目的:观察白介素-6(IL-6)对N-甲基-D-天门冬氨酸(NMDA)损伤小脑颗粒神经元NMDA受体亚单位1(NR1)和三磷酸肌醇受体1(IP3R1)蛋白表达的影响。方法:取出生后8 d SD大鼠小脑进行小脑颗粒神经元(CGNs)体外培养。在培养液中加入IL-6(40或120 n...目的:观察白介素-6(IL-6)对N-甲基-D-天门冬氨酸(NMDA)损伤小脑颗粒神经元NMDA受体亚单位1(NR1)和三磷酸肌醇受体1(IP3R1)蛋白表达的影响。方法:取出生后8 d SD大鼠小脑进行小脑颗粒神经元(CGNs)体外培养。在培养液中加入IL-6(40或120 ng/mL),培养8 d后,用NMDA(100μmol/L)损伤神经元30 min,建立神经元损伤模型。Western Blot法检测NR1和IP3R1蛋白的表达。结果:IL-6下调NR1的蛋白表达,并抑制NMDA诱导的IP3R1的蛋白表达增高。结论:IL-6通过抑制NMDA受体和IP3受体实现神经保护作用。展开更多
Objective: To explore the effects of the nerve growth factor (NGF) on N methyl D asparate receptor 1 (NMDAR 1) after spinal cord injury. Methods: Spinal cord injury of Wistar rats was performed with Allens method by a...Objective: To explore the effects of the nerve growth factor (NGF) on N methyl D asparate receptor 1 (NMDAR 1) after spinal cord injury. Methods: Spinal cord injury of Wistar rats was performed with Allens method by a 10 g×2.5 cm impact on the posterior T8 spinal cord. NGF was given to the rats of the treatment group via subarachnoid space tube at once, 2, 4, 8, 12 and 24 hours after spinal cord injury, respectively. The expression of NMDAR1 mRNA in spinal cord was detected by in situ hybridization. Results: Rare expression sequence of NMDAR1 mRNA was found in rat spinal cord of the normal group. A strong expression sequence of NMDAR1 mRNA was found in rat spinal cord of the normal saline group. The expression of NMDAR1 mRNA in the NGF group was significantly decreased as compared with that in the normal saline group (P= 0.01 ).Conclusions: NGF can relieve damage of injured spinal cord by prohibiting the expression of NMDAR1 mRNA.展开更多
Objective： To explore the molecular biological mechanism of acupuncture in intervening visual deprivation. Methods： Forty-eight 2-week old Wistar rats were randomly divided into a normal group, a model group, and 6 ...Objective： To explore the molecular biological mechanism of acupuncture in intervening visual deprivation. Methods： Forty-eight 2-week old Wistar rats were randomly divided into a normal group, a model group, and 6 acupuncture groups （group C1： acupuncture at the unaffected side in early stage; group C2： acupuncture at the affected side in early stage; group DI： acupuncture at the unaffected side in mid-stage; group D2： acupuncture at the affected side in mid-stage; group El： acupuncture at the unaffected side in late stage; group E2： acupuncture at the affected side in late stage） by the random number table, 6 rats in each group. Rats in the normal group didn＇t receive any interventions. The rat model of deprivation amblyopia was established by unilateral eyelid suture in the model group and each acupuncture group After successful modeling, rats in model group didn＇t receive any treatments; rats in the acupuncture groups received acupuncture intervention which began respectively on the 3rd, 12th and 21st day after modeling. Pattern visual evoked potential （P-VEP） and N-methy D-aspartatreceptor-1 （NMDAR1） mRNA expression in visual cortex area 17 were detected at the end of acupuncture intervention in each group. Results： After the intervention, the P-VEP waveform was significantly changed, with a significantly delayed Pzoo value （P〈0.01） and significantly decreased amplitude of N45-Ploo in the model group versus the normal group （P〈O.01）; the P-VEP waveform was significantly improved, with obviously earlier P10o （P〈0.01） and increased amplitude of N4s-Ploo （P〈O.05） in each acupuncture group versus the model group. The improvement effect of acupuncture on the P-VEP waveform in group C1 and C2 was more significant than that in group D1, D2, E1 and E2. The expression of NMDAR1 mRNA of the rat visual cortex area 17 in the model group was significantly lower than that in the normal group （P〈0.01）; and the expression of NMDAR1 mRNA in the visual cortex area 17 of each acupuncture group was significantly higher than that in the model group （P〈0.05）; the effect of acupuncture on NMDAR1 mRNA expression in group C1 and C2 was more significant than that in group D1, D2, E1 and E2; and the effect of acupuncture on NMDAR1 mRNA expression was better in group C2 than in group C1 （P〈O.05）; there was no significant difference in the expression of NMDAR1 mRNA between group D1 and D2, neither between E1 and E2 （P〉0.05）. Conclusion： P-VEP waveform is abnormal and NMDAR1 mRNA expression in visual cortex area 17 is decreased in rats with monocularly-deprived amblyopia. Acupuncture in the sensitive period can significantly regulate the abnormal P-VEP waveform and the down-regulate the NMDAR1 mRNA expression of the visual cortex of rats with monocularly-deprived amblyopia. Early treatment in the sensitive period should be the key to obtaining the curative effect.展开更多
脑卒中是临床常见病、多发病,是目前危害人类健康最重要的疾病之一,且发病率、病死率、致残率呈逐年上升趋势,然而,脑卒中后神经功能损伤机制尚未明确,仍缺乏有效的治疗手段,寻找神经保护机制,对治疗脑卒中具有重要意义。目前有研究发现...脑卒中是临床常见病、多发病,是目前危害人类健康最重要的疾病之一,且发病率、病死率、致残率呈逐年上升趋势,然而,脑卒中后神经功能损伤机制尚未明确,仍缺乏有效的治疗手段,寻找神经保护机制,对治疗脑卒中具有重要意义。目前有研究发现,脑卒中时死亡相关蛋白激酶1(death-associated protein kinase 1,DAPK1)作为一种特异性的细胞死亡信号被活化,与神经元突触外N-甲基-D-天冬氨酸(N-methyl-D-aspartate,NMDA)受体结合引发神经元缺血性坏死,若将DAPK1从NMDA受体复合物上解离下来可保护神经元免受损伤,这成为治疗脑卒中的一个新靶点。展开更多
文摘Objective: To explore the effects of the nerve growth factor (NGF) on N methyl D asparate receptor 1 (NMDAR 1) after spinal cord injury. Methods: Spinal cord injury of Wistar rats was performed with Allens method by a 10 g×2.5 cm impact on the posterior T8 spinal cord. NGF was given to the rats of the treatment group via subarachnoid space tube at once, 2, 4, 8, 12 and 24 hours after spinal cord injury, respectively. The expression of NMDAR1 mRNA in spinal cord was detected by in situ hybridization. Results: Rare expression sequence of NMDAR1 mRNA was found in rat spinal cord of the normal group. A strong expression sequence of NMDAR1 mRNA was found in rat spinal cord of the normal saline group. The expression of NMDAR1 mRNA in the NGF group was significantly decreased as compared with that in the normal saline group (P= 0.01 ).Conclusions: NGF can relieve damage of injured spinal cord by prohibiting the expression of NMDAR1 mRNA.
基金supported by National Natural Science Foundation of China,No.81260560~~
文摘Objective： To explore the molecular biological mechanism of acupuncture in intervening visual deprivation. Methods： Forty-eight 2-week old Wistar rats were randomly divided into a normal group, a model group, and 6 acupuncture groups （group C1： acupuncture at the unaffected side in early stage; group C2： acupuncture at the affected side in early stage; group DI： acupuncture at the unaffected side in mid-stage; group D2： acupuncture at the affected side in mid-stage; group El： acupuncture at the unaffected side in late stage; group E2： acupuncture at the affected side in late stage） by the random number table, 6 rats in each group. Rats in the normal group didn＇t receive any interventions. The rat model of deprivation amblyopia was established by unilateral eyelid suture in the model group and each acupuncture group After successful modeling, rats in model group didn＇t receive any treatments; rats in the acupuncture groups received acupuncture intervention which began respectively on the 3rd, 12th and 21st day after modeling. Pattern visual evoked potential （P-VEP） and N-methy D-aspartatreceptor-1 （NMDAR1） mRNA expression in visual cortex area 17 were detected at the end of acupuncture intervention in each group. Results： After the intervention, the P-VEP waveform was significantly changed, with a significantly delayed Pzoo value （P〈0.01） and significantly decreased amplitude of N45-Ploo in the model group versus the normal group （P〈O.01）; the P-VEP waveform was significantly improved, with obviously earlier P10o （P〈0.01） and increased amplitude of N4s-Ploo （P〈O.05） in each acupuncture group versus the model group. The improvement effect of acupuncture on the P-VEP waveform in group C1 and C2 was more significant than that in group D1, D2, E1 and E2. The expression of NMDAR1 mRNA of the rat visual cortex area 17 in the model group was significantly lower than that in the normal group （P〈0.01）; and the expression of NMDAR1 mRNA in the visual cortex area 17 of each acupuncture group was significantly higher than that in the model group （P〈0.05）; the effect of acupuncture on NMDAR1 mRNA expression in group C1 and C2 was more significant than that in group D1, D2, E1 and E2; and the effect of acupuncture on NMDAR1 mRNA expression was better in group C2 than in group C1 （P〈O.05）; there was no significant difference in the expression of NMDAR1 mRNA between group D1 and D2, neither between E1 and E2 （P〉0.05）. Conclusion： P-VEP waveform is abnormal and NMDAR1 mRNA expression in visual cortex area 17 is decreased in rats with monocularly-deprived amblyopia. Acupuncture in the sensitive period can significantly regulate the abnormal P-VEP waveform and the down-regulate the NMDAR1 mRNA expression of the visual cortex of rats with monocularly-deprived amblyopia. Early treatment in the sensitive period should be the key to obtaining the curative effect.
文摘脑卒中是临床常见病、多发病,是目前危害人类健康最重要的疾病之一,且发病率、病死率、致残率呈逐年上升趋势,然而,脑卒中后神经功能损伤机制尚未明确,仍缺乏有效的治疗手段,寻找神经保护机制,对治疗脑卒中具有重要意义。目前有研究发现,脑卒中时死亡相关蛋白激酶1(death-associated protein kinase 1,DAPK1)作为一种特异性的细胞死亡信号被活化,与神经元突触外N-甲基-D-天冬氨酸(N-methyl-D-aspartate,NMDA)受体结合引发神经元缺血性坏死,若将DAPK1从NMDA受体复合物上解离下来可保护神经元免受损伤,这成为治疗脑卒中的一个新靶点。
