In this study,ascorbyl palmitate(AP)incorporated in nanostructured lipid carriers(NLCs-AP)was fabricated using the hot-homogenization method.The amounts of AP,Tween 80,glyceryl stearate,and oleic acid were optimized b...In this study,ascorbyl palmitate(AP)incorporated in nanostructured lipid carriers(NLCs-AP)was fabricated using the hot-homogenization method.The amounts of AP,Tween 80,glyceryl stearate,and oleic acid were optimized by Box-Behnken Design(BBD)to obtain a high percentage of encapsulation efficiency and loading efficiency.Then,the optimum NLCs-AP,free-AP with rosemary essential oil(REO),NLCs-AP with REO,and tert-Butylhydroquinone(TBHQ)at a concentration of 75 ppm were added to camelina oil.Then peroxide value(PV),anisidine value(AV),TOTOX value(TV),oxidative stability using Rancimat,total phenol content(TPC),and antioxidant activity in camelina oil samples were evaluated during 90 days of storage at the ambient temper-ature.The optimum NLCs-AP had a particle size of 133.4 nm and a PDI of 0.29.The transmission electron microscopy(TEM)showed a spherical and smooth surface of the optimum NLCs-AP.The results of differential scanning calorimetry(DSC)and Fourier transfer spectroscopy infrared(FTIR)analyses implied that there was no interaction between AP and NLCs.The Korsmeyer-Peppas model was the best model for the evaluation of the release kinetics.The amounts of PV,AV,and TV of camelina oil containing the optimum NLCs-AP were slightly higher than the oil containing TBHQ.The highest oxidative protection,TPC,and antioxidant activity percentage were achieved in camelina oil containing NLCs-AP with REO.Conclusively,the optimum NLCs-AP had excellent potential for encapsulation of AP,and the mixture of REO and NLCs-AP could be applied for giving suitable oxidative stability in camelina oil.展开更多
神经元核内包涵体病(neuronal intranuclear inclusion body disease,NIID)是一种罕见的缓慢进展的可累及多系统的神经变性疾病,临床上异质性强,临床易误诊或漏诊。现报告1例经皮肤病理和基因检测明确的神经元核内包涵体病。患者,51岁,...神经元核内包涵体病(neuronal intranuclear inclusion body disease,NIID)是一种罕见的缓慢进展的可累及多系统的神经变性疾病,临床上异质性强,临床易误诊或漏诊。现报告1例经皮肤病理和基因检测明确的神经元核内包涵体病。患者,51岁,男,双上肢震颤9年余,认知下降4年,双眼视力下降4 d就诊。临床表现为帕金森病样表现、双侧瞳孔缩小、认知障碍等,头部磁共振DWI序列出现皮髓质交界区飘带征,皮肤病理活检小汗腺导管上皮及皮下脂肪组织内散在Ubiquitin及P62阳性的核内包涵体。基因检测:NOTCH2NLC中GGC重复扩增数>60。随访8个月,患者视力改善,其他症状无明显进展。该病例提示有必要提高对成人发病的神经元核内包涵体病的认识,通过影像学,皮肤活检以及NOTCH2NLC基因提高神经元核内包涵体病的诊断率。展开更多
文摘In this study,ascorbyl palmitate(AP)incorporated in nanostructured lipid carriers(NLCs-AP)was fabricated using the hot-homogenization method.The amounts of AP,Tween 80,glyceryl stearate,and oleic acid were optimized by Box-Behnken Design(BBD)to obtain a high percentage of encapsulation efficiency and loading efficiency.Then,the optimum NLCs-AP,free-AP with rosemary essential oil(REO),NLCs-AP with REO,and tert-Butylhydroquinone(TBHQ)at a concentration of 75 ppm were added to camelina oil.Then peroxide value(PV),anisidine value(AV),TOTOX value(TV),oxidative stability using Rancimat,total phenol content(TPC),and antioxidant activity in camelina oil samples were evaluated during 90 days of storage at the ambient temper-ature.The optimum NLCs-AP had a particle size of 133.4 nm and a PDI of 0.29.The transmission electron microscopy(TEM)showed a spherical and smooth surface of the optimum NLCs-AP.The results of differential scanning calorimetry(DSC)and Fourier transfer spectroscopy infrared(FTIR)analyses implied that there was no interaction between AP and NLCs.The Korsmeyer-Peppas model was the best model for the evaluation of the release kinetics.The amounts of PV,AV,and TV of camelina oil containing the optimum NLCs-AP were slightly higher than the oil containing TBHQ.The highest oxidative protection,TPC,and antioxidant activity percentage were achieved in camelina oil containing NLCs-AP with REO.Conclusively,the optimum NLCs-AP had excellent potential for encapsulation of AP,and the mixture of REO and NLCs-AP could be applied for giving suitable oxidative stability in camelina oil.
