives: The aim of this study was to evaluate and compare expressions of selected activating and inhibitory receptors on stimulated NK cells in HIV-1 and HIV-2 infections. Methods: PBMCs were analysed for activating (NKp30, NKp44, NKp46) and inhibitory (CD158a, CD158b, p70) receptor expressions in 30 HIV-1, 30 HIV-2 and 30 HIV uninfected healthy control (HC) subjects by flow cytometry after stimulating with K562 cells. Results: There was an expression of other receptors following an already in vitro engagement of NK cells with K562 cells. Higher expression of the activating receptors, NKp44 (p = 0.029) and NKp46 (p = 0.032) on NK cells from HIV-2 compared to HIV-1 infected individuals but similar NKp30 expression (p = 0.980). The levels of expression of inhibitory receptor CD158a were similar between HIV-1 and HIV-2 infected subjects (p = 0.309) but there was significant up-regulation of inhibitory receptors p70 (p = 0.010) and CD158b (p = 0.05) in HIV-1 compared to HIV-2 subjects. Conclusion: Despite the in vitro engagement of NK cells with stimulating K562 cells, our data showed differential expressions of other selected activating and inhibitory receptors in HIV-1 and HIV-2 infected subjects.
文摘Decidual lymphocytes may mediate fetal trophoblast recognition and regulate maternal immune reaction and play an essential role in the maintenance of normal pregnancy. The aim of this study was to compare the percentage of T cells, natural killer （NK） cells and natural killer T （NKT） cells within decidual parietalis of normal pregnant controls （NP） and patients with intraheptic cholestasis of pregnancy （ICP）, and to investigate the production of interleukin-4 （IL-4）, interferon-γ （IFN-γ） in the culture supernatant of decidual parietalis mononuclear cells （DPMCs）. Compared with controls, the decidua parietalis from ICP were characterized with significant increased percentages of CD3^＋CD56^＋ cells, CD3^＋CD56^＋ cells, CD56^＋CD16＋ cells, CD56^＋CD16^＋ cells, CD56^＋NKG2D＋ ceils, and the significant decreased percentages of CD3^＋ cells, CD3^＋CD4^＋ cells. There were no differences found for the percentage of CD3^＋CD8^＋ cells, CD56^＋NKG2A^＋ cells between control and study group. In addition, the enhanced concentration of IFN-γ was presented in culture supernatant of DPMCs from ICP. It was suggested that the increased NK cells, NKT cells and the decreased T cells in the decidual parietalis and over-secretion of IFN-γ could be correlated with the pathophysiology of ICP patients. Cellular ＆ Molecular Immunology. 2007;4（1）：71-75.
